7. For scientific progress and OUR understanding of the world this is of course very bad news. The only way to change this is to refocus on discovery NOT being prolific. In our days Galileo would have had not time to look at Jupiter, he would have reported telescopes in different colors instead.

This is an interesting idea. Since journals are keen to make authors declare when they have used AI, might authors want editors to do the same...?

If you posted your preprint under a CC-BY license, you already gave permission to this kind of re-use, whether you like it or not. But very relevant question for preprints posted with the option "all rights reserved", or under a CC license with clauses NC (non-commercial) and/or ND (no derivatives).

It is just so unfortunate that you chose to fuel a similar kind of "top dog / alpha male" culture by using language (1%) that is associated with extremely rich people whose ethics are questionable at best. I am actually excited about what you are doing with QED, this "best" papers thing...

🚀 Can automation scale #cryoEM? We recently demonstrated it is possible to make #cryo-EM more routine and scalable testing across 21 challenging #GPCR datasets, a real-world scenario for #SBDD, achieving results equal or better than manual processing. Read more 👉 cryosparc.com/blog/automat...

#cryoem folks- we keep seeing this popping up in 2d classes. Sample is a recombinant complex from insect cells

We are sharing a tool developed initially for internal use called VOSE (Volume Operations Simplified Edition) today. It is written specifically to work in ChimeraX (version 1.5+), with no additional libraries necessary to be installed. github.com/jparmachelab...

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

I learned so much from watching past editions of @ccpem.bsky.social Spring Symposium, so I agreed to my talk being recorded even though this is stepping well out of my comfort zone (public speaking isn't my strongest skill). Anyway, here is the recording: www.youtube.com/watch?v=AKTf... #CCPEM

The next #CryoEM Current Practices Webinar speaker will be Mark Herzik from UCSD speaking on "Old tools, new tricks: engineering better cryoEM sample preparation workflows through design and chemistry" 6/25/2026 at 12 PM ET / 9AM PT Register here : us02web.zoom.us/webinar/regi...

If you know someone who would be a good cryoEM facility manager, please encourage them to apply at jobs.rutgers.edu/postings/273... .

The DiffUSE Project wants examples where structural heterogeneity matters but cannot be encoded in PDBx/mmCIF. X-ray, cryo-EM, time-resolved, multi-map, or fragment screening data welcome. Submit yours: diffuse.science/submit/

Cryo-EM structure of ALC1 in an open conformation bound to a PARylated nucleosome pubmed.ncbi.nlm.nih.gov/42159202/ #cryoem

It is beyond me why anyone would want to automate away one of the most exciting parts of this job. "Let AI play your video games so you don't have to." You absolutely can't become an expert model builder without doing the "grunt work". Who will evaluate the results if there are no more experts?

New preprint🧊🔬Introducing LowDoseWizard—a guided SerialEM workflow for rapid, standardised cryo-TEM setup! Setting up low-dose can be slow & daunting, especially for new users. LowDoseWizard automates optics, camera, and alignment steps from minimal input — full setup in <15 min. #cryoEM #SerialEM

Very interesting to see that it is the same "difficult" particles that trip up classification methods with completely different underlying formalism. Also great to now have a dataset with known ground truth assignments!

🔭 Astronomer Cecilia Payne-Gaposchkin made one of the most fundamental discoveries in the history of science: stars are made primarily of hydrogen & helium. Her 1925 PhD thesis is widely regarded as the most brilliant ever written in #astronomy. She was born #OTD in 1900. #WomenInSTEM #AstroSky

Finally we can study interesting things (like chromatin 😇) in situ, beyond boring ribosomes. 😜

A 37-million-particle dataset from over 250 experiments to accelerate data-driven cryo-EM analysis www.biorxiv.org/content/10.64898/2026.04.29.720997v1 #cryoem

Structural Insights into the Coupling Mechanism of Vectorial CO2 Uptake by DAB1 www.biorxiv.org/content/10.64898/2026.05.01.722082v1 #cryoem

I noticed that the results of CS denoising were good, but a bit stochastic (some particles were gone in one run, but clearly visible in another). Combining the results of multiple denoising models by taking the minimum value at each pixel helps. github.com/olibclarke/c...

Been training for this moment my whole life.

@olibclarke.bsky.social finally listened to your ccpem talk, the calmodulin result is very intriguing! I wonder what you would get with data from a more recent detector as you said, but also from a HexAuFoil grid. The example of CODH (Emma Hesketh's talk) suggests it helps for small particles.

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Gentle reminder: May 1st is not about work. It’s about workers. Happy International Workers’ Day.

New Preprint alert! Excited to share our latest work on the #MAPKinases from stellar PhD student Jill von Velsen. First structures of the MAP2K MEK1 activating its substrate MAPK between 2.9 and 3.6A - amazing resolutions for such a small and mobile complex! www.biorxiv.org/content/10.6...

If you're curious about how cells quickly eliminate misfolded proteins produced under proteotoxic stress or mitotic substrate during cell cycle progression, check out our latest article in Nature Communications: doi.org/10.1038/s414...

We just preprinted one of my favorite studies @FlatironInst . I was lucky to be part of an amazing team studying the effects of rapid cooling to preserve samples in cryoEM. Read on to learn about the limits of cryoEM for biophysics and how to overcome them www.biorxiv.org/content/10.6...

We're up and running on the last day of the Spring Symposium. More great science after a lively dinner last night.

Molecular mechanism of redox regulation of the alpha-carboxysomal carbonic anhydrase CsoSCA www.biorxiv.org/content/10.64898/2026.04.02.716132v1 #cryoem

Our new preprint on detailing the molecular mechanisms of how the carbonic anhydrase activates it's activity upon encapsulation into alpha-carboxysomes is now live. Congrats to Nikole and @guillawme.fediscience.org.ap.brid.gy !

Molecular mechanism of redox regulation of the alpha-carboxysomal carbonic anhydrase CsoSCA From the incredible team of @cblikstad.bsky.social www.biorxiv.org/content/10.6...

Molecular mechanism of redox regulation of the alpha-carboxysomal carbonic anhydrase CsoSCA https://www.biorxiv.org/content/10.64898/2026.04.02.716132v1

Molecular mechanism of redox regulation of the alpha-carboxysomal carbonic anhydrase CsoSCA https://www.biorxiv.org/content/10.64898/2026.04.02.716132v1

1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).

You asked, we listened. Millions of AI-predicted protein complex structures are now available in the #AlphaFold Database. This spans homodimers from 20 of the most studied species, including humans, as well as the World Health Organization’s priority pathogens list. www.ebi.ac.uk/about/news/t...

AlphaFold database has entered the era of complexes. Together with NVIDIA, DeepMind and EBI, we use ColabFold, OpenFold and MMseqs2-GPU to predict ~31 million complexes (homo & hetro-dimers) resulting in 1.8 million high-quality predictions 📄 research.nvidia.com/labs/dbr/ass... 🌐 alphafold.ebi.ac.uk

“think about whether some of the proprietary license money you’re currently spending could be spent instead on open solutions that would be yours forever, endlessly adaptable, and benefit the entire world. And please get in touch by emailing [email protected]” 👏 👏 👏 Can’t wait to see where this goes

Hello, world! We are starting a worker cooperative to build, maintain, teach, and support open source scientific imaging software. image.coop/blog/posts/2...

I was skeptical of Bluesky from the start, especially because its decentralized nature didn't seem easily actionable in practice, and because its main (and, for a while, only) personal data server (PDS) is hosted in the US (which inspires less confidence every day, these days...).

Question for the #cryoEM crowd: have you played with ModelAngelo, CryoAtom and EMProt, and would you care to offer thoughts/notes on the comparison? I have ModelAngelo installed and I love it, but the other two claim improved performance - which should I try first?

The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....

I don't envy people who start their PhDs now. What they should be trying to do - I think - is to first become confident experts in certain things, and this might involve using as little AI as possible not to become dependent or have their view distorted.

ALC1 Finds a New Foothold on the Nucleosome's Super-Groove www.biorxiv.org/content/10.1... #cryoEM

Atomic resolution structure of spinach rubisco reveals protons and dynamics www.biorxiv.org/content/10.64898/2025.12.22.696010v1 #cryoem

My hero!!! An amazing scientist and a wonderful person!