Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online go.glam.ox.ac.uk/proto

Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase! dx.doi.org/10.17504/protocols.io.261ged7xov47/v2 Thank you @jcoker10.bsky.social and Michael Lim!

We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently. If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! 😀 www.protocols.io/organization...

ONT seq help: Has anyone had success using less than the 10ng of input DNA for the 16S barcoding kit? Trying to use it for low biomass samples.

Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response: IPC: www.protocols.io/view/sequenc... icddr,b/CDC: journals.asm.org/doi/10.1128/...

New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat... With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.social

Join Springer Nature editors and the protocols.io team for an exciting event as part of #LoveMethods26! 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22. Our editor Sabrya Carim will be there! 👉FREE registration & more details: www.protocols.io/blog/join-pr...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:

You want to take sediment samples from archaeological sites for a variety of analysis?Have a look at our best practise sampling protocol: www.protocols.io/view/samplin...

Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626

Some EBP-affiliates are embarking on ambitious R&D to tackle some of the hardest genomes on Earth. 🌍🧬 Great science starts with shared ideas — sometimes over coffee. 💥🤝☕️ Soak up the Sanger Tree of Life sponge protocol 👇 www.protocols.io/view/sanger-... @seagrassliz.bsky.social #Biodiversity

A new Lab Protocol from @sabass-lab.bsky.social and colleagues presents an in vitro rumen culture and workflow that reproducibly screens anti-methanogenic compounds, aiding feed-based methane mitigation studies: plos.io/4oyyc4a Protocol available via @protocolsio.bsky.social at plos.io/3KAOThi

Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost. 🌟 Full Springer Nature press release: group.springernature.com/gp/group/med... #OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio

It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...

We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...

Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!

With the full CRISPR 7-11 protocol here :)

Link for the full CRISPR Cas7-11 protocol: www.protocols.io/view/crispr-...

Congratulations to @sofiram.bsky.social and @artemiskorovesi.bsky.social on our last paper about the role of Sox8 in #xenopus #gastrulation A link to the whole paper is here www.embopress.org/doi/full/10....

Excited to launch an openRxiv partnership with the scientist-run AI review service qed (@qedscience.bsky.social), the brainchild of @odedrechavi.bsky.social 1/n openrxiv.org/enabling-rev...

Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now: www.medrxiv.org/content/10.1... ✅ Now implemented in 6 NHS hospitals ✅ Increased sensitivity ✅ Increased pathogen genome coverage (1/3)

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

✨ Research assessment needs a rethink — and it finally feels like change might be possible. At protocols.io (@protocolsio.bsky.social) and in my past life as a journal editor, I’ve had countless conversations about how we evaluate researchers. It’s a topic that keeps coming up — and for good reason.

Explore our new featured protocols protocols.io/welcome

In which I look at animal research for The New York Times and find some surprising answers.

Listening to #WhatTheRef podcast on the train en route to Birmingham for The Festival of Hidden Ref tomorrow and Weds, perfect listening material, thanks @hiddenref.bsky.social, I'm giving a lightning talk on Thurs - very much looking forward to this event :)

I've been dreaming about this for a decade and it's now live! "Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io" www.protocols.io/blog/global-...

Copy our protocols! 📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22... 🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc... #OpenScienceWeek

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

Why hasn't Preprint Peer Review caught on more? I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs. more in thread below!

We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...

"proliferation of LLMs has prompted many people without experience to start creating [screening] tools...Simpler approaches often perform better [and] LLM results may not be reproducible...The fact that you can use an LLM doesn’t mean that you should." www.arxiv.org/abs/2507.17991 •

We just published: Why you should sequence your plasmids (and how to do it for free) Through @GetGenome scientists in 100 countries can access free whole-plasmid sequencing —giving researchers the confidence to move their experiments forward. medium.com/p/why-you-sh...

There's no evidence that opossums eat any ticks, but it's a common myth (www.sciencedirect.com/science/arti...). I asked ChatGPT & Copilot, "Do opossums eat ticks?" Both confidently: "yes, thousands per week!". I then prompted, "Isn't it a myth?" And both answered: "yes, it's been debunked!"

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

How do we correct preprints? Caveat emptor, revisions & withdrawals - what this means for the scientific record. My presentation to the National Academies 1/n vimeo.com/1102568612?s...

So, Elon Musk, a Silicon Valley technocrat, created DOGE with the supposed goal of increased government efficiency. But what we got instead is an extra government entity that has inserted senseless chaos and waste and worsened the existing bureaucracy?

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

The U.S. has led the world in science since the mid-20th century for two reasons: funding & welcoming students and researchers from around the world. The Trump administration is destroying both advantages (1/2)

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...

Happy to answer any and all questions about this work. We'd love to see more people try it. Full protocol: www.protocols.io/view/craftse... Full analysis: github.com/immunogenomi...

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org[email protected] papers. www.protocols.io/view/re-usin... www.protocols.io/view/washing...

Comment on our @Reproducibility project in @Drosophila immunity. It is a normal that findings—however exciting and important at the time—may later prove to be more complex than initially believed. Re-evaluations is essential to the self-correcting nature of science. www.science.org/content/arti...

The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪 dx.doi.org/10.17504/pro...

🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let... The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!

Last month we published our protocol for detection of beet yellows virus. Here it is! RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...

At @sheffielduni.bsky.social we're strongly pro-openness, but alas not when it comes to pigeons...

qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io

Not sure why I'm thinking of this today.

This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠 🔗 Check them out: www.protocols.io/view/behavio...