Thank you @leukemiaresearch.bsky.social ! This discovery would not have been possible without your support!

Now online! Disparate leukemia mutations converge on nuclear phase-separated condensates

Scientists uncover nuclear droplets that link multiple #leukemias revealing new #therapeuticTarget. @gandhardatar.bsky.social @sciencyelmira.bsky.social @goodell-lab.bsky.social @superscijew.bsky.social et al @cellcellpress.bsky.social @bcmhouston.bsky.social www.bcm.edu/news/scienti...

Thrilled to share that my third year as a postdoc in the Goodell Lab at BCM begins with a paper published in @cellpress.bsky.social. Grateful for an opportunity to be a part of this incredible multidisciplinary project.

(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.

Patrick's paper is finally out, a label-free method to measure the composition of multicomponent biomolecular condensates!

BRISKET IN - 24 hours to the summer lab party!!! #RibackLab #CellularPhysicalChemistryGroup

How do chromatin remodelers use #IDRs to find TF binding partners? In our new Molecular Cell paper, we show that β-catenin is an adaptor that links SWI/SNF (cBAF) subunit ARID1A with binding partners via IDR-domain interactions. www.cell.com/molecular-ce...

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1... BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

Very excited to share our latest work in #MolCell showing NPM1 stabilizing the association of nucleolus associated domains (NADs) and recruiting G9a to establish their repressive chromatin states. www.sciencedirect.com/science/arti...

I am excited to share the latest paper from my lab where we leverage the selection bias of condensate-promoting oncofusions to uncover molecular rules governing condensate specificity and function. www.cell.com/cell/fulltex....

Now published! Big congrats to first author @gginell.bsky.social We are actively working improving/updating various aspects of FINCHES; don't hesitate to reach out if you run into issues, have questions. www.science.org/doi/10.1126/...

Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy: Molecular Cell www.cell.com/molecular-ce...

A Perspective by @stephanieaw.bsky.social and @fraserlab.com discusses ways macromolecules use conformational entropy to control binding, catalysis, and allostery www.nature.com/articles/s41...

New(ish) preprint! 📰What whole-cell patterns emerge from the arrangement, morphologies, and interactions of organelles in the 3D space of the cell? What would we see if we could gather ALL the whole-cell reconstruction data that's out there in one place? www.biorxiv.org/content/10.1... 🧵 1/11

Excited to share the @goodell-lab.bsky.social News&Views on 3 exciting studies by Vassiliou Lab, Chan Lab, and @trowbridgelab.bsky.social in @nature.com and @natcomms.nature.com, showing that #DNMT3A mutant (major driver of Clonal Hematopoiesis) blood cells depend on mitochondrial respiration.

In lab’s #FIRSTPREPRINT, we present methods to measure nanometer-scale organization around & between specific proteins in condensates in live cells. We uncover unexpected heterogeneity for a liquid-like phase with local meshwork spanning 10-50nm, stemming from ribosome biogenesis in the nucleolus.

CALVADOS-RNA is now published doi.org/10.1021/acs.... This is a simple model for flexible RNA that complements and works with the CALVADOS protein model. Work led by Ikki Yasuda who visited us from Keio University. Try it yourself using our latest code for CALVADOS github.com/KULL-Centre/...

Cuts in NIH research grants will have a lasting negative impact on science and medicine in the U.S. Grants will continue to fund specific research costs, but essential infrastructure like labs, advanced equipment and support staff are crucial for groundbreaking work.

Our #CellLineOfTheWeek is a 3-for-1 and fluoresces fibrillarin, nucleophosmin, and dCas9-KRAB! 🦀🧫 Our cell lines & plasmids are available for just the cost of shipping to support discovery: www.allencell.org/cell-catalog... 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social

Great article (in which I am quoted) about biomolecular condensates, the cellular phenomena I work on in my day job! 🧪 www.scientificamerican.com/article/myst...

This is a textbook example (condensates are only just reaching textbooks!) of why condensates matter, and how they tend to work. Here these liquid-like blobs create a microenvironment in the nucleus of cancer cells within which chromatin sustains a conformation accessible to transcription...

Microscopy Nodes is now up on bioRxiv! 🚀 This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev). High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬 www.biorxiv.org/content/10.1...

Curious how biomolecular condensates can localize ions? Using NMR, we found that condensates selectively bind and localize both chaotropic anions and kosmotropic cations. This alters the condensate composition, interface potential and RNA duplex stability inside. By @irissmokers.bsky.social

mRNA display continues to astound. Fantastic collaboration with Alex Buell, led by the peerless @rasmusnorrild.bsky.social. ~100,000 peptides and their propensity to join the phase separation of a model disordered protein. doi.org/10.1101/2024...

Nice to see CALVADOS agrees with the nu (0.51) from their data fit with the MFF! www.nature.com/articles/s41...

Meet the CALVADOS RNA model Ikki Yasuda, Sören von Bülow & Giulio Tesei have parameterized a simple model for disordered RNA. Despite it's simplicity (no sequence, no base pairing) we find that it captures several phenomena that depend on the charge, stickiness and polymer properties of RNA 🧬🧶🧪

Culture of mouse Embryonic Stem Cells, stained with SPY555-DNA from @spirochrome.com and recorded on an iSIM scope from @visitech.bsky.social. With ultra-sensitive Hamamatsu ORCA-Quest and recent Large Field-of-View upgrade, we can detect mitosis in 3D, for almost an hour, with 1-min resolution.

🎉 Thrilled to share my lab's first PhD defense! Huge congrats to Gandhar Datar and co-advisor Peggy Goodell! 🎓🔬 Gandhar's work in biophysics and leukemia led to the discovery of a new phase-separated condensate that drives leukemias. Stay tuned for more insights!

🎨 In case anyone need… The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏! Check it out 👇 bioart.niaid.nih.gov

Fantastic preprint from Alistair Boettiger & Kevin Wang labs. Instead of checking contact (200 nm), community association (600 nm) reveals crucial insight into multi-enhancer regulation. Also, very flexible association rules & Pol II more informative than H3K27ac! www.biorxiv.org/content/10.1...

The ability to model both folded domains and disordered regions in condensates means that we can now begin to use CALVADOS simulations to ask how and why the condensate environment changes protein folding stability 🧪 doi.org/10.1002/pro....

Our deep learning approach for accurately predicting disordered protein conformational properties (radius of gyration, end-to-end distance etc.) from sequence is published! Predict properties for individual IDRs at metapredict.net or analyze entire proteomes in seconds at github.com/holehouse-la...

In case you missed my Condensate Colloquium @SPP2191 talk, I put it on YouTube today: ”How embryonic genes visit chromatin-associated condensates to become transcribed“ All unpublished, all there for you :-) youtu.be/e60FpY90KN8?...

A labor of love - our review on why IDRs matter for cellular function is live in Nat. Rev. Mol. Cell. Biol: www.nature.com/articles/s41... Working with Birthe Kraglund on this was such a pleasure, and we hope this review will be helpful to a broad audience!

Season 14 (!!!) of IDPSeminars starts again on Oct 19th with a cracking lineup of speakers! Sign up to receive more info via our website idpseminars.com NB: We moved away from the 1st Thursday of the month to accommodate some travel constraints. Please repost - all welcome 🧪🔬 #AcademicSky

A double bill with lots of new proteins Clustering-predicted structures at the scale of the known protein universe www.nature.com/articles/s41... & Uncovering new families and folds in the natural protein universe www.nature.com/articles/s41... 🧪🧬🖥️