woj_wie
@wojwie.bsky.social
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Photosynthesis and cryoEM at Biozentrum, Basel.
reposted by
woj_wie
Biozentrum, University of Basel
4 days ago
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging
www.biozentrum.unibas.ch/open-positio...
#biozentrum
#University
#Basel
#Professor
#Structural
#Biology
#Biophysics
#Biological
#Imaging
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woj_wie
Ben Engel
8 days ago
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with
@profmattjohnson.bsky.social
to dive into spinach chloroplasts with
#CryoET
❄️🔬. Curious? ..Read on!
#TeamTomo
#PlantScience
🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵
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Sven Klumpe
about 2 months ago
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here:
meetings.embo.org/event/26-in-...
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woj_wie
Biozentrum, University of Basel
2 months ago
Apply now for the prestigious and independent
#BiozentrumPhDFellowships
. Great science. Unique rotation-based selection of research group and other incentives. The summer call is open until October 12, 2025.
bit.ly/4caiqqX
@biozentrum.unibas.ch
@unibas.ch
#fellowship
#PhD#Switzerland
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Biozentrum PhD Fellowships
Share your passion for life sciences. If you are talented and highly motivated, want to broaden your horizons and are interested in a wide range of research topics, apply for one of the sought after B...
https://bit.ly/4caiqqX
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woj_wie
Manon Demulder
3 months ago
Our first DinoSphere seminar is this Tuesday, 1 July at 9 AM CEST! A good way to start the day if you're in Europe ☕ We will hear from Yong Heng Phua (OIST) and Karel Mocaer (Heidelberg) 🔗 Zoom link on our website:
tinyurl.com/4duvwtvp
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DinoSphere - Online talks
Video credits: Liz Cooney
https://tinyurl.com/4duvwtvp
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woj_wie
Florian Wollweber
3 months ago
After 5 amazing years
@pilhoferlab.bsky.social
I'm starting my lab
@embl.org
Grenoble this November
embl.org/wollweber
We'll image Asgard archaea and many other strange microbes (
#archaeasky
,
#protistsonsky
..) to understand eukaryogenesis First job ad:
#teamtomo
scientist!🔬❄️
tinyurl.com/2rdu2ze6
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woj_wie
Manon Demulder
4 months ago
We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST) 📅 1 July 9AM CEST 🔗
tinyurl.com/4mjaverj
Spread the word!
@protistwtmostest.bsky.social
@ehehenberger.bsky.social
@chandnibhickta.bsky.social
&Norico Yamada
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woj_wie
Matt Johnson
4 months ago
Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol 🌞🌱
rdcu.be/em1lz
Hopefully a useful primer for Postgrad and undergrad students
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Structure, regulation and assembly of the photosynthetic electron transport chain
Nature Reviews Molecular Cell Biology - The electron transfer chain in chloroplast thylakoid membranes uses solar energy to produce NADPH and ATP, which power carbon fixation into biomass. This...
https://rdcu.be/em1lz
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woj_wie
George Currie
5 months ago
Does publishing serve science or is science serving publishing? Damian Pattinson and I (
@elife.bsky.social
) argue scientific publishing has evolved into a system that, rather than facilitate scholarly communication, distorts and dictates it.
onlinelibrary.wiley.com/doi/10.1002/...
#OpenScience
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woj_wie
Philippe Van der Stappen
5 months ago
Hey
#TeamTomo
, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why
@florentwaltz.bsky.social
and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET
#CryoEM
🔬🧪
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Welcome to TomoGuide
A step-by-step Cryo-ET guide
https://tomoguide.github.io/
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woj_wie
Florent Waltz
5 months ago
You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ?
@phaips.vd.st
and I have a website for you!
tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !
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woj_wie
Lorenz Lamm
5 months ago
🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in
#CryoET
: segmentation, particle picking, and spatial statistics. 🔗 Preprint:
doi.org/10.1101/2024...
🔗 Code:
github.com/CellArchLab/...
🧵(1/6)
#TeamTomo
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woj_wie
Philipp Erdmann
5 months ago
Hey Everyone!❄️🔬 We have two fully funded PhD positions
@humantechnopole.bsky.social
this year! Help us understand better how one of the main stress regulators - the nucleolus - integrates synaptic inputs!🧠Find all the calls - also from my amazing colleagues - here:
www.semm.it/education/ho...
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woj_wie
Florian Wollweber
7 months ago
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum:
www.cell.com/cell/fulltex...
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woj_wie
Florent Waltz
7 months ago
The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in
@science.org
🎉 You can find the full story here
www.science.org/doi/10.1126/...
@verenaresch.bsky.social
did a wonderful job on the cover and animation, she really brought it to life 👩🎨
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woj_wie
Katharina Geißler
7 months ago
Check out
@deglushk.bsky.social
's method to measure membrane thickness in cryo electron tomograms!
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woj_wie
Richard Sever
7 months ago
Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's not a new preprint server; it's dedicated organization to oversee the servers]
openrxiv.org
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Homepage - openRxiv
openRxiv is an independent non-profit, the new organizational home for bioRxiv and medRxiv, enabling researchers to instantly share groundbreaking findings with the global scientific community.
https://openrxiv.org/
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woj_wie
Carl T. Bergstrom
7 months ago
Let's talk about this Nature piece in more detail. I've rarely read something so anti-scientific anywhere short of the National Review.
www.nature.com/articles/d41...
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Three AI-powered steps to faster, smarter peer review
Tired of spending countless hours on peer reviews? An AI-assisted workflow could help.
https://www.nature.com/articles/d41586-025-00526-0
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woj_wie
Sven Klumpe
7 months ago
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in
@cellcellpress.bsky.social
www.cell.com/cell/fulltex...
. What’s new?
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woj_wie
Ricardo D. Righetto
7 months ago
To all
#TeamTomo
#CryoET
and
#Chlamydomonas
aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽💻
www.ebi.ac.uk/empiar/EMPIA...
A little thread about what's new... 🧵 1/n
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woj_wie
Lorna Malone
8 months ago
📢 Registration is now open for 'Wellcome-MRC Cryo-EM in Structural Biology 2025'! 📅 Join us online March 3-7 at
@diamondlightsource.bsky.social
@universityofleeds.bsky.social
for lectures & demos in
#CryoEM
&
#CryoET
: sample prep, SPA, model building & more!🧬 🔗
www.diamond.ac.uk/Instruments/...
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Wellcome-MRC Cryogenic Electron Microscopy in Structural Biology 2025 - - Diamond Light Source
eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Fac...
https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC/Training/Courses-and-workshops/2025-EventsCW/Cryogenic-Electron-Microscopy-in-Structural-Biology-2025.html
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woj_wie
Marcel Dickmanns | 🌿❄️🔬
8 months ago
Our methods paper made it to bioRxiv:
doi.org/10.1101/2025...
Full
#cryoET
workflows for
#plant
tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5
#teamtomo
#PlantScience
#Physcomitrium
#Arabidopsis
#cryoEM
#CLEM
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woj_wie
Sven Klumpe
8 months ago
Extremely excited to be joining the vibrant community of the Vienna BioCenter to build up cryo-ET and cryo-FIB milling on campus soon!
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woj_wie
Sjors Scheres
8 months ago
Give control to shareholders, and enshittification will happen, where a relentless drive for growth will reduce quality for users. It would be cheaper and better to keep companies away from control over scientific publishing. Scientists do most of the work anyway. Look at
@elife.bsky.social
!
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woj_wie
Bianca Introini
8 months ago
I am very happy to share our latest work on the detailed cryo-EM structure of the NDH-PSI supercomplex of spinach. Thanks to Werner Kühlbrandt, Alex Hahn and
@mpibp.bsky.social
! 🍃❄️🔬 You can find the paper in open access in Nature Structural and Molecular Biology:
www.nature.com/articles/s41...
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Cryo-EM structure of the NDH–PSI–LHCI supercomplex from Spinacia oleracea - Nature Structural & Molecular Biology
Here, the authors report the structure of the nicotinamide adenine dinucleotide phosphate dehydrogenase–photosystem I–light-harvesting complex I supercomplex, providing insights into subunit interacti...
https://www.nature.com/articles/s41594-024-01478-1
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woj_wie
Yannick Schwab
8 months ago
Another reminder, we are soon reaching the max number of participants so now is a good time to register
#volumeEM
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woj_wie
SBGrid Consortium
8 months ago
In case you missed our first webinar of 2025, it is now available. Lorenz Lamm from MUDS - Munich School for Data Science presented MemBrainv2- an end-to-end tool for the analysis of membranes in cryo-electron tomography.
https://buff.ly/4hutFwf
#SBGridwebinars
#ScienceMatters
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MemBrainv2
SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI J...
https://buff.ly/4hutFwf
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woj_wie
Richard Sever
9 months ago
Announcing the new bioRxiv dashboard widget - it's now easier to when articles have comments, peer reviews, community discussion, media coverage, videos, and automated analyses 1/2
connect.biorxiv.org/news/2025/01...
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Priya Ramakrishna
9 months ago
Most plants can`t handle salt, but have evolved strategies to survive in it. How do they do it?🌱🧪 Excited to share some of our first insights using a new cryoelemental imaging technique to answer these questions at the subcellular scale!(1/8)
www.nature.com/articles/s41...
@nature.com
#PlantScience
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Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation - Nature
This study demonstrates that cryo nanoscale secondary ion mass spectrometry (CryoNanoSIMS) enables direct multi-elemental imaging at subcellular resolution of macro- and micronutrients or trace elemen...
https://www.nature.com/articles/s41586-024-08403-y
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woj_wie
Lorenz Lamm
9 months ago
Looking forward to talking about MemBrain-seg tomorrow and giving a sneak peek into other MemBrain v2 functionalities 👀🚀
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woj_wie
Max Planck Institute of Biophysics
9 months ago
At the intersection of art and science 🎨🧪 some masterpieces reveal fascinating lessons in
#biophysics
! Join Prof. Werner Kühlbrandt on a unique tour of the Städel Museum, where he explores mirror symmetry, cellular energy, and more. Read more:
www.biophys.mpg.de/2840124/art-...
🔗
#ArtMeetsScience
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woj_wie
John Rubinstein
9 months ago
We are hosting a cryo-EM grid nanofabrication workshop on 28 April 2025, as part of the Canadian Cryo-EM Nexus (
ccemn.ca
). Learn how to make holey gold or holey carbon grids in your own laboratory! Space is limited, so please apply at
web.cvent.com/event/ee88ac...
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woj_wie
Philippe Van der Stappen
9 months ago
More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint:
www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal:
cryoetdataportal.czscience.com
#TeamTomo
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Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii
In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production...
https://www.biorxiv.org/content/10.1101/2024.12.28.630444v1
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woj_wie
Ben Barad
10 months ago
Tomorrow(!) morning at 9AM pacific, I am giving a webinar for EMS discussing the surface morphometrics pipeline and our approach to contextual structural biology
#teamtomo
. Free to attend, hope to see you all there!
us06web.zoom.us/webinar/regi...
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woj_wie
Caitie McCafferty
10 months ago
I am excited to share some of my PhD work on the motile cilia interactome revealed by XL/MS with
@jbwallingford.bsky.social
@edwardmarcotte.bsky.social
Ophelia Papoulas, Chanjae Lee, David Taylor, and
@builab.bsky.social
www.cell.com/developmenta...
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woj_wie
sofie.rec
10 months ago
Check out
@wojwie.bsky.social
and
@lorenzlamm.bsky.social
work on the thylakoid membranes in spinach! Wojciech stared to long at the segmentations and started seeing Van Goghs in it, so we made his fever dream a reality. More work from
#teamthylakoid
to come under the starry night skies of Basel!
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woj_wie
Matt Johnson
10 months ago
🥳New cryo tomography Preprint ☀️🌱🔬Molecular architecture of thylakoid membranes within intact spinach chloroplasts.
doi.org/10.1101/2024.1…
Proud to be part of this great work with
@cellarchlab.c
om
@wojwie.bsky.soci
al
@sheffieldpps.bsky.soci
al
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This one was delayed for a while… apologies to the musicians. Collab with Matt, Will and Lorna (over on Twitter). And a great dev effort from Lorenz
@lorenzlamm.bsky.social
. Ofc
@cellarchlab.com
team. Comment if you want to help.
#teamthylakoid
www.biorxiv.org/content/10.1...
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Molecular architecture of thylakoid membranes within intact spinach chloroplasts
Thylakoid membranes coordinate the light reactions of photosynthesis across multiple scales, coupling the architecture of an elaborate membrane network to the spatial organization of individual protei...
https://www.biorxiv.org/content/10.1101/2024.11.24.625035v1
10 months ago
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woj_wie
Ben Engel
11 months ago
Starter pack with our community of researchers here at
@unibasel.bsky.social
🇨🇭, including some of us at the
@biozentrum.bsky.social
🧪
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woj_wie
Caitie McCafferty
11 months ago
@sofie-dot-rec.bsky.social
is here now too! Woo 🎉
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