🌈Proud to share our new paper in ACS Photonics! We developed a Spectral Confocal Spinning Disk ISM system for simultaneous high-resolution multi-color imaging using a custom linear Amici prism & GPU Python pipeline. Congrats to Lanna Bram & Jonathan Jeffet! 👏 🔗 doi.org/10.1021/acsp...

New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

Get to know Thomas Schlichthärle, our newly appointed professor of #AI-guided #ProteinDesign, in the latest NewIn episode. He wants to use custom-designed #proteins to advance the development of new #medicines: go.tum.de/484027 #HightechAgendaBayern ▶️ youtu.be/G-pt28HJpA8 📹& 📷ProLehre

Super cool work !

Congrats to Juliana! She measures in ACS Nano the rate constants of nanosensors on the single molecule level. She found two kinetic pathways and one can use this knowledge for Kinetically Improved Sensor Selectivity (KISS). @solvationsci.bsky.social @ruhr-uni-bochum.de tinyurl.com/738ypxf9

Excited that our work on Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells is now published in ACS Photonics! doi.org/10.1021/acsp... @pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Link ot he @biorxivpreprint.bsky.social is here: www.biorxiv.org/content/10.6...

A cool thing was the FRET-based diffusion analysis that allowed a separate analysis of MET receptor monomers and dimers👇🏻

Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy. pubs.acs.org/doi/10.1021/...

This #Halloween 🦙🧛‍♂️ Alpacula brewed up #SMLM treat! No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB

We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput. 👉 BioRxiv: www.biorxiv.org/content/10.1... #SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing

NEW PREPRINT: Spatial mapping of cell-surface protein glycosylation at molecular resolution 🍭🍬🔬 For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime www.biorxiv.org/content/10.1...

Fiery kickoff for the 30th Single Molecule Workshop! I’m super excited to see so many great scientists and big names in the field gathered here in Berlin. Let’s go #WS30 🚀 #WS30 #SingleMolecule #Spectroscopy #Microscopy

Soo on stage presenting self-labeling enzymes with weak-affinity fluorophore labels for (1) high-density live SMLM using DeepSTORM (doi.org/10.1016/j.bp...) and (2) molecular counting ... great collaboration with @kjohnsson.bsky.social @juliankompa.bsky.social 🙏 #PQ30 @picoquant.bsky.social

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1... Read the thread below for a summary of our findings 🧵1/11

Previous preprint is now published in RSC Chemical Biology. If you are interested in plasma membrane labeling, see the paper! 👇 pubs.rsc.org/en/content/a...

Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!

🚨 New Preprint! Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons. ▶️ www.biorxiv.org/content/10.1...

🚨 New preprint on bioRxiv! 🚨 Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation. @lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future. 🧵👇 doi.org/10.1101/2025...

Proud to see this preprint out! First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.

Excited to share — our project from @labtinnefeld.bsky.social with Heinrich Grabmayr from @jungmannlab.bsky.social has been selected for the CeNS Investment Fund from @cens-lmu.bsky.social ! www.cens.lmu.de/en/research/...

💥 We have 3 positions open in my lab in Basel, Switzerland🇨🇭: #nanopores #smFRET #biomolecular #dynamics #singleMolecules We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech. Please share broadly, thank you!🤝 schmid.chemie.unibas.ch

Great lab and great PI to start with ...!

🔬🧬 New in @natcomms.nature.com‬: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess  www.nature.com/articles/s41... @ellenberglab.bsky.social @jungmannlab.bsky.social #Genomics #SuperResolution

A "Small" update on studying the coating of single DNA nanostructures with a fluorescence lifetime sensor! 🧬🔬 Was a fun project at the interface of DNA nanotech and single molecule photophysics working in a great team! 🤓 🙌 @ameliehj.bsky.social onlinelibrary.wiley.com/doi/10.1002/...

It's here! ✨ The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF ➡️ Open link: rdcu.be/euvEw

🎉 Major achievement for #LMU in the Excellence Strategy: All seven proposed Clusters of Excellence have successfully passed the review process and will be funded for seven years as of 2026. An outstanding result! 🎉 #ExcellenceCluster #LMUMunich #research Read more here: www.lmu.de/en/newsroom/...

The #ClustersOfExcellence have been selected: today, the Excellence Commission approved 70 projects for funding. 45 clusters will continue and 25 will be newly established. Funding starts on 1 Jan 2026 for 7 years, with €539 million per year. The full list: www.dfg.de/resource/blo... 1/3

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...

🚨 Join us in shaping the future of #SmartMicroscopy! We're hiring PhD & fellowship positions at the intersection of #photonics, electronics & #AI. Check out our works in this field doi.org/10.1038/s414... www.nature.com/articles/s41... doi.org/10.1101/2024... Please share! #SPADarray #Microscopy

#newPI and time is running. It has already been a month since I officially joined the University of Warwick as Assistant Professor. I am excited to announce and build the Fluorescence and Membrane Dynamics (FMD) Lab: warwick.ac.uk/fac/sci/med/...

📢 The University of Frankfurt (Germany) is hiring a Full Professor in Organic Synthesis -->Cutting-edge research in an open, collaborative environment? Check out the call and join one of Germany’s leading research universities. #AcademicJobs #FacultyPosition www.nature.com/naturecareer...

Collaborative work with the Shechtman Lab and @heilemannlab.bsky.social. Check it out here: www.biorxiv.org/content/10.1...

🚨We have a 4-year ERC-funded PhD position available in the beautiful city of Leuven, Belgium, for building artificial nanomachines in lipid membranes. Check out the details and apply here: www.kuleuven.be/personeel/jo... Feel free to email me for more information!

𝙄𝙣𝙩𝙧𝙤𝙙𝙪𝙘𝙞𝙣𝙜 𝘼𝙪𝙩𝙤𝙢𝙖𝙩𝙚𝙙 𝙎𝙥𝙚𝙘𝙩𝙧𝙖𝙡 𝘿𝙚𝙢𝙞𝙭𝙞𝙣𝙜 Our new SAFe platforms now feature automated spectral demixing—a breakthrough that: ✅ Enhances accuracy ✅ Eliminates bias ✅ Delivers clearer, more reliable data effortlessly 🚀 Coming soon! #SMLM #SuperResolution #Bioimaging #imaging #Microscopy

We have a new review out! In this Microscopy & Microanalysis piece, Louisa Mezache and I survey some of the latest commercially available microscope technologies: Nikon NSPARC, Re-scan Gaia, CSR Biotech MI-SIM, refinements to STED, SMLM from Bruker and Abbelight… Check it out with free access:

A comprehensive review on covalent chemical strategies for protein self-labeling from @kjohnsson.bsky.social's lab: HaloTag, SNAP-tag et al. www.annualreviews.org/content/jour...

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM

We've just published a new scientific preprint that dives into the spatial glycome using super-resolution microscopy. Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1... #dSTORM #SMLM #glycobiology

👩‍🎓 👨‍🎓 #Recrutement #These #PhD La campagne de recrutement de l'ED Physique et Sciences de la matière est ouverte @univ-amu.fr Infos sur nos postes & les pièces à fournir 🇫🇷 🇬🇧 : www.fresnel.fr/wp/offres/of... Contactez au + vite le(s) chercheur(s) référent(s) du sujet qui vous intéresse ! 📸 Eléa Ropiot

Come work with us on new approaches for single-molecule microscopy! -PhD offer in collaboration with Christian Specht: Synuclein diffusion analysis in neurons using event-based super-resolution microscopy - Short software engineer contract to work on EveSMLM 🧪 #AcademicSky #SMLM #Super-resolution

New paper out💥 in Nano Letters @pubs.acs.org High throughput single particle profiler with simple confocal imaging. We used this new method to study protein-lipid interactions, drug membrane interactions and biophysics of small particles from human donors: pubs.acs.org/doi/10.1021/...

New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today: SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion! www.sciencedirect.com/science/arti... 1/

Tracking ER proteins _continously_ in living cells using reversible protein labels ... the #MicroscopyMonday edition ...

Incredible new work from Yongdeng Zhang demonstrating live 4Pi SIM--and in two colors! www.nature.com/articles/s41...

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇 www.biorxiv.org/content/10.1...

It's celebration time: our first contribution to a better understanding of RNA structure and dynamics in biomolecular condensates together with Alberti lab - cool data from in vitro to in condenso to in vivo take a look 👇 @cmcb-tud.bsky.social @tudresden.bsky.social www.cell.com/molecular-ce...