A #FIBSEM #cryoET drama in 4 acts. Prelude: Aquilos shuttle rod does not reach cryo stage due to mechanical misalignment.

Here we go again 🙃

Introducing Extended PDB IDs at RCSB PDB Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued. www.rcsb.org/news/fe...

Someone please stop the 3DEM auto-reply madness #cryoEM

First post here- Hello Bluesky! Quick science query: These 2D classes are from negative stain of a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep! #negativestainEM #pulldown #HEKcells

Results from CZII's #cryoET object detection challenge on kaggle are in! Participation exceeded our expectations with 931 Teams from 76 countries competing, generating over 27,000 submissions. For an overview check out our post-competition page: cryoetdataportal.czscience.com/competition

Check this out!!! The results from our CryoET particle picking competition are available, the full test data (public and private datasets) is available, and you can dive right into the data on the CZ CryoET Data Portal! Enjoy and please let us know what you think :)

I never felt such joy as I have until I temporarily upgraded to COOT 1, realized how terrible it is, and downgraded back to my old friend Coot 0.9. #CryoEM #TeamTomo

📢 Registration is now open for the @diamondlightsource.bsky.social Biological Cryo-imaging User Meeting 2025! 📍Join us in Nottingham on April 23, in-person & online. Plus, don’t miss the CCP-EM Spring Symposium (April 24-25)🦠#CryoEM #BCIUM2025 🔗Register here: www.diamond.ac.uk/Home/Events/...

The microscope comparison datasets (Glacios vs. Krios, EF vs. no EF) from our high-resolution/high-throughput #cryoEM SBDD workflow project (the one with more than a dozen ligand-bound 2 Å structures of an 85 kDa complex) have been released on EMPIAR: www.ebi.ac.uk/empiar/EMPIA...

Early Christmas present! @mathewmclaren.bsky.social @willstuart.bsky.social @daumlab.bsky.social

Was thinking about RELION5's new coordinate system and came to a realization... Was the switch to a centered coordinate system done to make sure that the coordinates of a particle are applicable to reconstructed tomograms of any size? #teamtomo

For the #ML and #CryoET folks out there: Are folks on here submitting to the CZII CryoET Object Identification competition: www.kaggle.com/competitions... ? The leaderboard is really interesting, and the forums are full of insights. How many of the latest picking models have been submitted?

We received our TFS Arctis this past weekend. This #cryoEM instrument is part of our initiative to expand the #cryoET biomedical research capabilities at the center. SEMC/NYSBC - Providing expertise and resources for understanding both molecular and cellular structures. Find out more at: nysbc.org

The underlying theory (in Fourier space) isn't too far away from that of crystallography. Hopefully the #relion tutorial and the #cryoEM video lectures at @mrclmb.bsky.social's YouTube channel will help too. 🤗

Cryosparc isn't the only tool you should RELION 🥲 #cryoEM

I'm going to run an intensive "boot camp" called "Practical Cryo-EM" where we learn how to freeze grids, align microscopes, collect images, and do 3D reconstruction and stuff. January 13-17

Greatly enjoying our ongoing #cryoET adventures. My new favourite way to procrastinate from administrative tasks is to play I spy with @bobcoelingh.bsky.social's recent data set! 👀 Here's an easy one! 👇

Anyone have any publicly available data (or data you want to share with me) that you'd love to see segmented? I find myself in need of some fun data to segment in my free time... #cryoET or #volumeEM!

What did you listen to most this year? #CryoEM Reposting this old meme on the new platform

Particle looks like a happy puppers eager to see me. #cryoEM

#TeamTomo What is the most user-friendly template matching software for cryo-ET with samples with rather low complexity? Can't do Warp now without Windows machine.

My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module. github.com/builab/subtomo…

FYI: Thanks to @cellarchlab.com's awesome open science practices, this data is available on the CZ cryoET data portal (dataset 10008) and @ebi.embl.org's EMPIAR (11058)! You can check out the tomograms in your browser here: cryoetdataportal.czscience.com/datasets/10008

Some #cryoEM people: The cup can only ever be 2/3 full!

CZII’s cryoET particle picking machine learning challenge is up and running. More than 3400 signups on Kaggle, great discussions going on, and leaderboard scores better than we were able to do! Start off at: cryoetdataportal.czscience.com/competition

I will slowly start (re)posting the #cryoEM #Science #Memes from my Tw/X profile. Let's start with this one

Let's get caught up, shall we? Over the next few weeks I'm going to share #sciart highlights from my 10 years as a professional medical illustrator. First up, my 2014 image of beta-galactosidase, created in collaboration with Doreen Matthies (NIH), illustrating #cryoem resolution breakthroughs

Vesicle getting engulfed by forming autophagosome inside axons. #cryoET #teamtomo

Reintro: I'm a neuroscientist interested in how cells use molecular topography to control signal transduction. I use #CryoET and mouse genetic tools to map the topography of synaptic proteins and determine in-situ structures of scaffolding interactions that control said topography.

Seems like its time for a re-introduction. I am a junior faculty at OHSU and a #teamtomo #cryoET enthusiast with a particular interest in membrane reorganization within mammalian cells. I develop the Surface Morphometrics toolkit (and some other bits and bobs).

A proper intro: I'm at Stanford currently and I study (3) things 🧪 1) #cryoEM and #cryoET #teamtomo of molecular machines: We put out a preprint on how the AAA+ machine Sec18 bypasses topology of all SNARE substrates by using 'side-release' and 'side-loading' (www.biorxiv.org/content/10.1...) 1/3

🌌 Seeing cryo-EM picking up on Bluesky, it's time for a reintroduction! 👋 I'm Ieva, a structural biologist (former crystallographer turned cryo-EM aficionado since 2014). I work at Thermo Fisher Scientific and am passionate about advancing #cryoEM & #cryoET applications in #DrugDiscovery 🔬💊

To the #CryoET folks: Is there any advantage to collecting tilt series in EER (vs. good old Tiff format.) for in situ CryoET? I saw a meeting report from July 2024 on the web related to this question, but could not find a publication (yet).

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

Starter packs are great for a maximally twitter-like experience, but I've been really into feeds, which let me sort by content rather than by specific people. Here's one for #teamtomo bsky.app/profile/did:...

😭

𝐄𝐥𝐞𝐜𝐭𝐫𝐨𝐧 𝐌𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 𝐒𝐩𝐞𝐜𝐢𝐚𝐥𝐢𝐬𝐭 post: deadline extended! Please, share it with whoever is seeking professional growth. "Junior" profiles strongly encouraged, you'll be put on the right training track by our Specialists. careers.humantechnopole.it/o/electron-m...

We got alpha helices ❄️🔬

If you don't have or want to use a personal or lab website and do cryo-ET I'll set you up with a @NAME.tomo.science handle.

Very excited that our pathogen cryo microscopy facility is starting to take shape... Can wait to plunge some infected cells and get to imaging! ❄️🔬🦠

"Is X-ray crystallography dead?" some asked when cryoEM got momentum. Depends on what you work on. For nucleosomes it certainly looks like it is dead: all structures with nucleosomes released in 2023 were cryoEM structures! @nucleosomepolice.bsky.social guillawme.github.io/insights-fro...

CryoEDS time with the material science colleagues :)

Oh dear….this might take a minute ❄️🔬

🤯😭😵‍💫

feels absurd to use almost 4000W with one keystroke. Should we return to server GPUs in the next build? #CryoEM

You always wanted a book on cryo- electron tomography, written by the leading experts in the field? I have good news-You can now pre- order this book, edited by Friedrich Förster and myself! link.springer.com/book/9783031...

#cryoEM peeps. ❄️🔬 What is your server build? Forward #ThinkMate schemes please!! for comparison

Successful screening access completed at the PNCC. The expertise of the team at the PNCC have provided me with so much knowledge and training that I am coming home a lot more confident in my abilities. I am very excited to apply this knowledge to the budding SPP-ARC facility!