A new high-resolution microscopy technique enables simultaneous visualization of up to 12 biomolecules in cancer cell nuclei, offering detailed insights into nanoscale nuclear organization. doi.org/hbzd5k

Our latest paper is online. www.nature.com/articles/s41... We expanded the speed-optimized DNA-PAINT sequences from 6 to 12 — enabling faster, higher-resolution multiplexed super-resolution imaging of up to 10 cellular targets. Here's the story. 1/n

Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort. www.biorxiv.org/cgi/content/...

Happy to share our Current Opinion review on the key challenges in accurately predicting 3D distances between chromatin segments and computing their dynamics. Thanks to @djost-physbiol.bsky.social @lucagiorgetti.bsky.social for the invitation to write this review! @shuvadipdutta.bsky.social

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence! www.nature.com/ncomms/edito... @natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

How short can a degron be? A compact, genetically encoded, 9-aa tunable degron that drives MDM2-dependent degradation enabling precise control of protein levels from yeast to human cells. Thanks to @eswarappalab.bsky.social for the opportunity to explore this in yeast. #Degron #Yeast #CellBiology 🧬

Interested in tunable protein degradation? Check out our new preprint describing a compact, portable degron derived from MTCH2: doi.org/10.64898/202... Led by Lekha EM Saubhik Som Ramon Boro Sandeep Eswarappa Great collaboration w/ Anubhav Dhar Eepsitha @syncellbiolab.bsky.social #iiscbangalore

I’m honored to be part of the next GRS Single Molecule Approaches to Biology, thanks Chairs @qmsmith.bsky.social and Alba Gomez Segalas for organizing this great event! The GRS is a wonderful opportunity for young scientists to share their exciting science and build communities. See you there!

We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...

Excited to share a new preprint from a team of researchers I am part of (funded by an NIH grant). The paper helps us understand a question I have been interested in for a while: why are bacteria so resilient? www.biorxiv.org/content/10.1... @meyerroc.bsky.social @juliebiteenlab.bsky.social

(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen! Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org! doi.org/10.1073/pnas... 👇🧵

Thank you for having me here, Mike. Big thanks to all the organizers. It was fantastic meeting. Looking forward to the next editions of #SMLMS2025

📢 Dear lovers of prokaryotic chromosomes: Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳. Join us! @biophychrom.bsky.social @danielabarilla.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social #microsky

Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫 www.eventsforce.net/biochemsoc/f... @vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social

And the story is published now!🍾 www.nature.com/articles/s41... Open access link: rdcu.be/exnOc Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏 #microsky #archaeasky

Very much looking forward for #SMLMS2025

🔬 Are you working with single-molecule super-resolution methods (SMLM, MINFLUX) and would like to meet experts in the field and discuss your work? 👉🏻 consider attending the SMLMS 2025 Meeting in Bonn 👉🏻 abstract submission is open until June 27th 👉🏻 www.smlms.org @smlm-symposium.bsky.social

Such fantastic news! I had the privilege of working with Cees — an incredibly inspiring scientist. His research truly deserves this recognition. Huge congratulations, Cees!

Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter! www.cell.com/current-biol...

Latest #CDlab paper online in @labonachip.rsc.org: pubs.rsc.org/en/content/a... Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!

Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?

In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

The kindest of gentle reminders. We have one week to go on one #PhDPosition and one #PostdocPosition in single-molecule dielectrophoresis. Do you like single molecules , advanced optics and nanotechnology? More details in the thread👇 Dear Bluesky poplulation, please help with reposting

Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1... ▶️40-fold flanking sequence effect on TF binding ▶️Motif recognition beats kinetics ▶️Minutes-long residence times ▶️Models of TF-nucleosome competiton

This is a fantastic community to be part of. Don’t miss the chance!

Amazing!

🔬 Ever wondered how therapeutic antibodies work? 3D LLS-TDI-DNA-PAINT and live-cell LLS microscopy allow decoding of the molecular interplay of endogenous CD20 on B cells with therapeutic antibodies. 📄 Read more: www.science.org/doi/10.1126/...

Really excited to share our latest preprint in collaboration with Sandeep Choubey's lab at IMSc. Mycobacterium tuberculosis Nucleoid Associated protein Lsr2 and DNA undergo sequence-dependent co-condensation. Check it out here: www.biorxiv.org/content/10.1...

From the Henriques lab comes NanoPyx, which optimizes bioimage analysis by dynamically selecting code variations based on input data and hardware to ensures maximum computational efficiency. www.nature.com/articles/s41...

Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution www.biorxiv.org/content/10.1...

Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇

Our lab has an opening for a core member of our team: a talented senior scientist fascinated by single-molecule biophysics with a focus on novel instrumentation and experimentation. See the ad here: my.corehr.com/pls/uoxrecru... Deadline for applications: February 14, 2025.

Our new paper is out in PNAS! We engineered bacteria to display enzymes that sea sponges use to mineralize their glass skeletons. These bacteria can self-assemble a bioglass coating around themselves, and focus light into bright, photonic nanojets. www.pnas.org/doi/10.1073/...

I could not be more thrilled to announce the Nature Methods @naturemethods.bsky.social Method of the Year is Spatial Proteomics! Please see our editorial as a roadmap to the fantastic content in this special issue! www.nature.com/articles/s41...

Well this is some cool single molecule imaging www.nature.com/articles/s41...

Our iGEM team designed their own screen-printed biophotovoltaic cells that can produce electricity while removing CO2, powered only by photosynthetic bacteria! Check out our pretty green cells here: www.rochester.edu/newscenter/u... @igemhq.bsky.social @urochester.bsky.social

Very interesting study on how ticks stay bound to host from my friend Siddharth Deshpande lab at Wageningen university. Phase separation! www.nature.com/articles/s41...

De novo catch bond using designer DNA structure! www.nature.com/articles/s41...

Glad to have contributed to this beautiful study!