“Good things take time” Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function! A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social

@dlwagnerlabs.bsky.social on tour! 🌍🇩🇪🇺🇸 We had a great time at the Annual Congress of the European Society for Gene and Cell Therapy #ESGCT2025 in Sevilla. 🇪🇸 Super proud of everyone who presented their projects & grateful for working with this amazing team: science and good vibes! 🧬🧪💙

Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community! Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor (BEKI) + posters by Ana, Lily, Jonas and Laura

Super-large targeted Knock-Ins in primary T cells, with multigene inserts >8kb, in a single reaction. HOW?? 🙀 Combine simple CRISPR-HDR & Integrases for one-pot PASTA🍝 = Programmable And Site-specific Transgene Addition. 😎

Lab History pt. 4 (2024) What could be BETTER THAN TRAC KNOCK-IN ?? CD3Z-CAR KI story, published in Blood pmc.ncbi.nlm.nih.gov/articles/PMC...

Lab History pt. 3 (2023) Ultra-depletion of TCR+ cells from allogeneic CAR T cells to prevent GvHD.... LOG-FOLD BETTER THAN MACS!!! HOW? => Addition of short-lived TCR-specific CAR NK-92 cells during expansion. Published in Blood Advances 🔗 ashpublications.org/bloodadvance...

How everything started. My first 1st 🤟