Exciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).

Amazing new paper from Clair Baldock's lab resolves the collagen VI microfibril structure by cryo-EM to reveal a cysteine-rich coiled-coil crucial for heterotrimerization & microfibril assembly. The structure also reveals a hotspot of collagen VI muscular dystrophy mutations that disrupt assembly.

We have written up a tutorial on how to run BindCraft, how to prepare your input PDB, how to select hotspots, and various other tips and tricks to get the most out of binder design! github.com/martinpacesa...

🚀 Excited to release BoltzDesign1! ✨ Now with LogMD-based trajectory visualization. 🔗 Demo: rcsb.ai/ff9c2b1ee8 Feedback & collabs welcome! 🙌 🔗: GitHub: github.com/yehlincho/Bo... 🔗: Colab: colab.research.google.com/github/yehli... @sokrypton.org @martinpacesa.bsky.social

New collaborative paper between JPArmache and Bowman (@bowmanlab-jhu.bsky.social) labs show how the yeast CHD1 chromatin remodeler depends on activator elements to distort nucleosomal DNA. This explains how the NegC inhibitor blocks activity. www.nature.com/articles/s41...

Very excited that our work describing hu.MAP3.0 is published in @molsystbiol.org. Here we use machine learning to integrate >25k mass spectrometry experiments to place ~70% of human proteins into 15k protein complexes. www.embopress.org/doi/full/10....

Amazing #cryoEM structures and incredible community here at @cniostopcancer.bsky.social for the “machines acting on DNA and RNA” congress!

Super happy to share the final shape of our work describing the molecular #structure and #enzymatic mechanism of human #collagen #galactosyltransferase #GLT25D1 published in @natcomms.nature.com. Want to know how collagen become sweet? read the 🧵 below!

We are thrilled to announce the 2025 EMBO practical course in cryo-em image processing, Birkbeck College, London, 9-16 Sept 2025. More info & apply: meetings.embo.org/event/25-cry... Organisers Giulia Zanetti & Helen Saibil. Beautiful image from co-organizer @carolynmoores1.bsky.social lab.

This is a masterpiece ! #cryoEM Structures of H2A.Z-associated human chromatin remodelers SRCAP and TIP60 reveal divergent mechanisms of chromatin engagement www.biorxiv.org/content/10.1...

Did you make our cryo-EM webinar last week? Dive into pre-processing, how to interpret 2D classes, 3D reconstructions from the initial map to final structure with CSO Giovanna Scapin. Catch it here: youtu.be/2dBnPVkaoFs Sign up for Overcoming Limitations, on 3/25: nimgs.zoom.us/webinar/regi...

📢 Registration is now open for 'Wellcome-MRC Cryo-EM in Structural Biology 2025'! 📅 Join us online March 3-7 at @diamondlightsource.bsky.social @universityofleeds.bsky.social for lectures & demos in #CryoEM & #CryoET: sample prep, SPA, model building & more!🧬 🔗 www.diamond.ac.uk/Instruments/...

Been thinking about creating a collection of good protein structure figures, as inspiration for my own work. #1 www.nature.com/articles/s41...

I’m happy to share gapTrick, an AlphaFold2-based tool for characterising protein-protein complexes www.biorxiv.org/content/10.1...

#SIRT7 is a histone deacetylase with highly specific activity on #chromatin substrates. We just published mechanism-based #cryoEM structures of #SIRT7 on nucleosomes to understand its activity 👇 www.nature.com/articles/s41... (1/8) #ChemBio #ChemSky

Cool event, great people! Thanks for putting us together @fornerislab.bsky.social and @thermofishersci.bsky.social!

Exciting new preprint alert! 🚨 The Collepardo lab, teaming up with Huabin Zhou and the Rosen lab we took a deep, high-resolution look into chromatin condensates! [1/6] #Chromatin #MD @rcollepardo.bsky.social @juliamaristany.bsky.social www.biorxiv.org/content/10.1...

Truly honoured to be the first user of such an amazing platform! For sure a reference point for all the Italian community. Exciting time ahead! ❄️🚀

Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription. www.nature.com/articles/s41...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint: www.biorxiv.org/content/10.1... Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com #TeamTomo

Safer, nonradioactive staining alternatives for electron microscopy are introduced, simplifying sample preparation, reducing costs and making structural biology more accessible to researchers globally #NegativeStaining #ElectronMicroscopy doi.org/10.1107/S205...

1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM. www.youtube.com/playlist?lis...

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social! tinyurl.com/setd2