Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes. @nfrk92.bsky.social @narjournal.bsky.social academic.oup.com/nar/article/...

🚨New paper out! #MicroSky Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival. We expanded its genetic toolbox: 🧬promoters to fine-tune expression 🧬IPTG-inducible system 🧬CRISPRi for rapid knockdown bit.ly/46GUn2c 1/4

I wrote a Preview in @cp-cellhostmicrobe.bsky.social ! A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio. Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters! www.cell.com/cell-host-mi...

Check out @julielebris.bsky.social’s thread on our latest manuscript describing phenotypic heterogeneity in capsule production in Klebsiella & Acinetobacter @klebclub.bsky.social This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social #microsky #phagesky

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Happy to see our paper out! Functional assessment of anti-phage defence systems in their native clinical E. coli host royalsocietypublishing.org/doi/10.1098/... Thread in previous post 👇

#ResultatScientifique🔎| Des scientifiques ont révélé comment le plasmide pOXA-48 rend les bactéries ultra-résistantes aux antibiotiques 🦠 🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social ✍️ @sbigot.bsky.social 📕 @natcomms.nature.com | buff.ly/u3Cy4Db

At last ! A tool specifically for phage-plasmid hunters. Check tyPPing by @karinailchenko.bsky.social and @eugenpfeifer.bsky.social

PhD opportunity, please share: We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA. All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...

Cool preprint by Mathieu Berge @polardlab.bsky.social showing that the Spr1630 toxin (RipA) toxin targets the replication sliding-clamp (DnaN) and that this induces competence in Streptococcus pneumoniae. www.biorxiv.org/content/10.1...

Have you ever wondered what increasing environmental stress will do to microbial communities? In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5) www.biorxiv.org/content/10.1...

🚨🚨New paper out in @natcomms.nature.com!! Come for the first large-scale analysis of plasmid copy number across species, stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬 👉 www.nature.com/articles/s41...

New paper out!

Our paper “Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli” is now online in @natcomms.nature.com ✨ 👉 www.nature.com/articles/s41... @cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social

Happy to finally share this! Read here about the best strategy the the persistence of low-copy plasmids. @mariosanter.bsky.social @iwangtoknow.bsky.social doi.org/10.1038/s414...

Our latest paper explores the factors shaping plasmid-bacteria associations and highlights why studying plasmid-host interactions of clinical relevance is key for understanding the evolution and spread of AMR. Find out more 👉 plasmidlab.es/publications/ #AntibioticResistance

We wrote 2 📕 chapters with tips & tricks to identify protein-protein interactions in bacteria 🦠 1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10.... 2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10..... Enjoy #MicroSky 🧪

Excited to share our newly book chapter on using #miniTurbo proximity labeling in #bacteria We wrote it as a hands-on guide for anyone looking to get started with this approach. Congrats to @coralietesseur.bsky.social and Ophélie Remy 👏 @lalouxlab.bsky.social link.springer.com/protocol/10....

We have an open engineer position to work on type IX secretion (T9SS), under the supervision of @doan_thierry (collab with HP Fierobe & A. Tolonen). 1-year contract, renewable up to 3.5 years. Please apply here or forward to anyone potentially interested: emploi.cnrs.fr/Offres/CDD/U...

You thought all OMPs were trimeric? 🧐 Not in our predator B. bacteriovorus! 🦠 Excited to have contributed to this great collab with the Lovering lab on this intriguing protein. @lalouxlab.bsky.social 👉 Paper here! www.nature.com/articles/s41... #BacterialPredation #Bdellovibrio #StructuralBiology

Finally published! Many thanks to the reviewers and editor whose comments & suggestions improved this work significantly more than the first preprint version!!! www.pnas.org/doi/10.1073/...

Nous recrutons un IE en bioinformatique pour faire de l'analyse évolutive de séquences biologiques (CDD de 22 mois) dans le cadre d'un projet ANR. L'offre est disponible ici : emploi.cnrs.fr/Gestion/Offr... N'hésitez pas à partager ! Merci ! 🙏 Me contacter pour toute info supplémentaire.

Fellow bacterial cell biologists, message me if you use @thermofishersci.bsky.social Gene Frames for microscopy! Thermo are planning to discontinue these in September but have indicated they will look into this further. If I make list of labs using them Thermo might not kill the product Please RT🔬🦠

Résistance aux antibiotiques, pour en savoir plus sur le paradoxe des biofilms ⤵️ 🤝 @sbigot.bsky.social @knutdrescher.bsky.social @cnrs-rhoneauvergne.bsky.social‬ @mmsb-lyon.bsky.social‬ 👉 Lire l'article dans ‪@pnas.org‬ buff.ly/IWjV6LV buff.ly/Ok2lZU6

New tenure track assistant professor position in molecular microbiology in our department @dmf-unil.bsky.social @unil.bsky.social! We are casting a wide net for an experimental molecular microbiologist. Apply here: wwwfbm.unil.ch/releve/appli...

Marie Curie fellowship, post-doc hosting oppotunity in my lab. MSCA Post-doctoral Fellowship at IBPC Paris on RNA modifications in bacteria (FRANCE) 🦠💫 euraxess.ec.europa.eu/jobs/hosting...

1/6 Excited to share our review 🚀 🧬 CRISPR–Cas therapies targeting bacteria! #MicroSky

New opinion paper from the lab! www.cell.com/trends/micro...

Le MMSB propose une mobilité NOEMI Rejoignez l'équipe "Ecologie et Évolution Moléculaire des Phages" qui travaille sur les éléments génétiques mobiles et l'immunité bactérienne. Profil Génétique bactérienne, Écologie microbienne et Evolution expérimentale 🤝Contactez-nous pour rejoindre notre unité!

I *finally* get to share our work on plasmid competition– super proud to now have this on bioRxiv: CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin-antitoxin activity when horizontally transferred www.biorxiv.org/content/10.1... 1/6 👇

cool preprint from a few months back: www.biorxiv.org/content/10.1... Really intriguing IS strategy to become fixed in plasmids, where the IS cuts up any plasmid that doesn't contain the insertion sequence

Pleased to share a new preprint - a great collaboration with @jessicaandreani.bsky.social and the Guerois lab at I2BC, and the Radicella lab at the CEA. Using stuctural modelling, we reveal & validate a 3 protein complex involved in DNA import during transformation. www.biorxiv.org/content/10.1...

At least 10% of the V. cholerae integron cassettes have novel antiphage activities (see below). Considering that there are 25,000 distinct cassettes of unknown function, integrons constitute an extraordinary and almost inexhaustible reservoir of antiphage functions!

Our paper on how integrons are biobanks of novel minimal defense systems is now out www.science.org/doi/10.1126/... Two main conclusions on this excellent work led by @eloilittner.bsky.social @baptistedarracq.bsky.social 1/n

🧪 Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉 Short 🧵 www.science.org/doi/10.1126/...

Le LISM (lism.imm.cnrs.fr) propose une mobilité en NOEMI pour travailler sur des assemblages de l'enveloppe bactérienne. Profil microbiologie, biologie moléculaire, avec une envie de faire de la biochimie, de la microscopie ou de la biologie cellulaire ! Rejoignez une unité dynamique !!

Our latest publication has just been released. As I am no longer working on this project, I can share the strains with any team wishing to continue it. It was fun but impossible to work on everything. #pseudomonas #putida enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...

Check out our latest work from the lab. Resistance genes perform differently in aerobic and anaerobic conditions! Thanks to all people from the lab involved (especially Alberto) and to collaborators @sanmillan.bsky.social Rafael Canton and the unique Fernando Baquero (big check in my bucket list)

For those interested on the bacterial envelope and on secretion system: a 2-day symposium in Marseille ! Second edition of the Secretion Days !

New postdoc position in our lab (2 y+): evolutionary genomics of integrons and MGEs with focus on vibrio-phage interactions. Great environment @pasteur.fr for science, career building. Super collaborators @celineloot.bsky.social @amazeld.bsky.social @fredoleroux.bsky.social 3 weeks to apply!

Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬 www.science.org/doi/10.1126/...

New publication from our Lesterlin lab in collab with lab of @knutdrescher.bsky.social We determined the spatiotemporal dissemination of conjugative plasmids within biofilms and found that they spread only in specific structural regions. www.pnas.org/doi/10.1073/...

Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions! Collab with lab of @sbigot.bsky.social & Christian Lesterlin www.pnas.org/doi/10.1073/...

Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩 Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭! Please visit my lab's webpage for details about the position and how to formally apply.

New preprint with nice detective work by PhD student Khadija Hanga: gain of pOXA MDR plasmid is facilitated by chromosomal mutations affecting OmpF which act synergistically with the plasmid’s carbapenamase to give high level resistance. Led by @mbottery.bsky.social www.biorxiv.org/content/10.1...

Preprint: Conjugative transfer inhibition of IncA and IncC plasmids by pervasive SGI1-like elements via relaxosome assembly interference #plasmid #relaxase #bacteria #parasite #MGE #plasmidbiology www.biorxiv.org/content/10.1...

📖 New publication from the TacC team at MMSB! Congratulations to Nathan Fraikin et al.! @nfrk92.bsky.social @agathe-couturier.bsky.social

Huge shoutout to 3 amazing woman scientists at MMSB recently recognized with major research awards! 🌟 Dr Anja Böckmann–Impulscience® grant 🌟 Dr Mathilde Guzzo–FEBS Excellence Award 🌟 Dr Anne Chevallereau–FSER Dotation Brilliant science, strong leadership, powered by women!💪🔬#WomenInScience #CNRS

The study on the FIB replicon of plasmid F that I talked about at Plasmid Biology 2022 in Toulouse is eventually published in Nucleic Acids Research: academic.oup.com/nar/article-...

C'est officiel : les inscriptions à l'ETMM 2025 ouvrent le 🗓️ lundi 7 avril 🔗 lcb.cnrs.fr/ecole-themat... N'attendez pas, les places sont limitées !