Trussler et al. use comparative RNA-seq to explore how an sRNA derived from a transposon affects >200 genes and acts as global regulator of Salmonella invasion and flagellar genes. onlinelibrary.wiley.com/doi/10.1111/... #MicroSky #sRNA

Dong, Zou, and Cronan report the fatty acid preferences of the multiple FakB fatty acid binding proteins that function with FakB to convert exogenous fatty acids to acyl phosphates in Enterococcus faecalis. onlinelibrary.wiley.com/doi/10.1111/... #MicroSky

Fig4 is a lipid phosphatase conserved in all eukaryotes and is associated with human neurological diseases. Using S. cerevisiae, Reeves et al. explore how Fig4 disease-related mutants alter TORC1 signaling. onlinelibrary.wiley.com/doi/10.1111/... #MicroSky

RibR regulatory proteins bind to FMN-sensing riboswitches, override their genetic decisions, and stimulate riboflavin gene expression. Hübenthal and Mack show that RibR-like regulator proteins may be more widespread than anticipated. onlinelibrary.wiley.com/doi/10.1111/... #MicroSky

Zhang et al. show that adaptation of the plant pathogenic fungus Botrytis cinerea to mild heat stress requires both autophagy and the mitochondrial Lon1 protease. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Rev1, a translation synthesis DNA polymerase of Candida albicans, binds to PCNA via its BRCT domain and plays a critical role in DNA damage response, but is dispensable for systemic candidiasis. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Candida albicans is a major fungal pathogen whose virulence is linked to filamentation. Its relative, C. dubliniensis, filaments less and rarely causes disease . Meza-Davalos et al. explore the regulatory basis for this difference. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Yeh, Kelly, and Misra show that mutations in RNA polymerase (rpoB) mimic the stringent response in the absence of (p)ppGpp and help maintain a healthy proteome under extreme growth temperatures and without molecular chaperones or proteases. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Becam et al. report unexpected anaerobic phenotypes associated with CueO, a periplasmic multicopper oxidase that contributes to copper resistance. This suggests that CueO may use an alternative electron acceptor in the absence of oxygen. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Amidotransferases are essential for protein lipoylation in diverse eukaryotes. The selective inhibition by 8-bromooctanoate of amidotransferases from Capsaspora owczarzaki and Plasmodium falciparum reveals a conserved and druggable mechanism. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Diverse fungal chitin synthases affect the growth and secondary metabolism of Monascus purpureus. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Tiffany Zarrella et al. provide new insights into the role of the bacterial second messenger cyclic di-adenosine monophosphate (c-di-AMP) in maintaining cell envelope homeostasis in Streptococcus pneumoniae. #MicroSky #cyclic-di-AMP onlinelibrary.wiley.com/doi/10.1111/...

Xingru Chen et al. describe the role of the PhyK orphan kinease in Brucella obis. PhyK is a key regulator of cell envelope properties that can modulate host immune interactions. #MicroSky #BLAST onlinelibrary.wiley.com/doi/10.1111/...

Natalia de Miguel et al. present a new Review on how protozoan parasites communicate with each other and these processes affect social activities, physiological processes, and disease progression. #MicroSky #protozoa #parasitology onlinelibrary.wiley.com/doi/10.1111/...

Li et al. characterize two ArsR-type transcriptional repressors (sHdrR and SoxR) that use conserved cysteines to sense sulfate sulfur! #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Super useful - thanks all!! Just spent three hours playing with this - really nice to have that data accessible!!

Jinich et al. present an important new resource for the Mtb community! The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB) is an interactive web resource summarizing 150+ published screens. #MicroSky #Mycobacterium #tuberculosis onlinelibrary.wiley.com/doi/10.1111/...

Stevens et al. describe the molecular basis for increased antibiotic tolerance in S. aureus COL: a prs mutation that reduces PRPP synthetase activity (leading to downregulation of pyrimidine, histidine, and tryptophan synthesis) plays a major role. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Sze et al. describe the role of FliS in post-transcriptional regulatory mechanisms governing flagellar assembly in B. burgdorferi, a spirochete with periplasmic flagella. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Nice cover @molmicrounihoh.bsky.social features Blastocrithidia nonstop, a trypanosomatid parasite with a non‐canonical genetic code. Associated paper by Slava Yurchenko, Julius Lukes and colleagues describes its genetic transformation: onlinelibrary.wiley.com/doi/10.1111/...

Experimental evolution studies reveal that Candida auris acquires amphotericin B resistance by diverse mechanisms; some dependent on and others independent of sterol content modulation. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Dornes et al. show that Bacillus subtilis GpsB, a cell cycle regulator, can interact simultaneously with FlhG and PBP1. This supports a model in which a GpsB-FlhG interaction localizes flagella biosynthesis to regions of cell wall biosynthesis. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Yang and Swingle show that Pseudomonas syringae swimming motility is induced by proximity to other bacteria, leading to the formation of asymmetric colonies. This induced social behavior requires Moco. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

In Corynebacterium glutamicum, iron and heme homeostasis are controlled by the global regulators DtxR and HrrA. Here, these two overlapping regions are explored using chromatin affinity purification and sequencing (ChAP-Seq). #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Hill et al. use the Pseudomonas syringae ice nucleation protein (INP) surface display system to present Borrelia burgdorferi lipoproteins on the surface of E. coli to reveal conservation of complement inhibitory functions. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

During nitrogen fixation, the methanogen Methanosarcina acetivorans produces the alternative vanadium-nitrogenase and iron-nitrogenase when molybdenum is unavailable to support the activity of molybdenum-nitrogenase. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

5-oxoproline (OP) a toxic by-product of metabolism generated by all organisms. McBride and colleagues demonstrate that the Clostridioides difficile PxpAGBC system detoxifies OP and allows OP to be used as a nutrient source. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

PldX, a luminal Phospholipase D, is necessary for the efficient destruction of ingested Klebsiella pneumoniae bacteria in Dictyostelium discoideum phagosomes. PldX and the AlyL lysozyme together attack the bacterial membrane and permeabilize it. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Using bioinformatic tools, the authors identify a diverse defensome in the animal pathogen Actinobacillus pleuropneumoniae, featuring various defense mechanisms including CRISPR, Abi, and restriction-modification (RM) systems. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

If you are interested in publishing with Molecular Microbiology check to see if you may be eligible for institutional or funder support! Article Publication Charges (APCs) may be covered by a Wiley Open Access Account. authorservices.wiley.com/author-resou...

Veronica Godoy and coworkers identify a TetR-family regulator of the umuDC and umuC genes in A. baumannii, thereby controlling error-prone DNA synthesis (EppR). #MicroSky #replication #mutation onlinelibrary.wiley.com/doi/10.1111/...

Matavacas & von Wachenfeldt demonstrate that disruption of protein homeostasis affects B. subtilis biofilms -- dnaK mutants have a reduced motile population and more matrix producers and endospores. onlinelibrary.wiley.com/doi/10.1111/... #MicroSky

New RESOURCE article: Yurchenko et al. introduce powerful genetic tools for Blastocrithidia Nonstop, a protist with an unusual genetic code: all three standard stop codons encode amino acids, with UAA also serving as sole stop codon. #Microsky #parasitology onlinelibrary.wiley.com/doi/10.1111/...

The C-terminal domain of Porphyromonas gingivalis type IX secretion system (T9SS) cargo protein (PorA) plays a dual role: as a secretion signal directing the secretion of PorA and as a positive regulator of T9SS. #MicroSky #ECFsigma #T9SS onlinelibrary.wiley.com/doi/10.1111/...

New Review: Florence Hommais and coworkers explore how small RNAs (sRNAs) regulate nucleoid-associated proteins (NAPs), how NAPs regulate sRNAs, as well as the role of sRNAs in nucleoid structuring. #MicroSky #Chromatin #sRNA onlinelibrary.wiley.com/doi/10.1111/...

Competent streptococci release hydrolases associated with fratricide (bacteria kill closely related cells to release DNA). The ScrI protein substitutes Thr for Ala in peptidoglycan cross bridges to confer immunity! #MicroSky #Peptidoglycan #Competence onlinelibrary.wiley.com/doi/10.1111/...

Isabelle Coppens and coworkers investigate the role of a Golgi reassembly and stacking protein (GRASP) in the proliferation and virulence of Plasmodium berghei in hepatocytes. #MicroSky #parasitology #Plasmodium onlinelibrary.wiley.com/doi/10.1111/...

Consider *Molecular Microbiology* for your next submission! To read more re: journal scope and additional information: onlinelibrary.wiley.com/journal/1365... Authors can reach out to any of our nine academic editors with pre-submission inquiries! onlinelibrary.wiley.com/page/journal... #MicroSky

Barreto and Fowler explore how the widely conserved PhoPQ TCS evolved in Salmonella enterica to adopt a new role as a master regulator of gene expression within its species-specific intracellular niche: the Salmonella-containing vacuole. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Nice to see our review with Ramses Gallegos-Monterrosa among the top 10 cited papers of 2023 at @molecularmicro.bsky.social 🎉

New insights into the enzymology of the Na+/H+-exchanging ATPase, PfATP4, from the malaria parasite Plasmodium falciparum. This enzyme is an attractive drug target with one compound (the spiroindolone cipargamin) in clinical trials. #MicroSky #malaria onlinelibrary.wiley.com/doi/10.1111/...

How rapidly do oxygen & ROS cross biological membranes and what does this mean for their detoxification? Jim Imlay tackles this question in a new Perspective, which provides a quantitative framework for thinking about how gases interact with cells. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Simon et al. explore the the Roc (regulation of cup) two-component systems in P. aeruginosa, including two histidine kinases (RocS1 and RocS2) and (now) 5 response regulators that regulate virulence, antibiotic resistance, and biofilm formation. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Perspective. MecA: A Multifunctional ClpP-Dependent and Independent Regulator in Gram-Positive Bacteria... from Zezhang T. Wen et al. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Chromatin aficionados take note: Second of two special issues on Prokaryotic Chromosome Organization now published! (6 review/perspective articles and 2 research articles) Guest edited by @damelab.bsky.social #MicroSky #Chromatin onlinelibrary.wiley.com/toc/13652958...

A human microRNA induces cytoskeleton modifications through RhoA activation to increase dissemination of Shigella flexneri in the host! New work from Laurence Arbibe and coworkers #MicroSky #cytoskeleton #Shigella onlinelibrary.wiley.com/doi/10.1111/...

In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...

First of two special issues on Prokaryotic Chromosome Organization now published! Guest edited by @damelab.bsky.social #MicroSky #Chromatin onlinelibrary.wiley.com/toc/13652958...

New review from Lisa R. Racki and Lydia Freddolino: Polyphosphate: The “Dark Matter” of Bacterial Chromatin Structure! #MicroSky onlinelibrary.wiley.com/doi/10.1111/...

Now on EarlyView! NrdR regulates the expression of ribonucleotide reductase. Inna Rozman Grinberg et al. reveal a conserved mechanism of NrdR regulation, providing insights for predicting NrdR binding sites and developing novel antibiotics. #MicroSky onlinelibrary.wiley.com/doi/10.1111/...