IsoNet2 determines cellular structures at submolecular resolution without averaging https://www.biorxiv.org/content/10.64898/2025.12.09.693325v1

Und hier sind sie – die 10 Preisträger*innen des Gottfried Wilhelm #LeibnizPreis' 2026 – ausgezeichnet für ihre exzellenten Forschungsarbeiten und Errungenschaften in der Wissenschaft! 🏆👏 Die Verleihung der Preise feiern wir am 18. März in Berlin. Einzelheiten & Kurzprofile: sohub.io/1uv1

New online: Atomic models of the Toxoplasma cell invasion machinery

Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase pubmed.ncbi.nlm.nih.gov/41361968/ #cryoem

Star student & postdoc Kiarash Jamali is setting up his own group at the Ellison institute in Oxford. Join him for the most exciting machine learning research in structural biology and beyond!! 🤩 #ProudPI

KI researchers have captured the first detailed molecular snapshots of human polynucleotide phosphorylase in action, revealing how this essential mitochondrial enzyme degrades RNA through an elegant base-flipping mechanism. Published in @narjournal.bsky.social 🧪 #mitochondria doi.org/10.1093/nar/...

📄 Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase ✍🏼 @bmhallberg.bsky.social @hdas29.bsky.social 📔Nucleic Acid Research @narjournal.bsky.social

DNA topoisomerase I acts as supercoiling sensor for bacterial transcription elongation pubmed.ncbi.nlm.nih.gov/41326756/ #cryoem

www.biorxiv.org/content/10.1... Example of Relion Blush regularisation combined with CryoDRGN to overcome orientation bias issues from Teder et al #CryoEM

We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...

Image of the Sun. Taken at night. Not looking up at sky but down through 8000 miles of rock to the other side of the Earth. Not with light but neutrinos.

my friend and wonderful colleague Stan is hiring at Genentech in South San Francisco! 🧬 🌉 Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility www.linkedin.com/posts/stanis...

EMBL scientists have new detailed info about Asgard archaea DNA structures offering important clues to more complex cell evolution. With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin. doi.org/10.1016/j.mo...

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated. mrc.tal.net/vx/appcentre...

The German company that makes the mechanical ladder used in the Louvre heist has used the image to advertise, with the text 'When you need to move fast' 10/10 response, no notes

Finally, just about 2 years after finishing the postdoc the last part of my work is finally online! We determined the structure of HSV-1 Origin Binding Protein, the viral initiator of DNA replication, by cryo-EM 🧪🧬🧶🧵 #cryoEM #structuralbiology

Using cryo-EM, we determined OBP structures at up to 2.8 Å resolution in multiple states: 1. bound to HSV-1 origin of replication (OriS) DNA 2. with a non-hydrolyzable ATP analog 3. as monomer, dimer, and higher-order assemblies

New cryo-EM structures of HSV-1 origin-binding protein reveal how herpesvirus initiates DNA replication, offering new antiviral targets beyond DNA polymerase. Study published in @narjournal.bsky.social 🧪 academic.oup.com/nar/article/...

I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.

📜 The Herpes simplex Origin Binding Protein: Mechanisms for sequence specific DNA binding and dimerization revealed by Cryo-EM 🧑‍🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social 📔 Nucleic Acids Research @narjournal.bsky.social 🔗 doi.org/10.1093/nar/...

Very excited that my final postdoctoral work is now online in Cell @cellpress.bsky.social. www.sciencedirect.com/science/arti... Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.

Locating the missing chlorophylls f in far-red photosystem I pubmed.ncbi.nlm.nih.gov/41066538/ #cryoem

How do SMC complexes load onto DNA to get ready for loop extrusion? @roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸‍♂️🤸‍♂️🤸‍♂️. Check out the new paper: www.cell.com/molecular-ce...

Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup). A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.

These results are truly impressive! But I do worry that #cryoEM is moving into a phase where progress is made by optimizing parameter settings for black box programs. The devil is often in the details, and I am not sure how this could be implemented in #relion, as stated, without more info.

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification https://www.biorxiv.org/content/10.1101/2025.09.14.676144v1

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data. Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...). Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å

#RNA25 @rna25.bsky.social