Quantification and analysis of multiplexed fluorescence in situ hybridization data using open-source tools #protocol #starprotocols #cellpress

I am pleased to serve as one of the Guest Editors for the upcoming @cp-starprotocols.bsky.social focused issue on “Protocols in Metabolomics and Lipidomics Research.” We welcome detailed and comprehensive protocols that provide broad utility to the community. #Metabolomics #Lipidomics #MS #NMR

Maddie, Andoni and Agustin, the three PhD students in my lab just had their paper accepted in @cp-starprotocols.bsky.social!! 🥳 They describe a protocol for quantifying the horizontal and vertical distribution of junctional proteins in confluent, fixed epithelial cells. doi.org/10.1016/j.xp...

🚀 Thrilled to kick off my Bluesky feed with some exciting science! Are you (or your colleagues) working with ex vivo tissue culture or tissue infection models? 🧫🦠 We just published a new protocol in @cp-starprotocols.bsky.social 🎉 star-protocols.cell.com/protocols/4475

Protocol to culture cells on genipin-mixed collagen gels with different stiffnesses #protocol #starprotocols #cellpress

Protocol for designing and developing a population-based individualized intervention planning assistant for overweight and obesity #protocol #starprotocols #cellpress

Protocol to predict the origin of cancer of unknown primary from gene expression data using BPformer #protocol #starprotocols #cellpress

Protocol for experimental infections with viruses in mosquitoes using a glass artificial feeder #protocol #starprotocols #cellpress

Protocol for reproducible EZ clearing and labeling, including optimized steps and quantified fluorescence retention on mouse tissue #protocol #starprotocols #cellpress

Protocol for contactless and instantaneous line-current data exchange between MATLAB and a drone-deployable sensor on overhead transmission lines #protocol #starprotocols #cellpress

Protocol for trapping transient endogenous formaldehyde in live cells to visualize its signaling #protocol #starprotocols #cellpress

Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiation #protocol #starprotocols #cellpress

Protocol to detect dilution cycles in chemostat experiments and estimate growth rate slopes with linear modeling with R software chemostat_regression #protocol #starprotocols #cellpress

Protocol for immunofluorescence imaging of epitope-tagged protein in Arabidopsis shoot apical meristem #protocol #starprotocols #cellpress

Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain #protocol #starprotocols #cellpress

Protocol for simplified parallel perturbations using an abridged long non-coding RNA CRISPR library #protocol #starprotocols #cellpress

Protocol for integrating immunohistochemistry and H&E annotations with Xenium data at single-cell resolution #protocol #starprotocols #cellpress

Protocol for quantifying PARP inhibitor-induced changes in PARP1 dynamics and activity through live-cell imaging #protocol #starprotocols #cellpress

Protocol for selective DREADD-based chemogenetic inhibition of GABAergic amygdala neurons receiving hippocampal projections in rats #protocol #starprotocols #cellpress

Protocol to set up the CellMinerCDB pharmacogenomics analysis web application with custom cell line data #protocol #starprotocols #cellpress

Protocol to set up the immuno-microfluidic system for rapid measurement of salivary cortisol level #protocol #starprotocols #cellpress

Protocol for quantifying extracellular and intracellular ATP from macrophages upon inflammasome activation using a luciferin-luciferase technique #protocol #starprotocols #cellpress

Protocol to assess the cytotoxicity of autologous human skin immune cells against senescent fibroblasts #protocol #starprotocols #cellpress

Protocol for monitoring the growth of Solanum betaceum non-embryogenic callus using electrochemical impedance spectroscopy #protocol #starprotocols #cellpress

Protocol for Hi-C-based identification of chromosomal inversions in mosquitoes #protocol #starprotocols #cellpress

Protocol for single-base quantification of RNA m5C by pyrosequencing #protocol #starprotocols #cellpress

Protocol for isolating and characterizing extracellular vesicles by ultracentrifugation from bone marrow-derived macrophages #protocol #starprotocols #cellpress

Protocol for a human placental explant model to study acute responses to pathogens #protocol #starprotocols #cellpress

Protocol for contactless electric current sensing, processing, and storage using a drone-integrable sensor #protocol #starprotocols #cellpress

Protocol for iterative in vivo selection of highly metastatic ovarian cancer cell populations and metastasis modeling in mice #protocol #starprotocols #cellpress

Protocol for triggering plant gene silencing by viral delivery of short RNA #protocol #starprotocols #cellpress

Protocol to study in vivo circRNA interactions in the mouse cortex using an RNA pull-down approach #protocol #starprotocols #cellpress

In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models #protocol #starprotocols #cellpress

Protocol for quantifying amino acids in small volumes of human sweat samples #protocol #starprotocols #cellpress

Protocol for genomic surveillance of Plasmodium falciparum antigens using amplicon-based PacBio long-read sequencing #protocol #starprotocols #cellpress

Protocol for in vivo BONCAT labeling of nascent proteins in Xenopus laevis tadpole brains with high temporal precision for quantitative analysis #protocol #starprotocols #cellpress

Protocol for the reactive synthesis of double-A-layer MAX phase Ti2Bi2C in a sealed quartz ampule #protocol #starprotocols #cellpress

Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation #protocol #starprotocols #cellpress

Protocol for chromatin immunoprecipitation and hematovascular function assays with differentiated mouse embryonic stem cells #protocol #starprotocols #cellpress

Protocol for RNA modification analysis by UHPLC-QqQ MS #protocol #starprotocols #cellpress

Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells #protocol #starprotocols #cellpress

Protocol for generation of immortalized retinal, choroid, and brain endothelial cell lines #protocol #starprotocols #cellpress

Protocol for measuring context-dependent cost-benefit decision-making in humans using a web application #protocol #starprotocols #cellpress

Protocol for developing a melanoma brain metastasis mouse model for preclinical drug testing and brain tumor progression monitoring #protocol #starprotocols #cellpress

Protocol for renal artery embolization via caudal artery in rats #protocol #starprotocols #cellpress

Protocol for permanent gene repression by CRISPR-adenine base editing of promoter CCAAT motifs #protocol #starprotocols #cellpress

Protocol for single-cell spatial transcriptomic profiling of cultured cells and engineered tissues without embedding or sectioning #protocol #starprotocols #cellpress

Protocol for screening inhibitors of the fungal sphingolipid-metabolizing enzyme ceramide synthase #protocol #starprotocols #cellpress

Protocol for quantifying the horizontal and vertical distribution of junctional proteins in fixed epithelial cells #protocol #starprotocols #cellpress

Protocol for monitoring mRNA translation and degradation in human cell-free lysates #protocol #starprotocols #cellpress