Vaguely remember differences in glycosylation for spike alone vs native virus, and another paper that implicated N in slowing Golgi transit. Anyone with a better memory than me remember the refs? No doubt that viral protein effects on endomembranes are complex and overlapping.

This is a cool dive into AF3, with a really nice way to get AF3 to do something much more closely matching reality.

This might be be a dumb question, but do we know which glycans end up on the surfaces here after GGTA1/B4GALNT2/neuGc knockouts? Would obviously guess we miss these epitopes, but are they truncated, or replaced with some other epitope (apart from the obvious NeuAc)? www.nature.com/articles/s41...

A cryptic pocket allosterically modulates oligosaccharide binding to DC-SIGN www.biorxiv.org/content/10.1101/2025.08.19.671048v1 #cryoem

Sad, but probably inevitable news from Vodafone AU. I have been sitting on an ancient mobile phone plan for the past 25 years, with no minimum cost for infrequent usage, and they’re finally removing the plan. Are there any reputable places to port Australian mobile numbers to for e.g., VOIP?

Check the great commentary on @natcomms.nature.com from Trayambak Basak and colleagues about our work on #collagen GLT25D1 and the paper from Cao et al. about the LH3/GLT21D1 complex! #glycotime www.nature.com/articles/s41...

Kim Jong-un inspects the latest in North Korean shark tank technology.

If anyone is keen on testing the reglycosylation plugin for PyMOL, please get in touch! There’s a few rough edges I’d like to iron out, and would appreciate feedback.

This Lorenz Oken guy might just be on to something. The article mixes slime and mucus interchangeably, but it is a good read about the relative rarity of complex life. www.asimov.press/p/slime

CCG (incl @manno.se and @xylobecs.bsky.social) representing at the half marathon across the Øresund bridge today. Lovely weather for it!

Well, it turns out that you can actually make PyMOL do quite a lot of stuff. Here's the full workflow, integrating the amazing ReGlyco into PyMOL to re-glycosylate the LDLA linker. There's some serious magic needed to get it running as fast as it is within PyMOL. @elisafadda.bsky.social

Glycosylation of Structured Protein Domains in Cell-Free Reaction Environments | ACS Synthetic Biology pubs.acs.org/doi/10.1021/...

Interesting thread below! One thing I have been trying to understand is how NNs can store so much info in such a relatively “small” parameter space, such that the concepts are stored in the “direction” in a latent space. This idea would fit nicely with this model.

Looks intriguing! granta.com/slime/

This looks like a great resource to gain a conceptual understanding of deep learning models. It would be amazing to have a “Design Patterns” style book a la Gang of Four, just with patterns to detect, and the method to train it.

Systematic and comprehensive analysis of major localizations of alpha-dystroglycan-specific modifying enzymes #glycotime #Glycobiology

Human coronavirus HKU1 spike structures reveal the basis for sialoglycan specificity and carbohydrate-promoted conformational changes pubmed.ncbi.nlm.nih.gov/40324974/ #cryoem

A little bit of glycan binding structure for your Tuesday.

Obviously, bad. But doubly so because I would actually be really interested in seeing a galectin / Tim-3 interaction structure!

Looks like great stuff from Martin & co in thread this comment is on - but the comment highlights what could be most interesting here. Is this hinting (natural) protein space is bounded, and so if you can’t find function in extant proteins, maybe proteins simply can’t do it?

Putting together teaching material for a Medical Data Understanding course, and while looking up early medical informatics, I found out about this absolute legend. Apart from initiating what is now MEDLINE (Index Medicus), he also had an assistant, Hollerith…

I have this script I use to munge citations in word docs, that spelunks into the XML and creates Zotero/Mendeley compatible field codes. I figured it couldn’t be that hard to get it working for EndNote. It was. Even Gemini told me not to, which made me even more determined. Now it works.

And here the question: are they both active? which one is the responsible for the GalT activity? We combined #mutagenesis with #biophysics and #moleculardynamics to show that only the GT2 domain exhibits catalytic activity, facilitated by an unusual Glu-Asp-Asp motif critical for Mn²⁺ binding.

Super happy to share the final shape of our work describing the molecular #structure and #enzymatic mechanism of human #collagen #galactosyltransferase #GLT25D1 published in @natcomms.nature.com. Want to know how collagen become sweet? read the 🧵 below!

It’s not my day-job to look after HomeAssistant, but as allergy season is kicking in, I thought I should update our dashboard for allergens. This time, I tried using Aider + Gemini Pro 2.5 to do the update. Unbelievably cool workflow with reasonable code generated. github.com/hirenj/Polle...

Fascinating innate immune evasion strategy in SCIENCE #glycotime 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 𝘴𝘺𝘳𝘪𝘯𝘨𝘢𝘦 makes GLYCOSIRIN, a novel iminosugar that blocks plant glycosidases from degrading bacterial flagellin—hiding pathogens from plant immune sensors. New cryo-EM insights reveal exactly how this stealthy trick works

University leadership explaining how their administration plan works. www.quantamagazine.org/why-everythi...

My innate immune system is here for chewing gum and binding viral glycans, and I’m all out of specific lectins. www.cell.com/molecular-th...

Cool stuff. I wanted to say this is imaging of glyco process, but it is more like cellulose fibril assembly. I wonder, have we ever imaged the process of glycosylation directly? Like track donor + substrate and say when/where product appears? www.science.org/doi/10.1126/...

Hi all - if there are people here who feel they are missing from the Glyco starter pack, or the big list, please let me know! I haven’t kept up with tracking new glyco people on Bluesky recently!

Society for Glycobiology wishes to speak. www.glycobiology.org/index.php?op...

"Your scientists were so preoccupied with whether or not they could that they didn't stop to think if they should" @elisafadda.bsky.social , you know we could make an awesome plugin.

I’m as against the AI slop it’s all too easy to make, but it’s still pretty impressive tech. I always test the latest stuff on my standard prompt - riffing on Harrison Ford in “Extraordinary Measures”.

Early start to head down to GRC today. Really looking forward to catching up with the stellar cast of participants and speakers there to talk about everyone’s favourite PTM.

Just out by Shelley Jager: rdcu.be/edEnO. One of the fastest & deepest plasma glycoproteomics studies. Take home message; we even detect glycosylated cytokines and chemokines, but also still need better software. #glycotime @utrechtuniversity.bsky.social @bijvoet-centre.bsky.social

I am beginning to think that the killer app in scientific AI will be a bot that chases up admin to ensure tasks are done. All for effectivity increases in admin, but if they are poorly resourced, and your job now becomes admin herder, we’ve now moved admin tasks from parallel to synchronous blocking

There’s probably a great project in here to tease out the relationships between macrophages, β cell development, and the glycans that somehow modulate this. www.science.org/doi/10.1126/...

Hey unix/Mac nerds - does anyone have an opinion on proxying pbcopy/pbpaste through a unix socket and netcat? I have Macgyvered an entire system for sharing clipboard to consoles and repls (devcontainers, rstudio), even over SSH. I guess as long as permissions are fine on socket, no security issue?

We are recruiting for a doctoral candidate for the Mac4Me network mac4me.eu formal advert soon but please forward to your networks. The project will focus on structural and functional analysis of GAGs in ageing and cancer. EU mobility rules apply

Behind Molecule of the Month This month's feature on H5 Hemagglutinin includes a structure studied by PDBe Biocurator Deborah Harrus Learn more at PDB-101: pdb101.rcsb.org/news...

We are very happy to present our work on N-glycoproteomics!🍬 Our method enables the selective enrichment and precise quantification of intact N-glycopeptides to explore the dynamics of glycosylation microheterogeneity. www.nature.com/articles/s41...

This is a great topic, so am interested to read this review from @cmw12.bsky.social and colleagues.

GALNT5 (polypeptide N-acetylgalactosaminyltransferase 5) promotes migration and invasion of pancreatic ductal adenocarcinoma cells by activating Erk signaling pathway tinyurl.com/3cd4pz5u #glycotime

Interesting with the ST8SIA4. When we looked at expression across cell types, we suggested this as a “workhorse” gene, expressed in most cells. Kind of interesting that it is not exp level alone controlling this (in these cells). What are the other factors? doi.org/10.1016/j.is...

Is anyone out here familiar with fully homomorphic encryption, whether it can be used for healthcare records (or register data), and even if it is feasible to do?

My daughter is learning the art of text messaging, in a bilingual manner. Sometimes messages don’t quite come across in the way she expected.

It’s #glycotime Fucosyltransferases FUT10 and FUT11 are protein O-fucosyltransferases (POFUTs) modifying proteins with elastin microfibril interface (EMI) domains. Curious to know the function(s) of O-fucosylating EMI domains www.nature.com/articles/s41...

I’m looking for a postdoc with experience in #glycosyltransferases and/or yeast/insect #proteinexpression A new EU pathfinder project begins on 1st February 2025. Contact me if interested!

CryoEM of a glycofibril - as far as I can see, it's a P[TS]XX repeat protein, where the proline is actually a diHyp, enabling maximal loading of glycans on it. #glycotime. cc @benschulz.bsky.social and @elisafadda.bsky.social for spaghetti action.