How does the V-ATPase transmembrane core reach the phagosome during LAP (LC3 associated phagocytosis)? Live imaging shows V0 transfer occurs via contact between V0+ organelles and the phagosome membrane, a step controlled by RAB5c+ early endosomes. *STELLARIS Confocal Microscope #LeicaMicrosystems

A follow-up on one of the more unexpected findings from our recent @ScienceAdvances paper: RAB5c-dependent trafficking of the V-ATPase V0 sector to phagosomes. 🧵

There’s been lots of great science published in the past two weeks on #autophagy and #mTOR topics. Anyone with an interest in this area should look at these 👇🏼👇🏼👇🏼 biomed.news/bims-auttor/... biomed.news/bims-auttor/...

RAB5c coordinates early endosome-to-phagosome trafficking to drive LC3-associated phagocytosis in macrophages — and without it, fungal killing fails even when ROS production goes up. New paper out in #ScienceAdvances. Thread below 🧵

Autophagy-lysosome pathways are known targets of intracellular bacteria – Ivan Đikić @idikic.bsky.social et al find Legionella uses phosphoribosyl-ubiquitination via SidE to alter autophagic SNAREs & create a bacteria-containing vacuole unable to fuse with lysosomes www.embopress.org/doi/full/10....

Phosphoribosyl ubiquitination of SNARE proteins regulate autophagy in Legionella infection https://www.biorxiv.org/content/10.1101/2025.05.19.654886v1

#Autophagy and #mTOR papers and preprints from last week. As always a great selection, enjoy! 👌 biomed.news/bims-auttor/...

Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵 www.science.org/doi/10.1126/...

#Autophagy and #mTOR papers from last week, lots of relevant stuff as always, enjoy! biomed.news/bims-auttor/...

RAB5c controls the assembly of non-canonical autophagy machinery to promote phagosome maturation and microbicidal function of macrophages https://www.biorxiv.org/content/10.1101/2025.03.25.645097v1

Happy to share the latest work from the lab @crick.ac.uk. Outstanding work from Di Chen and Tony Fearns showing the link between Mtb membrane damage, calcium leakage and ATG8/LC3 lipidation in macrophages. Congratulations Di and Tony! www.science.org/doi/10.1126/...

Exciting news from the lab out today in Science - the structure of human PINK1 on a TOM-VDAC complex! @wehi-research.bsky.social

🚀Our latest work reveals how RAB5c orchestrates LC3-associated phagocytosis (LAP) to boost macrophage defense against A. fumigatus! 🦠🔬 🧵 Dive into the mechanisms linking endosomal traffic to innate immunity! #Immunology #Autophagy #Phagocytosis #Macrophage #Preprint doi.org/10.1101/2025...