Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well! Peroxi_SPY can thus be combined with immunofluorescence labeling. A new article provides a detailed protocol: app.jove.com/t/69405/pero... Products link: spirochrome.com/products/?_c...

Happy new year from @membramics-lab.bsky.social members!

Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...

@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...

[PUBLICATION] The @membramics-lab.bsky.social team shows how keratin is involved in melanin genome photoprotection at the cell level. 👏🏼 Co-1st authors: Silvia Benito Martinez & @laurasalavessa.bsky.social 🔗 doi.org/10.1038/s415... 📰 Read more on INEM's LinkedIn, and website: tinyurl.com/yr9pntvb

This baby is finally out! Our study in @natcellbio.nature.com shows how keratinocytes use intermediate filaments and microtubules to control melanin positioning for optimal photoprotection. A real tour de force, together with Silvia Benito-Martínez and @delevoyecedric.bsky.social.

Reversible One-way Lipid Transfer at ER-Autophagosome Membrane Contact Sites via Atg2 www.biorxiv.org/content/10.1...

#LivingArchitectures We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay. www.musee-orsay.fr/fr/agenda/ev... Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night. -> Two shows on the 24th and 25th of January.

VAPB is a negative regulator of STING-mediated innate immune signaling | Science Advances www.science.org/doi/10.1126/...

Vimentin intermediate filaments support autophagosome biogenesis at ER-endosomes contact sites in response to starvation www.biorxiv.org/content/10.6...

Vimentin intermediate filaments support autophagosome biogenesis at ER-endosomes contact sites in response to starvation https://www.biorxiv.org/content/10.64898/2025.12.02.691851v1

My first postdoc preprint is out! Upon starvation, the Vimentin IFs network rapidly compacts (in <15 min!) to "cage" ER-Endosome membrane contact sites. This spatial confinement is crucial for autophagosome biogenesis. www.biorxiv.org/content/10.6... #CellBiology #Autophagy #Vimentin

onlinelibrary.wiley.com/doi/10.1111/... Check out our last review on endolysosomal plasticity in pigment organelles biology by @laurasalavessa.bsky.social @delevoyecedric.bsky.social in @biologyofthecell.bsky.social !

What a truly beautiful time at the European Intermediate Filament Meeting 2025 in Lyon! I was so glad to meet such a welcoming community. I'm honored to have won the Best Oral Communication Prize for my recent postdoc project on Vimentin and Autophagy! Huge thanks to the organizers!

New paper alert! I’m very excited to share our paper published in @embojournal.org! @pdc-lab.bsky.social @yaleneuro.bsky.social

Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧵(1/9) www.nature.com/articles/s41...

www.science.org/doi/10.1126/... Important study! Authors identify PDZD8 (renamed LYVAC) as an ER-anchored lipid transfer protein. In response to lysosomal osmotic stress, it provides lipids to support lysosome swelling. Unlike ATG2, LYVAC does not supply lipids to damaged lysosomes.

www.nature.com/articles/s41... EBV-expanded B cells home to the CNS where they attract T cells, mainly via CXCR3 expression, to trigger multiple sclerosis. Congrats to the authors !

New Online! Lysosomal membrane homeostasis and its importance in physiology and disease

Sad days. www.pnas.org/doi/full/10.... www.nature.com/articles/s41...

You know it's going to be good when it says 'ATG16L1-dependent'😜! Congrats on the great work @carlaalemany.bsky.social, @julianedagraca.bsky.social !!!

FINALLY OUT ! After a long journey (of PhD and publication process) my paper on the role of ER-endosomes contact sites in autophagosome formation is officially out 🥳🥳 www.cell.com/cell-reports...

🎉 Congratulations to @julianedagraca.bsky.social, recipient of the 2025 Beth Levine #ThesisPrize! Her outstanding research @membramics-lab.bsky.social on the role of ER/Endosome contact sites in the regulation of #autophagy captures the award's spirit of excellence and innovation. 👏

dear all! The Membrane Band (www.membraneband.com) will give a concert for the 30th Anniversary of the "Cell biology & Cancer Research Unit" (aka UMR144 for close friends) ‪of Curie Institute on Monday 23th fo June! @institutcurie.bsky.social see you there?

Our Immune Cell Biology collection emphasizes cellular dynamics, immune regulation, and disease mechanisms. Explore research on the mechanisms of dendritic cell regulation, T cell migration, neutrophil spreading and macrophage motility in tumor invasion, & more: rupress.org/jcb/collecti... #AAI2025

Quiescent cell re-entry is limited by macroautophagy-induced lysosomal damage: Cell www.cell.com/cell/fulltex...

The prizes for best presentations went to Léa Ripoll for her work on adenylate cyclase and dopamine signalling in neurons and Quentin Frenger for his work on ATG16L1 and B cell antigen receptor trafficking. Congratulations !!

I was very happy to present part of my thesis work at the 27th @clubexo-endo.bsky.social meeting and to win one of the prizes for the best oral communication! It was an excellent meeting packed with friendly people doing amazing science!

The V-ATPase/ATG16L1 axis drives membrane remodeling during epithelial morphogenesis www.biorxiv.org/content/10.1...

New lab preprint! Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy (Cryo-ExM). A fantastic collaboration with Dr. Benita Wolf. Congratulations to all authors, especially Florent Lemaitre, for leading this amazing work! www.biorxiv.org/content/10.1...

The microtubules of a B cell (magenta hot) relocalize upon recognition of an antigen (green) by the BCR.

At this level, it's witchcraft! Congrats !

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells. www.biorxiv.org/content/10.1...

We dedicated this paper to Michel Bornens who passed away 3 years ago. He would have enjoyed this movie so much!

"The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage" out now in Nature Cell Biology rdcu.be/ehbaL

My postdoc journey ... 2 labs, 2 cities, 2 animal models and 4 years of work, concentrated in 16 pages → my paper is out 🤩 @naturecomms.bsky.social #lysosomes #melanoma #metastasis #zebrafish #mouse #patientsamples #microscopy

www.nature.com/articles/s41... No peptide recognition by the TCR ? :O

This study from the labs of Wei Zou and Qiming Sun @ZJU_China uncovers Rab #GTPases as evolutionarily conserved signals mediating selective #autophagy, targeting damaged #mitochondria, #LipidDroplets, and #pathogens for degradation. rupress.org/jcb/article/... #mitophagy #lipophagy #xenophagy

Weekly "Non-canonical autophagy" report ! biomed.news/bims-nocaut/...

BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM www.biorxiv.org/content/10.1...

🏆 Congrats to @julianedagraca.bsky.social! Former PhD student at INEM, Juliane has been awarded the 2025 Thesis Prize of the @clubexo-endo.bsky.social for her work on ER-endosome contact sites & autophagy regulation. 👏 Well done, Juliane! @membramics-lab.bsky.social

Follow biomed.news/bims-nocaut for weekly updates on non-canonical autophagy papers.

Weekly "Non-canonical autophagy" discoveries (issue#143), thanks to @biomednews.bsky.social ! ⬇️⬇️⬇️ biomed.news/bims-nocaut/...

🚀Our latest work reveals how RAB5c orchestrates LC3-associated phagocytosis (LAP) to boost macrophage defense against A. fumigatus! 🦠🔬 🧵 Dive into the mechanisms linking endosomal traffic to innate immunity! #Immunology #Autophagy #Phagocytosis #Macrophage #Preprint doi.org/10.1101/2025...