Erwan Gueguen (@macerwan.bsky.social)

Harnessing Small RNAs as Synthetic Post-transcriptional Regulators in Bacteria Bacteria can respond to environmental changes by expressing small RNAs (sRNAs), which regulate mRNAs by complementary base-pairing. This regulatory mechanism allows bacteria to rapidly adapt their proteome. In recent years, sRNAs have gained attention as blueprints for synthetic regulators allowing control over user-defined targets. Multiple aspects need to be considered for efficient application of these versatile, on-demand, and easy-to-use tools. Advances in computational prediction and bioengineering concepts are the dawn of systematic synthetic sRNA biology. We provide an overview of sRNAs and alternative post-transcriptional regulators, highlight the requirements for successful regulation, and provide guidelines for design, construction, and sRNA application. https://pubs.acs.org/doi/10.1021/acssynbio.5c00118