www.protocols.io
@protocolsio.bsky.social
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📥 3
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A secure platform for developing and sharing reproducible methods.
We’re transforming research protocols from static documents into dynamic, reusable workflows. A key part is making large collections of trusted methods — including Protocol Exchange and Springer Protocols — available in
protocols.io
format, so researchers can reuse, adapt, and build on them.
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Evolving protocols: From static to dynamic with protocols.io
Sharing research protocols is a cornerstone of open science, and protocols.io, part of Springer Nature, continues to evolve as a secure, modern platform for...
https://www.protocols.io/blog/evolving-protocols-from-static-to-dynamic-with
3 days ago
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Springer Nature
27 days ago
Strengthening transparency and reproducibility starts with sharing research work openly. Our latest blogs offer guidance for: > How to share research code openly:
spklr.io/63329Eynzz
> How to share research protocols and methods openly:
spklr.io/63322EynzO
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Attending ABRF 2026 (
@abrf.bsky.social
)? Connect with
protocols.io
at Booth 710. We're engaging with core facilities about common priorities and challenges, including how to demonstrate impact and receive credit. If this resonates, schedule a short discussion with our team:
abrf26.youcanbook.me
about 1 month ago
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Noura Maziak
about 2 months ago
Excited to share my PhD work! The question: How is genome organization established? Here, we developed a low-input Micro-C method, Pico-C, and used it to map chromatin architecture across early stages of fly development. Here’s a quick Blue-torial 🙂 (1/n)
www.nature.com/articles/s41...
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Three-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila - Nature Genetics
Pico-C, a low-input Micro-C approach, reveals that dynamic three-dimensional genome folding precedes zygotic genome activation in Drosophila.
https://www.nature.com/articles/s41588-026-02503-3
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Join our webinar to hear from researchers at the University of Rochester and City of Hope on practical strategies for improving documentation, supporting training, and increasing visibility of core facility work. April 9 | 11 AM EST | 5 PM CET Register here:
go.springernature.com/enhancing-im...
about 2 months ago
0
1
1
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www.protocols.io
Radcliffe Science Library
about 2 months ago
Last month, we highlighted
Protocols.io
as a new resource available at
@bodleian.ox.ac.uk
. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online
go.glam.ox.ac.uk/proto
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www.protocols.io
Kelvin Lau @klausenhauser on 🙅♂️🐘🪡
2 months ago
Started this project years ago, but now everything is deposited on
@addgene.bsky.social
and a short
@protocolsio.bsky.social
is online. Try it out to make your own (cheap) homemade nuclease/Benzonase!
dx.doi.org/10.17504/protocols.io.261ged7xov47/v2
Thank you
@jcoker10.bsky.social
and Michael Lim!
3
32
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www.protocols.io
Francis Crick Institute Library & Information Services
2 months ago
We've updated our
protocols.io
page to highlight a couple of the protocols published by Crick authors recently. If you're at the Crick, don't forget you can get your flat pdfs converted to the
protocols.io
format at no cost! 😀
www.protocols.io/organization...
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Francis Crick Institute
The Francis Crick Institute (formerly the UK Centre for Medical Research and Innovation) is a biomedical research centre in London, which was established in 2010 and opened in 2...
https://www.protocols.io/organizations/francis-crick-institute
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www.protocols.io
PLOS One
2 months ago
A Lab Protocol by Omid Gholamalamdari and Stephanie C. Weber details an optimized method for labeling of nascent RNA transcripts in the C. elegans intestine, enabling spatial analysis of transcription dynamics.
plos.io/3Ox4rnO
@protocolsio.bsky.social
link:
plos.io/3O9Jv6g
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Labeling of nascent RNA in the C. elegans intestine
Transcriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivo…
https://plos.io/3Ox4rnO
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5
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www.protocols.io
Daniel Bolnick
3 months ago
New
@protocolsio.bsky.social
post describing our new protocol for generating intestinal organoids for
#stickleback
:
www.protocols.io/view/generat...
With work by postdoc Arshad Padhiar, funded by
@moorefound.bsky.social
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Generation of Stickleback intestinal organoid
Intestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin...
https://www.protocols.io/view/generation-of-stickleback-intestinal-organoid-bp2l6e3pzgqe/v1
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www.protocols.io
Sara Zufan, PhD, MPH
3 months ago
Given the recent
#Nipah
outbreak in India, here are two
#Nanopore
protocols for NiV genomic sequencing in support of rapid outbreak response: IPC:
www.protocols.io/view/sequenc...
icddr,b/CDC:
journals.asm.org/doi/10.1128/...
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www.protocols.io
Springer Nature
3 months ago
💡 Did you know? Methods are among the most cited research outputs. For #LoveMethods26, join experts for interactive sessions: 👉 Jan 21: @protocolsio.bsky.social Hackathon 👉 Jan 22: Methods Mythbusting Panel
spklr.io/633288eAVM
#LoveMethods26
#OpenScience
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www.protocols.io
Francis Crick Institute Library & Information Services
3 months ago
Happy International Love Methods week! The Crick partners with
@protocolsio.bsky.social
to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!)
www.protocols.io/we-enter-pro...
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We will import and format your protocol
Paper protocols floating on your benchtops? We can digitize them for you!
https://www.protocols.io/we-enter-protocols
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www.protocols.io
Join Springer Nature editors and the
protocols.io
team for two exciting events as part of
#LoveMethods26
! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:
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Join protocols.io and Springer Nature editors at Love Methods Week 2026
Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...
https://www.protocols.io/blog/join-protocolsio-and-springer-nature-editors-at
4 months ago
0
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Join Springer Nature editors and the
protocols.io
team for two exciting events as part of
#LoveMethods26
! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:
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Join protocols.io and Springer Nature editors at Love Methods Week 2026
Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...
https://www.protocols.io/blog/join-protocolsio-and-springer-nature-editors-at
4 months ago
0
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5
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Radcliffe Science Library
4 months ago
Our promoted new resource for January is
Protocols.io
. Using
Protocols.io
researchers can create and share detailed experimental protocols with colleagues or the wider scientific community
www.protocols.io
0
3
1
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www.protocols.io
Nature Protocols
4 months ago
Join us for “Methods Mythbusting: Insights from Journal Editors and
protocols.io”
protocols.io/webinars/met...
rs at Love Methods Week 2026
excelscior.uc.pt/love-methods...
6/
#LoveMethods26
26
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Methods Mythbusting: Insights from Journal Editors and protocols.io
This webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H...
https://protocols.io/webinars/methods-mythbusting-insights-from-journal-editors
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www.protocols.io
GenomiqueENS
5 months ago
🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on
protocol.io
.
dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1
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https://protocol.io
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GenomiqueENS
5 months ago
🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔
1
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www.protocols.io
Jacques Dainat
5 months ago
Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!!
www.protocols.io/view/structu...
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2
1
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www.protocols.io
Emma Ganley
5 months ago
Exciting news: Premium
protocols.io
is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost. 🌟 Full Springer Nature press release:
group.springernature.com/gp/group/med...
#OpenScience
#Reproducibility
#GlobalCollaboration
#Research4Life
#protocolsio
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New collaboration empowers thousands of researchers to share research methods, improve reproducibility and drive global scientific progress | Springer Nature Group | Springer Nature
Heidelberg | London, 27 November 2025 Springer Nature and Research4Life extend partnership to include access to protocols.io for scientists in over
https://group.springernature.com/gp/group/media/press-releases/extended-partnership-with-research4life-to-protocols-io/27831376
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Springer Nature
5 months ago
Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social. Learn more:
group.springernature.com/gp/group/med...
#Research
🧪
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www.protocols.io
Lenny Teytelman
5 months ago
Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub
librehub.github.io
) is an amazing initiative from
@makertobey.bsky.social
and colleagues!
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The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub)
...
https://librehub.github.io
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Aureliano Bombarely
5 months ago
We have done our first
@protocolsio.bsky.social
. Are you interested about
#genome_annotation
quality evaluation? We have developed a tool called
#GAQET2
(
github.com/victorgcb198...
) which runs several tools like
#AGAT
,
#BUSCO
, and many other tools. You can find it at
www.protocols.io/view/structu...
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GitHub - victorgcb1987/GAQET2
Contribute to victorgcb1987/GAQET2 development by creating an account on GitHub.
https://github.com/victorgcb1987/GAQET2
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www.protocols.io
Tobias Wenzel
5 months ago
It was great to hear from
@lteytelman.bsky.social
about
Protocols.io
today for our LIBRE hub channel on open science! We already have a tutorial in Spanish:
youtu.be/RuBKWouEyL8?...
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Construyendo protocolos científicos reproducibles y abiertos con Protocols.io
YouTube video by LIBRE hub
https://youtu.be/RuBKWouEyL8?si=KtQfNUXTrU0EIiN2
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www.protocols.io
Barriga Lab
6 months ago
With the full CRISPR 7-11 protocol here :)
add a skeleton here at some point
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www.protocols.io
Aligning Science Across Parkinson's
6 months ago
👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio! Their open-access protocols ranked among the top 100 most viewed on
@protocolsio.bsky.social
in August 🎉 ➡️ Mouse Perfusions (
bit.ly/4qH6rIY
) ➡️ Y-Maze (
bit.ly/47aITTC
) ➡️ Culture/transfection of HEK293T cells (
bit.ly/4o4ytfR
)
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www.protocols.io
Ronan Doyle
6 months ago
Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:
www.medrxiv.org/content/10.1...
✅ Now implemented in 6 NHS hospitals ✅ Increased sensitivity ✅ Increased pathogen genome coverage (1/3)
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Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays
Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...
https://www.medrxiv.org/content/10.1101/2025.10.10.25337716v1
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www.protocols.io
Emma Ganley
7 months ago
Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF: 🔗
www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
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‘REF needs dedicated section for research methods’ - Research Professional News
Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework
https://www.researchprofessionalnews.com/rr-news-uk-research-councils-2025-10-ref-needs-dedicated-section-for-research-methods/
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Explore our new featured protocols
protocols.io/welcome
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Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
http://protocols.io/welcome
7 months ago
1
4
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reposted by
www.protocols.io
Lenny Teytelman
7 months ago
I've been dreaming about this for a decade and it's now live! "Global Collaboration Made Easier: Multilingual Protocols Now Available on
protocols.io"
www.protocols.io/blog/global-...
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Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io
Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...
https://www.protocols.io/blog/global-collaboration-made-easier-multilingual
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www.protocols.io
Michael Meers
7 months ago
We published a detailed protocol for our Plate-CUT&Tag method on
@protocolsio.bsky.social
to accompany our recent preprint (
www.biorxiv.org/content/10.1...
) give it a try! Feedback welcome!
dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1
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Plate CUT&Tag (Meers Lab version)
This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...
https://dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1
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allen institute
7 months ago
Copy our protocols! 📝 Find many of our lab protocols and techniques on
@protocolsio.bsky.social
at
www.protocols.io/search?q=%22...
🎥 Go deeper with recorded video tutorials of some of our most requested methods:
alleninstitute.org/education/sc...
#OpenScienceWeek
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www.protocols.io
Emma Ganley
8 months ago
We're hiring at
protocols.io
(
@protocolsio.bsky.social
) in NY or Jersey City - Associate Scientific Engagement Manager See LinkedIn post for more info & feel free to message me:
www.linkedin.com/posts/eganle...
0
5
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reposted by
www.protocols.io
Magnetic Resonance News
9 months ago
Protocols
@nmrfam.bsky.social
Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2
www.protocols.io/view/network...
#NMRchat
#NMR
🧲
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Network for Advanced NMR Materials SSNMR Data Acquisition Protocols
The NAN Materials SSNMR Data Acquisition Protocols collection is part of the NAN Materials SSNMR Knowledgebase; a collection of resources, training and educational material, pul...
https://www.protocols.io/view/network-for-advanced-nmr-materials-ssnmr-data-acqu-dm6gp97epvzp/v2
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www.protocols.io
BaglaenkoLab
9 months ago
Author here to answer any questions. The full protocol is online at
protocols.io
and all codebooks on github
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Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
https://protocols.io
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www.protocols.io
Anya Suppermpool
9 months ago
Load gels? Use
#zebrafish
🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our
@science.org
[email protected]
papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...
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Re-using pipette tips for gel loading
Gel loading tips can be reused for most applications; there’s REALLY no need to throw them away after a single use. In our labs, we routinely reuse tips when loading gels, onl...
https://www.protocols.io/view/re-using-pipette-tips-for-gel-loading-g5fnby3mf
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www.protocols.io
Yi Yang
9 months ago
Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation
www.protocols.io/view/compara...
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Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Bas...
Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the tru...
https://www.protocols.io/view/comparative-analysis-of-phage-profiles-in-environm-g4g2bytyf
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www.protocols.io
BaglaenkoLab
9 months ago
Happy to answer any and all questions about this work. We'd love to see more people try it. Full protocol:
www.protocols.io/view/craftse...
Full analysis:
github.com/immunogenomi...
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CRAFTseq
Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification of the causal alleles and their functions remain critical bottl...
https://www.protocols.io/view/craftseq-dm6gpz8ojlzp/v2
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www.protocols.io
BaglaenkoLab
9 months ago
I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.
www.nature.com/articles/s41...
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Precisely defining disease variant effects in CRISPR-edited single cells - Nature
A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...
https://www.nature.com/articles/s41586-025-09313-3#Sec8
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www.protocols.io
Anneke_P
10 months ago
The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at
@protocolsio.bsky.social
today. Can recommend; would publish again!! 🌾 🍞 🧪
dx.doi.org/10.17504/pro...
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Microplate Beta Glucan Assay
This assay measures the mixed linkage beta glucan content of cereal grain flour through specific, enzyme-catalysed, step-wise hydrolysis of the polysaccharide to glucose and qua...
https://dx.doi.org/10.17504/protocols.io.kqdg31e1el25/v1
1
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reposted by
www.protocols.io
Marta Niedzicka
11 months ago
Last month we published our protocol for detection of beet yellows virus. Here it is! RT-LAMP for BYV detection
www.protocols.io/view/rt-lamp...
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RT-LAMP for BYV detection
This protocol describes the process of detecting beet yellows virus (BYV) in plant material utilising a quick and easy colorimetric assay. The starting point is total RNA extrac...
https://www.protocols.io/view/rt-lamp-for-byv-detection-d6929h8e
0
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www.protocols.io
sylvain Brisse
11 months ago
qPCR diagnosis of
#diphtheria
: you may be interested by our procedure, which is now widely used across European reference laboratories:
www.protocols.io/view/identif...
@EURLdiphtheriaPertussis thanks
@chcrestani.bsky.social
for releasing on
protocols.io
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Identification of Corynebacteria of the diphtheriae Species Complex and detection of the diphtheria toxin g...
This protocol describes a quadruplex real-time PCR (Dip4plex), performed with the Qiagen QuantiNova™ Multiplex PCR Kit, for simultaneous identification of Corynebacteria of th...
https://www.protocols.io/view/identification-of-corynebacteria-of-the-diphtheria-8epv5o8jjg1b/v1
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3
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www.protocols.io
Emma Ganley
11 months ago
Happy to be in Brno for the
#CTLS2025
conference from the Core Technologies for Life Sciences society. I'll be talking about
protocols.io
tomorrow morning in the Core Facilities and Open Science session
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Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats."
@realdiehllab.bsky.social
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Protocol Spotlight with Maria Diehl
YouTube video by protocolsio
https://youtu.be/gFz4tfh5t98
11 months ago
0
0
1
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Stephen Heard
11 months ago
For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes!
scientistseessquirre...
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What your Methods section isn’t
If you’re a scientist like me, you read a lot of papers – and a lot of submitted manuscripts, drafts, and other larval stages of papers.* This is extremely helpful when it’s time to write your own …
https://scientistseessquirrel.wordpress.com/2025/06/03/what-your-methods-section-isnt/
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UCL Office for Open Science and Scholarship
11 months ago
A fantastic start to
#OSFest25
this morning, looking at publishing methods and protocols with
@protocolsio.bsky.social
. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place:
buff.ly/jZsSd6U
0
2
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www.protocols.io
Aligning Science Across Parkinson's
11 months ago
This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in
#ParkinsonsDisease
research 🐁 🧠 🔗 Check them out:
www.protocols.io/view/behavio...
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