How did I not know about this super-useful site for customized 3D-printable thread adapters? HT to @leon-kremers.bsky.social : brainright.com/adapt-o-matic/

The long wait is finally over: The @thorlabs.bsky.social 8 kHz resonant scanner is available 🔬! It would be lovely if there would be a 30 mm cube compatible & acoustically shielded mount as well, but I'm sure that's going to come eventually! #Microscopy www.thorlabs.com/newgrouppage...

Understanding behavior requires datasets that capture humans while carrying out complex tasks. The kitchen is an excellent environment for assessing human motor and cognitive function, as many complex actions are naturally exhibited in kitchens from chopping to 🧽! arxiv.org/abs/2506.01608

« Diverse calcium dynamics underlie place field formation in hippocampal CA1 pyramidal cells. » A fundamental study now published @elife.bsky.social elifesciences.org/reviewed-pre...

New blog post about a fun project where I tried to beautify my office by putting a poster of the hippocampal column onto my walls: gcamp6f.com/2025/08/13/d... EM pictures from this paper: www.biorxiv.org/content/10.1... from the lab of @helenelab.bsky.social

New blog post, where I review some recent interesting papers on the role of cell organelles for neuronal calcium signaling: gcamp6f.com/2025/08/11/i... Featuring work by @lorenabenedetti.bsky.social, O'Hare et al. and Lin et al.!

#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪 A sensitive orange fluorescent calcium ion indicator for imaging neural activity www.biorxiv.org/content/10.1...

New blog post with a review of interesting papers on VIP interneurons in cortex and hippocampus: gcamp6f.com/2025/06/23/i... Covering work from @koenvervaeke.bsky.social's lab with @mateneubrandt.bsky.social, Yoav Adam's lab and Bernardo Rudy's lab.

#neuroskyence folks: as my postdoc grant is running out soon, I am looking for new opportunities in systems neuroscience! Keywords: patch clamp ephys, opto, mouse behavior, (in vivo) voltage imaging. Would love to return to the Basal Ganglia. Sharing appreciated, and happy #FluorescenceFriday !

It is out! Read how you achieve 1.7 mm deep 3-photon imaging in prefrontal cortex with dendritic spine resolution over a week. www.nature.com/articles/s42...

1/6 Why does the brain maintain such precise excitatory-inhibitory balance? Our new preprint explores a provocative idea: Small, targeted deviations from this balance may serve a purpose: to encode local error signals for learning. www.biorxiv.org/content/10.1... led by @jrbch.bsky.social

The culmination of years of hard work by Anna Maria Reuss & Co: aDISCO - a protocol for clearing & antibody-staining human formalin-fixed paraffin-embedded (FFPE) tissue! With lots of @mesospim.bsky.social #lightsheet #Microscopy @ptrrupprecht.bsky.social & many more! www.biorxiv.org/content/10.1...

Introducing warpfield, an open source Python library for GPU-accelerated non-rigid 3D registration. Warps and aligns gigavoxel volumes within seconds (not hours). For 3D microscopy, region-to-region and cell-to-cell matching. A collaboration with @mh123.bsky.social 🚀 github.com/danionella/w...

Our study on spike inference from calcium imaging data in spinal cord is now published at the Journal of Neuroscience @sfnjournals.bsky.social ! 🥳With open datasets and public reviews: www.jneurosci.org/content/45/1...

Super happy that our papers #2 and #3 from our 3-person lab just went online on bioRxiv! First large-scale 3D EM dataset from mouse hippocampus CA1 www.biorxiv.org/content/10.1... - check out the beautiful data on webknossos wklink.org/7023 ! …

The BlueSpot Talk is a seminar series about the locus coeruleus organized by David Weinshenker. Today at 11 AM EST or 5 PM CET, I'll give a talk: "Effects of locus coeruleus on hippocampal astrocytes and neurons during natural and artificial arousal" zoom.us/j/99332227402

New blog post on online spike inference from calcium imaging data with deep learning: gcamp6f.com/2025/03/24/o...

That was a fun project! Thanks to Rob Lees, Adam Packer, and all the co-authors for inviting me to participate. rdcu.be/edQXi

Question for my neurobiology colleagues: what (if any) information do second messengers in neurons carry about their specific synapse of origin? In other words, if I were to measure only molecular signals in the soma, to what extent could I resolve a specific pattern of synaptic activation?

New blog post: Non-linearity of calcium indicators: history-dependence of spike reporting gcamp6f.com/2025/03/14/n..., highlighting an interesting analysis of our recent preprint.

Technically very interesting new paper from Na Ji's lab using an upgrade of their FACED technology for ultrafast (~1000 fps) two-photon scanning for voltage and calcium imaging: www.biorxiv.org/content/10.1...

Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1... - Calcium imaging - GCaMP8 vs. GCaMP6 - Spike inference - Imaging + ephys ground truth With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.

How much analgesia is needed to manage pain levels in mice after brain surgeries? A single dose of meloxicam seems to be enough. Even the addition of opioids cannot provide a clear benefit. (in line with recent work from others, see cites in our preprint): www.biorxiv.org/content/10.1...

I am very proud to announce that my PhD paper finally came out in Cell! In this *very* collaborative study, we develop and release a deep-learning approach to predict neuron type identity from their electrical signature. doi.org/10.1016/j.ce... 1/16 🧵

New blog post on how to use Google Scholar as a scientist: - Create a Google Scholar profile - Curate your publications - Annotate shared first authorships - Follow related research gcamp6f.com/2025/02/18/h...

How to guide a micropipette into mouse hippocampus using two-photon microscopy - great protocol paper by Kevin Gonzalez and the Losonczy Lab: www.nature.com/articles/s41...

Apparently, Scientifica has discontinued the sale of multiphoton microscopes: www.scientifica.uk.com/news/strateg...

In @elife.bsky.social: Multimodal mismatch responses in mouse auditory cortex doi.org/10.7554/eLif...

A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo. It works well with wide-field imaging, two-photon microscopy, and fiber photometry biorxiv.org/content/10.1...

Absolute measurement of fast and slow neuronal signals with fluorescence lifetime photometry at high temporal resolution by Bernardo Sabatini and team: www.biorxiv.org/content/10.1...

For all you place cell aficionados: there is a cool new preprint from my colleagues @mateneubrandt.bsky.social, @noralenkey.bsky.social and @koenvervaeke.bsky.social, showing that VIP interneurons gate place cell plasticity in the hippocampus! www.biorxiv.org/content/10.1...

Cool experimental design by @simwieg.bsky.social's lab with optogenetics + calcium imaging: how place cells in CA1 are affected by stimulation of contralateral CA3. They observe less an induction of place fields but somewhat random shifts of existing place fields: www.biorxiv.org/content/10.1...

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

I'd like to build a map of maps in neuroscience. Who has done some aggregation work of putting together at all the existing data repositories, atlases, maps, etc. tackling electrophy, morpho, cell types, transcriptomes, connectomes (micro and meso) to figure out what we know about the brain?

Happy head direction cell day y’all! On this day, 41 years ago, Jim Ranck recorded the first “HD cell” in his Brooklyn lab in 1984. Jim shared the news with the world at SFN that year, showing a video of the cell’s firing on a TV he somehow acquired and brought to his poster… (1/3)

If you want to understand the brain, should you work as a neuroscientist in the lab? Or teach the subject for students? Or simply read textbooks and papers while having a normal job? In this blog post, I share some thoughts on this topic. gcamp6f.com/2025/01/08/a...

Our preprint reporting on a green lifetime biosensor for Ca2+ was reviewed by eLife: elifesciences.org/reviewed-pre... I'm quite happy with the 'eLife Assessment':

Let's see if bluesky = 2016NeuroTwitter: Two-Photon optophys people: Are you using a denoising step in your pipelines? If yes which?

Advent of Comp Neuro ALERT!! 🎄🤖🧠🧪 This advent season I will be posting some papers in Computational and Systems Neuro that I think are *underrated* and deserve some more attention! ✨ Stay tuned not to miss any! #neuroskyence #neuro #advent

The Ventana SL and Ventana TL are now available. pacificoptica.com/ventana/ This is a new standard in scan engine optics. Nothing else comes close. These optics enable diffraction-limited imaging over a huge field-of-view. They're easy to integrate into your systems.

Delighted to highlight our new paper in @natureportfolio.bsky.social entitled 'Synaptic basis of feature selectivity in hippocampal neurons' (thread below 👇): www.nature.com/articles/s41...

Neuroscience students asked us to teach a PRACTICAL course on experimental methods, and it is now on YouTube! Please like and repost to help us get the word out! www.youtube.com/playlist?lis... Lecture 1: Signals and data acquisition Focusing on hardware, digital/analog I/O, synchronization 🧵

High-speed 3D random access two-photon imaging at ≥ 300 kHz – with SPARCLS: opg.optica.org/optica/fullt... By the outstanding @carolineberlage.bsky.social and team, including @urslucasboehm.bsky.social, @andrewplested.bsky.social et al.

Very interesting technical paper showing that expression of AAVs is particulary strong in CA2 (and also why this is the case): www.biorxiv.org/content/10.1... We have also made this observation (off-target effect when targeting CA1), so it's nice to see this confirmed (and explained!).

How to rebuild your network on @bsky.app and improve your experience here? I made a short starter guide with resources. www.neuronaldynamics.eu/resources/bl... Also, a thread 🧵 below.

Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!

fluorescence lifetime of GCaMP, i.e. how long it takes GCaMP to emit a photon after excitation. In this paper, they make a GCaMP6s variant that is ~3x faster than the original using a fluorescent protein called BrUSLEE. This makes sense because Bruce Lee was ~3x faster than a normal human 3/

Saw today's @xkcd.com comic, realized it worked equally well for us, too! #neuroskyence #neuroscience Original: xkcd.com/2933/

Can we please bring back beautiful (raw) data visualizations on title pages and tone down the use of illustrative artwork? Also: please ban genAI from title pages.