Meeting Report on the 1st GlyCosmos Symposium & Jamboree —Community-Driven Expansion of Glycan-Binding Molecules Resource#glycotimem#Glycobiologygy

Interesting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. doi.org/10.1021/acs.... #Chromatography #LCMS

In fermented foods microbes convert some of the food substrate into microbial protein. We found that in some fermented foods up to 10% of the total protein is microbial and that some of these microbial proteins have functions that might explain some of the beneficial effects of fermented foods.

In case you missed it, Thermo announced a new TSQ - the TSQ Certis. A few minor improvements: - 900 SRM/s - Compatible with the hotter HESI ion source Specification sheets absent, so unsure if it is an improved Altis Plus, or another TSQ. www.thermofisher.com/au/en/home/i...

Absolutely, and membership is still cheap. People could easily make an argument for their employer paying their dues and saving money. Back when I was a contractor I got a society membership benefit, so I paid ASMS dues myself (and had my employer pay the much-more-expensive ACS dues)

Delighted to share our new pre-print from PhD student Ziyun Zhang joint with @tracyh9494.bsky.social demonstrating that lung alveolar macrophages have a dense glycocalyx and interstitial macrophages do not, @mcrcellmatrix.bsky.social @lydiabeckeriii.bsky.social www.biorxiv.org/content/10.1...

This is pretty big, the membership definitely pays for itself now

Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures! Great colleagues, lots of cool projects… and you’ll get to work with me 😅 Please DM if you’re interested!

Tried Hypercarb PGC stationary phase but have been looking for another vendor's take on it? Millipore Sigma have just released their Carbon LC capillary flow columns recently: Available in 1 mm and 0.3 mm ID formats. www.sigmaaldrich.com/AU/en/substa...

Akkermansia muciniphila has potential as a next-generation #probiotic, but can it claim a niche and persist in a pre-existing, complex microbial community (in vitro)? 🦠🔍 #microsky #microbiomesky #glycotime link.springer.com/article/10.1...

An alternative to TMTpro: "Comparison of the single-cell proteomics revealed that the relative quantification made by IBT-16plex was comparable with that produced from TMTpro-16plex."

We're so excited to share our published new paper on MUC17-targeting CAR-T cells for gastric cancer! MUC17 is a safe, tumor-specific mucin. Our MUC17 CAR-T cells show strong antigen-specific killing and in vivo tumor control. Thank you, Marcela, for the support! jitc.bmj.com/content/13/1...

New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!

Exciting new paper from Sciex: “We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn)” Hopefully commercially available soon, the ability to do MSn is invaluable in characterisation workflows.

Happy to share our lab’s latest paper in Analytical Chemistry! pubmed.ncbi.nlm.nih.gov/41084887/ Our first foray into top-down proteomics led by Dr. Mozhgan Boroumand sought to identify proteoforms of the Senescent Secretome (SASP)-a stepping stone toward human proteoform biomarker candidates. 1/n

Please sign. This stupid decision needs reversing actionnetwork.org/petitions/cs...

We wrapped up #THEProteomicsShow Alt Proteomics season with Tim Cleland @paleoproteomicist.bsky.social talking paleoproteomics with me and Ben @proteomicsnews.bsky.social. Won't lie, this season was a blast talking about less typical "proteomics". Find it wherever you find fine podcasts or here.

An insightful patent, just from the drawings alone: ELECTRO SPRAY IONIZATION WITH CONDUCTIVE PEEK VOLTAGE APPLICATION patentscope.wipo.int/search/en/de... - Features an exchangeable emitter for prolonged column lifetime - Replacing the metal aspect of the emitter with carbon makes it biocompatible

Now finally peer-reviewed and published in @springernature.com Experimental & Molecular Medicine (EMM)🥳: www.nature.com/articles/s12... Our story was published back-to-back with the intriguing paper by Kim et al.: www.nature.com/articles/s12...

I envy the glycoscience community for their #glycotime hashtag. A great way to make posts visible. Anyone in #ChemicalProteomics that has a good idea, which hashtag we might use to make all of our work more visible and findable? #ChemSky #ChemBio #DrugDiscovery #ABPP www.nature.com/articles/s41...

The economics for TMT aren't great right now: "...first half revenue from the TMT license with Thermo Scientific falling to £0.79m (2024: £1.85m)...a stronger Q3 order for TMTpro...we still anticipate that full year revenues are likely to fall short of the 2024" files.aptuitivcdn.com/JxEdylMPMz-1...

“CSIRO has announced it will slash up to 350 jobs as the national science agency grapples with long-term financial challenges … with current funding failing to keep pace with the rising costs of running a modern science agency.” Not “challenges”. They’re choices. 😡

Sciex's new MS Data Converter 2.0 was released a month ago, as a way to generate mzML files. You can centroid a few different ways: - Intensity sum above 50% - Area - Height - Intensity - Total intensity sum (default) Anyone have any preferences? I like the default but maybe I'm missing something.

Big day for foundational glycoRNA research, with two preprints published in Nature EMM. Both urge greater analytical rigour in the field. 1. RNA-associated glycoconjugates highlight potential ambiguities in glycoRNA analysis 2. Proteins are a source of glycans found in preparations of glycoRNA

Detecting Human Milk Oligosaccharides Through Enzymatic Replacement with Azido Fucose #glycotime #Glycobiology

New @angewandtechemie.bsky.social paper jointly supervised by Zeshi Li @lizeshi.bsky.social and Geert-Jan Boons describes the chemoenzymatic synthesis of O-acetylated GD3 as intact glycolipids, and the use there of to study virus binding in cellular context. onlinelibrary.wiley.com/doi/10.1002/...

Super exiting! What could be better than winning an award? Winning in the same year as a former UW trainee (Nate), one of my closest colleagues (Bill), and two good friends (Neil and Lingjun). See you all in St. Louis!

In case you missed the live #THEProteomicsShow with me and Ben Orsburn @proteomicsnews.bsky.social, it was pretty fun. HUGE thanks to the organizers of #HUPO2025 for inviting us, and for Ken Weke (Univ. of Victoria) for being our guest. Definitely check it out wherever you get podcasts, or here:

Excited to share our laboratory's research tools to study Siglecs along with some interesting discoveries with these tools. Lot of help from many group, but @zeinab-jc.bsky.social was the major driver of this work. Please reach out if you would like to try them out. www.science.org/doi/10.1126/...

Brief observations: ASMS Meeting: 🍺→ 👩‍👩‍👦‍👦👨‍👩‍👦‍👦👨‍👨‍👧‍👧👩‍👩‍👦‍👦, always questions/proposals. HUPO General Assembly of Members: No 🍺→ 🪑🪑🙍🪑, no questions.

Wow what a fun mass spectrometer! Our preprint peaks with an MS4 experiment on oligos in negative mode, combining three different ion isolation modes and three different activation methods: electrons for EDD, collisions, and IRMPD 😅 www.biorxiv.org/content/10.1...

Congratulations to Bill Noble on receiving the 2026 Gil Omenn Computational Proteomics Award from US HUPO! us-hupo.org/Computationa...

Congratulations to Mike MacCoss on receiving the Donald F. Hunt Distinguished Contribution in Proteomics Award from US HUPO! us-hupo.org/Distinguishe...

Scientists operate on principles of good faith. But this is just ridiculous. If you, dear reader, take nothing else away from The Drain paper, it is that publishers cannot be invited to the table any longer. They can participate and carve a space, but they can't be part of shaping policy. 3/n

Questionably fun fact: The study of endotoxin in modern age was kicked off by Peter Panum (studying putrid fluids). He had also done work on albumen, releasing “something” with dilute acid hydrolysis. Eichwald in 1865 did that same reaction on mucin, arguably starting the study of glycoproteins.

The Importance of Denaturing Conditions for Protein and Peptide Quantification by Tryptophan Fluorescence pubs.acs.org/doi/10.... --- #proteomics #prot-paper

May Institute on Computation & Statistics for MS and Proteomics is taking great shape. Fantastic new and repeat instructors and lots of new content. We are grateful to our sponsors Fragmatics and Biognosys. Keep an eye on the website computationalproteomics.khoury.northeastern.edu

A few weeks ago I attended the Olympic Peninsula Fungi Festival. I went as someone who is interested in most everything in the world, lives in a wet, fungi filled temperate rainforest, and is mostly ignorant about fungi. Here are some things I learned:

"I have lost count of the number of times over the years that I’ve said “Huh, I didn’t think mass spec could do that”."

B4GALT1 and Wntless collaborate to block LRP5/6 translocation from #Golgi to cell surface, say Xinyang Li, Yue Hua, Xiaoqing Gan and colleagues (Fudan University): rupress.org/jcb/article/... #Biochemistry #Trafficking #CellSignaling #Wnt

To use it, it's best to search for the instrument model and work from there. Here's an example of all Orbitrap Astral (Zoom) tenders with purchase values: The $1 is likely a placeholder, but MPI may not be. These can also include LC, service contracts, etc, so approximation at best

That’s been the main goal with leaks and posting instrument prices, more informed MS purchases. The more informed the purchases are, the better value for money these funding bodies (and labs) get.

I've just come across a resource for finding MS prices beyond the US (GSA Advantage). It's called EU Tenders (ted.europa.eu) and has many recent MS prices on there. E.g. Astral Zoom with FAIMS Pro Duo is listed as $1.75 million Euro ($2 million USD) Limited info but probably includes an unlisted LC

This is due to training cut-offs, and because in human review, manuscripts are compared against the reviewers accumulated experience, which is not primarily stored in a textual format that is publicly accessible for the LLM to use. This argues against using centralised LLMs for peer review.

My personal experience, and anecdotes from friends, agreed with these observations.

Fun day out at Waters charge detection mass spectrometry (CD-MS) technology day.

New TMT Tribrid from Thermo for #ASMS2026 (public info) - IRMPD is the new UPVD - IRMPD with TMT boosts reporter intensity by up to 240% without affecting quant - New resolutions -> Down to 1k res, with an associated loss of signal, but at least it looks faster! - Improved proton transfer reaction

My student's paper on enzymatic remodelling to generate anionic glycan arrays has just been published online in @jbiolchem! Various recombinant glycosyl- or sulphotransferases were employed to prepare glycan motifs tested with various lectins. #glycotime www.jbc.org/article/S002...