Sophie G Martin (@sophiemartinlab.bsky.social)

On BioRxiv, the PhD work of Melvin Bérard, with help of Laura Merlini: TORC1 reactivation by pheromone signaling revealed by phosphoproteomics of fission yeast sexual reproduction. Enjoy the read! biorxiv.org/content/10.1...

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TORC1 reactivation by pheromone signaling revealed by phosphoproteomics of fission yeast sexual reproduction Starvation, which is associated with inactivation of the growth-promoting TOR complex 1 (TORC1), is a strong environmental signal for cell differentiation. In the fission yeast Schizosaccharomyces pombe, nitrogen starvation has distinct physiological consequences depending on the presence of mating partners. In their absence, cells enter quiescence, and TORC1 inactivation prolongs their life. In presence of compatible mates, TORC1 inactivation is essential for sexual differentiation. Gametes engage in paracrine pheromone signaling, grow towards each other, fuse to form the diploid zygote, and form resistant, haploid spore progenies. To understand the signaling changes in the proteome and phospho-proteome during sexual reproduction, we developed cell synchronization strategies and present (phospho-)proteomic datasets that dissect pheromone from starvation signals over the sexual differentiation and cell-cell fusion processes. Unexpectedly, these datasets reveal phosphorylation of ribosomal protein S6 during sexual development, which we establish requires TORC1 activity. We demonstrate that TORC1 is re-activated by pheromone signaling, in a manner that does not require autophagy. Mutants with low TORC1 re-activation exhibit compromised mating and poorly viable spores. Thus, while inactivated to initiate the mating process, TORC1 is reactivated by pheromone signaling in starved cells to support sexual reproduction. ### Competing Interest Statement The authors have declared no competing interest. https://biorxiv.org/content/10.1101/2024.06.04.597361v1