🧬 Can ancient Arctic genes help today’s crops survive climate extremes? In #TOLERATE , scientists engineer root-friendly microbes with resilience traits found in ancient soils, boosting water retention, nutrient uptake & heat/drought tolerance.

Newest preprint from our lab: a new family of promising synthetic CO2 fixation cycles that may outcompete the Calvin cycle and that Vittorio Rainaldi realized to a great extent in E. coli! Up to 11 heterologous enzymes in a cascade supporting CO2 fixation & growth! www.biorxiv.org/content/10.1...

How does Pseudomonas putida keep its redox balance steady across different metabolic states? Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios. www.biorxiv.org/content/10.1...

250,000 people took part in the NO KINGS march in Chicago, with more than 7m marching in 2,700 locations US-wide, according to organisers @indivisible.org, as Americans increasingly resist Trump's authoritarianism.

🚀 Thrilled to share our new Nature Communications article: “A blueprint for designing the next-generation of synthetic C1 microbes” We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts. 🔗 www.nature.com/articles/s41... A short 🧵1/4

🚨 New paper in mBio! We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10.... Here’s a teaser of how we did it 🧵👇1/5

1/ 🚨 New preprint! We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1... #Metabolomics #MassSpec #SystemsBiology

Now published in Microbial Biotechnology! enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10....

The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇

Great work by @oscarpuiggene.bsky.social from our @labnikel.bsky.social showing methanol assimilation in P. Putida via enhanced serine–threonine cycle, do not miss this read!

Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍 Engaging talks, exciting science, and amazing connections — we’re full of new ideas! 🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆 🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!

A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis -in NPJ Biofilms and Microbiomes by David Ranava et al from Northwestern University / Mee-Ngan Yap, Arthur Prindle www.nature.com/articles/s41...

The TOLERATE team met at DTU (Denmark) for a productive General Assembly. New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead. More info 👉 tolerate-eu-project.com Subscribe 👉 eepurl.com/i6uufM #EUProjects

www.sciencenews.dk/en/designed-... Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains

Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc

Interested in engineering bacteria to turn #C1feedstocks into high-value chemicals for #bioproduction? Here is a thread summarizing our latest review on the roadblocks and how we might bio-hack our way past them 🧵 1/n academic.oup.com/femsre/advan...

Out now! Our latest paper where we show a "one-pot" labelling technique which allows for simultaneous mechanical and fluorescent readouts from a protein (Hsp90): onlinelibrary.wiley.com/doi/10.1002/... Congratulations to everyone who worked on this, especially Laura!

Microbes like Cupriavidus necator naturally grow on #renewable feedstocks ♻️🧫 (CO₂, formate, hydrogen), yet their potential remains untapped due to the lack of efficient genetic tools... 1/2 www.cell.com/trends/biote...

Our brand new CRISPR/Cas toolkit for genome editing of Cupriavidus necator is out🦠🧬 A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!) Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n www.cell.com/trends/biote...

The utilization of #C1-feedstocks, such as methanol, by non-model bacteria demands an in-depth analysis of design alternatives, a modular strategy to identify bottlenecks and efficient solutions within the host #metabolism of the chosen host... www.biorxiv.org/content/10.1...

With great collaborations come great outcomes! A fine example from @eorsi.bsky.social from @pabnik.bsky.social's lab et collaborators of how to design, build and develop cell biosensors for value-added chemicals (related to synthetic C1 assimilation, of course)! 🧪

Computation-aided designs enable developing auxotrophic #metabolic #sensors for wide-range glyoxylate and glycolate detection—spearheaded by Enrico Orsi w/a choral assembly of cool collaborators @naturecomms.bsky.social #SynBio #OpenAccess www.nature.com/articles/s41...

Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint.bsky.social led by Òscar Puiggené #SynBio #Methylotrophy #CircularEconomy www.biorxiv.org/content/10.1...

Super excited to see my main PhD project finally out in @naturemicrobiol.bsky.social www.nature.com/articles/s41.... We show for the first time that a synthetic and engineered pathway can indeed exceed nature at one-carbon fixation.

One-carbon fixation at the next level! A piece of work that opens up new scenarios for engineering bacteria for sustainable biomanufacturing! ⚗️🦠♻️

New: Using F-tractin to visualize #actin in living cells? Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner, and Alexander Belyy @alexanderbelyy.bsky.social determined the F-tractin-F-actin structure and showed that this probe is nearly identical to #Lifeact. https://buff.ly/3EIqAur #cytoskeleton

great title!! And the paper is cool too. www.nature.com/articles/s41...

#Microbes Saving Lives & Reducing Suffering—led by KenTimmis, this is a call to action for understanding and exploiting microbes & their role in helping humans at the face of current challenges as a global society @microbiotech.bsky.social enviromicro-journals.onlinelibrary.wiley.com/doi/full/10....