How does Pseudomonas putida keep its redox balance steady across different metabolic states? Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios. www.biorxiv.org/content/10.1...

Growth-coupled microbial biosynthesis of the animal pigment xanthommatin — Led by Leah Bushin, featuring M. Gracia Alvan, @danielvolke.bsky.social @oscarpuiggene.bsky.social in a fantastic collaboration w/Brad Moore @labnikel.bsky.social @natbiotech.nature.com 🦑 www.nature.com/articles/s41...

🔥🔥🔥 Friday Evening, Paper Out, Feierabend 🔥🔥🔥 When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy. doi.org/10.1016/j.ym...

🚀 Thrilled to share our new Nature Communications article: “A blueprint for designing the next-generation of synthetic C1 microbes” We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts. 🔗 www.nature.com/articles/s41... A short 🧵1/4

From the microbial networks behind kombucha fermentation to engineered strains powering sustainable fuels, #FEMSYeastRes brings you high-quality studies with major relevance across the field. 📊 Read the journal's most-read papers for the first half of 2025 buff.ly/NLKSc4N

🚨 New paper in mBio! We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10.... Here’s a teaser of how we did it 🧵👇1/5

#Synthetic C1 #metabolism in #Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway—led by J. Turlin, M. G. Alván, @oscarpuiggene.bsky.social w/@stefanodonati.bsky.social & @sebwenk.bsky.social @mbio.bsky.social #SynBio journals.asm.org/doi/10.1128/...

🌱💧 Can #bioaugmentation help crops beat drought? Discover how the #TOLERATEproject is exploring microbial solutions to boost drought resilience in EU agriculture, sustainably and at scale. 👏 Great insights by Afnan Suleiman-Bioclear earth- 🔗 Read more: tolerate-eu-project.com/how-eu-toler...

After a long sleep, environmental remediation with live biologics is back to the mainstream R&D agenda at this side of the pond. Wanna join and EU-wide on line conversation with bioremediation stakeholders this Nov 10? Express interest and register here 👉 forms.cloud.microsoft/e/b7xVz7dJcu

🌍 At #FEMSMicrobiology 2025, the TOLERATE team had the great pleasure of sharing insights from the TOLERATE project! 🧬 Our session sparked great discussions around ancient DNA and how we can revive, through synthetic biology, lost functions beneficial for future agriculture.

📍 Milan | TODAY! Join Amedea Perfumo & @pabnik.bsky.social at #FEMS 2025 for a dynamic session🧬 "Something (very) old, something new: Innovative microbial solutions for sustainability challenges 🕦 15 July | 11:30–13:00 | Tower Lounge 🔗 femsmicro.org ▶️ youtu.be/0-bhcMpjJ_c #TolerateEUProject

Preprint finally published on @cp-trendsbiotech.bsky.social! Check out the word led by @oscarpuiggene.bsky.social on methanol assimilation in P. putida via enhanced serine–threonine cycle on www.cell.com/trends/biote... Below, the original thread🧵scratching the surface of a long yet satisfying work!

Online now @ Cell is the yeast multicellular engineering paper from Fankang Meng - the fruits of his productive PhD in our group. He developed modular synthetic biology tools to bring multicellular behaviours to yeast - specific adhesion, juxtacrine signalling and more. www.cell.com/cell/fulltex...

The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇

Check out our newest article led by @oscarpuiggene.bsky.social !

🧫 @josephinegiard.bsky.social (HWU) presented #TOLERATE at the #MicrobiologySociety Early Career Conference 2025 in Newcastle 🇬🇧. Flash talk + poster led to great discussions on antifungal resistance with >10 peers & >50 attendees! 🌱 #TOLERATE #HorizonEurope #Microbiology

New phi15-based expression system in Pseudomonas putida uses #SEVA plasmids for high-yield gene expression. The work by @pabnik.bsky.social shows how single-copy vectors minimise toxicity, achieve 200×induction, and outperform T7 systems. 👉 www.nature.com/articles/s42...

Today, the #TOLERATEProject held its 1st Review Meeting (M1–M18) with PO & PM highlighting our progress, innovation & first results. A key step to shape the road ahead! 🔬🌱💦 Learn more: https://tolerate-eu-project.com/  #HorizonEurope #EUProjects #Biotechnology #SustainableAgriculture

Ethylene glycol is widely used as monomer of the plastic PET. It is therefore highly relevant as sustainable feedstock for microbial biotechnology. In our new paper in @natcomms.nature.com, we characterize efficient enzymes for ethylene glycol oxidation: www.nature.com/articles/s41...

Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍 Engaging talks, exciting science, and amazing connections — we’re full of new ideas! 🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆 🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!

Happy to host @kunjapur.bsky.social as a sabbatical #professor at DTU Biosustain, supported by Otto Mønsteds Fond! We share a passion for using #SynBio to engineer #microbes with non-natural building blocks for advanced #biotechology. He’s in #Europe — drop a line to connect & discuss cool #Science!

🎉 Excited to share that I’ve received an Emerging Investigator Grant from the Novo Nordisk Foundation! I’ll start my research group in Jan 2026 to develop universal cell factories for P2X & CO₂-based biomanufacturing 🌱🔬 👉 researchleaderprogramme.com/recipients/e...

The TOLERATE team met at DTU (Denmark) for a productive General Assembly. New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead. More info 👉 tolerate-eu-project.com Subscribe 👉 eepurl.com/i6uufM #EUProjects

What a very cool work from the @fillouxlab.bsky.social alumni Sophie Howard and @ronanmccarthy.bsky.social . Plastic degrading #Pseudomonas aeruginosa and the threat it poses in nosocomial infections and to medical devices in healthcare system. See at rb.gy/c8s6mh @cp-cellreports.bsky.social

Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc

Pathways to sustainability: a quantitative comparison of #aerobic and #anaerobic C1 bioconversion routes #methane #methanol www.sciencedirect.com/science/arti...

Excited to share a main project from my PhD, out now in @naturecomms.bsky.social! 📝 We've designed and brought to life the “CORE cycle” – a new-to-nature pathway that provides a novel route for biological CO2 capture 🦠🌱 nature.com/articles/s41... Take a look! Thread below... 🧵

Interested in engineering bacteria to turn #C1feedstocks into high-value chemicals for #bioproduction? Here is a thread summarizing our latest review on the roadblocks and how we might bio-hack our way past them 🧵 1/n academic.oup.com/femsre/advan...

Genetic dissection of cyclic di-GMP signalling in #Pseudomonas aeruginosa via systematic diguanylate cyclase disruption—led by Roman di Martino & Daniel Volke @labnikel.bsky.social @microbiotech.bsky.social #OpenAccess enviromicro-journals.onlinelibrary.wiley.com/doi/full/10....

Pseudomonas putida joins the Olympus of effective Cell Factories www.nature.com/articles/s41... Not any longer *a promising platform* for metabolic engineering, but a phenomenal, consolidated chassis for programming degradation and synthesis of amazing molecules 😃!

Congrats to Garret for officially becoming Dr. O'Connell! Garret had his Ph.D. viva on synthetic #fluorochemical #metabolism in #Pseudomonas today. Thanks to @tilmweber.bsky.social r.bsky.social, @pavelcito.bsky.social & Esteban Martinez for their insights🧪🦠 #SynBio @labnikel.bsky.social

Microbes like Cupriavidus necator naturally grow on #renewable feedstocks ♻️🧫 (CO₂, formate, hydrogen), yet their potential remains untapped due to the lack of efficient genetic tools... 1/2 www.cell.com/trends/biote...

Glad to see Julia Vorholt as winner of the @novonesis.com #Biotechnology Prize 2025 for her research on the plant #microbiome — among other achievements (e.g. synthetic C1 assimilation). Amazing and inspiring colleague #WomenInScience @eth-dbiol.bsky.social novonordiskfonden.dk/en/news/pion...

Very happy and proud of this work led by @beaubd.bsky.social with many others involved, in which we show that synthetic C1 metabolism can do better than nature for biotech!

The utilization of #C1-feedstocks, such as methanol, by non-model bacteria demands an in-depth analysis of design alternatives, a modular strategy to identify bottlenecks and efficient solutions within the host #metabolism of the chosen host... www.biorxiv.org/content/10.1...

With great collaborations come great outcomes! A fine example from @eorsi.bsky.social from @pabnik.bsky.social's lab et collaborators of how to design, build and develop cell biosensors for value-added chemicals (related to synthetic C1 assimilation, of course)! 🧪

Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint.bsky.social led by Òscar Puiggené #SynBio #Methylotrophy #CircularEconomy www.biorxiv.org/content/10.1...

Computation-aided designs enable developing auxotrophic #metabolic #sensors for wide-range glyoxylate and glycolate detection—spearheaded by Enrico Orsi w/a choral assembly of cool collaborators @naturecomms.bsky.social #SynBio #OpenAccess www.nature.com/articles/s41...

We just #published an end-to-end work for the computer-aided design💻, construction🛠️, validation✅, characterization🔬 & application💪 of #auxotroph metabolic sensors, the work-horses of in vivo growth-coupled selection screening systems-using glycolate as test case. 🧵👇1/ www.nature.com/articles/s41...

Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in Nature Communications ! 🔥 Read below to see how we developed the sensors and what they are good for 🥳. www.nature.com/articles/s41...

🚀 The SEM Group (Lab Nikel) is officially on Bluesky! We’re kicking things off with our latest paper: an end-to-end journey on designing, implementing & applying auxotrophic metabolic sensors for bioengineering! 🧬🔬 @naturecomms.bsky.social Check it out: www.nature.com/articles/s41...