For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy

Mario Ledesma-Terrón, Diego Pérez-Dones, David Míguez and colleagues @cbm-csic-uam.bsky.social @ifimacuam.bsky.social present OSCAR, a framework to quantify 3D stacks with high cellular density. journals.biologists.com/jcs/article-...

Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...

My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing 🔵 lifeact & 🔴 RFP-nls Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨ 🔗 www.nikonsmallworld.com/galleries/20... #NikonSmallWorld #Microscopy #ScienceArt

See this? This = implanting mouse embryo. Usually this happens inside its mother and is invisible to us, but we can actually watch implantation ex vivo with the hope of understanding why implantation goes awry in embryos of older women. A 🧵...

ER exit sites are dynamic ER subdomains that integrate signals, reshape membranes and steer secretion. Twelve groups in the field came together to map their diversity, regulation, unanswered questions and future directions. www.nature.com/articles/s41...

You're watching 12 hrs of live cell imaging compressed into a GIF. A multi-nucleated mouse pre-adipocyte tries to undergo mitosis, triggers a checkpoint because it has too much DNA, & undergoes apoptosis. The last stage is called "blebbing", which is wonderfully descriptive. (🔬: NanoLive Imaging)

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on! #TeamTomo #PlantScience 🧪 🧶🧬 🌾 elifesciences.org/articles/105... 1/🧵

Comparing single-cell #SpatialTranscriptomics methods on FFPE human tumor #TissueMicroArray CosMx 1k vs MERFISH 500 vs Xenium-UM/-MM 339 93 common genes lung adenocarcinoma pleural mesothelioma Corr. Bulk RNAseq, GeoMx #NatureComms 2025 www.nature.com/articles/s41...

A blood vessel in the brain for #FluorescenceFriday! Why am I looking at these in the microscope? (1/3)

For this #FluorescenceFriday, a gorgeous image of an adult mouse kidney labeled with AQP2 and alpha SMA antibodies. AQP2 (green) marks the collecting duct and distal connecting segment while SMA marks the arterial tree. Courtesy of talented postdoc Sarah McLarnon.

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

1/12 A very special moment for me! 🎉 My first paper as corresponding author—a @jcb.org Perspective on how contact-based decision-making in collective cell migration can itself encode blueprints for complex patterns and shapes. rupress.org/jcb/article/... #cellbio #devbio #science 🧵for details

An iPSC cardiac myocyte (heart muscle cell) photographed through a microscope. That yellow thing is the DNA in the nucleus. The cyan things are the motor domains of myosin II, the molecular motor that drives muscle contraction. #CellBiology

Ok one more for #MicroscopyMonday b/c it was a very fun afternoon. This is from live tissue. Auditory neurons in magenta & their friends (including satellite cells) in green. Big thx to @jessdixonneuro.bsky.social for being generous w/ reagents #ScienceSisters Imaged on FLIMmer @ HMS MicRoN core.

AI investment is booming, but what about understanding human intelligence? 15% of the NIH grants cancelled this year are in #Neuroscience. Over $323 million lost. The image below is from a 94 Y.O donor, part of a project to understand the 'superager brain' that lost its funding. 💔 #MicroscopyMonday

Field notes from a weekend PI at the #microscope: vesicle cargo lanes lighting up in real time like @FedEx. This is the ML1N PX domain in a fibroblast. Hot damn, there’s mind-blowing action under the surface of cells: this video shows 2 min of time 🤯 #Cellbio #MicroscopyMonday #SciArt #SciComm

A ring of cells deforms into a triangular keyhole in just 15 minutes. Meet the hindgut, a model for boundary-driven morphogenesis! Out now in @pnas.org at doi.org/10.1073/pnas... with @zhaoshh.bsky.social, Alex Jacinto, Eric Wieschaus, Stas Shvartsman, @lepuslapis.bsky.social (1/8)

1🧵 Excited to share my first PhD paper, published in @dev-journal.bsky.social What if fish gills —often overlook —hold secrets about development, patterning, and function? We uncovered how early patterning shapes adult gill architecture. journals.biologists.com/dev/article/... #DevBio #Zebrafish

Time to add some ExM to #FluorescenceFriday ! Sample description in alt-text.

It's #FluorescenceFriday! Another movie from our preprint, showing exocytosis of VAMP2-pHluorin (live-cell TIRF, blue) correlated with 2-color STORM of spectrins (green) and clathrin-coated pits (magenta). www.biorxiv.org/content/10.1...

Years ago we discovered that missense mutations in homomeric proteins often induces their supramolecular assembly, as in the 📽️👇 - Are such aberrant assemblies of *folded* proteins toxic? Surprisingly, NO. Cells are amazingly robust systems! Paper now online @molsystbiol.org doi.org/10.1038/s443...

Do you know what happens when you touch a carnivorous sundew plant? If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.

Despite their high rigidity, microtubules (MTs) dynamically bend and buckle in cells. MT-self repair is inherent to their metastable nature, but it remains unclear whether just inherent repair mechanisms are sufficient to maintain MT integrity🧵 (1/9) www.biorxiv.org/content/10.1...

#FluorescenceFriday Strand and branch pericytes surround the vessels of the retina👁️🔬 color=depth @flaumeye.bsky.social @urneuroscience.bsky.social @cvsuor.bsky.social @uofrbme.bsky.social @urochestersmd.bsky.social

Happy to share that our paper on Tau- Microtubule interaction is now published in Nature Physics!🥳 We show that Tau isn’t just a microtubule stabiliser - it promotes removal of defects in the microtubule lattice. Congratulations @subhambiswas.bsky.social! www.nature.com/articles/s41...

We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)

📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure). 🔗 View the preprint article: bit.ly/47xC8NJ #Microscope #Imaging #Fluorescence

New review: Henne (@hennelab.bsky.social), Reynolds, and Prinz discuss recent discoveries and enduring mysteries in the lipid droplet field. rupress.org/jcb/article/... #Biochemistry #CellMetabolism #LipidDroplets

A pleasure to write this short review on lipid droplets for @jcb.org together with Will Prinz and amazing artist Emma Reynolds! We discuss 5 pervasive questions surrounding these remarkable little lipid organelles. doi.org/10.1083/jcb....

Thanks to all authors, including Wei Zheng and Guoqiang Yu, Amy Hu, and the Janelia @hhmijanelia.bsky.social and 4DCP communities, Maneesh Sahani, and others.

Preprint - Excited to present WHOLISTIC, which extends the concept of whole-brain functional imaging to the entire body. Pioneering work by incredibly talented Virginia Ruetten @vmsruetten.bsky.social, this platform reveals whole-organism cellular dynamics in vivo. www.biorxiv.org/content/10.1...

For #FluorescenceFriday, microtubule networks are shown in two adipocytes 🔬 #CellBiology #Microscopy

Here's a look at a field of striatal astrocytes expressing GCaMP6f-cyto from the Huda Lab for #FluorescenceFriday !

✨Nervous about development? Don’t be! 🧠 E13.5 🐁 embryo showing nerves (🩵) and endothelial cells (💙) weaving together in the developing pup 🔬 image by Nathan Burns 🧪 #FluorescenceFriday #DevBio #MouseEmbryo

Happy #FluorescenceFriday! This is membrane-GFP in Shh-expressing cells of the developing 🐭 lung. Signal is depth shaded in rainbow. 🔬 by postdoc @christinaadaly.bsky.social 👩‍🔬 🧪

For #FluorescenceFriday, microtubule networks are shown in two adipocytes 🔬 #CellBiology #Microscopy

Using expansion microscopy, we re-examined MT organization and mapped PTMs in the human airway multiciliated cells during differentiation. Basal bodies seem to serve the role of main MTOCs (2/N)

Happy #FluorescenceFriday! New gorgeous data obtained by Camilo Echeverria. We are so excited to be using new tools in the lab to better understand cytoskeletal control of #NeuralCrest cell migration. #DevBio

#FluorescenceFriday - immunostaining for cell markers in tissue section of the hippocampus in human 🧠. Sometimes, at the exact right level and angle (i.e. this one), the sea horse thing feels like not just a resemblance, but almost eerie. Image credit to RA Maddie Abramson🔬👩‍🔬🧪

Today's #FluorescenceFriday is featuring the peripheral muscle of Hofstenia miamia 💪

Happy #FluorescenceFriday! iDISCO-based tissue clearing and whole-organ Light Sheet Fluorescence Microscopy (#LSFM) reveal 3D vasculature and bile duct networks in an intact mouse liver stained for Smooth Muscle Actin (cyan; vasculature) and Cytokeratin 7 (green; bile ducts).

Bird’s eye view, literally. 🐦👁️ Glowing quail embryo eye for #FluorescenceFriday, #DevBio 🧪🔬

Microglia Megazord activated! Check these brain immune cells🔵 responding to #SARSCoV2 infection. Compare to the image on the right from a control. Neurons: 🟠NeuN, Microglia: 🔵Iba Do you know what caused this? COVID-19 Do you know what did not cause this? Vaccines. #FluorescenceFriday #NeuroCovid

Please vote for my #ARTxSCIENCE2025 submission! Voting takes 10 secs renew.science/artxscience/... My image shows stem-cell derived heart cells treated with a drug that makes cardiomyocytes (pink) proliferate—without activating fibroblasts #HeartRegeneration #Microscopy #StemCells

I found an original way to play with 3D data on ImageJ : progressive color coding! I'll share the script soon #FluorescenceFriday #Sciart #MicroscopyMonday

Chicken balancing for #3DThursday!🐔 These utricle hair cells sense gravity & straight-line motion. We segmented them with #CellposeSAM in #syGlass. 🥼: Stefan Heller Group 🏛️: Stanford @stanfordmedicine.bsky.social 🎬: syGlass.io v2.4.0, out now! #Neuroanatomy #Microscopy #3DVisualization #SciComm

Automated optogenetic control of hundreds of cells in parallel. Each cell is individually steered, collectively acting as a "tissue printer". Preprint & code out! www.biorxiv.org/content/10.1...

LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee www.sciencedirect.com/science/arti...

Supracellular control and coordination of tissue morphogenesis, through supracellular actomyosin assemblies, that is what my lab @cellbiol-mrclmb.bsky.social @pdncambridge.bsky.social and I like to dig into at the moment! Fascinating and beautiful! journals.biologists.com/dev/article/...