Copy our protocols! 📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22... 🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc... #OpenScienceWeek

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

Why hasn't Preprint Peer Review caught on more? I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs. more in thread below!

We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...

"proliferation of LLMs has prompted many people without experience to start creating [screening] tools...Simpler approaches often perform better [and] LLM results may not be reproducible...The fact that you can use an LLM doesn’t mean that you should." www.arxiv.org/abs/2507.17991 •

We just published: Why you should sequence your plasmids (and how to do it for free) Through @GetGenome scientists in 100 countries can access free whole-plasmid sequencing —giving researchers the confidence to move their experiments forward. medium.com/p/why-you-sh...

There's no evidence that opossums eat any ticks, but it's a common myth (www.sciencedirect.com/science/arti...). I asked ChatGPT & Copilot, "Do opossums eat ticks?" Both confidently: "yes, thousands per week!". I then prompted, "Isn't it a myth?" And both answered: "yes, it's been debunked!"

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

How do we correct preprints? Caveat emptor, revisions & withdrawals - what this means for the scientific record. My presentation to the National Academies 1/n vimeo.com/1102568612?s...

So, Elon Musk, a Silicon Valley technocrat, created DOGE with the supposed goal of increased government efficiency. But what we got instead is an extra government entity that has inserted senseless chaos and waste and worsened the existing bureaucracy?

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

The U.S. has led the world in science since the mid-20th century for two reasons: funding & welcoming students and researchers from around the world. The Trump administration is destroying both advantages (1/2)

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...

Happy to answer any and all questions about this work. We'd love to see more people try it. Full protocol: www.protocols.io/view/craftse... Full analysis: github.com/immunogenomi...

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org[email protected] papers. www.protocols.io/view/re-usin... www.protocols.io/view/washing...

Comment on our @Reproducibility project in @Drosophila immunity. It is a normal that findings—however exciting and important at the time—may later prove to be more complex than initially believed. Re-evaluations is essential to the self-correcting nature of science. www.science.org/content/arti...

The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪 dx.doi.org/10.17504/pro...

🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let... The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!

Last month we published our protocol for detection of beet yellows virus. Here it is! RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...

At @sheffielduni.bsky.social we're strongly pro-openness, but alas not when it comes to pigeons...

qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io

Not sure why I'm thinking of this today.

This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠 🔗 Check them out: www.protocols.io/view/behavio...

Today. bsky.app/profile/ltey...

Tomorrow: webinar with @ruthtimme.bsky.social, "Improving Global Pathogen Surveillance: Leveraging Open Science Through Standardized Protocols". go.springernature.com/improving-gl...

🥼 Do you want to start working with human pluripotent stem cells? 📖 Check out our protocols collection "Good practices in hPSC handling", developed among the CorEuStem COST Action Project at COST Association #stemcells www.protocols.io/view/good-pr...

Interested in studying Hydra? Our lab researcher Kelso Cochran wrote a comprehensive protocol (used to train all of our undergrads) now shared on protocols io on how to get started www.protocols.io/view/compreh...

🏅 Shining a light on exceptional Life Science Departments! 🏅 Know a team excelling in: 📄 Preprint practices ✏️ Open peer reviews 🌐 Transparent research sharing? Nominate them to be featured in our case studies on academic brilliance! docs.google.com/forms/u/1/d/...

The protocols from Protocol Exchange are now live on protocols.io, expanding the @protocolsio.bsky.social repository from 21,000 to 23,000 public protocols. www.protocols.io/blog/protoco...

🔬 Are you working in core facilities, research infrastructures, or technology platforms? Don't miss the CTLS2025 – Core Technologies for Life Sciences Congress, taking place 10–12 June 2025 in Brno, Czech Republic!

Are you a researcher who'd like to know more about creating open methods & protocols? Can you be in London 2nd June am? Then come to the training event that the Crick & protocols.io are putting on for the Open Science & Scholarship Festival. Register here: forms.office.com/e/EecPjyU1UU

Looking forward to participating in this webinar tomorrow! bsky.app/profile/prot...

Let's not forget that the war on science isn't a Trump anomaly; it's a sustained long-term effort of the Republican Party. I was shocked in 2015 when I looked at the official Twitter feed of the US House Committee on Science, Space & Tech. web.archive.org/web/20160203... 1/3

For the last 3 yrs, I was the director for the Science of Science program at the NSF. We funded projects on science communication - science communication to the public, communication of public priorities to scientists, citizens engagement & participation in science. 🧵

"From Lab to Field - Advancing eDNA Research with protocols.io" (Webinar on Wednesday, April 30th, 2pm EST) communities.springernature.com/posts/upcomi...

🧠🧬 We still don’t know how antidepressants *actually* work. Why does depression respond to treatments as diverse as SSRIs, TCAs, electroconvulsive therapy, TMS, tianeptine, and ketamine? What do these treatments have in common? (hint: not serotonin) See our new preprint! doi.org/10.1101/2025...

Thrilled to share the new & open-access "VERA 2.0" method and its experimental validation: a semi-automated, fast, and reliable protocol for reconstructing past physical activity from skeletal remains! Validation paper: doi.org/10.1371/jour... Protocol: protocols.io/view/protoco...

Spotlight interview with Fernando Lopes and Conrad Gillett regarding their PLOS ONE Lab Protocol paper: "From museum drawer to tree: Historical DNA phylogenomics clarifies the systematics of rare dung beetles (Coleoptera: Scarabaeinae) from museum collections."

There is a lot I disagree with here but perhaps this most of all: “I’ve interviewed dozens of scientists who don’t know how to evaluate preprints, what they can trust, or what they can use in their own research.”

incredible. I guess according to the author all of physics has been serving an "anti-science agenda" since the 90s...

I frequently disagree with S-Kitch posts, but I don't even know where to start with this one...Are Maths & Physics a hot bed of anti-science? I haven't noticed! The current 2 or 3 ppl peer review doesn't exactly guarantee credibility & you can just keep trying journals until somewhere publishes it.

🍵Read our April issue Editorial on how integrating protocols.io into the submission process makes collaborative protocol sharing and peer review seamless for authors and referees in a step towards open science. 👉https://rdcu.be/eicaY bit.ly/42hNWk3

An important point: If you share your protocol in a preprint server or in protocols repository like protocols.io you can still publish your protocol in a peer-reviewed journal like Nature Protocols at a later date.

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

I think this is the first time since the inauguration that I laughed out loud at a political joke. theonion.com/teen-warned-...

Looking for advice: we had trouble with long read DNA extraction for PacBio sequencing, from ethanol preserved samples. Is ethanol a general problem for this, and can anyone suggest alternatives?

Pleased to see another new open protocol coming from the Gandhi lab at the Crick today. www.protocols.io/view/crispri...

On a personal curiosity and somewhat professional level, I'd love to hear from anyone that wants to try RT&Tag or has tried RT&Tag! 👀 Reply here or shoot me a message! Nadiya has a wonderful protocols.io page for the original RT&Tag method: www.protocols.io/view/rt-amp-...

Following the $400 million fund cut at Columbia and $800 million at Johns Hopkins, the U.S. Department of Education released a list of 58 additional universities which may face similar "punishment", from UC-Berkeley to Harvard to UNC-Raleigh. This is quite the list:

Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's not a new preprint server; it's dedicated organization to oversee the servers] openrxiv.org 1/n