Anyone ever tried super glue (cyanoacrylate) for mounting coverslips with stained mammalian cells, to be used for fluorescence microscopy? I'm looking for ways to conserve microscopy samples for a looooooong time...

A loaded question to scream about...which agarose do you use to mix your beads to measure the PSF? Any brand #lsfm #lightsheet

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

Defending PhD student, looking over their thesis: “If I knew then what I know now, I could’ve done all of this in like 9 months.” A thread about my favorite pioneering cave explorers and why I don’t think AI will ever “solve” biology.

Want to up your microscopy and image analysis game? Applications open now for QI 2026! Join us in cold spring harbor for two weeks of intensive training in all things microscopy.

And my project on how to extend the field of depth in inverted fluorescence microscopy workflows for expanded tissues is now out out! 😁 More exciting things to come out of my PhD! 🤩

Microscopy Bat signal! I want to compile a list of biological samples that demonstrate microscope capabilities, including the reagents to generate them. Please send me anything you know of!! Beads are great for QC, but there's a different teaching benefit with biology. 1/n

Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)

Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?

Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens... -> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this? www.fishersci.com/shop/product...

Ever wondered if analyzing your images by using intensity Z-projections impacts your data? Happy to announce my guest article in Microscopy & Analysis on that topic. It's a very short read but worth for everyone dealing with image analysis! buff.ly/KJ147tI #Microscopy #ZProjection #ImageAnalysis

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

One of the most useful resources for anyone who uses fluorescence methods.

Do you like building novel optical microscopes to answer complex biological questions? How about doing that as part of one of the world’s most unique and vibrant research and education communities, which is incidentally located in a beautiful seaside New England village? 🐙🌊🔬

Not all colormaps are created equal. Notice the nonlinear brightness scaling in two very popular ImageJ LUTs! (Glow & GFB)

Another aspect to consider is how much colors are saturated, depending on the LUT, the screen type and settings it can make a huge difference on data readability. Green Fire Blue is popular but really saturated

Periodic reminder that the fluorescent compound in mammalian cell culture media, such as DMEM, is riboflavin (not phenol red)

Does anyone know a database where let say I have 2 proteins: Integrin and FAK, I can find out what motif/sequence of amino acids mediate their interaction? Thank you. #questions #academia

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager! I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

Bio-Imaging with Shakespeare: pseudocoloring (LUTs) — reveal hidden imaging issues and highlight important data. ✔ Spot noise, shading & saturation ✔ Map data for spatial insight ✔ Make color accessible to all ✔ Why to use a calibration bar Full guide, tools & tutorial inside 👇 buff.ly/8x7Shiq

#GEF25 the expansion microscopy community would benefit from high NA, long working distance water objectives. Who's working on these? What's out there?

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

Bringing some more ExM to #FluorescenceFriday with what appears to be a frustrated cell division in fibroblasts. More details in Alt-text.

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

Halfway to I2K is BACK, friends of all kinds! Last year, 650 people attended 30+ TOTALLY FREE image analysis workshops of all kinds, across many timezones. If you make image analysis software and want to teach it, workshop submissions are open now! We'd love to have your tool highlighted.

@joachimgoedhart.bsky.social and Flurescent protein colleagues--help! With advice from imaging freinds, we tagged a protein of interest with "tagRFP", which we now know from its protein sequence and digging is a derivative of eqFP578 from Entacmaea quadricolor. We want an antibody to it but 1/n

Time to add some ExM to #FluorescenceFriday ! Sample description in alt-text.

Alright I have something useful for you to do. Think of any teachers and librarians you know. Do they know about Skype a scientist? Our program matches classrooms & other groups w/scientists for virtual Q&As. It’s free! They can sign up here! www.skypeascientist.com/sign-up.html #Edusky #StemED

After seeing @miguelcmestre.bsky.social @lancasterlab.bsky.social @jamesdmanton.bsky.social 's paper this week on deep and accessible ExM imaging with cerebral organoids, I think it's a good time to repost this Starter Pack of Expansion Microscopy people! go.bsky.app/Qxks9WD

We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:

🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...

In 2019, Anna Jost & @jencwaters.bsky.social reviewed best practices for validating quantitative #microscopy methods & discuss strategies to avoid unconscious bias in imaging experiments rupress.org/jcb/article/... 📕 In #Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...

OK--fluorescent protein friends. We want an antibody to TagRFP that works in immunofluorescence. Any suggestions? Re-post please

This 2009 article by @jencwaters.bsky.social discusses the parameters of digital image acquisition that affect the accuracy & precision of quantitative fluorescence #microscopy measurements rupress.org/jcb/article/... In #Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...

We have a user that wants to set up a multipoint tiling experiment on our Nikon Spinning Disk and it will take a few hours. We need to use the Nikon water objective, which evaporates. Suggestions to stop evaporation or other immersion?

How a Hearing-Loss Grant Got Cut in the Fight Over DEI undark.org/2025/07/11/i... via @undark.org

Hello microscopists!! Help needed - does anybody know of any good Mn2+ indicators that are good in live cell microscopy assays ? Never worked with them before and not sure if there are any reliable & affordable ones out there..? Reposts appreciated 🙏🙏

Can anyone recommend a service where I upload 3D models of simple machine parts and they mail me the CNC'ed results? Something like Osh Park that does small batches but for machine parts? I feel like I saw something about this recently but can't remember which platform I saw it on.

🎀✨Recording now available ✨🎀 youtu.be/jtDunWK8g1o?...

Who would have thought inverting colors would be so tricky ? jordan.yoonbuck.com/post/contras...

I'm trying to help a high school teacher get an old Nikon E600 running for fluorescence imaging. Does anyone know if there are specs floating around for printing an adapter(s) that would allow it to take a standard LLG? She really wants to avoid having to go back to using Hg lamps.

Seeking recommendations for good price agarose for gels!! The Invitrogen Agarose I've been using for years is now $705 for 500g with internal discount, and I see no reason not to explore other options 🙈. 🧪🧬🧫

The goal is plainly and literally to cut the number of people involved in basic science in the US by 70%

We are proudly hosting 𝐃𝐫. 𝐓𝐚𝐥𝐥𝐞𝐲 𝐋𝐚𝐦𝐛𝐞𝐫𝐭 @talley.codes (CITE, Harvard Medical School): 𝐒𝐢𝐦𝐮𝐥𝐚𝐭𝐢𝐧𝐠 𝐫𝐞𝐚𝐥𝐢𝐬𝐭𝐢𝐜 𝐥𝐢𝐠𝐡𝐭 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 𝐢𝐦𝐚𝐠𝐞𝐬 𝐮𝐬𝐢𝐧𝐠 𝐦𝐢𝐜𝐫𝐨𝐬𝐢𝐦 When: 𝐌𝐚𝐲 𝟐𝟗𝐭𝐡 @ 𝟏𝟏 𝐚𝐦 𝐄𝐃𝐓 (Boston time) Join us on Zoom! harvard.zoom.us/j/9762343464...

Proud of Harvard for pushing back, exercising some creativity, and, maybe, helping folks get some needed education. Harvard is making many of their online government courses FREE. I just signed up for "Citizen Politics in America". They even asked me my Gender. pll.harvard.edu/subject/gove...

for you other colorblind coders out there, tired of missing the red X in your github actions logs .color-fg-danger, .fgColor-danger {color: magenta !important;} .octicon-x {color: magenta !important;} you're welcome

The longer we have worked on and refined this #microtubule - based model of #neurodegeneration, the more convincing it becomes. It is the fruit of 20 years of work. If you are interested, join my THE CYTOSKELETON OF NEURONS AND GLIA online talk this Thurday: ncytoskeleton.wixsite.com/webinars

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

Polypropylene. Autoclavable. Reusable. Fairly clear. One more polystyrene lab consumable eliminated? Let's find out! a.co/d/667XVjL