Microscopy nerd alert: two scopes in California can now do phase contrast cryoEM - potentially fixing the tech's very low signal/noise ratio. The phase filter they used is a laser. Which is also the brightest thing in the solar system (outside of the inside of the sun). cen.acs.org/analytical-c...

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

Better vitrification = better cryo-ET. We've opened an RFA for two-year grants to advance vitrification techniques for biological samples—welcoming applications from researchers in heat transfer, cryogenics, and materials science. Apply here: bit.ly/4uip6eJ

Excited to share our work uncovering SNOR, a ribosome-associated factor that promotes translation restart after dormancy published in Nature!! This was, as usual, a wonderful collaborative effort between our group and the Mattei Lab @simonemattei.bsky.social @embl.org www.nature.com/articles/s41...

First PROTAC gains FDA approval, bolstering targeted protein degradation and induced proximity ambitions www.nature.com/articles/d41...

Today we're launching the Open Source for Science Fund, a new multi-donor philanthropic fund by Renaissance Philanthropy, seeded by @biohub.org and @wellcometrust.bsky.social, with support from @kavlifoundation.org and @researchsoftware.bsky.social 🧵 os4science.org/news/open-so...

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Today we're announcing the Virtual Biology Initiative—a major new commitment to build the data foundation biology needs to power the next generation of AI models. Next-gen imaging. Molecular engineering tools. Open data infrastructure for the entire scientific community. Read more: bit.ly/3ONTkHO

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

Excited to share our paper about copick, a dataset API and toolkit for collaborative annotation and analysis of #cryoET data! Whether you're picking particles or curating segmentations, copick reduces friction and brings #OME-Zarr to cryoET without breaking pipelines. 🧵👇 doi.org/10.1002/pro.70578

Check out our new release on BioRxiv co-lead by @pm-mueller.bsky.social 👉 rb.gy/lnrizk special🙏 to Severine Kunz #MDC @leventallab.bsky.social @andimicroscopy.bsky.social @ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM Are the days of discovering new cellular structures over?? /1

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

💪 NEW VIDEO: Flying over the A-band of an atomic-scale model of a vertebrate muscle sarcomere. Let's explore the molecular mechanics that make your muscles work. Rendered using @bradyajohnston.bsky.social 's molecular nodes Model based on the incredible work of the @raunser-lab.bsky.social lab

www.nature.com/articles/s41... 🧪. DeepMind AlphaGenome, trained on 1 Mb of DNA sequence from human and mouse and predicts thousands of functional genomic tracks including gene expression, transcription initiation, chromatin accessibility, histone modifications, transcription factor binding etc

Excited to see how #teamtomo #AIxBio use these datasets!

New on the CryoET Data Portal: ~27,000 tomograms of affinity-captured lysosomes from HEK293T cells across 4 physiological states. Includes raw data, AreTomo3 reconstructions & Membrain-Seg annotations. Openly available for reuse! cryoetdataportal.czscience.com/depositions/...

Huge congrats to @manuelthery.bsky.social and all the Cytomorpho lab. The "Living Architectures" performance at @museeorsay.bsky.social was breathtakingly beautiful

IsoNet2 determines cellular structures at submolecular resolution without averaging [new] 3D cryo-ET recon: self-sup. DL refines struct., revealing details in situ.

#LivingArchitectures We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay. www.musee-orsay.fr/fr/agenda/ev... Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night. -> Two shows on the 24th and 25th of January.

Hi #teamtomo! Want to use OME-Zarr for your tilt series but can't integrate it with your existing sub-tomogram averaging pipelines? Try zarr-particle-tools - RELION-style extraction & reconstruction built for OME-Zarr-based workflows! 📦 pip install zarr-particle-tools 🔗 github.com/czimagingins...

Muyuan Chen has turned structural biology into an immersive experience with his new video game Meowtabolism, now available on Steam. Try the demo here: store.steampowered.com/app/4045010/... Give Muyuan feedback: steamcommunity.com/app/4045010 #ScienceGaming #StructuralBiology #CryoEM #STEMOutreach

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET https://www.biorxiv.org/content/10.1101/2025.09.16.676641v1

NEW from me at STAT: We've learned that DeepMind is going to fund CASP, the protein structure competition that brought Google DeepMind's #AlphaFold to prominence (and a Nobel Prize), as its NIH funding runs out. More @statnews.com: www.statnews.com/2025/07/21/c... 🖥️🩺🧪🧬 #bioML

A future perspective article on the next 10 years in microbial molecular biology and physiology is online now. journals.asm.org/doi/10.1128/... 🧵👇

www.nature.com/articles/d41... #heartmusclecells :)

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

🎉 Congratulations to Wolfgang Baumeister @maxplanck.de, awarded the 2025 #ShawPrize in Life Science & Medicine for pioneering work in cryo-ET! Learn more 👉 europa.eu/!JBXDBG #FrontierResearch #ERCSyG #CryoET #StructuralBiology #shawprize2025

I was awestruck by the work I saw presented at the CZI Imaging Institute today. They are pursuing difficult problems head on with great ideas and the right people, and it's paying off in progress. In situ structural biology is going to be a solved problem. A million thanks to my host Shawn Zheng.

I’m delighted that we could contribute the #cryoEM data to this incredible antiviral discovery project from the group of @neytsjohan.bsky.social 💊 "A coronavirus assembly inhibitor that targets the viral membrane protein" www.nature.com/articles/s41... A huge congrats to everyone involved! 🎉

A new paper from our lab is available at doi.org/10.1101/2025.... The work describes how ArsA, which is involved in arsenic detoxification, utilizes nucleotides to drive dramatic conformational changes—multiple EM structures of a 64kDa protein. Congratulations to Shivansh Mahajan.

The Mechanisms and Innovations in Cardiovascular Disease T32 Training Grant is currently recruiting PhD and MD scientists to join its postdoctoral training fellowship. To apply, please follow this link: stanforduniversity.qualtrics.com/jfe/form/SV_....

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoem

www.biorxiv.org/content/10.1... #teamtomo #cryoET

Notre Dame is so back

Watch out for an upcoming bluetorial/tweetorial on this work from the amazing @alisiafadini.bsky.social! www.biorxiv.org/content/10.1...

It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.

Deeply saddened by the passing of Prof. Mike Sheetz, a true leader in Mechanobiology. His groundbreaking work on Kinesin, membrane organization, and mechanotransduction has left an indelible mark. A tremendous loss to science. #Mechanobiology #Science #LaskerAward

A special Bluetorial about one of my mentors Ruth Kirschstein Among Ruth Kirschstein’s many contributions to American science was the development and expansion of programs intended to increase participation of people from groups underrepresented in biomedical research.

@caltech.edu asked Dr. Pamela Bjorkman "if overusing antibiotics can be harmful. Should we worry about overusing vaccines too?" The short answer is no. Swipe to learn more from Caltech's Pamela Bjorkman. #AskCaltech at caltech.edu/AskCaltech. 1/2

Let me give a concrete example of the importance of applications with high risk-high reward as well as the role of NIH staff in taking risks. Decades ago, a physical chemist proposed ideas about doing mass spectrometry on proteins (when this was deemed probably impossible). 1/n

I've been hoping that someone would attempt to train a structure prediction model against experimental data (rather than 3D coordinates), and hopefully this is a step in that direction SFCalculator: connecting deep generative models and crystallography doi.org/10.1101/2025...

You want to build an apparatus for time-resolved cryo-EM? Easy: bio-protocol.org/en/bpdetail?...

The info session for our FutureHouse fellowship is TOMORROW, Tuesday, 9:00am Pacific time. Come get all your questions answered! Details are on our website: www.futurehouse.org/fellowship Now for an AI generated image that we present as an offering to the Algorithms, may they Boost our Post.

The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!

Thanks to those who’ve reached out the last few days. I am grateful that my family and home are safe. Many of my friends and colleagues have lost homes. If you want to help Caltech/JPL faculty/students/staff, a relief fund has been established: giving.caltech.edu/areas-to-sup...

After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service. @jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases Details: cdn.rcsb.org/rcsb-pd...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇