Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging Naked mole rats get much older than closely related mamals of a similar size. cGAS mutations may have an important role in this. www.science.org/doi/... 1/9

Happy to share this review just out in Current Opinion in Immunology on innate immunity in the respiratory tract - also check out the other reviews in this collection on Intrinsic Immunity edited by John MacMicking #ImmunoSky #IDSky #pulmsky #MedSky www.sciencedirect.com/science/arti...

#COVID19 revealed the urgent need for better ways to screen for & rule out respiratory virus infection. We present a nasal biomarker screening strategy to prevent & manage outbreaks @eBiomedicine.bsky.social #IDSky #MedSky #lungs @yaleschoolofmed.bsky.social www.thelancet.com/journals/ebi...

ASSIGNMENT: COMMENT ON SCHEDULE F 1 week left to comment about Schedule F, potentially making NIH institute and center director essentially political appointees. www.regulations.gov/commenton/OP... 1/n

Nice coverage of our H5 mRNA work by Jon LaPook tonight on @cbseveningnews.bsky.social www.cbsnews.com/video/the-sc...

Are you a scientist and not sure what to do to help with all the awfulness all around us? Inspired by Vietnam era activism, @standupforscience.bsky.social is hosting a series of "teach ins," in which scientists share a bit about their area of expertise and why it's important with the public. 🧪🦑🌎

Check out this news story that @nature.com wrote describing our recent preprint where we tested our H5 mRNA vaccine in calves. The data look good and we are currently testing the vaccine in lactating cattle...more to come!

The Nile from the International Space Station:

Once in a while. Good morning Blue Sky

Spectacular view of 100,000 people attending the #HandsOff march in NYC

Thrilled to share our @elife.bsky.social paper "Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages" by 1st author Dr. @marisaegan.bsky.social (@cambupenn.bsky.social alum, now faculty @swarthmorecollege.bsky.social)! elifesciences.org/articles/90107 🧵(1/n)

Top F.D.A. Vaccine Official Resigns, Citing Kennedy’s ‘Misinformation and Lies’ Gift link: nyti.ms/3DTuvVr

We are seeking to hire a lab tech, this is an HHMI funded position, please share the job posting: hhmi.wd1.myworkdayjobs.com/en-US/Extern...

The difference between Accuracy & Precision.

In a science round table discussion yesterday, my NJ senator @kim.senate.gov suggested a strong argument for NIH and federal funding is global competitiveness. We US-based scientists should start weaving this into our narratives.

'Neutrophils expressed notably low levels of STING, whereas forced expression led to uncontrolled systemic inflammation. During development in the thymus, Tcells dynamically & epigenetically restricted STING expression through DNMT1–mediated transcriptional silencing' #Immunology

@cp-trendsimmuno.bsky.social Using structure-function information from IFN-γ-binding proteins and biased agonists to uncouple immunostimulatory and immunosuppressive activities www.cell.com/trends/immun...

Today I'm filled with admiration for my university's president, Chris Eisgruber. He's standing up for Columbia and for universities as a home for freedom of ideas, debate, and thought - free of government attack. www.theatlantic.com/ideas/archiv...

My privilege to team up with FASEB President-elect Eric Kelley today. We visited the W. Virginia and #Vermont offices together to explain how science research saves lives, creates jobs and keeps the USA competitive. #FASEBonTheHill #FundScience #NIH #MedSky #AcademicSky #UVMLarnerMed🧪

UCSF, like all UC campuses, announced an immediate hiring freeze today. UCSF is the second largest employer in the city. There will be wide ripple effects in the Bay Area and beyond, including the future of biotech and pharma in the area. 🧪

If there's one thing that domestic violence research has taught me, it's that abusers set up no-win scenarios. They punish you whether you cooperate with them or whether you push back. The only way out is to strengthen ties with people outside of the relationship who can create new, better options.

My dear friend’s niece, who in October developed sepsis when a Georgia hospital refused her a D&C after a miscarriage, will be taken off life support tomorrow. I don’t know the names of the countless others who have suffered and will suffered because of anti-abortion laws. But her name was Haley.

Please consider nominating deserving colleagues (including yourselves) who would be excellent candidates for five open positions on the Council, including Nominations for President-elect, Secretary, Treasurer, and 2 Councilors. Please share widely! @official-smbe.bsky.social

I’m a biomedical researcher who lost his wife to cancer. Finding cures is personal to me. What’s happening at the NIH is devastating. Here’s my editorial in my hometown newspaper. www.vindy.com/opinion/edit...

Thank you @vvsmider.bsky.social for sharing this story.

#WeekendRead! #InflammasomePower! @rauchlab.bsky.social &co show @jem.org that small intestinal tufts cells activate the NLRC4 inflammasome leading to PGD2-dependent IL-22 production by ILC3, protecting against Salmonella! rupress.org/jem/article/...

This 😭 via @economist.com

Looking for a research assistant post in London? 🤔 Interested in how anti-cytokine auto-antibodies are associated with immune dysfunction and disease during ageing? 🎯 Then do consider applying for this exciting post 👇 #ImmunoSky #IDSky @uclmedsci.bsky.social www.ucl.ac.uk/work-at-ucl/...

as I tell my Genetics class every year, this breakthrough is based on 70 years of NIH-funded, basic research, commercialized by biotech bsky.app/profile/need...

In my 25 years since high school I’ve seen 1. HIV/AIDS go from death sentence to manageable with drugs 2. Cystic fibrosis go from pretty much an extended death sentence to manageable with drugs 3. Sickle cell go from a horrible disease to (almost maybe) cured by gene therapy Simply incredible

Today @natmedicine.bsky.social published our study showing the older individuals born prior to 1968 have more antibodies that cross-react to #H5N1 relative to younger individuals. If H5N1 causes a pandemic, children will likely be the most susceptible. 1/2 www.nature.com/articles/s41...

West Texas measles outbreak spreads into Oklahoma www.nbcconnecticut.com/news/nationa...

White House Withdraws Nominee for C.D.C. Director Story by: @apoorvanyt.bsky.social and @sherylnyt.bsky.social Gift link: nyti.ms/3XQenKZ

1/ 📢 Register for the 2025 Henry Kunkel Society Meeting on "Genes & Immunity" and join us in Belgrade from April 10-12, 2025. www.henrykunkelsociety.org/meetings/ #HKS2025 #Immunology 🧬🔬

Join us at "Innovations in Single Cell Omics" in Berlin 5.12-13 (+ night science workshop!) www.isco-conference.eu Organizers: @stefaniegrosswendt.bsky.social, @simonhaas.bsky.social @leifludwig.bsky.social, Ashley Sanders, @larsplus.bsky.social, @elimereu.bsky.social, @alejofraticelli.bsky.social

Check out this list of awesome #virome tools and be sure to add yours! github.com/shandley/awe... #phagesky #microsky

Perception of viral infections and initiation of antiviral defense in rice @nature.com www.nature.com/articles/s41...

Identifying the genomic loci associated with brain aging and repurposing drugs targeted to their pathways science.org/doi/10.1126/...

The Trump administration has terminated $800 million in grants to Johns Hopkins University, spurring the nation’s top spender on research and development to plan layoffs and cancel health projects

There's a reason the U.S. is the world-leader in biomedical research and innovation. This has created jobs, boosted local economies, bolstered the GDP (+$2 for $1 invested), and kept us in the lead globally. This is now all under threat of collapsing. Foolish and shortsighted.

The general trend is crazy. Here is Twitter vs Bluesky volumes for the last week. On some days they are equal in volume.

Sharing from a follower: Federal cuts to science & tech are impacting jobs, small businesses, and future innovation. We are a group of alumni of AAAS’ Science and Technology Policy Fellowship tracking these real-world consequences with the IMPACTS Survey—and we need your voice.

What started as a brief chat with @buvansr.bsky.social and @vgelfand.bsky.social at the 2023 CellBio meeting @ascbiology.bsky.social has grown into our published collaborative work on single vimentin IF dynamics and organization in cells. Love when stuff like that happens.

I guess ~39 other NIH-funded projects focused on vaccine hesitancy got a similar email today... 🛟 medsky episky idsky 🧪 sociology health policy

'In rewilded mice, we observed B cells in circulation with increased signs of maturation, alongside heightened germinal center responses within secondary lymphoid organs. Rewilding also expanded B cells in the gut, which was accompanied by elevated systemic levels of IgG & IgM'

Rule #8: Present Support of Science as a Means to Meet National and Local Goals. www.aaas.org/news/top-ten...

One of the most powerful moments of the day came from Emily Whitehead who told the story of how she was the first pediatric patient to receive CAR T-cell therapy for her leukemia at age 5: “I stand up for science because science saved my life. And that’s a fact.” @standupforscience.bsky.social

Inspiring day for scientists in Washington DC #standupforscience2025 Thank you @standupforscience.bsky.social