Claudio Alfieri
@claudioalfieri.bsky.social
📤 177
📥 415
📝 4
Amazing work from my neighbour's lab! Many congrats to all the people involved! 👏👏👏👏
add a skeleton here at some point
26 days ago
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Claudio Alfieri
TJ McCorvie
about 2 months ago
Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures.
#CryoEM
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Roger Castells
2 months ago
This is super cool and great investigative work! Thanks Oli and co!
#smallparticles
#cryoEM
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Claudio Alfieri
Basil Greber
3 months ago
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey
@icr.ac.uk
and with some small-complex-plus-peptide
#cryoEM
by
@natalia-mg.bsky.social
from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
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High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications
Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...
https://www.nature.com/articles/s41467-025-62765-z
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Claudio Alfieri
Svetlana Dodonova
6 months ago
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by
@harshranawat.bsky.social
!
www.biorxiv.org/content/10.1...
#Chromatin
#Asgard
#Archaea
#cryoEM
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Claudio Alfieri
Bussi Lab
6 months ago
Our integrative approach using
#MD
and
#cryoEM
data to construct structural ensembles of
#RNA
just published on
@natcomms.bsky.social
doi.org/10.1038/s414...
Lead by Elisa Posani, with
@magistratolab.bsky.social
@bonomimax.bsky.social
@pjanos.bsky.social
@navtejtoor.bsky.social
and Daniel Haack 🎉
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Claudio Alfieri
Vikram Alva
6 months ago
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone"
www.biorxiv.org/content/10.1...
@mpi-bio-fml.bsky.social
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Claudio Alfieri
Sebastian Glatt
7 months ago
We are very excited about our latest work, which just came out
@embojournal.org
–
www.embopress.org/doi/full/10....
. The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with
@jmbujnicki.bsky.social
…a short🧵
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Determining the effects of pseudouridine incorporation on human tRNAs | The EMBO Journal
imageimagetRNAs harbour a complex set of modifications which are important for their function. Here, single particle cryo-EM, biophysics, and computational analyses of the effects of pseudouridine inc...
https://www.embopress.org/doi/full/10.1038/s44318-025-00443-y
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Claudio Alfieri
Jan Philipp Kreysing
7 months ago
New preprint on 3D heterochromatin architecture in human cells! Great collab with
@sergiocruzleon.bsky.social
&
@johannesbetz.bsky.social
from
@hummerlab.bsky.social
,
@marinalusic.bsky.social
& the Turoňová lab. Many thanks to my supervisor
@becklab.bsky.social
. bioRxiv:
tinyurl.com/3a74uanv
🧵👇
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Claudio Alfieri
Structura Biotechnology
7 months ago
#CryoSPARC
v4.7 is out! 🚀 Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more! Full changelog:
cryosparc.com/updates
#cryoEM
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Claudio Alfieri
Lucas Farnung
8 months ago
🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes.
@jonmarkert.bsky.social
doi.org/10.1016/j.jb...
#Chromatin
#SIRT6
#CryoEM
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Claudio Alfieri
Suyang Zhang
8 months ago
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM
#transcription
#splicing
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Claudio Alfieri
John Helmann
8 months ago
How does MntR function as both repressor and activator with different Mn(II) sensitivity and cooperativity? Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky
#ChemSky
#cryoEM
www.nature.com/articles/s41...
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Structural basis for transcription activation through cooperative recruitment of MntR - Nature Communications
MntR is a protein that can repress the expression of Mn2+ uptake transporters and activate the expression of efflux proteins. Here, the authors use cryo-EM and other biochemical approaches to establis...
https://www.nature.com/articles/s41467-025-57412-6
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Claudio Alfieri
Pedro Beltrao
9 months ago
Sad to know about the passing of Béla Novák. This review from Tyson, Chen and Novak was such a revelation for me as an introduction to control theory in biological systems. A classic that will remain relevant for many years to come.
www.sciencedirect.com/science/arti...
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Claudio Alfieri
EMBL-EBI
9 months ago
📢 We’re looking for a new Team Leader!
@enasequence.bsky.social
is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources. Interested?👇
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Claudio Alfieri
Brian Liau
9 months ago
Today in
@nature.com
we share our back-to-back stories with Ning Zheng’s lab revealing chemical-genetic convergence between a molecular glue degrader & E3 ligase cancer mutations. 1/5
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Claudio Alfieri
@tim_stasevich
9 months ago
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
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Tanmay Bharat
9 months ago
The mechanics of a continuous self-assembling surface-layer aids cell division in an archaeon Tight collaboration with
@buzzbaum.bsky.social
, Sulfolobus cryo-ET from
@idocaspy.bsky.social
www.biorxiv.org/content/10.1...
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Claudio Alfieri
Carter Lab
9 months ago
Today in
#journalclub
, we looked at the new study from Nguyen et al. showing how Cryo-EM captures early steps in dynein activation by LIS1. They identified a novel ‘Pre-Chi’ intermediate, shedding light on how LIS1 relieves dynein autoinhibition!
www.biorxiv.org/content/10.1...
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Cryo-EM captures early intermediate steps in dynein activation by LIS1
Cytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited “Phi” state and requires conformational changes to assemble with its cofa...
https://www.biorxiv.org/content/10.1101/2025.01.10.632485v1
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At
#EMBOinsitu
and looking for a motivated postdoc joining an interdisciplinary project in my lab:
jobs.icr.ac.uk/vacancies/11...
the-alfieri-lab.co.uk
#cryoem
#cryotem
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Postdoctoral Training Fellow in Chelsea | The Institute of Cancer Research
View details and apply for this Postdoctoral Training Fellow vacancy in Chelsea . Salary: Dependent on relevant Postdoctoral experience. Future progression is based on annual performan...
https://jobs.icr.ac.uk/vacancies/1107/postdoctoral-training-fellow.html
9 months ago
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Claudio Alfieri
11 months ago
Our
#CryoET
paper on ciliary rootlet ultrastructure from mouse retina is now out in
@elife.bsky.social
! Have a peek with this video or dive deeper at
bit.ly/3ZJzhgc
. Big thanks to
@carter-lab.bsky.social
and
#teamtomo
.
#CryoET
#CryoEM
#Cilia
#Rootlet
#Centriole
#Research
#Science
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Claudio Alfieri
Basil Greber
11 months ago
New pre-print! To support a CDK-cyclin docking motif discovery and characterisation project led by Norman Davey and colleagues,
@natalia-mg.bsky.social
in my lab determined the
#cryoEM
structures of three peptide-bound CDK2-cyclin A complexes. Check it out!
www.biorxiv.org/content/10.1...
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High-throughput discovery and deep characterization of cyclin-CDK docking motifs
Cyclin-CDKs are master regulators of cell division. In addition to directly activating the CDK, the cyclin subunit regulates CDK specificity by binding short peptide “docking” motifs in CDK substrates...
https://www.biorxiv.org/content/10.1101/2024.12.03.625240v1.full
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Claudio Alfieri
Basil Greber
11 months ago
Thrilled to share that I have been selected as one of this year's new EMBO Young Investigators. I look forward to meeting many of the other Young Investigators in the coming years, and I am most grateful to the members of my laboratory at the ICR who made this possible.
www.embo.org/press-releas...
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Twenty-seven scientists become EMBO Young Investigators – Press releases – EMBO
The group leaders join an international network of nearly 800 life scientists and receive financial support
https://www.embo.org/press-releases/twenty-seven-scientists-become-embo-young-investigators/
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