Thrilled to share that our study on how the pcnB gene sustains Shigella virulence is now published in PLOS Pathogens: journals.plos.org/plospathogen... In short: pcnB ➝increased virulence-plasmid replication ➝ elevated T3SS expression ➝ enhanced invasion/spread within the intestinal epithelium.

Happy to announce that our paper is finally out! Thanks to @brozlab.bsky.social and co-authors @elliottbernard.bsky.social @ehartenian.bsky.social 🥳 the NLRP6 inflammasome is activated by sterile- or pathogen-induced endolysosomal damage @embojournal.org

IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇

Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei @sydneylmiles.bsky.social @katholt.bsky.social 🙏to transformative collaborative effort www.nature.com/articles/s41...

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Mast cell subsets respond uniquely to Salmonella infection. Read all about it in our latest paper in EJI. Another chapter in our deciphering of mast cell interactions with enterobacteria, driven by Chris von Beek. onlinelibrary.wiley.com/doi/10.1002/...

Such a rewarding collab it has been with our colleagues at #Novavax to dissect the MoA of their potent Matrix-M adjuvant. This project has really put the imaging skills of @erixoneriksson.bsky.social to the test. The fruits of this joint labour out now in NPJ Vaccines www.nature.com/articles/s41...

🦠 Launching a bimonthly digest on RNA modifications and related topics in bacteria!🤩 First issue: Summer 2025. Shared here + by email, future ones will be quicker reads! 😅 #rnasky #microsky #ribosome Subscribe and share if you are interested! rnamodifupdates.substack.com/p/rna-modifi...

#NatMicroPicks Decoding Shigella’s Virulence Blueprint 🦠🧬 Combining organoid infection models with genome-wide mutagenesis allows mapping the full set of Shigella genes required for human gut colonization www.nature.com/articles/s41... @sellinlab.bsky.social @natgenet.nature.com

Could lab-grown mini-organs help scientists research a range of infections? A new study published in Nature Genetics shows how researchers have mapped how the Shigella bacterium infects the human gut. Read our interview with study co-author — and UTM Biology prof — Lars Barquist at uoft.me/bI7.

Excited to be at #EESMicrobiology this week presenting our latest work on #Shigella gut #organoid infection screen from our recent Nature Genetics paper (see below)! Stop by poster 109 or just look for me, happy to chat and connect! #Microbiology #Shigella #Organoids #TraDIS

The pcnB gene sustains Shigella flexneri virulence https://www.biorxiv.org/content/10.1101/2025.06.16.659909v1

A study in Nature Genetics establishes the comprehensive genome-wide map of Shigella genes required to infect human intestinal epithelium. go.nature.com/4e7fdKp 🧬 🧪

New preprint out: www.biorxiv.org/content/10.1... We followed up on our recent Nature Genetics paper mapping Shigella colonization in intestinal organoids with a deep dive into one of the screen hits: pcnB. #Shigella #virulence #microsky

Thanks @sydneylmiles.bsky.social and @sergemostowylab.bsky.social for writing such a sharp and thoughtful editorial, which covers all the main points and potential caveats of our study published yesterday. www.nature.com/articles/s41...

Our latest work combines human gut epithelial #organoid culture with experimental #Shigella #infections, transposon mutagenesis, and statistical modeling. This has led to the mapping of the comprehensive geneset that drives Shigella epithelial colonization. Out @natgenet.nature.com: rdcu.be/eqGgf

Very excited about our latest combining advanced cell culture, transposon-insertion sequencing, and statistical modeling to map Shigella virulence determinants in organoid models. Great collaboration w/ @mldm.bsky.social @sellinlab.bsky.social in @natgenet.nature.com www.nature.com/articles/s41...

“we used mini organs, specifically mini-intestines, and infected them with a dangerous bacterium called Shigella” says @mldm.bsky.social‬ @sellinlab.bsky.social‬ (SciLifeLab & @uu.se) shows that cultured mini-organs can uncover how pathogens cause disease youtu.be/xG_66ZGd_fk

Thanks to lab-grown miniature intestines, researchers at #HIRI & Uppsala University have mapped how Shigella bacteria infect the human gut. This @natgenet.nature.com study opens the door to using cultured mini-organs to investigate a wide range of infections. 📍🦠 www.nature.com/articles/s41...

Thrilled to share our work is finally out at @natgenet.nature.com ! rdcu.be/eqGgf Combining a scalable human gut organoid model with TraDIS and statistical modeling, we mapped how Shigella colonizes the intestine at genome-wide resolution.

Out now @mbio.bsky.social !: We provide a time-resolved map comparing #Salmonella and #Shigella epithelial colonization strategies in human #enteroids and #colonoids. Connecting the dots among decades of molecular findings reg. underlying virulence factors. journals.asm.org/doi/10.1128/...

#EESInfection was very inspiring and enriching! Thanks for all new connections and for the opportunity to present our soon to be published story on TraDIShigella during infection of human intestinal organoids! Stay tuned to read the full story!

Only 328 days to go! We're very busy behind the scenes getting thigns ready for the meeting! In the meantime you can add your email address for updates here: coming-soon.embo.org/w26-04

Was great fun to have the emminent @lbarquist.bsky.social visit for the @scilifelab.se The Svedberg seminar this week. Few can explain computation and statistics so that even wetlab biologists get it, but here is one: www.utm.utoronto.ca/biology/peop... www.helmholtz-hiri.de/en/research/...

Today our favorite #mastcell infection-ologist Chris von Beek nailed his #PhD thesis to the group log. Looking forward to an exciting defense. They grow up so fast...

OUT NOW: Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice #microsky 🧪 www.nature.com/articles/s41...

Interested in predicting AMR in bacteria? We show ignoring phylogenetic structure in genome collections leads to overly optimistic evaluations of machine learning methods for AMR prediction. With @nwheeler443.bsky.social and former PhD student Yanying Yu. 🦠🧫🧬🖥️🧪 www.biorxiv.org/content/10.1...

New paper from the lab out now. ProQ prevents polyadenylation to stabilise mRNA in Salmonella. Fantastic work by Sofia Bergman and Christopher Birk.

Interested in collecting, sharing, and interacting with large collections of bacterial functional genomics data (RNA-seq, Tn-seq/TraDIS, etc.)? Check out our new web platform, Micromix, with case studies for Salmonella and Bacteroides! 🧫🧬🖥️🧪 Thread 1/n academic.oup.com/gigascience/...

My first post on bluesky couldn't be happier: The first preprint of my PhD is out 🥳 We show that the inflammasome receptor NLRP6 is activated by sterile or pathogen induced endolysosomal damage. Thanks to @elliottbernard.bsky.social and @brozlab.bsky.social, including Louise, Gaël and Vanessa

Our paper on 🦠queuosine 34 modification of tRNA tyrosine and antibiotic response is finally out in its final form in @eLife ... and quite different from the preprint version. Many thanks to reviewers, editors and kudos to all authors🙏 elifesciences.org/articles/96317

1/n Some time ago my colleague, excellent cook, and friend Ivan told me: "Cacio e pepe is the recipe that I screw up more often. Let's make a project studying systematically the physics of that sauce". Prepare to get cheesy, I'm glad to share the Cacio e paper preprint: arxiv.org/abs/2501.00536

Was a fantastic day to witness! Huge congratulations Dr. Petra Geiser!

Well, with the new activity here, thought I'd post some threads with some of our recent work -- starting with our paper where we use Bayesian statistical modeling to show bacterial transcripts have subminute half-lives, far shorter than previously thought 🧫🦠🧬💻 #RNAsky www.pnas.org/doi/10.1073/...

Latest out from our group: mast cells can combine PAMP sensing and effector-triggered immunity to differentiate between extracellular and invasive enterobacteria Read more here: rdcu.be/dxiSH