Thank you to the @embl.org and everyone involved for another amazing course 🔬💚.

🔦 Welcome to the EMBL Course 'Advanced fluorescence imaging techniques' happening this week! Participants are getting a good mix of lectures on expansion microscopy and the image scanning microscope as well as hands-on practicals 🔬🥼 #EMBLFluorImaging

Great to support the #EMBLFluorImaging Course at @embl.org Heidelberg over the past week. Thanks to the EMBL team and group of scientists, instructors, and technology partners for another great course. #Microscopy #SuperResolution #LiveCellImaging #EMBL #Bioimaging

Great to be back at the Imaging Cell Dynamics conference in a sunny Catalunya ☀️🔬... Thank you to The Company of Biologists for organising another amazing conference. If you're in attendance and want to talk all things ISM and iSIM, please swing by for a chat... and grab a few chocs 🍫.... 🔬🦠💙

VT-iSIM is now installed at EMBL Heidelberg for the next two weeks of workshops + demos 🔬 Live-cell super-resolution imaging with: • ~2× resolution • large FoV • high-speed imaging • minimal phototoxicity Excited to see what samples people bring. #microscopy #superresolution #EMBL

And apparently the big hit was “das große schwarze Mikroskop!” (the big black microscope, I.e. the @visitech.bsky.social iSIM with it’s black atmosphere enclosure)

Live-cell imaging in action 🔬 Our US partner, BioVision, is supporting the Quantitative Imaging course at Cold Spring Harbor Laboratory, with our #VT-iSIM system, helping researchers get hands-on with advanced microscopy. 💙🔬💙🔬💙🔬 #microscopy #cellbiology

An extra goodie, where we I tried to see whether Pol II clusters (cyan) are placed in pockets of low chromatin content, connecting via low-chromatin channels to NPC-rich export patches. I suppose both is the case.

For #FluorescenceFriday, I am sharing a first glimpse at Mona Wellhäusser's @mofrawe.bsky.social new nuclei expansion microscopy. Stained are bulk protein, bulk DNA, and nuclear pore complexes (NPC). Expansion is supposed to be 16x, in all three dimensions. Imaged with @visitech.bsky.social iSIM.

😍😍😍

Our R&D team have been having fun with the #OpenStand from Prior. Both upright and inverted frames have been put through their paces 🔬 and are a very elegant implementation of #OpenPlatformMicroscopy 🔬💙🔬💙🔬💙.

Another day, another #VTiSIM install 😉, and another system to support the research community @crick.ac.uk. This time with FRAP and Opto-Genentics 🎯🔬. Thank you to the wonderful Crick Advanced Light Microscopy group (CALM). Happy Imaging 🦠🔬💙💙💙.

Calling all Live-Cell Imaging Microscopists in the Philly Area The VT-iSIM will be housed at The University of Pennsylvania from 19th to 30th January. If you want to drop in and try your samples, take a few cool pics, or just say hi, please get in touch via DM 😊🔬.

Always great to attend the Facility Manager Meeting, organised by the @royalmicrosoc.bsky.social. Even better when it's 5 minutes from home 😂😉🦠🔬🔬💙💙

VT-iSIM - Live-Cell, Super-Resolution Imaging for all.... Get in touch to learn more 🔬🧫💙💙💙

The VT-iSIM incorporates two independent µLens arrays, each with 3,025 precision lenses. Together, they generate an array of diffraction-limited illumination spots at the sample plane and deliver pixel re-assigned PSFs directly to the detector, with no need for post-processing.

The VT-iSIM features VTi's patented 2D-Array scanning architecture; enabling scan, de-scan, and re-scan of multiple points of illumination and emission, with just a single moving element. Each scan produces a complete image, delivering ultra-fast, full-frame capture rates of up to 500 Hz.

With instant SIM technology, the VT-iSIM delivers super-resolution images in real time, with no reconstruction or post-processing required. You get spatial resolution down to 100 nm, reduced photo-bleaching, and across a wide field of view. Faster. Clearer. Smarter imaging.

Due to our innovative 2-D array scanning architecture, large independent arrays of µLenses, and the instant nature of re-assignment, the VT-iSIM delivers class-leading performance in photo-bleaching when compared to standard array and non-array scanning confocals, @2x spatial and axial resolution.

Always a pleasure and never a chore to be at @mblscience.bsky.social Woods Hole for @omibs-mbl.bsky.social Another wonderfully organised course with excellent participants. Thanks as always to the fabulous organisers, faculty, TA's, course directors and the amazing vendor faculty family 💚🦠🔬

The VT-iSIMz from VisiTech International delivers cutting-edge, live-cell, super-resolution imaging in a fully integrated, compact, and cost-effective platform, making advanced live-cell, super-resolution imaging more accessible than ever before. visitech.co.uk#contact

The #VTiSIM is ready to rock at The Medical University of Graz. Thank you for all of the support from the Bioimaging Team in support of this demo and happy imaging 🔬🦠😊

Save the date for what promises to be an amazing imaging/cell biology meeting!

If your planning to visit #MMC next week, and would like to look at the #VTiSIM up close, please drop by the Evident Scientific booth (stand 309). Demo slots can also be booked by clicking on the following link: app.simplymeet.me/evident-mmc-...

Thank you to Ian Dobbie for organising a great workshop (in a very hot DC ☀️) on the commercialisation of imaging systems within bio-medical research, and for giving me the opportunity to discuss the story of the #VTiSIM. Great talks and great discussion.

DNA channel fluorescence intensity with overlaid object segmentation labels of chromosomes / cell nuclei in a zebrafish embryo. Acquired by Süheyla Eroglu-Kayikci on @visitech.bsky.social iSIM, labeled by student assistant Philip Kohle and rendered by PhD student Nils Friederich using @napari.org

Great to be back @embl.org for the Advanced fluorescence imaging techniques course 🔬🦠💚

VisiTech launches its new high-speed confocals! Capture ultrafast dynamics (1kHz) with our all-in-one solution, offering high temporal, axial, and spatial resolution across a large field of view, with minimal impact on your labs budget. Learn more: visitech.co.uk/#contact

Thrilled to announce that the registration for the EMBO workshop „Mitochondria in the Nervous System“ is now open. Join us from Sep 29th to Oct 2nd in beautiful Engelberg, Switzerland! meetings.embo.org/event/25-mit...

Always a pleasure to support the Quantative Imaging @QIatCSHL course at Cold Spring Harbor Laboratory @cshlnews.bsky.social with our #VTiSIM... 💚🦠🔬

Another week another #VTiSIM Install.... this time @vuamsterdam.bsky.social. Thanks to our partners #Evident and #Hamamatsu.... Happy Imaging 😊🦠🔬

Another week, another #VTiSIM demo. This week we're at The Hong Kong University of Science and Technology, with our partners Chinetek Scientific. The fun begins on Monday, and more updates to follow 🦠🔬💚

#FluorescenceFriday fixed cell data over the cell cycle in zebrafish embryo, Hoechst & IG against active enhancers and recruited Pol II. Recorded by @mofrawe.bsky.social on @visitech.bsky.social iSIM, prepared during Masters practical, credit also to @yuzhibao.bsky.social & TA Alicia Günthel

The crime was perpetrated thusly: a 15 µm z-stack of (n1)StayGold(c3)-CAAX in a U2OS cell imaged, with 200 nm spacing, every 30 seconds on a Visitech iSIM. (scale bar = 5 µm) Shown below with depth-colour coding:

I am very proud to advertise our latest publication, "Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers," in Adv. Materials: advanced.onlinelibrary.wiley.com/doi/full/10..... We propose to use T4 bacteriophages for calibrating 3D super-res fluorescence microscopy. 🧵

At KCL I could quickly test the iSIM (http://www.visitech.co.uk/vt-isim.html) from the @NikonInst Center - quite nice for a Z-stack aquired in ~10 seconds

Another iSIM upgrade, this time at ETH Zurich. We've added our latest flat-field & full-field technology, as well as a second camera, FRAP and Cellsens (with our partners at Evident)..... Happy Imaging 🦠🔬

We're all ready to go for the first iSIM demo at The Medical University of Vienna 🦠🔬😊

As is traditional with iSIM @visitech.bsky.social, we see things we never saw before. The first noisy image from a faint sample- live ND7/23 cells transfected with NMDA receptors tagged with RFP & GFP. But we never saw the membrane before...highly satisfying. More to come! #FluorescenceFriday

Trying out inverted 3-color lookup tables in FIJI today. Here is recruited RNA polymerase II (red), elongating RNA polymerase II (black-ish), and DNA (blue). Fixed zebrafish embryo nucleus recorded with iSIM @visitech.bsky.social Any suggestions for color combinations for three-channel composite?

New iSIM is really taking shape. Thanks to @visitech.bsky.social @humboldtuni.bsky.social @dfgpublic.bsky.social #fluorescence #microscopy #biophysics #cellbiology

We're strong proponents of re-purposing old microscope frames, to give them a new lease of life, but this may be a step too far 😂...... Where's the c-mount? 🔬🦠💚

Done!! 😊🙌

Our new iSIM has arrived in Berlin! Excited for installation by @visitech.bsky.social next week. Thanks to colleagues at @humboldtuni.bsky.social and @dfgpublic.bsky.social for supporting this application. And million thanks to @lennarthilbert.bsky.social for the crademos! #biophysics #microscopy

The VT-iSIM has come to Poland 😁. Now joining the lab of Adam Klosin at the Nencki Institute working alongside our partners at Evident... Happy imaging 🪱🔬

Culture of mouse Embryonic Stem Cells, stained with SPY555-DNA from @spirochrome.com and recorded on an iSIM scope from @visitech.bsky.social. With ultra-sensitive Hamamatsu ORCA-Quest and recent Large Field-of-View upgrade, we can detect mitosis in 3D, for almost an hour, with 1-min resolution.

We finally ran out of the burning building and could once again see the Bluesky (pun intended 🙃). To anyone who followed us on the platform that shall not be named, please follow again and we'll follow right back at ya'. Is it just me, or do I feel as though I'm home again ❤️🩷🧡💛💚💙🔬🔬🔬