I have little time for LLMs for the same reason I have little time for liars. Having to double-check every item is exhausting. I’d rather not bother.

"Range extender mediates long-distance enhancer activity" by @evgenykvon.bsky.social 's team, led by @gracebower.bsky.social is one of my papers of the year. Discovery of a new class of cis-regulatory elements that modulate the regulatory activity and reach of enhancers. doi.org/10.1038/s415...

My lab is hiring a postdoc (ERC funded)! If you're interested, get in touch!

I've been successfully using Burton's buffers with dawn and no Triton for staining an endosomally localized membrane protein.

I could not recommend this more strongly! A really unique opportunity to join a top immunology lab in Cambridge, doing innovative science. A great PI to work with!

I could not recommend this more strongly! A really unique opportunity to join a top immunology lab in Cambridge, doing innovative science. A great PI to work with!

Traffic jams are brutal this time of the year in Cambridge

I feel like I've been doing a lot of presentations this year. Just added it up - 38 scientific seminars in 11 countries, plus 21 career development or research culture workshops, plus 12 undergraduate lectures, plus 2 public engagement presentations. More than one a week!

Our latest article celebrates some of our alumni and Fellows writing to inform and entertain children and young adults, including books written by joannechocolat.bsky.social, labliston.bsky.social and many others! Read more: www.caths.cam.ac.uk/writing-for-...

In our latest editorial, @labliston.bsky.social celebrates the perseverance behind the 2025 #NobelPrize in Physiology or Medicine. Learn how regulatory T cells act as the immune system's 'brakes', and how immunology and genetics came together to uncover these elusive cells.

Catz Fellow Prof. Adrian Liston (2023) & colleagues have uncovered major differences in one type of white blood cell in people living with #Alzheimers disease – new evidence about how this disease progresses & could be treated: www.caths.cam.ac.uk/...#Alzheimers@labliston.bsky.social @campathology.bsky.social

Day 4 and the final day of the BSI saw a fantastic talk from @labliston.bsky.social about Tregs in the brain and an excellent session devoted to “Barriers, bugs, and breakdowns” - right up our street! Thank you to all organisers and presenters @britsocimm.bsky.social for a great week in Liverpool🫶

I am torn on this. Yes, bullying is a major problem in UK academia, with little appetite of leadership to address it. *But* to boil research culture down to a REF returnable metric, we need good metrics of research culture. Just creating tick-boxes that reward the most cynical players won't help.

Beautiful talk by Matt Hepworth, so many exciting things happening at barrier surfaces. @hepworth-lab.bsky.social #BSI2025 @britsocimm.bsky.social @lydiabeckeriii.bsky.social

Fantastic talk by @labliston.bsky.social at #BSI25! Incredible prospects for IL-2 gene therapy and fascinating tools for tracing CRISPR knockouts. Brilliant! @britsocimm.bsky.social

#BSI25 Neuro-immune crosstalk stellar talks by @labliston.bsky.social, Henrique Veiga Fernandes @champalimaudr.bsky.social & Menna Clatworthy @medcambridge.bsky.social/ @mrclmb.bsky.social

Very cool talk on Tips and Tricks on Spectral Flow Cytometry by Dr. Oliver Burton (@labliston.bsky.social) in the ThemeNight of the Flow Cytometry Core Facility of the @unibonn.bsky.social!

My first Cambridge graduation, becoming Doctoris in Scientiis

Our new study on #systemsimmunology analysis of #Alzheimers patients is out now at @alzdemjournals.bsky.social! This is the culmination of a collaboration with Rik Vandenberghe, Stephanie Humblet-Baron and Lidia Yshii's labs at KU Leuven. 🧵1/8 alz-journals.onlinelibrary.wiley.com/doi/10.1002/...

Clues about aetiology of Alzheimer’s from an immune system perspective

Our new study on #systemsimmunology analysis of #Alzheimers patients is out now at @alzdemjournals.bsky.social! This is the culmination of a collaboration with Rik Vandenberghe, Stephanie Humblet-Baron and Lidia Yshii's labs at KU Leuven. 🧵1/8 alz-journals.onlinelibrary.wiley.com/doi/10.1002/...

Thank you editors for improving the readability of our paper by removing acronyms. Now we grow our cluster of differentiation 4+ Forkhead box 3+ cells in Roswell Park Memorial Institute media with phorbol 12-myristate 13-acetate. TIL, RPMI media is actually Roswell Park Memorial Institute media

An education in tolerance: the 2025 Nobel Prize in Physiology or Medicine journals.biologists.com/dmm/article/...

How can we optimize the delivery of IL-2 to traumatic brain injury patients? www.tandfonline.com/doi/10.1080/...

Adrian Liston, James Dooley und Oliver T. Burton von der Cambridge Universität nutzen Fairy-Spülmittel zur Entwicklung eines optimierten Puffers für die Durchflusszytometrie. Mehr im Artikel von Andrea Pitzschke 👉 www.laborjournal.de/editorials/3... @campathology.bsky.social @labliston.bsky.social

@labliston.bsky.social shared a paper they had written using dish soap as a permeabiliser for Flow Cytometry: currentprotocols.onlinelibrary.wiley.com/doi/10.1002/... Someone I work with tried it, and it had the additional benefit of reducing Autofluorescence, which will give you better data!

How can we optimize the delivery of IL-2 to traumatic brain injury patients? www.tandfonline.com/doi/10.1080/...

November 8th marks Intersex Day of Remembrance. Intersex Day of Remembrance is a day to remember those who are no longer with us and highlight issues that the Intersex community face. We stand in solidarity with our Intersex siblings, team members and supporters! #IntersexDayOfRemembrance

I love a good recipe 💚✨️

It is not often that a new flow cytometry protocol makes the newspaper! I also got a kick out of seeing our science tagged as "quirky"! www.cambridgeindependent.co.uk/news/fairy-l...

Foreign Scholars Skip US Conferences “I don’t agree with the politicization of scientific funding, the destruction of data collection and openness... and I feel like traveling to America at this point is a partial endorsement of what’s going on,” Liston said. www.insidehighered.com/news/faculty...

Foreign Scholars Skip US Conferences “I don’t agree with the politicization of scientific funding, the destruction of data collection and openness... and I feel like traveling to America at this point is a partial endorsement of what’s going on,” Liston said. www.insidehighered.com/news/faculty...

Fascinating talk tonight from Malala Yousafzai

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As the editor of CSH Protocols, I wholeheartedly agree. And if you have an idea for a topic to do a protocols collection on, or know of a field that would benefit from such a collection, let's talk!

Haven't read this one yet, but looks like a fantastic resource for everyone working with complex #spectral #flowcytometry panels!

A small 🧵 encouraging people to publish protocols. Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work. www.biorxiv.org/content/10.1...

A small 🧵 encouraging people to publish protocols. Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work. www.biorxiv.org/content/10.1...

Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold. Want to get a tough sample, like lung, looking like this? Read more! www.biorxiv.org/content/10.1...

Today I accidentally discovered the ySG podcast "Climbing the Immunological Ladder". Highlight: an absolutely incredible episode with Adrian Liston @labliston.bsky.social: "Caring about people and caring about their growth ends up being the most impactful thing you can do in science"

The problem is that spectral unmixing is complex. The basis is a linear regression of positive versus negative signals, a highly error-prone process. This issue is largely solved by the use of robust linear regression with iterative rounds of improvement (AutoSpill): www.nature.com/articles/s41...

Three addition problems: 1)This unmixing solution still requires ideal positive-negative matching to find the right linear regression. 2) Cells have variation in background fluorescence 3) Fluorophores actually stuck on cells have variation in emissions #AutoSpectral solves these!

Huge credit to @colibricytometry.bsky.social, who has spent years working on every aspect of the spectral flow cytometry pipeline. Follow the Colibri blog to get tips in real-time, pre-pre-print! www.colibri-cytometry.com

First, pos-neg gating. Ideal controls are your actual antibodies on the actual cells. However different antibodies bind different cells (see: CD4 vs XCR1 below), meaning they have different backgrounds to control. AutoSpectral matches the FSC-SSC of each pos to a suitable neg: with results!

The whole pipeline is available right now on GitHub. It includes comprehensive notes on every step from installation to utilisation. github.com/DrCytometer/... Hopefully soon (like with AutoSpill) it becomes standard on commercial platforms too. But don't wait - fix your data issues today!