I've written a review on what I think is an extremely exciting direction in cryo-EM: Cryo-EM of endogenous membrane proteins in their native lipid bilayer Open access in Quarterly Reviews of Biophysics: doi.org/10.1017/S003...

📣 The 2026 Midwest DNA Repair Symposium is coming to Kansas City (registration is open) 🗓 May 30–31, 2026 ✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators. Please RT & share with your labs! 🔗 sites.google.com/view/kumc-mi...

As construction of the room for our new Glacios CryoEM comes to an end - here's a quick look at the process. *Not pictured: lots of people to thank, many meetings, phone calls, challenges, delays, and teamwork. I learned a lot during this process - now I'm ready for installation and data collection!

🎉 Joint paper from the Freudenthal and @tmweaver.bsky.social Labs! Abbey Vito’s first publication discusses a tug-of-war between BER enzymes and chromatin for access to damaged DNA, a battle central to genome stability. Built on Tyler's foundational work! tinyurl.com/ywkt2bt5

Happening this week! Register for the Thursday October 30 Virtual Office Hour on Structure Summary Pages Learn how a exploring a single PDB structure at RCSB PDB can lead to visualizations, analyses, and searches across the archive

Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing Read more: www2.mrc-lmb.cam.ac.uk/a-new-method... #LMBResearch

Another one!

Great info here!

In another preprint from the @brianhie.bsky.social Lab and @synbiogaolab.bsky.social, they introduce Germinal, a generative AI system for de novo antibody design. Germinal produces functional nanobodies in just dozens of tests, making custom antibody design more accessible than ever before.

Love this pipeline! Congrats to all involved!

🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM. nimgs.zoom.us/webinar/regi...

Major update from #SCIMaP... FY26 House district level scorecards. Our FY26 budget analysis tab includes clickable district info, breaking down the expected economic and job loss given the White House's budget proposal that would slash NIH research funding by >40%. scienceimpacts.org/fy26

Just made my first webapp mostly for analyzing structures from #BindCraft 👉🏼 pdb-viewer.lovable.app It’s bare 🦴 at this point but curious if others find something like this useful and features they’d like? 🧬🧶

We’ve refreshed the AlphaFold Database Entry Pages! Explore the cleaner design, improved 3D viewer, and easier access from any device 👇 www.alphafold.ebi.ac.uk/entry/P04637 Or read more👇 www.ebi.ac.uk/about/news/u... #AlphaFold #Bioinformatics #UXForScience @pdbeurope.bsky.social

Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination www.biorxiv.org/content/10.1101/2025.07.31.667873v1 #cryoem

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1... Happy testing: cryosift.org #cryoEM #CNN

NEW from me at STAT: We've learned that DeepMind is going to fund CASP, the protein structure competition that brought Google DeepMind's #AlphaFold to prominence (and a Nobel Prize), as its NIH funding runs out. More @statnews.com: www.statnews.com/2025/07/21/c... 🖥️🩺🧪🧬 #bioML

Designed protein pockets recognize intrinsically disordered protein regions @science.org www.science.org/doi/10.1126/... www.science.org/doi/10.1126/... www.statnews.com/2025/07/17/n...

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

CASP is the main reason the protein structure prediction technology and research field advanced over the last 30 years. And the main reason AI based methods have been accepted and widely applied in biology. So shortsighted of NIH to postpone or even halt funding. John Moult is a scientific hero.

We have written up a tutorial on how to run BindCraft, how to prepare your input PDB, how to select hotspots, and various other tips and tricks to get the most out of binder design! github.com/martinpacesa...

🧪 We're beta testing - and we want your thoughts! Try the redesigned PDBe entry pages & let us know: 🔸 What’s working? 🔸 What’s confusing? 🔸 What features do you miss? 💬 Feedback form: tinyurl.com/pdbe-beta-form #PDBeBeta #Bioinformatics #Biochemistry #Science #UserVoice

The videos for the recent CCP4 Study Weekend 2025 are now available on YouTube. www.youtube.com/playlist?lis...

Fantastic day sharing knowledge with the brilliant researchers at NIH who valiantly work to advance the frontiers of science for the cause of improving human health. Thank you again for your service 🙏 🇺🇸

Waiting for binder design results… #denovo #proteindesign

The cryo-em core at kumc is looking for a lab manager. This is an exciting opportunity to join a growing facility. kumc.wd5.myworkdayjobs.com/en-US/kumc-j...

Happy to share we got the NoA for the NIH S10 proposal I submitted for a Glacios #cryoEM 🔬 for #IowaResearch #structuralbiology

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

11th NIH R25 Train the Trainer SBGrid Consortium workshop at @harvardmed.bsky.social-this one specifically for the #CryoEM Facility leaders. #RELION & #CryoSPARC in @amazonwebservices.bsky.social (AWS) cloud environment. Melissa Chambers & Daniel Stoddard will co-lead the new CryoACCESS initiative.

Do you want to visualize motion in protein structures? I've used @chimerax.ucsf.edu and little python code to do just that github.com/schaefer-jh/.... Here is some ABC transporter magic. #LLM assisted #coding

Introducing Extended PDB IDs at RCSB PDB Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued. www.rcsb.org/news/fe...

Thanks to @rcsbpdb.bsky.social for a new way to enjoy my morning ☕️ #structurefunction

Next up? 👀 @als-lbnl.bsky.social

Working with an interdisciplinary team, we have developed a website to communicate how the White House's proposed cuts to health research would cause losses of $16B and 68,500 jobs. Find out how your community may be impacted. Explore more at SCIMaP: scienceimpacts.org a 🧵

Morning ☕️ just got a visual upgrade! #bottomless #gaggia

An email I look forward to getting! #BindCraft #ProteinDesign Will have to optimize some settings to get more designs 💻

The final piece of my postdoctoral work in the @freudlab.bsky.social was recently published! Quite amazing what this small little repair polymerase can do to fill 1-nt gaps in chromatin. www.nature.com/articles/s41...

My S10 proposal finally got a new advisory council date. (Originally early Feb)

The ever popular EMBO practical course in cryoEM image processing, to be held at Birkbeck College London from 9th to the 16th September, is now open for registrations meetings.embo.org/event/25-cry...

Another great job at my favorite university: UNC-Chapel, as new Director of the UNC Macromolecular Interactions Facility. Our outstanding Director is retiring. The Director is responsible for 1) core facility management, 2) training, & 3) graduate teaching 1/n unc.peopleadmin.com/postings/297...

Iowans! Your government is coming for mRNA vaccines today. You can submit a comment here to let them know why this is wrong and why these vaccines are critical.

🚀 #AlphaFold Database update AlphaFold DB now integrates The Encyclopedia of Domains (TED) – a resource designed to systematically identify & classify structural domains within AlphaFold-predicted protein structures. www.ebi.ac.uk/about/news/u... @pdbeurope.bsky.social

A giant of modern crystallography has passed. George Sheldrick was the author of the SHELX series of programs (among others) for structure solution and refinement of both small-molecule and macromolecular crystal structures. www.chemistryworld.com/opinion/shel...

Structural biology is in an era of dynamics & assemblies but turning raw experimental data into atomic models at scale remains challenging. @minhuanli.bsky.social and I present ROCKET🚀: an AlphaFold augmentation that integrates crystallographic and cryoEM/ET data with room for more! 1/14.

Meet BioEmu-1 from Microsoft Research. This deep learning model can generate thousands of protein structures per hour, unlocking new possibilities for protein scientists and drug discovery and research. www.microsoft.com/en-us/resear...

Core facilities have such a huge impact on research success across universities but there are many other important roles as mentioned here. This will cause enormous damage to life changing research in addition to job cuts across the board.

“I’ve spent over five decades as a scientist in academia & the federal government, including as director of the National Institutes of Health. Never before have I seen my profession so politicized as it is now under the Trump administration” #AcademicSky 🧪 www.nytimes.com/2025/02/14/o...

Research America has created an action item link that sends 3 letters to support #NIH to your US Senators and Representative based on your zip code. It takes only a minute or two to fill out. You don’t need to be an academic to support #Science! advocacy.charityengine.net/Default.aspx....

A new paper reports a strategy to induce liquid-liquid phase separation in E. coli to enhance the performance of artificial metalloenzymes www.nature.com/articles/s41...

An update to #AlphaFold3 server. Now, you can add any ligand from the PDB using a CCD Code (up to 2024_10_28). Here is an MPro #SARS-CoV2 inhibitor from PDB 8u9h. The crystal structure is grey, and the #AF3 model is colored by pLDDT.