Has anyone here worked with Laurdan in mammalian cells? I recently labelled some cells and see no signal with 405 nm excitation, but I do see lysosome-like structures when exciting at 488 nm. I also co-stained with LysoTracker and most of the signal overlaps. Any thoughts?

The #MSCA results are out and the overall success rate is 9.6%. My friend with 96.8 score is in the reserve list! Interesting!

Haven’t taken a flight in 14 months ✈️ Won’t take one until April either, which’ll make it 17. Just a reminder that travel choices do add up for CO₂. One person skipping a flight doesn’t ground a plane, but lower demand over time does mean fewer routes and frequencies.

Crafting New Year (super-)resolutions at cold for 2026 Happy New Year everyone 🎉

Merry Christmas to all our students, staff, alumni and friends around the world 🎄 📸 Lloyd Mann

Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read! Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase. www.biorxiv.org/content/10.6... Happy holidays ✨🎄

Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social

Nice collection of cameras!

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.

Tracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff: Paper: doi.org/10.1242/jcs.... Code: github.com/pertzlab/arc... Plugin: github.com/pertzlab/arc...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...

My shirt is cooler than yours

Fantastic night at Christ’s college for the LAG-MNG Christmas meal! @lasergroup.bsky.social

I won’t buy a lens from you after seeing you put it upside down. 🙃

Nature’s optics at its best! A full lunar halo lighting up the night.

Another huge advantage of miOPM is that it's gentle! A light-sheet microscope that's compatible with standard slides and multi-well plates! -> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely! doi.org/10.1101/2025...

PhD studentship available on using our single-molecule flow cytometry technology to detect rare proteins. Apply if you're interested in applying single-molecule techniques to improve diagnostics and to discover new routes for anti-cancer immunotherapy. www.findaphd.com/phds/project...

Always there for our group meetings!

Single-molecule friends! The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec... It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June: Have a look, tell your friends, and register!

Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries. doi.org/10.1101/2025...

👀 What if your flow cytometer could see the invisible? Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry. www.biorxiv.org/content/10.1...

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

I was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?

The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

📽️ G&D Tapes 📽️ G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social Read the full story here: ➡️ tinyurl.com/gd352899

Excellent choice of photo to begin the talk! Speaker: Dr Meng Lu from Peking University

Check out our latest preprint, which is the latest production from our long-lasting collaboration with Andrey Klymchenko > functionnalizing NPs with humanized antibodies (i.e. Trastuzumab). Work performed by @vmittelheisser.bsky.social & O.Lefebvre in my lab. www.biorxiv.org/content/10.1...

They showed how the conformational dynamics of single molecules can be retrieved from FRET correlation functions in diffusion-based smFRET experiments. www.nature.com/articles/s41...

Slanted light-sheet array microscopy for large volume imaging at rates exceeding 100 Hz arxiv.org/pdf/2506.13664

A review on cytoplasm to explore how cytoplasmic organisation contributes to metabolic regulation! pubs.acs.org/doi/full/10....

Transforming iterative MINFLUX from a precision localisation tool into a real-time SPT method.

Beautiful liquid-liquid phase separation story! elifesciences.org/articles/96722

Could we create a platform for tracking analysis that all these new, cool tools could be on? www.biorxiv.org/content/10.1...

Claim to deliver 10 nm in xy an 25 nm in z loc precision (no FRC provided?). Photon-efficient and 100x faster (!), compared to what? www.biorxiv.org/content/10.1...

Silicon-assisted interferometric Localization Microscopy (SiLM) Clever chip engineering for interferometric SMLM. It sounds an alternative if you can’t build 4Pi/iPALM. Would love to see some FRC-based resolution comparisons to really benchmark it. www.biorxiv.org/content/10.1...

SMLM shows us how close molecules are, but just because two molecules are close doesn’t mean they’re bound. This new probabilistic framework changes that. They can actually quantify interactions at the single-molecule level. Fascinating! www.pnas.org/doi/abs/10.1...

Need to play more with it but so far works great on this OPM data.

SOALSI (Single-Objective Airy Light-Sheet Imaging) pubs.acs.org/doi/full/10....

from optiland import analysis from optiland.samples.objectives import CookeTriplet lens = CookeTriplet() lens.draw()

We present: A deconvolution solution to aliasing confusion in apparently undersampled oblique plane microscopy doi.org/10.1101/2025... [1/8]

#ImageScanningMicroscopy inherently encodes aberration info. No extra hardware needed! A #CNN decodes Zernike modes directly from a single acquisition. A new data-driven path to #AdaptiveOptics in #LSM! doi.org/10.1364/BOE.... #PhotonResolvedMicroscopy #SPADarray @iitalk.bsky.social @erc.europa.eu

🚨 Join us in shaping the future of #SmartMicroscopy! We're hiring PhD & fellowship positions at the intersection of #photonics, electronics & #AI. Check out our works in this field doi.org/10.1038/s414... www.nature.com/articles/s41... doi.org/10.1101/2024... Please share! #SPADarray #Microscopy

👀 www.researchsquare.com/article/rs-6...

#FluorescenceFriday contribution: combined single-molecule FRET and single-particle tracking of the MET receptor tyrosine kinase in living cells reports on dimer lifetime (around one second), diffusion mode of monomer vs. dimer, and more (soon to come and at #FOM2025).

A DIY high-resolution light-sheet microscope! Congrats to @john-haug.bsky.social, who simulated, designed, built, and validated the system! Resolution: 235 nm (x), 233 nm (y), 350 nm (z). On par with LLSM, but easier to build. 🔥 www.biorxiv.org/content/10.1... #LightSheet #Microscopy