IsoNet2 determines cellular structures at submolecular resolution without averaging [new] 3D cryo-ET recon: self-sup. DL refines struct., revealing details in situ.

#LivingArchitectures We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay. www.musee-orsay.fr/fr/agenda/ev... Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night. -> Two shows on the 24th and 25th of January.

Hi #teamtomo! Want to use OME-Zarr for your tilt series but can't integrate it with your existing sub-tomogram averaging pipelines? Try zarr-particle-tools - RELION-style extraction & reconstruction built for OME-Zarr-based workflows! 📦 pip install zarr-particle-tools 🔗 github.com/czimagingins...

Muyuan Chen has turned structural biology into an immersive experience with his new video game Meowtabolism, now available on Steam. Try the demo here: store.steampowered.com/app/4045010/... Give Muyuan feedback: steamcommunity.com/app/4045010 #ScienceGaming #StructuralBiology #CryoEM #STEMOutreach

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET https://www.biorxiv.org/content/10.1101/2025.09.16.676641v1

NEW from me at STAT: We've learned that DeepMind is going to fund CASP, the protein structure competition that brought Google DeepMind's #AlphaFold to prominence (and a Nobel Prize), as its NIH funding runs out. More @statnews.com: www.statnews.com/2025/07/21/c... 🖥️🩺🧪🧬 #bioML

A future perspective article on the next 10 years in microbial molecular biology and physiology is online now. journals.asm.org/doi/10.1128/... 🧵👇

www.nature.com/articles/d41... #heartmusclecells :)

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

🎉 Congratulations to Wolfgang Baumeister @maxplanck.de, awarded the 2025 #ShawPrize in Life Science & Medicine for pioneering work in cryo-ET! Learn more 👉 europa.eu/!JBXDBG #FrontierResearch #ERCSyG #CryoET #StructuralBiology #shawprize2025

I was awestruck by the work I saw presented at the CZI Imaging Institute today. They are pursuing difficult problems head on with great ideas and the right people, and it's paying off in progress. In situ structural biology is going to be a solved problem. A million thanks to my host Shawn Zheng.

I’m delighted that we could contribute the #cryoEM data to this incredible antiviral discovery project from the group of @neytsjohan.bsky.social 💊 "A coronavirus assembly inhibitor that targets the viral membrane protein" www.nature.com/articles/s41... A huge congrats to everyone involved! 🎉

A new paper from our lab is available at doi.org/10.1101/2025.... The work describes how ArsA, which is involved in arsenic detoxification, utilizes nucleotides to drive dramatic conformational changes—multiple EM structures of a 64kDa protein. Congratulations to Shivansh Mahajan.

The Mechanisms and Innovations in Cardiovascular Disease T32 Training Grant is currently recruiting PhD and MD scientists to join its postdoctoral training fellowship. To apply, please follow this link: stanforduniversity.qualtrics.com/jfe/form/SV_....

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoem

www.biorxiv.org/content/10.1... #teamtomo #cryoET

Notre Dame is so back

Watch out for an upcoming bluetorial/tweetorial on this work from the amazing @alisiafadini.bsky.social! www.biorxiv.org/content/10.1...

My heart goes out to all my NIH people. Unbelievable.

It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.

Deeply saddened by the passing of Prof. Mike Sheetz, a true leader in Mechanobiology. His groundbreaking work on Kinesin, membrane organization, and mechanotransduction has left an indelible mark. A tremendous loss to science. #Mechanobiology #Science #LaskerAward

A special Bluetorial about one of my mentors Ruth Kirschstein Among Ruth Kirschstein’s many contributions to American science was the development and expansion of programs intended to increase participation of people from groups underrepresented in biomedical research.

@caltech.edu asked Dr. Pamela Bjorkman "if overusing antibiotics can be harmful. Should we worry about overusing vaccines too?" The short answer is no. Swipe to learn more from Caltech's Pamela Bjorkman. #AskCaltech at caltech.edu/AskCaltech. 1/2

Let me give a concrete example of the importance of applications with high risk-high reward as well as the role of NIH staff in taking risks. Decades ago, a physical chemist proposed ideas about doing mass spectrometry on proteins (when this was deemed probably impossible). 1/n

I've been hoping that someone would attempt to train a structure prediction model against experimental data (rather than 3D coordinates), and hopefully this is a step in that direction SFCalculator: connecting deep generative models and crystallography doi.org/10.1101/2025...

You want to build an apparatus for time-resolved cryo-EM? Easy: bio-protocol.org/en/bpdetail?...

The info session for our FutureHouse fellowship is TOMORROW, Tuesday, 9:00am Pacific time. Come get all your questions answered! Details are on our website: www.futurehouse.org/fellowship Now for an AI generated image that we present as an offering to the Algorithms, may they Boost our Post.

The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!

Thanks to those who’ve reached out the last few days. I am grateful that my family and home are safe. Many of my friends and colleagues have lost homes. If you want to help Caltech/JPL faculty/students/staff, a relief fund has been established: giving.caltech.edu/areas-to-sup...

After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service. @jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases Details: cdn.rcsb.org/rcsb-pd...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

Curious to learn more, but need a guide to this interdisciplinary study? I've got you... 1n

4/n) We discover a sperm-specific axoneme-tethered TRiC chaperone that likely contributes to constructing or maintaining the long flagella of mammalian sperm.

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii biorxiv.org/cgi/content/short/2024.12.28.630444v1

A look at how some drug molecules are peculiarly slow-moving once they get into the cell:

Another great thread; this time on the classic “Stryer” biochemistry textbook. Incidentally, I’m currently teaching a biochemistry course using the 10th edition. The quote below is golden

We discovered 3 RNA families that form homo-oligomeric RNA-only complexes! Studying nature continues to expand my wildest imagination. Read about it: doi.org/10.1101/2024... Vivian Wu, Svetlana Shabalina, Hyunbin Lee, Grace Nye, Eugene Koonin, Alex Gao, Rhiju Das, Wah Chiu

A really exciting day for the scientists, inventors, biotechnologists, and, most importantly, patients who made this possible.

Naturally ornate RNA-only complexes revealed by cryo-EM https://www.biorxiv.org/content/10.1101/2024.12.08.627333v1

Sharing highly encouraged: My lab has an opening for a postdoc, to study structural biology, biophysics and pharmacology of GPCRs in neuro- and immunobiology. Come join our diverse and collaborative team in sunny SoCal - please reach out with any questions! dornsife.usc.edu/gati/

Very excited to present the structural and mechanistic basis for stress response silencing by the monster E3 ligase SIFI. www.biorxiv.org/content/10.1...

CASP would not be possible without the great work of our expert assessors. Here is the list of #CASP16 assessors for the different categories:

We have a great PhD program across many disciplines, including #proteinengineering, #directedevolution, #machinelearning, #biocatalysis, #enzymes, and much more.

It took us seven years, but our collaboration with @profmattjohnson.bsky.social on the molecular architecture of plant thylakoids is finally online. Check it out! #PlantScience #TeamTomo 🧪🔬🧶🧬🌾

Faster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵 💾 github.com/SBQ-1999/Cry...

My favorite NPR story blog - “Goats and Soda” sent a writer and photographer to a rural school in India; an early adopter of Foldscope. A wonderful photo story of kids “reading nature”. In this current challenging climate - the kids show us the way with joy and wonder. www.npr.org/sections/goa...