Our dispatch: https://www.sciencedirect.com/science/article/pii/S0960982225014691 on the recent Hydra volume EM paper by Zhang, Rafa Yuste and colleagues: #connectomics, without synapses https://www.sciencedirect.com/science/article/pii/S0960982225013090 #neuroscience

A paper from my PhD lab is on Science! This is a really cool new way to utilize EM connectome datasets. Congrats to the team! www.science.org/doi/10.1126/...

How do neural circuits generate the walking rhythm? Using connectome simulations, @sarahpugly.bsky.social found a minimal central pattern generator (CPG) that produces oscillations in leg motor neurons. Same circuit motif for each 🪰 leg. w @bingbrunton.bsky.social www.biorxiv.org/content/10.1...

Last night Adam Kampff, the glue, the light, the catalyst, the builder, the smile, left us. He and his work transformed the lives of many labs, scientists, students. He inspired and was generous to his last transformation, working tirelessly to set up a foundation to continue the work.Thank you!

Our Method of the Year 2025 is...drumroll please...EM-based connectomics!! Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬 www.nature.com/articles/s41...

Connectomics is Method of the Year 2025!! @natmethods.nature.com just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...

Connectomics is method of the year 2025: www.nature.com/articles/s41...

An optimized X-ray nanotomography method offers an attractive alternative to volume EM for connectomics. @crick.ac.uk @psich.bsky.social psich.bsky.social @adrianawanner.bsky.social @andreas-t-schaefer.bsky.social @ucl.ac.uk @uclnpp.bsky.social www.nature.com/articles/s41...

Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41... This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)

#paper! X-rays can resolve #ultrastructure in tissues non-destructively. www.nature.com/articles/s41... Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.

Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...

For a decade, my white whale as a neuroscientist has been working to convince public+private funders to "go big" on post-mortem human tissue analysis, so we can define circuit disruptions in disease and just maybe link them with molecular drivers. This remains as tough a sell today as 10 years ago.

Enthusiastic about connectomics and X-rays? Several positions in our group at Crick for postdocs and senior scientists (permanent!). DM me if interested – and let’s talk if you are at Sfn next days or at the NIH BRAIN CONNECTS meeting today! Please share!

Excited to share my most recent postdoctoral work in the Jeanne lab @yaleneuro.bsky.social ! “Sensory processing reformats odor coding around valence and dynamics” www.biorxiv.org/content/10.1... We ask: how is a sensory code transformed across multiple stages of processing to inform behavior?

I am happy to share that the main story of my PhD is now available as a preprint! www.biorxiv.org/content/10.1... We analyses over 8500 neurons in a songbird basal ganglia dataset. To explore this and two other stories more interactively, also check out our website! www.songbird-connectomics.org

So proud of my new lab! Check out the recent songbird basal ganglia connectome - great job @alexandrarother.bsky.social !! @jmrko.bsky.social !! songbird-connectomics.org

Thank you so much @wellcometrust.bsky.social for supporting us to push the the limits of #X-ray #nanoimaging and #connectomics, and to make technology more broadly available to the scientific community! 🙏

Excited to try this! The waiting list makes me even more curious

The Crick is an absolutely amazing place to work in - please share this opportunity widely with your colleagues and postdocs - and DM me if I can help in any way

Apply now!!! 🔥

New blog post, where I review some recent interesting papers on the role of cell organelles for neuronal calcium signaling: gcamp6f.com/2025/08/11/i... Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!

Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context https://www.biorxiv.org/content/10.1101/2025.09.26.678648v1

Great opportunity to work with an amazing team on the frontiers of X-ray connectomics www.psi.ch/en/hr/job-op...

#Nanoimaging symposium @crick.ac.uk! At #CrickXrays25 we'll tackle: 💫synchrotron upgrades 📈current limits in throughput, volume, resolution 🧪sample preparation for X-ray PC imaging 🧠♥️applications in neuroscience and tissue life science 🗓️13th October 2025 🇬🇧🌐 London & online tinyurl.com/crickxrays25

Nature research paper: Supervised learning in DNA neural networks go.nature.com/4mOb9lH

Highly recommended

It was a good experience to step back and briefly take stock of the amazing progress in connectomics since I started working on this stuff (20 years ago!) thanks as well to @natrevneuro.nature.com for the constructive editorial interactions.

Multiplexed Neuromodulatory-Type-Annotated EM-Reconstruction of Larval Zebrafish https://www.biorxiv.org/content/10.1101/2025.06.12.659365v1

New preprint from @darbly.bsky.social‬ @briandepasquale.bsky.social‬ and my labs! If you love (or hate) mosquitoes, have a look: doi.org/10.1101/2025... We used TEM to describe the circuitry used for CO2 detection by mosquitoes. As usual with mosquitoes, nothing is as expected! 🌬️🦟

The first full central nervous system connectome dataset of an adult fly, with the most comprehensive annotation of sensory and motor neurons to date. A heroic effort with contributions from many groups, a product of the collaborative spirit of the Drosophila neuroscience community.

Happy to share our publication - Comparative connectomics of two distantly related nematode species reveals patterns of nervous system evolution www.science.org/doi/10.1126/...

Fantastic to see this collaborative work with Hopi Hoekstra out in Nature (tinyurl.com/3at3zvby). Lead by @felixbaier.bsky.social and @katjareinhard.bsky.social. Not possible without @bramnuttin.bsky.social , Chen Liu and @arnausd.bsky.social. Excited to see where this work leads.

Excited to share a connectomics dataset of hippocampus CA3. Explore the dataset: pyr.ai Paper introducing the dataset and some of our discoveries: www.biorxiv.org/content/10.1...

Huge congratulations to Kedar Narayan who received the RMS Alan Agar Award for #ElectronMicroscopy from Paul Verkade this afternoon at #mmc2025 👏👏👏🔬🙌🥇 @rmsearlycareer.bsky.social @yorkbioimaging.bsky.social

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

If you want to learn the latest developments in volumeEM from the experts, with hands-on experience on our instruments @embl.org, this course is for you! 🔬

Introducing warpfield, an open source Python library for GPU-accelerated non-rigid 3D registration. Warps and aligns gigavoxel volumes within seconds (not hours). For 3D microscopy, region-to-region and cell-to-cell matching. A collaboration with @mh123.bsky.social 🚀 github.com/danionella/w...

Linking function and structure at scale with X-rays - and several times over. Massive congratulations to the team ‪@yuxinzhang.bsky.social‬, @carlesbosch.bsky.social, @apacureanu.bsky.social @esrf.fr, @crick.ac.uk and all our collaborators.

#preprint: We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography. doi.org/10.1101/2025... @yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social @crick.ac.uk @esrf.fr

I am honored and excited to announce that I will be starting as group leader at @dandrite.bsky.social and associate professor at @au.dk in August 2025. I look forward very much to joining my future colleagues in Aarhus in doing excellent Neuroscience, with many scientific puzzles ahead to solve!

I am very happy and thankful for having been part of this amazing journey. Enjoy exploring this incredibly rich dataset!

Here at Princeton, we’re amplifying our university president’s leadership. It’s time for everyone - faculty, students, staff - to speak out. tl;dr: rights can’t be blackmailed. From me and science colleagues: www.dailyprincetonian.com/article/2025...

Synaptic architecture of a memory engram in the mouse hippocampus www.science.org/doi/10.1126/...

We are proud to present our new preprint “Correlative light and electron microscopy reveals the fine circuit structure underlying evidence accumulation in larval zebrafish”, just posted on bioRxiv (www.biorxiv.org/content/10.1...). (1/10)

How much analgesia is needed to manage pain levels in mice after brain surgeries? A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...

Together with cSAXS, the Coherent X-ray Scattering Group at PSI, we are looking for a Postdoctoral Fellow on High-Throughput X-ray Ptychographic Imaging of 🧠: psi.ch/en/hr/job-op... + work at 4th gen synchrotron + interdisciplinary team science + Swiss salary and benefits

This excellent article by Jessica Plüss explains how the on-going attacks on NIH affect biomedical research and science well beyond the US: www.swissinfo.ch/eng/science/...

The revised version of our #Platynereis #connectome paper is now out: https://elifesciences.org/reviewed-preprints/97964 Cell-type-level annotation of the whole organisms, including synaptic and desmosomal connectomes. Can be explored with CATMAID here […] [Original post on biologists.social]

fantastic work!

X-ray nanotomography can be your new friend for neuronal imaging. In our new #preprint (tinyurl.com/nanoXneuro) we present advances that push the spatial resolution limit for X-ray holographic nanotomography (XNH). We can now resolve synapses. 🧵