We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. www.biorxiv.org/content/10.1...

Last week, Washington University made the perhaps questionable decision to award me tenure.

A single brain infusion of miRNA targeting CAG repeats slows the progression of Huntington's disease by 75% in patients. This is something that I never thought I would see in my lifetime. www.uniqure.com/investors-me...

🚀 Our new paper is out @natmethods.nature.com! Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells. 🔗 www.nature.com/articles/s41... 🧬 www.addgene.org/John_Ngo/

A tremendous honor! Thrilled & humbled to receive 2025 Keio Medical Science Prize for launching LLPS #phaseseparation field (= #softmatter + #cellbio) w collaborators esp @HymanLab. & Congrats to Akiko Iwasaki @virusesimmunity.bsky.social. www.princeton.edu/news/2025/09... #KeioMedicalSciencePrize

these people in my lab are amazing

🎉 Congratulations to HHMI Investigators Clifford Brangwynne and Akiko Iwasaki, the two winners of the 30th annual Keio Medical Science Prize. This international prize, awarded by Keio University in Tokyo, honors scientists who have made major contributions to the fields of medical or life sciences.

🎉 JUST ANNOUNCED: @haozhulab.bsky.social named project co-leader for UTSW liver cancer SPORE. His project will study how to prevent hepatocellular carcinoma — which accounts for >85% of liver cancer cases across the nation. Read more ⬇️ cri.utsw.edu/zhu-named-co... #relentlessdiscovery 🧪

🔬 APPLY NOW: Seeking faculty scientists in #stemcells #TissueRegeneration #metabolism & #cancerbiology. You ready for #relentlessdiscovery at our collaborative Institute? Learn more at cri.utsw.edu/careers & apply before Oct. 15. 🤜💥🤛 #jobs #faculty #facultyjobs #ScienceJobs 🧪

I lost some faith in academic science over the years. But selection of McKnight for a Lasker restores my idealism. Days later, I'm still smiling. To see my scientific hero recognized is quite special. Sure, confused as to why "this" and not the many "thats". But clubs and committees, man.

Steven McKnight was driving to Montana when he received the call about winning a #LaskerAward. "I was flabbergasted. I probably should have pulled over," he told me. That and more of the best in @science.org and science in this edition of #ScienceAdviser: www.science.org/content/arti... 🧪

The discoveries of D. Görlich & S. McKnight exposed the structures and functions of low-complexity domains within protein sequences, revealing new principles of intracellular transport and organization. 🧪 #LaskerAward #Lasker2025 #phaseseparation @mpi-nat.bsky.social www.youtube.com/watch?v=962P...

House FY 2026 Proposal keeps NIH funding at $48 billion, which maintains NIH’s budget roughly at the FY 2025. NIH is protected compared to other areas that face sharper reductions. appropriations.house.gov/sites/evo-su...

Proximity-specific ribosome profiling using LOV-BirA reveals two distinct strategies for mitochondrially-localized translation: one for long coding sequences and one for short. It was a pleasure to contribute to this beautiful work from Jonathan Weissman @weissmanlab.bsky.social‬ and Jingchuan Luo

Katz Early Stage Investigator Research Project Grant is back. Share with your ESI colleagues! grants.nih.gov/grants/guide...

Cryo grid optimization is not science. It’s not art either. It’s nihilism. Ugh. Guess I should add some pickle juice to my protein.

Great department! Most departments at UT Southwestern are actively or will soon be actively recruiting new PIs. Please consider joining us!

Fiedler does it again! Discovery of protein oligophosphorylation: www.nature.com/articles/s41...

I’m thrilled to share our preprint that uses deep learning to interrogate structure-function relationships in condensates! biorxiv.org/content/10.1... In here, we ask: can AI read condensate biology from pictures alone? Turns out yes... see what we discover below! 🤖🧬🖼️ (1/10)

Our squid primary cell isolation protocol is now out in @cp-starprotocols.bsky.social⭐️🦑🧫This protocol enables molecular studies of cephalopod biology, aging, and basic cell physiology. Thanks to @brangwynnelab.bsky.social Hailey Tanner and Josh Rosenthal! Check it out here tinyurl.com/3knvkhyh 🤩

Faculty search announcement 2025: Tenure-Track Assistant Professor in Microbiology at UT Southwestern. We seek candidates working in microbial pathogenesis (some preference may be given to bacterial pathogens). Come join our community! (1/5) www.utsouthwestern.edu/departments/...

"The Hsp40 co-chaperone DNAJC7 modifies polyglutamine but not polyglycine aggregation" www.biorxiv.org/content/10.1...

Interesting backstory…

Human DNAJC7 knockouts get ALS: link.springer.com/article/10.1...

Thrilled and deeply honored to be named a 2025 Pew Biomedical Scholar! Huge congrats to my friend and colleague @cri-utsw.bsky.social Javi, who’s also joining the Pew family as a Pew-Stewart Scholar. CRI is on fire—let’s go make some bold, groundbreaking science happen! 🔥🧪✨

🔥12 years in the making!🔥 So excited our paper is finally out in Cell Reports! Great collaboration with Dr. Robert Huber @ Trent U, funded by NSERC and CIHR. The evolutionarily conserved PRP4K-CHMP4B/vps32 splicing circuit regulates autophagy: www.cell.com/cell-reports...

this is a pretty incredible case study. congrats to the patient, Damien, @ndosenbach.bsky.social, and the dozens of others who contributed to this important clinical research.

Texas is fighting to #ENDALZ at the ballot box 🗳️, and the Association is leading the charge to drive change. Earlier this year, the state legislature established the Dementia Prevention and Research Institute of Texas (DPRIT). Now it’s up to the voters to decide whether or not to fund it.

Excited to announce our paper describing how allelic variants in ribosome biogenesis factors cause neurodevelopmental disorders. Congratulations to Chunyang Ni, Yudong Wei and Barbara Vona, our collaborators Jun Wu, Reza Maroofian, and Leqian Yu, and all the other authors from across the globe.

Happy to share the latest work from the lab, led by @mudgal17.bsky.social‬, in collaboration with the Weis lab @ethzurich.bsky.social. How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)

😡 The amount of bureaucratic barriers being thrown up for me to host an URM student from an urban high school and pay her a small stipend so she can afford to take this opportunity is making me absolutely stabby. Barriers to participation in STEM are real and undiminished.

The nuclear lamina strengthens the nucleus and organizes the genome. So what happens when you acutely degrade it in living cells? Not what we thought! (1/n) www.molbiolcell.org/doi/10.1091/...

Excited to announce that our collaboration with @briandaniels.bsky.social has been published today! So happy for everyone involved, but especially me and Marissa 😆. Check it out if you're interested in #neuroinflammation and the #BBB www.science.org/doi/10.1126/...

You may die, but sialophosphoproteins will live forever. www.nature.com/articles/s41...

I'm thrilled to announce my next career step-- I’m joining Genentech as a Principal Scientist & Lab Head in Discovery Oncology! I’ll be hunting new ways to target cancers using my background in disordered nuclear proteins.

If heterochromatin is really a liquid-like condensate, why is it not spherical? We investigated whether mechanical interactions between a condensate and a fiber network can explain the variety of morphologies seen in phase-separated nuclear compartments www.biorxiv.org/content/10.1...

This is an awesome story. Congrats Jun and Daniel!

Excited to share our new study in Cell—congrats to Daniel, co-authors, and our collaborators in the @peterlylab.bsky.social What began as pure curiosity led to a tool for modulating mitochondrial abundance in vitro and in vivo. A new direction for the Wu lab begins! www.cell.com/cell/fulltex...

It’s a tough time for science so it feels odd to mention positives, but the Wert lab has had some amazing positive funding news come through and we are seeking talented and motivated postdocs! Please share! labs.utsouthwestern.edu/wert-lab

www.biorxiv.org/content/10.1... from Andrew Belmont lab

This has a number of interesting observations regarding oncogenic fusion proteins in nuclear envelope and nuclear body organization. While I’ve largely moved on from phase separation, I like this. Congrats, Josh!

So far, no organelle we've seen comes close to the replication organelles from langatvirus infections: www.biorxiv.org/content/10.1...

I am excited to share the latest paper from my lab where we leverage the selection bias of condensate-promoting oncofusions to uncover molecular rules governing condensate specificity and function. www.cell.com/cell/fulltex....

so sick of my favorite journal, CHEST, rejecting my PERFECT lumbar puncture manuscripts! about time Johnny law cracks down on their ABSOLUTELY UNFAIR "peer" review!

Yay, Regina!

Happy to share our new study that was just published in Neuron! www.cell.com/neuron/fullt...

Goddamnit. Phase separation is back. In space form. 😡https://www.pnas.org/doi/10.1073/pnas.2403981121

Texas knows when to stick the knife in.