“Because [science] is the most effective way of learning about the physical world, it erodes superstition, ignorance and prejudice, which have been at the root of the denial of human rights throughout history…” -Baron Taverne

“Because scientific knowledge is tentative and provisional, it is the enemy of dogma.” -Baron Taverne

“Because science rejects claims to truth based on authority and depends on the criticism of established ideas, it is the enemy of autocracy.” -Baron Taverne

In 2025, we unlocked new frontiers in multiphoton optics and imaging. In 2026, you can get the best 2p systems you’ve ever seen. Accelerating your work. Higher throughput. Easy to use. Focus on your neuro work, and leave the 2p to PacOp.

Happy Halloween! pacificoptica.com/cousa/

Check out some sweet Ventana Wide + Cousa imaging. pacificoptica.com/ventana/ Live raw data over a 3.2 mm wide field-of-view with 16,000 lines/s scanning and submicron resolution. Demo now at @omibs-mbl.bsky.social Thx @ewadorotaz.bsky.social @mbfbioscience.bsky.social @crisrodtsou.bsky.social !

@pacificoptica.bsky.social @leicamicrosystems.bsky.social @thorlabs.bsky.social

Scientists explore, measure, gather evidence, figure things out, and share openly. Their work benefits us all. Demo imaging at MBL/Woods Hole and CSHL this summer. Explore further. pacificoptica.com

Put whatever objective you want on the Ventana Wide. It will give you the largest field of view with high speed scanning: eg, 3.2mm x 3.2mm with a 10x. Fast, flexible 2p imaging. Che-Hang Yu is helping MBL courses with this Ventana Wide system pacificoptica.com/ventana/

Just landed in Woods Hole for summer courses: a Full Ventana Wide system. pacificoptica.com/ventana/ with a Cousa pacificoptica.com/cousa/ www.mbl.edu/education/ad... www.mbl.edu/education/ad... Thanks, Coherent! Thanks, MBF! Thanks to course leadership and MBL staff!

Widespread cortical representations of innate behaviors in the mouse. www.biorxiv.org/content/10.1... Using the Diesel2p pacificoptica.com/diesel2p/, they found that "more directed and unilateral grooming movements had greater cortical associated responses than more stereotyped bilateral movements"

We will start gathering at about 3:00.

吉祥寺 日曜日。2-photon, optics, 生ビルと　TexMex 食べ物。 maps.app.goo.gl/xoRrGNX7xvK1... ふるってご参加ください。

No more stitching artifacts. Just image the whole field-of-view, at high resolution, in one pass. The Ventana systems give submicron resolution over huge fields-of-view almost 10 square mm. At high speed. Cover a 3.2 mm x 3.2 mm area in 4k resolution in 0.26 s. pacificoptica.com/ventana/

Explore further. pacificoptica.com/diesel2p/

Cool!! This is an exciting new Neuro AI #NeurIPS competition. By the way, the large scale 2p data for this NeurIPS competition was taken with a Diesel2p system. pacificoptica.com/diesel2p/

🌌 Curious how visionary engineering shaped one of humanity’s greatest scientific instruments? Discover the architectural origins of the JWST in this in-depth #SPIE_JATIS article: www.spiedigitallibra...

ALERT: New journal club style! S. L. Smith listens to and provides commentary on an AI-generated fake podcast on their new preprint. Hilarious in parts, and fun and chill. Everyone: Please do this for your papers too! m.youtube.com/watch?v=qO1n...

So excited to hang the ‘Acknowlegement Guidelines’ poster up in Chapel Hill! Grateful to be part of the community working towards equitable recognition for imaging scientists!! Read more and download the poster (in 10 languages!): lnkd.in/eSQRugJX #CreditWhereItsDue #AcknowledgementGuidelines

Now published in Plos Biology journals.plos.org/plosbiology/...

Animals sense in complex environments, and neuronal responses to olfactory/scent stimuli can be shaped by recent history going back ~ 30 s. Here, Subramanian, Storace, et al. used the Cousa objective pacificoptica.com/cousa/ to neural activity in vivo. doi.org/10.1093/chem... #microscopymonday

Multinucleated giant cells (MNGCs) emerge as ovaries age, and are hard to study. Here, Converse, Pritchard, Duncan et al. used 2p imaging with the Cousa objective pacificoptica.com/cousa/ in their comprehensive study of age-associated MNGCs. #microscopymonday www.biorxiv.org/content/10.1...

This system has tons of fascinating tech— optics, detectors, software— so many scientists and engineers collaborated on this international project, enjoy the fruits of their talents!

Fantastic work (as always) from Che-Hang Yu and colleagues in Spencer LaVere Smith's lab. Very neat method to increase scan speed and/or FOV in 2p microscopy. Commercially available from @pacificoptica.bsky.social. I'd love to build this for a client at some point! www.biorxiv.org/content/10.1...

🚨THIS IS NOT A DRILL🚨We are 6 days away from the world's First Look at images from Rubin! #RubinFirstLook 🔭🧪 NSF–DOE Rubin Observatory will take a new 3200-megapixel image every ~40s, yielding ~20 terabytes of data every night! The deluge of data is about to begin 🌊

ALERT: New journal club style! S. L. Smith listens to and provides commentary on an AI-generated fake podcast on their new preprint. Hilarious in parts, and fun and chill. Everyone: Please do this for your papers too! m.youtube.com/watch?v=qO1n...

Riddle: When is an "optical invariant" is not so invariant? How can you get a larger field-of-view for free? No change in resolution or scan speed. No more stitching needed! For #MicroscopyMonday here's a new preprint to check out. 1/9

Scientists at work. This is a #ucsantabarbara collab with www.mbfbioscience.com Diesel2p pacificoptica.com/diesel2p/

Upgrade your 2p to 4k. We used to have to trade off resolution to get a larger field-of-view. Now, with Ventana, you don’t have to. Keep your resolution. Keep your frame rate. And get 4x the field-of-view. pacificoptica.com/ventana/

What’s new, etendue? Spencer LaVere Smith will be presenting new optics at the @optica.org conference in San Diego tomorrow.

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

Multiphoton imaging products from Pacific Optica are now easier to get in Japan. すごい！https://www.symphotony.com/manufacturers/pacificoptica/

youtu.be/9zpWEOJWuD0?... Ventana imaging. The best 2p system. Fast, high rez, 3+ mm field-of-view. pacificoptica.com/ventana/ Turnkey systems, or parts.

We’re seeking a postdoc/student excited about animal communication, brain-wide circuits, microscopy, singing fish (Danionella), wacky ideas and who likes to: get things done, experiment, analyze, ask, learn, help and cooperate. jlab.berlin

Brand new online today. The biggest fields-of-view scanned fast, for multiphoton imaging. pacificoptica.com/ventana/

New products for two-photon imaging. Read and see dpecs here: pacificoptica.com/ventana/ Huge fields of view scanned fast. Unprecedented capabilities. #photonicswest

Interested in understanding how things work? In particular the tools you use to study the brain? Join us at TENSS 2025 where we brainstorm ideas, build and debug microscopes, electrophysiology and behavior rigs amidst the picturesque Transylvanian hills! tenss.ro Apply by: February 16th!

Our fully 3D-printed microscope is featured in @newscientist.bsky.social www.newscientist.com/article/2462...

If you need a last minute stocking stuffer, how about a set of Diesel2p optics? We can ship quickly! pacificoptica.com/diesel2p/

The Ventana SL and Ventana TL are now available. pacificoptica.com/ventana/ This is a new standard in scan engine optics. Nothing else comes close. These optics enable diffraction-limited imaging over a huge field-of-view. They're easy to integrate into your systems.

Microscope users: Horses wear blinders to restrict their field-of-view (FOV). You might not be getting your full field of view either. Learn why in this thread. (1/
8) #FluorescenceFriday

A beautiful Diesel2p system. pacificoptica.com/diesel2p/ The only large field-of-view dual arm 2p. Imaging and optogenetics anywhere in 25 square mm field of view. Want one? We can ship immediately. (this rig built by www.inss.org.uk)

This is the fastest technique yet for 3D random access multiphoton. And so elegant. If you’re interested in getting it in your lab, let us know. Beautiful work, @carolineberlage.bsky.social and @benjulab.bsky.social !

High-speed 3D random access two-photon imaging at ≥ 300 kHz – with SPARCLS: opg.optica.org/optica/fullt... By the outstanding @carolineberlage.bsky.social and team, including @urslucasboehm.bsky.social, @andrewplested.bsky.social et al.

Gigantic field-of-view, through 20 mm of air. The Cousa objective. comment: www.class5photonics.com/news/unveili... paper: doi.org/10.1038/s415... product: pacificoptica.com/cousa/

Coming soon

Our Diesel2p and Cousa have been purchased by leading labs at UCSB, UCSD, UCSF, Stanford, Princeton, NIH, Harvard, Caltech, Baylor, Cornell, Salk, Yale, Albert Einstein, Johns Hopkins, U Minnesota, Columbia, NYU, and labs in France, Italy, Greece, UK, Portugal, Japan, Norway, Canada, and more.

The Cousa objective is the world's first ultra long working distance microscope objective optimized for multiphoton imaging. Offers a 20 mm working distance in air, a huge field-of-view, and submicron resolution. pacificoptica.com/cousa/

The Diesel2p is the largest field-of-view two-photon microscope. 25 square mm, resolves individual dendritic spines, and has two completely independent scan engine arms. See two places at once. Optogenetics + imaging. Customized for your science. We can ship fast. pacificoptica.com/diesel2p/