Live imaging of immune cell response (🟢) to Salmonella infection (🔵) in the hindbrain of a transgenic #zebrafish larva. Credit to Drs. Jade Leiba & Mai Nguyen-Chi. #ZebrafishZunday 🧪

For this #FluorescenceFriday, some endosomes imaged with MI-SIM. Green: Rab21, Magenta: F-actin, Cyan: EPLINalpha.

A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.

Happy #FluorescenceFriday! I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.

RhoG, Rac1 and Cdc42 cooperation in cell protrusion revealed by multiplexed optogenetics and biosensor imaging by Klaus M. Hahn and team: www.biorxiv.org/content/10.6...

Happy #FluorescenceFriday. I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!

happy to share our new protocol paper on wildDISCO for whole-body immunolabeling, tissue clearing & 3D imaging of intact mice at cellular resolution using standard IgG antibodies, for mapping of neuronal, vascular, lymphatic and immune systems across the entire body. www.nature.com/articles/s41...

In their Cell Science at a Glance article and poster, Kim Chi Thi Vu, Laura Bierau and Eva Herker evaherker.bsky.social unimarburg.bsky.social discuss the pro- and anti-viral functions of lipid droplets in infection. journals.biologists.com/jcs/article/...

#FluorescenceFriday From the archives: The Actin Cortex. Plasma membrane (Lck-mScarlet-I 🔴) Cortical actin (GFP-Utrophin 🔵) 1205Lus

Hey y’all 👋 repost magic appreciated. The Solecki lab is recruiting multiple postdocs at St. Jude for a chromatin imaging project at the edge of live-cell imaging, neuronal cell biology, chromatin regulation, and quantitative image analysis. Ever dreamed of touring chromatin like this? We CAN 🔥

Happy #FluorescenceFriday, here is a macrophage engulfing a deformable particle imaged on our LLSM. This project from Mira Krendel's lab and the @aicjanelia.bsky.social just came out as a preprint today! www.biorxiv.org/content/10.6...

happy #fluorescencefriday Compare with last week’s post on APOE3 mice now we have the AD APOE4 model with blood vessels in pink, amyloid in blue and microglia in green. Courtesy of the @mind-western.bsky.social @touchscreencog.bsky.social @tridentpct.bsky.social teams

#microtubules in orange hot, #F-actin greyscale, Fibroblast, spinning disc. @marcoenriquez18.bsky.social @aibnatuq.bsky.social #fluorescenceFriday

🔬 First live-cell imaging experiments done in my lab at HKU! Human RPE1 cells expressing Lifeact-GFP (actin) and EB3-GFP (MT plus-ends), imaged in Fast Airyscan mode on a Zeiss LSM 900👇. Thanks to Denise Kuok Iok Teng for imaging setup, and to Manuel Théry & Louise Bonnemay for the cells 🙏🫶😆

The high-speed logistics of a lung cancer cell. Watch GFP-Rab25 (Gold) move cargo through the internal recycling pathway of A549 cells. Static maps are great, but time-lapse shows the real traffic. Want your science/tech to be highlighted? DM me at Viroscope!

Our preprint on Antarctic fish cell cultures is out now! Loads of pretty nice super-resolution images. Read it here: www.biorxiv.org/content/10.6...

🚨 How do mechanical forces shape a developing organ? Our new #ScienceAdvances @science.org study, led by the amazing @cvagenapantoula.bsky.social, reveals a Piezo1-driven hydraulic mechanism, where mechanical cues control cell volume to guide cardiac formation ❤️🐟 🔗 www.science.org/doi/10.1126/...

New perspective on caveolae www.nature.com/articles/s41...

It's #FluorescenceFriday and these retinal astrocytes understood the assignment: branching, glowing, and casually laying down the blueprint for the retinal vasculature #Retina #ConfocalMicroscopy

For #FluorescenceFriday, an E10 embryo showing Pax2 localization. #DevelopmentalBiology

Happy #FluorescenceFriday! Just some random data I collected from a while back, actin and myosin in a U2OS cell imaged on our lattice light sheet microscope.

I am excited to share with you an amazing video on the NLRP3 inflammasome pathway generated by talented Ann Liu from the Janet Iwasa lab @jiwasa.bsky.social @ann-hl.bsky.social. It shows the real structures of the pathway from #NLRP3, #ASC, #caspase-1, #GSDMD, #IL-1b, to #NINJ1. Enjoy!

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

How does an ovarian follicle remodel in space and time to release an egg? 💥🥚 We have an exciting PhD project combining follicle culture, advanced imaging and quantitative analysis to understand the cellular dynamics of ovulation. 🔬 Co-supervised by me and @guignardlab.bsky.social. Please share! 🌱

1/ 🔬🧑‍💻 Tired of going back and forth between GUIs and code for your 3D image analysis needs? Would you like to keep your focus in one place (notebook), without sacrificing interactivity? Here’s a tool that can help!

Our latest work on shape-programmable tissues is out in @science.org. By positioning topological defects in cellular nematics, we encode frustrated 2D force fields that relax into predictable 3D shapes. Collaboration with Marino Arroyo’s lab, led by @pauguillamat.bsky.social at @ibecbarcelona.eu.

Mapping nerves in a whole embryos. We find that across species and development stages, embryonic nerves display (beautiful) fractal geometry. More here: www.biorxiv.org/content/10.6...

It's funky axons time 🔬 🧪

Can you guess why the microtubules are organized so differently at the bottom and top in this gigantic COS cell? (a game for you @manuelthery.bsky.social)

A sneak peak of a complex and technically challenging experiment that my lab developed: doi.org/10.1096/fj.2.... Super proud of PhD candidate Hannah Johnson for showcasing our Whole-gut spatial genomic analysis in #zebrafish. This video illustrates one landmark in the protocol after multiple 1/3

This developing quail embryo looks like it's thinking fiery thoughts for #FluorescenceFriday 🐣🔥🧪. Imaged by the fantastic @vanderspuy.bsky.social

The beauty of the kidney arterial tree for this #FluorescenceFriday. Evans Blue dye labels the vasculature. Image courtesy of postdoc Sarah McLarnon.

1/2 Happy #FluorescenceFriday 🔬 Another cryosection of a dissected zebrafish gill: ⚪Phalloidin 🔵DAPI

The first image I took of 2026 #FluorescentFriday

Latest work from the lab by Aude Nommick et al., in which we propose a "size-scaling" model for microtubule force exertion that regulates centrosome centration vs decentration during embryo development! @ijmonod.bsky.social www.biorxiv.org/content/10.6...

So many tuft cells (yellow/orange) for #TuftTuesday. Immune cells are in green.

Beautiful images from @christlet.bsky.social labelling tubulin in neurons #journalclub www.biorxiv.org/content/10.6...

A protein called Uninflatable acts as a switch between glial cells growing and glial cells wrapping themselves around axons. 🔗 buff.ly/WRdybO3

Excited to share Alison's @alisonkickuth.bsky.social paper from the lab out in @nature.com this week! We uncovered how a mechanical ratchet mechanism drives cytokinesis in early #zebrafish embryos. Read more in this thread 🧵 and at www.nature.com/articles/s41... 🤩 @poldresden.bsky.social @mpi-cbg.de

Diffusion first, Binding second Organ-scale uniform immunostaining in 1 DAY🤠, via temporally controlling antibody reaction strength CuRVE + eFLASH 🐭👤Brain 62 Ab tested 👉deoxycholic acid + pH 👉compatible with transgenic fluorescent proteins #NatBiotech 2025 www.nature.com/articles/s41...

New paper out! We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution. Huge congrats to Aurélie Favarin, Rayan Said, & all authors! In Science Advances: www.science.org/doi/10.1126/... #actin #myosin #mechanobiology

Hoping everyone's new year fluorescence is clear and bright. Happy #FluorescenceFriday.

New Perspective from myself, Sarah Heilbronner and @myoo.bsky.social . “Rethinking the centrality of brain areas in understanding functional organization” in Nature Neuroscience. 🧵 rdcu.be/eVZ1A

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

Felt a little festive at the microscope this morning for #FluorescenceFriday 🎄 Here’s the nervous system of a juvenile sea star ⭐️ Green = acetylated tubulin, red = nuclei Happy holidays!

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Network of somatosensory axons and immune cells in the adult zebrafish skin. Credit to @errricpeterman.bsky.social. #ZebrafishZunday 🧪

I am presenting at the Science and Art Minisymposium at #CellBio2025 today! The line up is great this year. I am hands down the least qualified person presenting. It is a weird feeling but I am excited! Here is my title slide. Room 122 4pm