Excited to share our preprint📯: We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬 Big thanks to Nadine Wolgast & team @ www.uksh.de , catchall-kfo5010.com! See: doi.org/p7k5 and 🧵

Big shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com

Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors https://www.biorxiv.org/content/10.1101/2025.09.22.677496v1

Check out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells! ‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕

This has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes. I feel tempted to think about one-pot PIZZA 🍕 next! First authors Isabell and @therealjonaskath.bsky.social were amazing!!

Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀 Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝 = Programmable And Site-specific Transgene Addition. 😎

You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪

🚀 In preparation for releasing two new stories, @therealjonaskath.bsky.social is revisiting the journey so far—sharing the lab’s last six papers. What a ride it’s been… 🙌🧬🧪🧫

🚨 Paper alert! "Next-gen multiplex-edited CAR-NK cells: more edits, more power?" @jitc @bmj.com With @dlwagner.bsky.social we discuss Wang et al.’s landmark paper on multiplex base-edited non-viral CAR-NK cells. Promising efficiency but safety challenges! jitc.bmj.com/content/13/9...

Plasmacytoid dendritic cells can transition to become cDC2s under to influence of TNF, while type-I interferon reduces TNFR expression and thereby inhibits transition. These transitioned cDC2 seem fully functional. 🧪 ⑂ Hornero et al 2025 in Nat Immunol www.nature.com/articles/s41...

23 tools to work with (single-cell) TCR/BCR-seq immune repertoire data 🧵 👇

Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...

Horisberger et al separate Lupus nephritis patients in 3 groups by blood: healthy-like, interferon-dominant and CD8 T cell-dominant. The CD8 T cell-dominant group had the highest biopsy activity score and highest response rate after 1 year; the healthy-like the most chronic damage. 🧪 ⑂ #NephSky 1/

A synonymous mitochondrial variant is negatively selected against in short-lived CD8 effectors – How? The variant changes tRNA pairing from Watson-Crick-Franklin to wobble, thereby reducing translational efficiency in high energy demand. 🧪 ⑂ Lareau et al – 2025 www.pnas.org/doi/10.1073/...

🚨 First-ever FOCIS Journal Club! 🎓 Blood Immunophenotyping Identifies Distinct Kidney Histopathology and Outcomes in Patients with Lupus Nephritis 🗓️ Oct 13 | 🕚 11 AM CT 🎤 Dr. Rao & Dr. Horisberger 💥 FREE for all! (Non-members need code)

So accurate, it hurts

Journals in August

In Lupus nephritis patients, neutrophils have increased surface LAMP1, which is externalized after activation. Soluble LAMP1 is increased both in the blood and urine of patients, correlating with histological activity. #NephSky 🧪 Our paper in @eurjimmunol.bsky.social doi.org/10.1002/eji....

#genetherapy #celltherapy

Q4ddPCR (May the Fourth Be Precise): A Flexible, 4-Target Assay for High-Resolution HIV Reservoir Profiling https://www.biorxiv.org/content/10.1101/2025.07.28.667202v1

My colleagues at Nature Biomedical Engineering @natbiomedeng.nature.com are hiring an associate or senior editor. Ideal applicant will have expertise in immunology, immunotherapy, or immuno-oncology. Please feel to ask me questions! springernature.wd3.myworkdayjobs.com/SpringerNatu...

Resting MAIT cells generally express the non-cytotoxic Granzyme K, but rapidly switch to the cytotoxic Granzyme B upon stimulation. Consequently, previously stimulated MAITs kill much more target cells than resting ones. 🧪 Kurioka et al – 2015 – @mucosalimmunol.bsky.social doi.org/10.1038/mi.2...

It's my first #newPI roll call on BlueSky! Calling all new #cancer PIs who just started or who are about to start their labs! Please say hi and what you work on so I can follow you if I don't already! 😃

Bye forever, WeTransfer.

Oxidized mitochondrial DNA induces NLRP3 activation in pDCs. This leads to enhanced pDC function beyond IFNa production so that they produce IL1b, IL6 and IL21, inducing Tfh differentiation and autoimmunity (in 🐭). Xian et al – 2025 @natureportfolio.nature.com www.nature.com/articles/s41... 🧪⑂

Individuals of subsaharan ancestry often have a Duffy Null blood group. DARC (the Duffy protein) is a multi-chemokine sink on red blood cells. Duffy Null people have lower peripheral blood neutrophil counts, likely resulting from increased neutrophil egress into the periphery. 1/2

Regulatory T cells mitigate cardiac fibrosis and dysfunction in familial hypertrophic cardiomyopathy by modulating chronic inflammation www.science.org/doi/10.1126/...

We'll know that AI companies are shifting "AI will cure cancer" from vague hype to actual ambition when they start quietly spending a fortune on oligo and peptide synthesis.

Very excited to help lead this effort with @casanovalab.bsky.social - working with IAPIDS this will be a premier home for important work in the field of human immunity and inborn errors of immunity

A CAR T platform using engineered protein G variants and Fab scaffolds enables safe, adaptable, multi-targeted cancer therapy www.science.org/doi/10.1126/...

I thought my scRNA data is bad with all the plasmacytoid dendritic cells expressing Granzyme B. Turns out, human pDCs are known producers of Granzyme B and deliver it to T cells, inhibiting T cell expansion. 🧪⑂ Jahrsdörfer et al 2010 in @bloodjournals.hematology.org doi.org/10.1182/bloo...

Excited to join the Stem Cell Reports team as an Early Career Editor! Grateful for the opportunity to contribute to the journal alongside fantastic colleagues. I encourage you to submit your work for consideration! @isscr.org @stemcellreports.bsky.social

NR4A2 & RGS16 promoters support delivery of cytokines (e.g. IL-12) directly to the tumor site, leading to enhanced antitumor efficacy and long-term survival of mice in both syngeneic and xenogeneic models @nature.com @paulbeavis4.bsky.social www.nature.com/articles/s41...

Can peer review be done faster, without compromising on quality? Biology Open has embarked on an exciting experiment to find out - Editor-in-Chief Dan Gorelick @danielgorelick.bsky.social explains. www.youtube.com/watch?v=Tt5h...

Succinate induces Treg dysfunction by inhibiting succinyl-CoA generation. Succinyl-CoA is necessary for FOXP3 succinylation and protection of FOXP3 from proteasome degradation. Wang et al – 2025 @natureportfolio.nature.com www.nature.com/articles/s41...

Dimi @dlwagner.bsky.social showing how TruC edited Tregs have superior properties compared to CAR-Tregs for use in cell therapy. #FOCIS2025 🧪

We would like to acknowledge the consistent support of the baristas at #FOCIS2025 in Boston. Keeping us fueled through all the great science over the last few days. @focisimmunology.bsky.social 🧬🧪☕️

Deepak Rao receives the Lupus Insight Award at the #FOCIS2025 following his insights on the IFN-induced dysbalance of Tph and Th22 cells in SLE. 🎉🧪

Hey bioRxiv Affiliates - we have a backlog again. If any affiliates are around to screen, we'd be immensely grateful - more importantly the authors in the queue would be too!

Loving that immunologists at #FOCIS2025 are on @bsky.app 🤓🥳🧬 Great way to find who to follow… @focisimmunology.bsky.social

#genetherapy #celltherapy

Just published @science.org Engineering T cells within the body, a big step forward for treating cancer and autoimmune diseases, as demonstrated in animal models. Uses the mRNA + nanoparticle platform www.science.org/doi/10.1126/... www.science.org/doi/10.1126/...

Mice with low fecal IgA might be colonized be the auxotrophic Tomasiella immunophile which degrades kappa LC immunoglobulins in gut and induces disease-relevant reductions in intestinal IGs. 🧪⑂ Lu et al 2024 @science.org www.science.org/doi/10.1126/...

Join us June 24-27, 2025, in the vibrant city of Boston, MA! If you haven't already, we recommend registering early. Rates go up the longer you wait! You can find all the details, including registration information, on the FOCIS 2025 website: zurl.co/vV0m6

Both @immunoboys.bsky.social will be at FOCIS next week - looking forward to cool science in beautiful Boston. 🧪🔬

Human genetic variation reveals FCRL3 is a lymphocyte receptor for Yersinia pestis www.cell.com/cell-genomic...

🧬 🧪 👇

How to find Evolutionary Conserved Enhancers in 2025? 🐣-🐭 Check out our paper - fresh off the press!!! We find widespread functional conservation of enhancers in absence of sequence homology Including: a bioinformatic tool to map sequence-diverged enhancers! rdcu.be/enVDN github.com/tobiaszehnde...

Targeting Regnase-1 unleashes CAR T cell antitumor activity for osteosarcoma and creates a proinflammatory tumor microenvironment www.biorxiv.org/content/10.1...

🚨 Our work on optimizing single-stranded DNA donors with Cas12a binding moieties is published at @moltherapy.bsky.social Nucleic Acids! 🧪 Since the preprint, we added two super interesting findings. Short 🧵👇 (1/5) www.cell.com/molecular-th... #ImmunoSky #GeneEditing #CARTcells