The best way to avoid being scooped ? > Be original.

Some proteins are primarily regulated by one mechanism: RNA abundance, translation, or clearance. The regulation of most proteins is dominated by different regulatory mechanisms across cell types. Gratifyingly, this complex regulation defines simple rules ⬇️ www.biorxiv.org/content/10.1...

Multiplexed DIA enabled multimodal proteomics of ~ 10³ single cells, including quant of protein synthesis & degradation rates. Our technologies can increase the multiplexing along different dimensions (mass, time) and thus scale up throughput further to ~ 10⁴ - 10⁵ single cells. 🔽

We quantified mRNA abundance, translation, protein abundance, protein degradation and cell growth across thousands of single cells from a mammalian tissue. The results revealed 𝐜𝐨𝐦𝐩𝐥𝐞𝐱 regulation & 𝐬𝐢𝐦𝐩𝐥𝐞 organizing principles: www.biorxiv.org/content/10.1... 🧵

America’s cancer research system, which has helped save millions of lives, is under threat in one of its most productive moments. Trump Is Shutting Down the War On Cancer www.nytimes.com/2025/09/14/m...

What limits scientific creativity and originality ? This article thoughtfully discusses many intertwined challenges, including: Cultural Shift Daily Grind Less Is More Their arguments generally resonate, except for one: 🧵

What drives conceptual advances in biology ? One perspective is offered by this excerpt from a Cell article by Michael Brown, Joseph Goldstein and colleagues: Explosive advances in biological knowledge are often triggered by episodes of conceptual convergence. 🧵

A clear summary of our approach & progress towards scaling up (single-cell) proteomics.

Nature is taking a good step forward. All journals should publish all peer reviews. Here's why: the-scientist.com/opinion-we-m... blog.slavovlab.net/2023/04/15/p...

The American experiment looks fragile. It has been a force for good, inspiring hope and lifting many people. Let us find the strength to carry on this wonderful experiment.

Large DNA models can predict sequences for proteins from existing functional classes using many example sequences from these classes. In what way are these predictions novel ? This seems sampling 𝐰𝐢𝐭𝐡𝐢𝐧 the training distribution.

Join our team and help realize a bold vision.

A beautiful example of how spatiotemporal dynamics can enable multiplexed measurements.

an we understand biology from "infinite" amounts of perturbation data ? - Only if we measure the relevant molecules. Perturbations and data scale 𝐜𝐚𝐧𝐧𝐨𝐭 compensate for missing the relevant molecules. 🧵

All genes have the same function: - Template gene products. The products are diverse. Their functions are complex and incompletely characterized.

Contrasting cell count and biomass distributions by cell type: ◼️ Human cell size and count are nearly inversely related. More quantitatively, ... 🧵

This makes me wonder about the premiums we pay on other Sigma products. Take advantage of the web-only promotion !

Preprinting can facilitate the dissemination of research results at affordable cost. vis @asapbio.bsky.social asapbio.org/asapbio-resp...

We started the single-cell proteomics conference in 2018 with two primary objectives: 1. Build a community 2. Share ideas and technologies within & across research fields, especially fields that rarely interact. single-cell.net

A wonderful article. We need more search for & synthesis of principles of this type.

Can we all have a moment of sympathy for DNA — it has had a rouge week 🧬 First, proteins not predicted by genetic code 😮 www.biorxiv.org/content/10.1... Then, inheritance without genes through amyloid protein 🤯 www.nature.com/articles/s41... Biology is amazing!

Cell-type analysis is useful. Yet, much functional variation occurs 𝑤𝑖𝑡ℎ𝑖𝑛 𝑎 𝑐𝑒𝑙𝑙 𝑡𝑦𝑝𝑒. ◼️ In this example, protein abundance varies over 100 fold within a cell type. youtu.be/8nhjKSTqeOs?...

A key mechanism controlling mitosis is the precise timing of over 32,000 phosphorylation events. Below are some of the key regulators of mitosis.

Large AI models are reported to achieve high accuracy (AUROC) predicting pathogenic variants across the genome. A preprint reports that the predictions are based on splice variants. Using only this info (no sequences, no AI) achieves AUROC=0.944 across noncoding variants. 1/2

Exciting to see our work highlighted here!

This article suggests that TF binding is determined by the sum of multiple, overlapping binding sites. Thus, competition between paralogous TFs for shared high-affinity binding sites may be influences by flanking nucleotides from overlapping, lower-affinity binding sites. 🧵

Dictates to the media.

These findings add to the increasing evidence that cancer is not driven solely by DNA mutations.

Surprising estimates about the biomass distribution on Earth: ◼️Terrestrial biomass is ~ 100-fold higher than marine biomass ◼️ Plans are mainly terrestrial, whereas animals are mainly marine ◼️ Live stock biomass exceeds human biomass

Life uses exploratory dynamics. It's a simple & powerful approach: ◼️ Variation followed by selection 1/2

I am reading a research article, and the key results are shown in supplemental figures, even though the authors used fewer main figures than the journal allows. Strange.

A timeless model for writing a research summary. 1/2

Proteins self-organize to build large structures, 100,000-fold larger then the proteins. It's elegantly dynamic ! Proteins constantly self-assemble and disassemble to build skeletal structures in our cells, shaping and orchestrating life.

This summer we received numerous thoughtful and constructive reviews. I am glad more journals now publish peer reviews and look forward to their publication. Peer review remains highly imperfect, but ... 1/2 blog.slavovlab.net/2023/04/15/p...

We share our technologies to maximize the benefit for the community. PSMtags enable 9-plexDIA with better sequencing and multiplexing. PSMtags support sensitive proteomic analysis of over 1,000 samples / day. parallelsq.org/psmtags

This provocative article does not mince words. The authors argue that genome sequencing and scRNA-seq yield findings challenging the notion that cancer is a ‘genetic disease’: "... the cancer research community has abandoned deep thinking for deep sequencing" 1/n

Sensitive mass spec requires efficient ion utilization. ◼️Slice-PASEF increases this efficiency and thus the sensitivity of MS. We demonstrate its benefits by analyzing the proteomes of single lymphocytes, which reflect benefits observed in other @slavovlab.bsky.social projects using Slice-PASEF.

A gene may template many dozens of products. Its products can have diverse, even opposing functions. Ascribing the functions of the products to the gene is a simplistic approximation that has outlived its usefulness.

The past decade has been full of exciting ideas, datasets, and discoveries. I am proud of our alumni who now drive innovation in industry, lead their own research groups, and spread a culture of bold and rigorous research. Excited for what the next decade brings.

An essential component of a virtual cell model.

𝐌𝐚𝐣𝐨𝐫 𝐫𝐞𝐬𝐮𝐥𝐭𝐬 𝐚𝐫𝐞 𝐫𝐚𝐫𝐞𝐥𝐲 𝐛𝐨𝐫𝐧 𝐩𝐞𝐫𝐟𝐞𝐜𝐭. Here's a breakthrough story in the words of the lead innovator: 𝑇ℎ𝑒𝑦 𝑤𝑒𝑟𝑒 𝑝𝑟𝑒𝑡𝑡𝑦 𝑠𝑖𝑚𝑝𝑙𝑒 𝑑𝑎𝑡𝑎 𝑓𝑟𝑜𝑚 𝑢𝑛𝑟𝑒𝑙𝑖𝑎𝑏𝑙𝑒 𝑎𝑛𝑑 𝑢𝑛𝑠𝑡𝑎𝑏𝑙𝑒 𝑒𝑞𝑢𝑖𝑝𝑚𝑒𝑛𝑡, 𝑏𝑢𝑡 𝑡ℎ𝑒𝑦 𝑠ℎ𝑜𝑤𝑒𝑑 𝑜𝑓𝑓 𝑡ℎ𝑒 𝑢𝑛𝑚𝑖𝑠𝑡𝑎𝑘𝑎𝑏𝑙𝑦 ℎ𝑖𝑔ℎ 𝑟𝑒𝑠𝑜𝑙𝑣𝑖𝑛𝑔 𝑝𝑜𝑤𝑒𝑟 𝑜𝑓 𝑡ℎ𝑒 “𝑂𝑟𝑏𝑖𝑡𝑟𝑎𝑝” -- 𝐀𝐥𝐞𝐱𝐚𝐧𝐝𝐞𝐫 𝐌𝐚𝐤𝐚𝐫𝐨𝐯

I started a Substack blog discussing a favorite topic: 𝐖𝐡𝐢𝐜𝐡 𝐩𝐫𝐨𝐣𝐞𝐜𝐭𝐬 𝐝𝐞𝐬𝐞𝐫𝐯𝐞 𝐨𝐮𝐫 𝐥𝐢𝐦𝐢𝐭𝐞𝐝 𝐭𝐢𝐦𝐞 ? open.substack.com/pub/nikolais...

The distribution of ~ 2 trillion immune cells in the human body.

Our findings (now published in @narjournal.bsky.social‬) identify the first metazoan RNase MRP-specific protein subunits. We also define the RNA-targeting repertoire of this essential enzyme in mammalian cells. academic.oup.com/nar/article/...

The key molecules in immune responses are glycoproteins. Why isn't glycosylation studied more intensively? It should be. It's a challenging research area, but a good one for ambitious & audacious scientists.

Mitochondria have many functions beyond their well-established bioenergetic and biosynthetic functions. They integrate physiologic inputs and communicate signals that control cell function or fate as well as adaptation to stress.

Tools have long propelled discoveries: "𝑁𝑜𝑡ℎ𝑖𝑛𝑔 𝑡𝑒𝑛𝑑𝑠 𝑠𝑜 𝑚𝑢𝑐ℎ 𝑡𝑜 𝑡ℎ𝑒 𝑎𝑑𝑣𝑎𝑛𝑐𝑒𝑚𝑒𝑛𝑡 𝑜𝑓 𝑘𝑛𝑜𝑤𝑙𝑒𝑑𝑔𝑒 𝑎𝑠 𝑡ℎ𝑒 𝑎𝑝𝑝𝑙𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑎 𝑛𝑒𝑤 𝑖𝑛𝑠𝑡𝑟𝑢𝑚𝑒𝑛𝑡" Sir Humphry Davy Elements of Chemical Philosophy (1812)

The registration for SCP2026 is open ! Join us next July for the the 9th Single Cell Proteomics Conference. single-cell.net/proteomics/s...

Scientists should actively promote good research. 𝐖𝐞 𝐧𝐞𝐞𝐝 𝐭𝐨 𝐝𝐨 𝐢𝐭 𝐭𝐡𝐨𝐮𝐠𝐡𝐭𝐟𝐮𝐥𝐥𝐲, 𝐜𝐫𝐢𝐭𝐢𝐜𝐚𝐥𝐥𝐲 𝐚𝐧𝐝 𝐜𝐨𝐧𝐬𝐭𝐫𝐮𝐜𝐭𝐢𝐯𝐞𝐥𝐲. blog.slavovlab.net/2020/07/15/p...

If you measure and interpret RNA levels in single cells, read this: "𝑂𝑢𝑟 𝑟𝑒𝑠𝑢𝑙𝑡𝑠 𝑠𝑢𝑔𝑔𝑒𝑠𝑡 𝑡ℎ𝑎𝑡 𝑖𝑛𝑠𝑡𝑎𝑛𝑡𝑎𝑛𝑒𝑜𝑢𝑠 𝑚𝑅𝑁𝐴 𝑎𝑏𝑢𝑛𝑑𝑎𝑛𝑐𝑒 𝑜𝑓 𝑐𝑎𝑛𝑜𝑛𝑖𝑐𝑎𝑙 𝑚𝑎𝑟𝑘𝑒𝑟𝑠 𝑖𝑠 𝑡𝑒𝑛𝑢𝑜𝑢𝑠𝑙𝑦 𝑙𝑖𝑛𝑘𝑒𝑑 𝑡𝑜 𝑡ℎ𝑒 𝑐ℎ𝑜𝑛𝑑𝑟𝑜𝑔𝑒𝑛𝑖𝑐 𝑝ℎ𝑒𝑛𝑜𝑡𝑦𝑝𝑒 𝑎𝑡 𝑡ℎ𝑒 𝑠𝑖𝑛𝑔𝑙𝑒-𝑐𝑒𝑙𝑙 𝑙𝑒𝑣𝑒𝑙." 1/2