We used our new ivRIC method to map host proteins inside HIV-1 particles, finding 100+ proteins that the virus hijacks to boost infection. This project has a long history, spanning from pre-COVID to now! Driven by Manuel Garcia-Moreno and led by @alf-castello.bsky.social tinyurl.com/54vb842w

Proud of Louisa Iselin for this work. Not only a covid baby but had both of her supervisors move lab (@alf-castello.bsky.social and me). The works is a meticulous analysis of the interferon pathway and specifially how RNA binding of proteins are modulated by PTMs. www.cell.com/cell-reports...

Proud of Louisa Iselin. Not the easiest PhD, covid, two supervisors (shabazlab.bsky.social) moving labs, but she pushed through and delivered a really solid piece of work on how interferon reshapes RNA-protein interactions post-translationally www.cell.com/cell-reports...

6/ This study is a collaborative effort with Arvind H. Patel & @alf-castello.bsky.social - @cvrinfo.bsky.social; Rafael Sanjuán - @i2sysbio.es & Miguel Chillón - @uab.cat 👇 Check the original article published in @natcomms.nature.com www.nature.com/articles/s41...

Delighted to collaborate with @melisupf.bsky.social on this exciting study. Juana Diez and her team reveal that coronaviruses reprogram the cellular tRNA pool to optimise viral gene expression 🤯🍾🥂🥳🥳

1/ 🎯 A therapeutic target that would curb the spread of #coronaviruses has been identified It is an enzyme that is activated in human cells in response to stress due to infection and causes changes in the cellular machinery that favour the production and spread of coronaviruses tuit.cat/5y9Rr

Thrilled to contribute to this awesome work led by Ben Davis and @shabazlab.bsky.social on in situ mapping of the in cellulo cysteinome, which opens lots of exciting applications www.nature.com/articles/s41...

Thrilled to contribute to @samjwilsonphd.bsky.social’s new Science paper showing that avian flu polymerase, via its PB1 subunit, stays active at fever temperatures, unlike human flu polymerase. A key factor in their different pathology. 🤯🍾🥂🎉

Thrilled to announce the successful thesis viva defense of Zaydah Roland de Laurent 🦟 on her work defining the RNA interactome of mosquitoes. So proud for her successful defence! Well deserved! #MosquitoResearch #RNA #Doctorate.

We’ll soon be advertising a postdoc position (3 years) to study how mRNA-specific translational regulation shapes the innate immune system, in collaboration with @alf-castello.bsky.social. The formal ad will be out soon but get in touch if you’d like an informal chat! #RNASky

Are you thinking on an exciting PHD project at the interface of translation control and innate immunity? @rnanomad.bsky.social and I have a project that might interest you!

Our new PLOS Pathogens paper with ‪@brennanlab.bsky.social‬ is out! We charted the first protein–RNA interaction map for virus-infected tick cells, revealing 283 RNA-binding proteins reshaped by Uukuniemi virus. Some directly control viral particle output. Full story: doi.org/10.1371/jour...

Post 5/5 🧵 IMPACT: This transforms tick-borne disease research: 🎯 New drug targets 🎯 Understanding vector competence 🎯 Virus-tick evolution Testing if mechanisms work across other tick viruses next. 👨‍🔬 collaborative project with @alf-castello.bsky.social and @alainkohlvirology.bsky.social

🚀 New from Cell: SPIDR is a game-changer—map dozens to hundreds of RNA-binding proteins simultaneously with single-nucleotide precision in one CLIP experiment. An exciting advance for RNA–protein interactomics! 🧬🔗https://doi.org/10.1016/j.cell.2025.06.042

I am really pleased we managed to squeeze this story out during #ASMS2025. This is our contribution relating to the fantastic Omnitrap/Orbitrap. What we thought would be a straightforward conversation of our exploris-omnitrap turned into quite a journey. (1/n) www.biorxiv.org/cgi/content/...

🚨🚨 News from the @castello-lab.bsky.social and @shabazlab.bsky.social. Our work, led by Louisa Iselin, reveals pervasive changes in the RNA-bound proteome induced by interferon. #RNA, #immunity, #interferon, #host-virus www.biorxiv.org/content/10.1...

Proud of Louisa Iselin for this work. Not only a covid baby but had both of her supervisors move lab (@alf-castello.bsky.social and me). The works is a meticulous analysis of the interferon pathway and specifially how RNA binding of proteins are modulated by PTMs. www.biorxiv.org/content/10.1...

Happy to see Honglin Chen's paper published! 🎉🎉🍾🥂. Great collaboration with @shabazlab.bsky.social to discover the discordances between RNA and protein in the interferon response...

A basic solution to a very common problem. collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social www.mcponline.org/article/S153...

Great conference at @embl.org on the expanding world of #RBPs. After the hard (fun) work, the @castello-lab.bsky.social chilled out in the beautiful Heidelberg

Pre-print is up! SARS-2 produces a truncated N protein to antagonize multiple antiviral pathways. A very collaborative project in the @corcoranlab.bsky.social with @nogasharlin.bsky.social, Danyel Evseev, and Max Bui-Marinos, and of course the boss Jenn Corcoran www.biorxiv.org/content/10.1...

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/... #RNAsky #RNAbiology #Cryo-EM

Excellent talk of Louisa Iselin on the remodelling of the RBPome by interferon at the "Expanding Universe of RBPs" in @embl.org, Heidelberg. The @castello-lab.bsky.social is very proud 🍾🥂🎊🎊

Translation of mRNA can be triggered with spatiotemporal control in zebrafish 🐟 embryos by light ⚡ & FlashCaps 🧪⚗😊 Kudos to all authors and collaborators 🙏👍 www.nature.com/articles/s42...

Amazing work of our dear friends @ilandavis.bsky.social and @jeffylee.bsky.social characterising the interlinked roles of two key RBPs in drosophila brain development, congratulations 🍾🥂🎉🎉🎉 www.science.org/doi/10.1126/...

Had a wonderful couple of days with these guys. @alf-castello.bsky.social @marvintanenbaum.bsky.social and Stephen carter. Always a pleasure

At this END of YEAR please check out our new paper on GENE ENDS and a convenient marker of transcription termination: RNAPII CTD T4ph! We think it is relevant to anyone studying human/animal #genes and #genome so put a lot of effort to make it easy to read. #RNABiology and #Epigenetics meet:)

These studies are so important. Well done to the authors and the journal for publishing it. This certainly applies to so many antibodies. Companies selling antibodies should do and provide the right controls before commercialisation. www.nature.com/articles/s41...

Please check out our paper in collaboration with Nahum Sonenberg's group. We show how some DNA viruses co-opt the host cell translation machinery to repress the cGAS/STING response. TLDR; the cap-binding protein 4EHP represses the cGAS mRNA translation. #RNA, #RNASky www.pnas.org/doi/10.1073/...

RECRUITING-PhD student to study the role of RNA-binding proteins in antiviral defence during early development. #crispr #iclip #omics Interested candidates, please email a full CV and a statement of interest. Open to both UK and overseas www.findaphd.com/phds/project...

Nuclear RBPs moonlighting in the cytoplasm as part of the host response to Sindbis virus. Another beautiful work by Alfredo Castelo’s group. I’m struggling to keep up with reading all the amazing papers they’ve published in the last few months. www.cell.com/molecular-ce...

I am just going to plug-in the latest paper from our lab, wherein we show that treatment with the chemotherapy agent 5-Fluorouracil (5-FU) induces ribosome collision and that RQC pathway mitigates the 5FU-induced cytotoxicity. #RNA, #RNASky, #Cancer academic.oup.com/nar/article/...

A deserved celebration after the hard work 🍾🥂. @waelskamel.bsky.social and collaborators from the @castello-lab.bsky.social discovered that the U2 snRNP displays a moonlighting antiviral role and uses the U2 snRNA to interact with viral RNA 🤯🤯🤯 t.co/DJlXFxmGEv

🎄Amazing @castello-lab.bsky.social Christmas lunch! Great food, fantastic company, and lots of fun. We even created some art masterpieces during our pottery painting session afterwards 🥳. #Lablife, #Christmasfun, #RNA, #virus

🦸 Hidden superheroes in our cells!? @waelskamel.bsky.social, @castello-lab.bsky.social & the Rosalind Franklin Institute have uncovered how a team of cellular “superheroes” leap into action to stop viruses in their tracks. More: gla.ac.uk/research/az/... The study: cell.com/molecular-ce...

Cool

Big news! We're looking for a new Professor (or Associate Professor) in Infection and/or Immunity! Come work with us in @cambridgebiosci.bsky.social, it's a collaborative and supportive environment with great resources, students and colleagues! www.jobs.cam.ac.uk/job/49381/ Deadline 26/1/25 (Pls RT)

Really important achievement.. Price to the most original lab costume for the @castello-lab.bsky.social in the @cvrinfo.bsky.social Christmas party. Inspired by the notes of the "Sound of Music"😁. 🍾🥂🏆

I am delighted that our long standing close collaboration with AlfredoCastello lab has led to yet another very exciting publication in Mol Cell: thanks so much to Wael and Alfredo for taking the lead. www.cell.com/molecular-ce...

I am now joining this platform. Nice to meet the #RNA and #virus community here