Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...

🚨This week in @natcomms.nature.com: We resolve an enduring enigma in T cell biology—how TCR binding to p/HLA triggers intracellular signaling. CryoEM in native environment reveals a 'jack-in-the-box' mechanism! Collab btw @rockefeller.edu @mskcancercenter.bsky.social rdcu.be/eUPPl

Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation https://www.biorxiv.org/content/10.64898/2025.12.10.693159v1

Original work here ⬇️, led by the inimitable Lorenzo Brunetti and Michael Gundry. This work became foundational in the field.

Today's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).

A big advance, folks, for off-the-shelf, universal, engineered (base-edited) T cells achieving complete response vs incurable leukemia in several patients www.nejm.org/doi/full/10....

Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky authors.elsevier.com/sd/article/S...

Extrusive and cohesive cohesin cooperate to repair double-strand breaks in DNA @science.org @albertomarin.bsky.social @gerlichlab.bsky.social @imbavienna.bsky.social www.science.org/doi/10.1126/... www.science.org/doi/10.1126/... www.science.org/doi/10.1126/...

Big news! Our team developed Neuro-PERC, a non-viral CRISPR delivery platform for genome editing of the brain. In our new preprint - bit.ly/NeuroPERC_pre - we report efficient editing in multiple models & species, including field-first results in large animals (pigs) 🧵👇

On this #WorldAIDSday @cgaebler.bsky.social, Olaf Penack & teams at #CharitéBerlin report the #SecondBerlinPatient cured of #HIV after heterozygous CCR5Δ32 HSCT. The study opens exciting perspectives for immune-based #HIVCure strategies for longterm ART-free viral control and ultimately cure.

Today’s xkcd made me cry. In a good way. xkcd.com/3172/

Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation. 2025 Sci Transl Med 🧪 ⑂ www.science.org/doi/10.1126/...

What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)? In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13. www.biorxiv.org/content/10.1... 1/n #Nephsky 🧪

A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com www.nature.com/articles/s41...

(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.

Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling. LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop. 🧪 ⑂ 1/2

Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...

A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

What is a promoter? And how does it work? We very happy to share our latest work trying to understand enhancer-promoter compatibility. I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters www.biorxiv.org/content/10.1...

Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social. Full list: www.statnews.com/wunderkinds/ @statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social

“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa. Check out our new story: 👇 www.nature.com/articles/s41... One week after the Nobel prize for Treg 🍀

“Good things take time” Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function! A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social

HLA matching or CRISPR editing HLA-I/II enables engraftment & function of allo human Treg therapy in a humanized mouse transplantation model @natcomms.nature.com @dlwagner.bsky.social @bcmhouston.bsky.social www.nature.com/articles/s41...

@dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸 We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸 Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙

Accurate. #academia #academy #philsky #philosophysky #philosophy #science

TCR activation impairs CAR-T cytotoxicity against separate target cells https://www.biorxiv.org/content/10.1101/2025.10.07.680634v1

Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community! Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor (BEKI) + posters by Ana, Lily, Jonas and Laura

Excited to share our preprint📯: We introduce #IntegrateALL, an open-source RNA-seq pipeline for interpretable molecular diagnostics in acute lymphoblastic leukemia!🧬 Big thanks to Nadine Wolgast & team @ www.uksh.de , catchall-kfo5010.com! See: doi.org/p7k5 and 🧵

Big shoutout to the whole group who made #IntegrateALL possible - especially @alinamh.bsky.social, Thomas Beder and Claudia Baldus at www.catchall-kfo5010.com

Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors https://www.biorxiv.org/content/10.1101/2025.09.22.677496v1

Check out one of our latest stories about combining our non viral knock-in strategy with integrases for an efficient, targeted integration of transgenes >8kb in human T cells! ‘One-pot’ PASTA: programmable and site specific transgene addition - all in just one reaction 🫕

This has been such a great collaboration with @dlwagnerlabs.bsky.social! You really need to check it out One-pot PASTA 🍝 - a one-step protocol to knock-in large transgenes. I feel tempted to think about one-pot PIZZA 🍕 next! First authors Isabell and @therealjonaskath.bsky.social were amazing!!

Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀 Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝 = Programmable And Site-specific Transgene Addition. 😎

You like engineering T cells, but you are missing the right flavor? Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1 We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt 𝐏rogrammable 𝐀nd 𝐒ite-specific 𝐓ransgene 𝐀ddition 🧪

🚀 In preparation for releasing two new stories, @therealjonaskath.bsky.social is revisiting the journey so far—sharing the lab’s last six papers. What a ride it’s been… 🙌🧬🧪🧫

🚨 Paper alert! "Next-gen multiplex-edited CAR-NK cells: more edits, more power?" @jitc @bmj.com With @dlwagner.bsky.social we discuss Wang et al.’s landmark paper on multiplex base-edited non-viral CAR-NK cells. Promising efficiency but safety challenges! jitc.bmj.com/content/13/9...

Plasmacytoid dendritic cells can transition to become cDC2s under to influence of TNF, while type-I interferon reduces TNFR expression and thereby inhibits transition. These transitioned cDC2 seem fully functional. 🧪 ⑂ Hornero et al 2025 in Nat Immunol www.nature.com/articles/s41...

23 tools to work with (single-cell) TCR/BCR-seq immune repertoire data 🧵 👇

Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...

Horisberger et al separate Lupus nephritis patients in 3 groups by blood: healthy-like, interferon-dominant and CD8 T cell-dominant. The CD8 T cell-dominant group had the highest biopsy activity score and highest response rate after 1 year; the healthy-like the most chronic damage. 🧪 ⑂ #NephSky 1/

A synonymous mitochondrial variant is negatively selected against in short-lived CD8 effectors – How? The variant changes tRNA pairing from Watson-Crick-Franklin to wobble, thereby reducing translational efficiency in high energy demand. 🧪 ⑂ Lareau et al – 2025 www.pnas.org/doi/10.1073/...

🚨 First-ever FOCIS Journal Club! 🎓 Blood Immunophenotyping Identifies Distinct Kidney Histopathology and Outcomes in Patients with Lupus Nephritis 🗓️ Oct 13 | 🕚 11 AM CT 🎤 Dr. Rao & Dr. Horisberger 💥 FREE for all! (Non-members need code)

So accurate, it hurts

Journals in August

In Lupus nephritis patients, neutrophils have increased surface LAMP1, which is externalized after activation. Soluble LAMP1 is increased both in the blood and urine of patients, correlating with histological activity. #NephSky 🧪 Our paper in @eurjimmunol.bsky.social doi.org/10.1002/eji....

#genetherapy #celltherapy

Q4ddPCR (May the Fourth Be Precise): A Flexible, 4-Target Assay for High-Resolution HIV Reservoir Profiling https://www.biorxiv.org/content/10.1101/2025.07.28.667202v1

My colleagues at Nature Biomedical Engineering @natbiomedeng.nature.com are hiring an associate or senior editor. Ideal applicant will have expertise in immunology, immunotherapy, or immuno-oncology. Please feel to ask me questions! springernature.wd3.myworkdayjobs.com/SpringerNatu...

Resting MAIT cells generally express the non-cytotoxic Granzyme K, but rapidly switch to the cytotoxic Granzyme B upon stimulation. Consequently, previously stimulated MAITs kill much more target cells than resting ones. 🧪 Kurioka et al – 2015 – @mucosalimmunol.bsky.social doi.org/10.1038/mi.2...