👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio! Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉 ➡️ Mouse Perfusions (bit.ly/4qH6rIY) ➡️ Y-Maze (bit.ly/47aITTC) ➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)

Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now: www.medrxiv.org/content/10.1... ✅ Now implemented in 6 NHS hospitals ✅ Increased sensitivity ✅ Increased pathogen genome coverage (1/3)

Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF: 🔗 www.researchprofessionalnews.com/rr-news-uk-r... Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.

Explore our new featured protocols protocols.io/welcome

I've been dreaming about this for a decade and it's now live! "Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io" www.protocols.io/blog/global-...

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

Copy our protocols! 📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22... 🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc... #OpenScienceWeek

We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org[email protected] papers. www.protocols.io/view/re-usin... www.protocols.io/view/washing...

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...

Happy to answer any and all questions about this work. We'd love to see more people try it. Full protocol: www.protocols.io/view/craftse... Full analysis: github.com/immunogenomi...

I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression. www.nature.com/articles/s41...

The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪 dx.doi.org/10.17504/pro...

🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let... The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!

Last month we published our protocol for detection of beet yellows virus. Here it is! RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...

qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io

Happy to be in Brno for the #CTLS2025 conference from the Core Technologies for Life Sciences society. I'll be talking about protocols.io tomorrow morning in the Core Facilities and Open Science session

Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats." @realdiehllab.bsky.social

For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes! scientistseessquirre...

A fantastic start to #OSFest25 this morning, looking at publishing methods and protocols with @protocolsio.bsky.social. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place: buff.ly/jZsSd6U

This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠 🔗 Check them out: www.protocols.io/view/behavio...

Today. bsky.app/profile/ltey...

🥼 Do you want to start working with human pluripotent stem cells? 📖 Check out our protocols collection "Good practices in hPSC handling", developed among the CorEuStem COST Action Project at COST Association #stemcells www.protocols.io/view/good-pr...

Talking about training and opportunities for technicians around publishing. We're trying to get ours interested in using protocols.io to share work they're already doing optimising protocols. #DARTS9

Why so detailed? I did Hydra care as a postdoc and when I tried to set up our lab Hydra it went horribly! After joining our lab, Kelso looked through multiple protocols and did some troubleshooting until our lab populations were thriving.

Interested in studying Hydra? Our lab researcher Kelso Cochran wrote a comprehensive protocol (used to train all of our undergrads) now shared on protocols io on how to get started www.protocols.io/view/compreh...

The protocols from Protocol Exchange are now live on protocols.io, expanding the @protocolsio.bsky.social repository from 21,000 to 23,000 public protocols. www.protocols.io/blog/protoco...

Spotlight interview with Fotios Alexandros Karakostis regarding his PLOS ONE Lab Protocol paper: "Introducing “Validated entheses-Based reconstruction of activity 2.0” (VERA 2.0): Semi-automated 3D analysis of bone surface changes."

You've heard about the upcoming festival, but let's take a look at some of the individual sessions! The first event on the programme is taking place in person at @crick.ac.uk and will be featuring experts from @protocolsio.bsky.social. The workshop is open to all.

Spotlight interview with Daniel Foulkes regarding his PLOS ONE Lab Protocol paper: "Stable topical application of antimicrobials using plumbing rings in an ex vivo porcine corneal infection model."

When publishing the first benchmarking data for a new sequencing technology like CycloneSeq, it's great having @protocolsio.bsky.social to share the protocols in a transparent manner. CycloneSEQ Library Construction and Sequencing Protocol for Isolated Bacteria. dx.doi.org/10.17504/pro...

Are you a researcher who'd like to know more about creating open methods & protocols? Can you be in London 2nd June am? Then come to the training event that the Crick & protocols.io are putting on for the Open Science & Scholarship Festival. Register here: forms.office.com/e/EecPjyU1UU

Spotlight interview with Elisa Crisci regarding her PLOS ONE Lab Protocol paper: "High-throughput 96-well plate-based porcine antibody isolation protocol."

I'm late to the party, but here's a 🦋 intro! I'm a postdoc at Stanford studying how the hippocampal circuit supports multiple aspects of spatial memory and how it goes awry in aging and #Alzheimers. Starting my own lab soon 👀 👉 www.biorxiv.org/content/10.1... 👉 www.protocols.io/view/chronic...

"From Lab to Field - Advancing eDNA Research with protocols.io" (Webinar on Wednesday, April 30th, 2pm EST) communities.springernature.com/posts/upcomi...

"From Lab to Field - Advancing eDNA Research with protocols.io" (Webinar on Wednesday, April 30th, 2pm EST)

🧠🧬 This work was conducted by Eva Geoghegan as part of the Brain Data Alchemy Project, within a collaboration between the Wakil lab (@huda-akil.bsky.social) at Univ. of Michigan (@ummni.bsky.social) and the Hen lab at Columbia University (@phi-nguyen.bsky.social). www.protocols.io/view/brain-d...

🧠🧬 We still don’t know how antidepressants *actually* work. Why does depression respond to treatments as diverse as SSRIs, TCAs, electroconvulsive therapy, TMS, tianeptine, and ketamine? What do these treatments have in common? (hint: not serotonin) See our new preprint! doi.org/10.1101/2025...

Thrilled to share the new & open-access "VERA 2.0" method and its experimental validation: a semi-automated, fast, and reliable protocol for reconstructing past physical activity from skeletal remains! Validation paper: doi.org/10.1371/jour... Protocol: protocols.io/view/protoco...

Spotlight interview with Fernando Lopes and Conrad Gillett regarding their PLOS ONE Lab Protocol paper: "From museum drawer to tree: Historical DNA phylogenomics clarifies the systematics of rare dung beetles (Coleoptera: Scarabaeinae) from museum collections."

🍵Read our April issue Editorial on how integrating protocols.io into the submission process makes collaborative protocol sharing and peer review seamless for authors and referees in a step towards open science. 👉https://rdcu.be/eicaY bit.ly/42hNWk3

📝 Paper: www.nature.com/articles/s41... 1/5. 🚂 MetaFlowTrain = 4 modular components: a. Variety of liquid inputs b. 24-channel pump enabling parallel metabolic trains c. 3D-printed gnotobiotic microchambers d. Multi-layered outputs for cell/metabolite analysis 🔗 protocols.io/view/36wgqd68ovk5/v3

🚨Excited to share our new publication! MetaFlowTrain, a highly parallelized and modular fluidic system to study exometabolite interactions between microbes-microbes-plants 🌱 @hacquardst.bsky.social @herpell.bsky.social S.Wolf S.Perin @mpipz.bsky.social www.youtube.com/watch?v=gNUf... 🧵Thread 5/5:

An important point: If you share your protocol in a preprint server or in protocols repository like protocols.io you can still publish your protocol in a peer-reviewed journal like Nature Protocols at a later date.

Protocol Spotlight interview with Giuseppe Grasso regarding his PLOS ONE Lab Protocol paper: "Label-free determination of diffusion coefficients at the nanoscale through modelling of the Surface Plasmon Resonance signal."

Protocol Spotlight interview with Brittany Walters regarding her PLOS ONE Lab Protocol paper: "Longitudinal imaging of the taste bud in vivo with two-photon laser scanning microscopy"

On a personal curiosity and somewhat professional level, I'd love to hear from anyone that wants to try RT&Tag or has tried RT&Tag! 👀 Reply here or shoot me a message! Nadiya has a wonderful protocols.io page for the original RT&Tag method: www.protocols.io/view/rt-amp-...