A closer look at this helix indicates that it is rich in basic residues: Lys and Arg account for 34% of its sequence composition. This already suggests that its function is to interact with DNA. Looking at sequence conservation, it seems like the basic […] [Original post on fediscience.org]

So what does this new structure look like? ALC1 is loosely bound to the #nucleosome, in a conformation that likely doesn't allow it to slide the nucleosome along the DNA, since the two ATPase lobes are not clamping the DNA. But we finally see the macro […] [Original post on fediscience.org]

And here is @HRBridges magic explained in a flow chart. ✨ For more info, consult the preprint's methods section and the case study in the CryoSPARC Guide. This is a compact view of the process, showing what worked best. The map we deposited had job number J8039! 😵‍💫 3/5

In 2021, I was involved in a study of the #chromatin remodeler ALC1, a protein involved in the early events of the DNA damage response. This led to a paper summarized in a previous thread: https://fediscience.org/@Guillawme/111534984107819771 We had deposited the raw #CryoEM data to #empiar […]

As promised, here is a summary of this new preprint on the ALC1-nucleosome complex. 🧵 https://doi.org/10.1101/2025.11.10.687450 First, a reminder of some structural features of the #nucleosome. Histones H2A and H2B form a negatively charged cleft exposed […] [Original post on fediscience.org]

Atomic resolution structure of spinach rubisco reveals protons and dynamics @cblikstad.bsky.social www.biorxiv.org/content/10.6...

RE: https://fediscience.org/@Guillawme/111534984107819771 Something really cool happened to me this year! @HRBridges re-processed a #CryoEM dataset from some previous work of mine and colleagues (publicly available as EMPIAR-10739; see quoted post below for a summary of this work). She […]

Confused by all the histones that are cropping up in organisms that are decidedly NOT eukaryotes? check out our review - fantastic work by team NucEvo in the #Lugerlab The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...

ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1

On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look? Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner! guide.cryosparc.com/processing-d...

ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1

Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem

If you ever thought science was too tame a career, how does kite skiing 4,000km across Antarctica over 3 months sound? #antarctica #adventure #expedition #science #climatechange

HHMI adopts Plan U journals.plos.org/plosbiology/...

@nucleosomepolice.bsky.social do you think this newly discovered giant virus has nucleosomes? doi.org/10.1038/s414...

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

Great point. Make it opt out, not opt in, for authors to see feedback. We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose. How can we do that if public reviews are not even seen by the authors?

Why hasn't Preprint Peer Review caught on more? I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs. more in thread below!

Thanks for sharing @olibclarke.bsky.social. I wonder if many more "hollow" complexes are co-purified with binding-partners. Let's all put more datasets on EMPIAR to revisit processing with new creative approaches like Oli's.

In the Bay Area? Love computational cryo-EM/ET? Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community! Details and registration at frostbyte-cryoem.github.io Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

Come join us as a postdoc! We have an open position in biochemistry of cyanobacterial CO2 fixation focusing on regulation of carboxysome function! Please help me spread the word and welcome to apply! www.blikstadlab.org/open-positio...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

Is it me or is this place really taking off? Shall we all take a step closer to full sustainable independence and try Mastodon too!

Since the beginning of Bsky I've been skeptical it's a good replacement for Twitter. It's already taken investor money, and when the return on investment comes due, we'll get either: ads, paid accounts, sell-out to some richer tech bro. Or a combination of these unpleasant outcomes.

My fabulous postdoc worked SO hard on a fellowship application (submitted today) and I am so proud of them. y'all reviewers, BE KIND and if you must, offer criticms that is at least constructive! Consider that there are differences in life experiences. Not everybody has a straight trajectory. 🧪✍️

Summary of this new paper 🧬🧶 on Mastodon: fediscience.org/@Guillawme/1...

Summary of this 2021 paper 🧬🧶 on Mastodon (I wrote it before bsky was out of invitation-only, I have no motivation to copy/paste it all here, and I still think Mastodon is a more sustainable platform than bsky anyway): fediscience.org/@Guillawme/1...

"Is X-ray crystallography dead?" some asked when cryoEM got momentum. Depends on what you work on. For nucleosomes it certainly looks like it is dead: all structures with nucleosomes released in 2023 were cryoEM structures! @nucleosomepolice.bsky.social guillawme.github.io/insights-fro...

New paper published! 😊 Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶 doi.org/10.1038/s414...

What about cross-posting on mastodon? It's not a closed community etc etc. I'm only here because sci-twitter seems to have migrated here. I think mastodon is a much better solution than the options brought to you by the letters X, B, T, or S.