For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper? You got it: currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Who is looking for grad students for next year? I have a group of highly-motivated, aspiring scientists in paleontology, evolution, and ecology who would love some leads on programs.

MMseqs2-GPU sets new standards in single query search speed, allows near instant search of big databases, scales to multiple GPUs and is fast beyond VRAM. It enables ColabFold MSA generation in seconds and sub-second Foldseek search against AFDB50. 1/n 📄 www.nature.com/articles/s41... 💿 mmseqs.com

This fall I will have a blind student in my coding class for the first time. Do any other instructors or visually impaired coders have advice beyond making sure my book has useful alt-text for the images? #rstats #accessibility Class book: psyteachr.github.io/reprores-v5/

Hey Sciencers🧪, Someone suggested a solution to the problem when a loading control for a Western blot is so bright that it is certainly outside the linear range. They said just use way less primary antibody. This strikes me as wrong, but I dunno, maybe I'm wrong.

🚨Big news🚨 A Pocket Guide to Scientific Writing and Publishing is out🎉 This is the book I wish I’d had 20 years ago — short, practical, and designed to help researchers write & get their papers published I hope it helps many Please share with anyone who might benefit! 👉 mybook.to/ScienceGuide

Removing BA science options also hurts transfer students in some cases where not all courses are acceptedas equivalent major courses, and a lot of transfers are Community College transfers.

Merck is accepting applications for its 2026 Future Talent Program - these are internships and co-ops in a variety of areas including drug discovery, vaccines, clinical trials, biostatistics, epidemiology, HEOR, data science, regulatory affairs, and more #chemsky 🧪⚗️ jobs.merck.com/us/en/search...

Mushroom mayhem continues. Partial harvest of the shitakes so the others have room to grow.

First for me wordle ⬜⬜⬜⬜⬜ ⬜⬜⬜⬜⬜ 🟩🟩🟩🟩🟩

Is there a piece of writing on college's "hidden curriculum" (all the unspoken rules of college) that your students have appreciated? Can be short, long, whatever.

Wait till you guys hear about car drivers

The thing about an ebike is i bought one and now i am biking like 30 miles a week instead of zero miles a week on the regular bike i love but don't think about using every day.

Electric bikes are surprisingly great for mild cardio exercise, which is one of the best things we can do for health. Although ebikes are less exercise per mile than regular bikes, they tend to get ridden a lot more miles because they're so fun.

We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:

It's such a relief when an unexpected result gets replicated.

Another doom scroll break. Garden produce!

Let me break your doom scrolling with and important message. Fall mushroom season has officially started in my garden!! Chestnuts on logs, shiitakes on wood bricks and logs, snow oyster on logs and buckets, and we might have more lions mane.

I thought I'd try this technical molecular microbiology question here. #MicroSky Does anyone have experience with cloning 7-10 kbp fragments into a vector for Gram-positives? We are trying, but the vectors we generally use become quite unstable when we try to clone in a fragment of that size.

Yeah, what I'm guessing for us is scaling back probably = less flexibility and a more direct recruitment. Fewer pots of money and TA slots to solve problems feeds into it as well. Even now we are down to offering only two quarters of guaranteed support. Thanks for your response.

How do cells keep their #actin “highways” stable?🚦 Our paper @PLOS Genetics reveals surprising teamwork (and backup plans!) between tropomyosin isoforms in yeast. #imaging #cytoskeleton #celldivision This work was brilliantly led by @anubhavdhar.bsky.social @bagyasree.bsky.social t.co/RvUrzad5tL

OK--fluorescent protein friends. We want an antibody to TagRFP that works in immunofluorescence. Any suggestions? Re-post please

Applying to the NSF-GRFP (or another fellowship) on a tight deadline? We built a 7-week guide + timeline to get you from draft to submission. It’s not too late — you’ve got this! ✨ 🔗 cientificolatino.com/apply-in-7-weeks #NSFGRFP #GradSchool #Fellowship

While full solicitation isn’t up, looks like we have 2025 proposal deadlines for the GRFP: www.nsf.gov/funding/oppo...

Willing to join us @pasteur.fr for a PhD for a project on how interactions between mobile genetic elements shape bacterial adaptation? Subject to be tailored to candidates with keen interest in evolution, genomics, computational biology, microbiology. Check www.pasteur.fr/en/education...

Our latest preprint on the role of the Arp2/3 complex in mitotic entry is now available @biorxiv. This work was done by Dhanya Kalathil, a very talented postdoc in my lab. The story started in 2019 when we were investigating the role of branched actin in cytokinesis. 1/

Does anyone have #zebrafish LC3 reporter lines and would be willing to share them with us? #askzebrafish

I never thought watching microtubules break could be so interesting! I learned so much about mechanics and modeling in this work and am extremely grateful to Archie Geng for leading the way. Also huge thanks to François Nédélec for creating Cytosim and providing advice along the way.

🧪Excellent start for the day. I woke up pre-dawn with what seemed like an excellent idea for an experiment, sent myself a quick email so I wouldn't forget. Amazingly, after having coffee and reviewing my notebooks, it is still a good idea, and most importantly, we have not yet done the experiment.

Per Ljungdahl kicking off the 40th SMYTE meeting in beautiful Stockholm yesterday 😃 Programme looks brilliant, ready for a few days immersed in yeast transport 🤓

Phage folks, does anyone have suggestions for cheaper alternatives for low melt agarose for phage plaque assays? Much appreciated!

And you can do it even if you have literally bad hands—I spent years doing very successful bench work with a serious hand tremor Learn PCR (not a protocol, the actual techniques so you can troubleshoot and improve any protocol), plant your elbows, and go

🧵In collaboration with Caroline Puente-Lelievre, Ed Moody, Rosie Cater, and Tom Williams we just submitted a new paper describing the prokaryotic origins of the COMMD protein family. @cpuentelelievre.bsky.social‬, ‪@rosemaryjcater.bsky.social‬. @kmichie.bsky.social‬. 1/n

@mcduncanlab.bsky.social wrote a very nice piece about this. www.molbiolcell.org/doi/10.1091/...

Please re-post: If you know (or are!) somebody who might fancy doing a PhD (Oct 2026 start) in my group @oxfordbiochemistry.bsky.social, working on chromatin evolution in prokaryotes (or other things we're interested in), please have a look at www.bioch.ox.ac.uk/supervisors-...

Global Bioimaging are running a survey on Authorship and Acknowledgements for imaging core facility staff. If you work in a core, please take five minutes to fill out the survey and help us understand the good, the bad and the ugly around this topic. Thanks! Link: forms.gle/cKh6m4SskzZm...

Has anyone encountered DNA fragments that were hard to clone because “toxic” in E. coli? (Not because of expression). Any tools to detect that? #microsky

I keep getting angrier and angrier at journalists who perpetuated the entirely false narratives of “pace of innovation has slowed” and “why no more big discoveries”

Our latest is now available @jcellsci.bsky.social. The final research article from Bethany Campbell's thesis work. Endocytic patch dynamics are differentially regulated at distinct cell sites in fission yeast. doi.org/10.1242/jcs....

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1... Soon at @spirochrome.com Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

Whelp I give up. UMMS wants to delete all of our emails older than 2 years. This is mainly to comply with HIPAA, but I don't do health research. We can save emails we need to keep, but I'm not sorting through 10+ years of emails. The bulk export isn't working. So I'm letting them go poof.

Oh no I’m rapidly running out of the paperwork sections of grant writing ie reading the RFA carefully, revising my authentication plan…I’m going to have to start doing the thinking part soon.

Final year PhD students, I know you’re desperately trying to finish experiments/revisions/submissions/write a thesis & just survive - but please spare a few minutes to apply to your dream postdoc labs. It easily takes A YEAR from first contact, even if everything goes to plan on both sides.

🧪Ouch SIGMA bioreagent grade CHAPS-a detergent we use for our experiments-went up from $86/5g in 2023 to $71/1g or $275/5g. I wonder if this is tariffs or something else? Are people in other countries seeing massive price increases?

Faculty opening in Cornell Chemistry at the Assistant Professor level in the area of chemical biology, broadly defined! academicjobsonline.org/ajo/jobs/30338 #chemjobs

Was lucky to see this presented at the NYC cryoEM meeting earlier this year - super cool stuff!

encapsulate your target proteins for cryoEM structural determination within highly hydrophilic, structurally homogeneous, and stable protein shells 🤩 www.biorxiv.org/content/10.1...

Ghent implemented a circulation plan, in part, BECAUSE THEY DIDN'T HAVE MONEY FOR INFRASTRUCTURE. But the results were rapid: car traffic evaporated and cycling increased by 60%.

👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes