I feel like I am echoing the editorial, but the Points of View and Points of Significance series were very influential to my scientific journey. I am thrilled to see Points of View come back, being helmed by @helenajambor.bsky.social who has already done great work amongst the microscopy community

The #ThermoFisher #antibody scandal is an ugly reminder, I think, of how few people (including those at TF, it seems) are trained to properly validate antibodies before use. Some advice on antibody validation in the posting 👇 totalinternalreflectionblog.com/2022/09/18/h...

Oh and of course the editorial that was not supposed to be shared: diabetesjournals.org/care/article...

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Run controls. Always. Anything short of that isn't science. Full stop.

Update on GeneFrame alternative for bacterial imaging. After discussion with us and others, SunJin lab have how launched a substitute for timelapse imaging in geneframes www.sunjinlab.com/product/ispa... Dimensions are very similar, price is reasonable ($105 for 250) #microsky

Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog: "In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes." More: cen.acs.org/research-int...

translated: we used faked results to try and convince you to buy our over-priced antibodies, and then offer a set of lies to justify it

Check out my "rough draft" of my instrument status page for the HCBI. Any feedback before I set it up on an HCBI GitHub? hbrown227.github.io/lab-instrume...

Combining biosensor imaging data with an animated plot of the quantified intensity, based on a blog I wrote earlier: thenode.biologists.com/crafting-plo... In this version I used a dark theme

#BlueSci question. Any recommendations for an (anti-rat) NIR 2y Ab to be used with a 730 nm laser line? The 2 obvious choices are AF750 or DyeLight755, with (on paper) AF750 brighter? Not sure about comparative photostability? No appropriate JaneliaFluors? @christlet.bsky.social any thoughts?

I think it would be cool to link up all the Imaging/Analysis YouTube Channels in the "Featured Channels" section of HCBI's YouTube homepage. What are some of the best we could highlight?

Some confetti to celebrate #FluorescenceFriday

What is the public opinion on color coding treatments in figures and then using the same colors used in the graph to outline matching representative images? Is it useful or am I being too extra?

Happy #FluorescenceFriday all! Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍 Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.

Anyone (in the US) have strong opinions on what companies do the best fabric posters?

#HappyFluorescenceFriday #microscopycommunity - into Expansion Microscopy? Come to the Expansion Microscopy User Group Community Discussion Series! First session, Tue, March 10, on “Expansion Microscopy in Practice Series: Choosing the Right Protocol” Learn more: buff.ly/V4umk5E

St. Jude researchers and collaborators developed M-PACT, a tool that leverages AI to classify pediatric brain tumors using cell free DNA from cerebrospinal fluid. The approach could improve diagnosis and disease monitoring when tissue biopsy is not feasible. ow.ly/aUcU50Yh2bb

Anyone ever tried super glue (cyanoacrylate) for mounting coverslips with stained mammalian cells, to be used for fluorescence microscopy? I'm looking for ways to conserve microscopy samples for a looooooong time...

A loaded question to scream about...which agarose do you use to mix your beads to measure the PSF? Any brand #lsfm #lightsheet

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

Defending PhD student, looking over their thesis: “If I knew then what I know now, I could’ve done all of this in like 9 months.” A thread about my favorite pioneering cave explorers and why I don’t think AI will ever “solve” biology.

Want to up your microscopy and image analysis game? Applications open now for QI 2026! Join us in cold spring harbor for two weeks of intensive training in all things microscopy.

Microscopy Bat signal! I want to compile a list of biological samples that demonstrate microscope capabilities, including the reagents to generate them. Please send me anything you know of!! Beads are great for QC, but there's a different teaching benefit with biology. 1/n

Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)

Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?

Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens... -> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this? www.fishersci.com/shop/product...

Ever wondered if analyzing your images by using intensity Z-projections impacts your data? Happy to announce my guest article in Microscopy & Analysis on that topic. It's a very short read but worth for everyone dealing with image analysis! buff.ly/KJ147tI #Microscopy #ZProjection #ImageAnalysis

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

One of the most useful resources for anyone who uses fluorescence methods.

Do you like building novel optical microscopes to answer complex biological questions? How about doing that as part of one of the world’s most unique and vibrant research and education communities, which is incidentally located in a beautiful seaside New England village? 🐙🌊🔬

Not all colormaps are created equal. Notice the nonlinear brightness scaling in two very popular ImageJ LUTs! (Glow & GFB)

Another aspect to consider is how much colors are saturated, depending on the LUT, the screen type and settings it can make a huge difference on data readability. Green Fire Blue is popular but really saturated

Periodic reminder that the fluorescent compound in mammalian cell culture media, such as DMEM, is riboflavin (not phenol red)

Does anyone know a database where let say I have 2 proteins: Integrin and FAK, I can find out what motif/sequence of amino acids mediate their interaction? Thank you. #questions #academia

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager! I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

Bio-Imaging with Shakespeare: pseudocoloring (LUTs) — reveal hidden imaging issues and highlight important data. ✔ Spot noise, shading & saturation ✔ Map data for spatial insight ✔ Make color accessible to all ✔ Why to use a calibration bar Full guide, tools & tutorial inside 👇 buff.ly/8x7Shiq

#GEF25 the expansion microscopy community would benefit from high NA, long working distance water objectives. Who's working on these? What's out there?

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

Bringing some more ExM to #FluorescenceFriday with what appears to be a frustrated cell division in fibroblasts. More details in Alt-text.

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

Halfway to I2K is BACK, friends of all kinds! Last year, 650 people attended 30+ TOTALLY FREE image analysis workshops of all kinds, across many timezones. If you make image analysis software and want to teach it, workshop submissions are open now! We'd love to have your tool highlighted.

@joachimgoedhart.bsky.social and Flurescent protein colleagues--help! With advice from imaging freinds, we tagged a protein of interest with "tagRFP", which we now know from its protein sequence and digging is a derivative of eqFP578 from Entacmaea quadricolor. We want an antibody to it but 1/n

Time to add some ExM to #FluorescenceFriday ! Sample description in alt-text.

Alright I have something useful for you to do. Think of any teachers and librarians you know. Do they know about Skype a scientist? Our program matches classrooms & other groups w/scientists for virtual Q&As. It’s free! They can sign up here! www.skypeascientist.com/sign-up.html #Edusky #StemED

After seeing @miguelcmestre.bsky.social @lancasterlab.bsky.social @jamesdmanton.bsky.social 's paper this week on deep and accessible ExM imaging with cerebral organoids, I think it's a good time to repost this Starter Pack of Expansion Microscopy people! go.bsky.app/Qxks9WD

We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:

🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...