Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold. Want to get a tough sample, like lung, looking like this? Read more! www.biorxiv.org/content/10.1...

Domesticated animals have pulled our heavy carts and turned our large mills for centuries. But what about the opposite end of the spectrum—what if the wheel you want to turn is so small you can’t see it? Turns out we can harness the power of bacteria to power the world’s smallest machines. 1/7 ⚛️🧪

A protein kinase D-Ikaros pathway linking invariant TCR to PLZF during iNKT cell development @jem.org @uosaka.bsky.social rupress.org/jem/article-...

The lab is looking for two PhD students. Apply to join the team and do some exciting Immunology research.

Open Post-doc position in the lab. Apply to join the team and do some exciting Immunology research.

A number of open positions in the lab. Apply to join the team and do some exciting Immunology research.

The Junior PI call @ccii-kyoto.bsky.social is open until the end of the month. Join us in Kyoto! 👇 #Immunology #Immunotherapy #Immunosky

Migrating immune cells globally coordinate protrusive forces @natimmunol.nature.com www.nature.com/articles/s41...

🚨 New Paper in European Journal of Immunology! We show that NKG7 is a robust marker of cytotoxicity, outperforming GZMB and PRF1 across immune cell types and tissues! Thanks to all co-authors, @nsssusanna.bsky.social, @M.Hoelzel & @eomesodermin.bsky.social 📖 onlinelibrary.wiley.com/doi/10.1002/...

NKG7 is a stable marker of cytotoxicity across immune contexts and within the tumor microenvironment www.biorxiv.org/content/10.1... @eomesodermin.bsky.social @nsssusanna.bsky.social @rturiello.bsky.social

🚨 Job alert 🚨 1 PhD + 1 Technician Our lab is looking for motivated candidates interested in understanding the impact of diets and lifestyle on tumor development, anti-tumor immunity and metastasis! Apply and join our team! Info below 👇

🚨Open positions! The group of Laura Surace from the University Hospital Bonn is looking for a 🧑‍🔬 PhD student in metabolic regulation of tissue-resident immune cells during metastasis 🧑‍🔬 and a Technical Assistant Application Deadline is March 31! #job #science #PhD #immunology #openposition

icCITE-seq (intracellular cellular indexing of transcriptomes and epitopes), a scalable method that simultaneously measures surface and intracellular protein levels alongside gene expression across thousands of cells doi.org/10.1101/2025...

Our paper on nurturing a #PositiveResearchCulture within your #research #team published by @wellcometrust.bsky.social gives useful tips for your lab. As well as making you a good team leader, nurturing culture pays off in building your science: 1/11 wellcomeopenresearch.org/articles/9-3...

scRepertoire 2: Enhanced and Efficient Toolkit for Single-Cell Immune Profiling www.biorxiv.org/content/10.1...

@cellcellpress.bsky.social resource: Global organelle profiling reveals subcellular localization and remodeling at proteome scale www.cell.com/cell/fulltex... @marcoyannic.bsky.social

Timescales in Cell Biology

Here is out first attempt to understand #TCR #repertoire in human tissues -Tissue determinants of the human T cell receptor repertoire Around 6 million T cells profiled at #single #cell #level www.biorxiv.org/content/10.1...

Cancer escapes immunotherapies by mutating its surface, meaning even groundbreaking #CARTCell therapies fail too often. But what if #GenerativeAI could design CAR-T with de novo binders faster, targeting resistant tumors? 🧵 www.biorxiv.org/content/10.1... (1)

How do T cells reach melanoma brain tumors? Using in vivo microscopy 🔬🐭, we discovered a new route for T cells to enter the brain via distinct peri-tumoral venous vessels. We recently published this work in Immunity: authors.elsevier.com/sd/article/S...

How long does an mRNA stay in the nucleus? How long does it stay in the cytoplasm? In an amazing collaboration with @landthalerm.bsky.social, we used metabolic labeling, cell fractionation and mathematical modeling to quantify mRNA flow through the cell. Finally out in MSB: doi.org/10.1038/s443...

Here Malcolm Sim and Eric Long discuss peptide sensitivity of the HLA/KIR interaction. For anyone interested in this evolving subject (i.e. immune mediated disease), this review explains the difficult concepts really nicely and introduces a couple of models. #immunology www.cell.com/trends/immun...

Happy to transition to this platform to discuss science and share our fascination. The movie below shows a dendritic cell, highlighting the remarkable ability of immune cells to navigate their cell body and to organise their intracellular cargo (nucleus in cyan). (Kroll et al, EMBO J 2023)

1/ 🧬 Single-cell genomics reveals biological variations beyond cell types. Unveiling these in separate latent dim’s is known as disentanglement. Led by @AmirAliMoinfar, we develop DRVI to learn nonlinear, disentangled & interpretable latent spaces. biorxiv.org/content/10.1101/2024.11.06.622266v1

How do organelles fit together inside a crowded cell? Answering this question requires a way to image many organelles in a whole cell at high resolution with high throughput. Our new preprint shows how to do this in yeast using soft x-ray tomography www.biorxiv.org/content/10.1...

This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.

To populate my Bluesky and people know what I do: I am an MD turned immunology "basic" researcher. In the past I did adoptive T cell therapy and then fate mapping in the Buchholz lab in Munich: CD8+ T cells in MCMV: www.nature.com/articles/s41...

To introduce myself and what I do: in my PhD @romagnanilab.bsky.social, I found that human NK cells maintain clonal immune memory to CMV infection www.nature.com/articles/s41...

NK cells underpin the efficacy of combined PD-1/CTLA-4 blockade in brain metastases by orchestrating a molecular "responder" profile in tumors & controlling intratumoral abundance of CD8+ T cells by regulating T cell trafficking mediators doi.org/10.1136/jitc...

Tolerance by eviction "we report that, in mice, CD8 T cell tolerance instead results from premature thymic eviction of immature autoreactive CD8 thymocytes into the periphery, where they differentiate into self-tolerant mature CD8 T cells" #Immunology #Immunosky

New preprint 📣📣 Together with Monika Wolker's lab @sanquin Amsterdam, we optimized a smFISH protocol for T cells. We measured cytokines IFNG/TNF expression and localization during T cell activation and optimized an imaging pipeline for quantification. www.biorxiv.org/content/10.1...

Cancer Immunology: bsky.app/starter-pack... A repertoire of immunologists II: bsky.app/starter-pack... Immunometabolism: bsky.app/starter-pack... Immunoengineering: bsky.app/starter-pack... A repertoire of immunologists: bsky.app/starter-pack...

I am excited to share our latest paper. We profiled CD8+ TILs from HPV- HNSCC patients using scRNA/TCRseq. In addition, we performed an ex vivo stimulation of cells to capture their transcriptional response. 1/n onlinelibrary.wiley.com/doi/10.1002/...

Putting LIPSTIC on CARTs "We reasoned that target-induced labelling of CAR molecules within the immunologic synapse, instead of surface CD8 expression levels, could be used to distinguish and characterize first-division proximal- and distal-daughter CARTs" #Immunology www.nature.com/articles/s41...