Nathan Fraikin
@nfrk92.bsky.social
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Post-doc at TacC team / CNRS / Lyon 1- Catching bacteria on tape right in the act !
reposted by
Nathan Fraikin
Géraldine Laloux
8 days ago
🚨New paper out!
#MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival. We expanded its genetic toolbox: 🧬promoters to fine-tune expression 🧬IPTG-inducible system 🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
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Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
https://academic.oup.com/microlife/article/doi/10.1093/femsml/uqaf021/8245127
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Nathan Fraikin
Agathe couturier
8 days ago
Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
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Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes
Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena
https://academic.oup.com/nar/article/53/17/gkaf898/8252029?utm_source=advanceaccess&utm_campaign=nar&utm_medium=email
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Nathan Fraikin
Knut Drescher
5 months ago
Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions! Collab with lab of
@sbigot.bsky.social
& Christian Lesterlin
www.pnas.org/doi/10.1073/...
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Biofilm architecture determines the dissemination of conjugative plasmids | PNAS
Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic res...
https://www.pnas.org/doi/10.1073/pnas.2417452122
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Our set of new prokaryote-optimized fluorescent proteins is now published !
#microbiology
#microsky
#microscopy
🧫🦠🔬
www.science.org/doi/10.1126/...
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A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging
Bright and photostable monomeric fluorescent proteins enable long-term time-lapse imaging in E. coli.
https://www.science.org/doi/10.1126/sciadv.ads6201
5 months ago
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Nathan Fraikin
MMSB-Lyon
5 months ago
MMSB - Molecular Microbiology and Structural Biochemistry
@mmsb-lyon.bsky.social
is now on Bluesky! 👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...
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Nathan Fraikin
Alvaro San Millan
8 months ago
New preprint from the lab!! Check it out!
add a skeleton here at some point
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Nathan Fraikin
Sarah Bigot
8 months ago
New preprint from our Lesterlin Lab! Yannick Baffert and
@nfrk92.bsky.social
, in collaboration with
@macerwan.bsky.social
and
@val-meve.bsky.social
, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!
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Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli
Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...
https://www.biorxiv.org/content/10.1101/2025.01.23.634512v1
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Nathan Fraikin
Steve Diggle
almost 2 years ago
RIP Pierre. You were one of the best. Thank you for all you did for our field but more importantly, for me and all the people you helped. I will miss you. ❤️
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