You want to build an apparatus for time-resolved cryo-EM? Easy: bio-protocol.org/en/bpdetail?...

Happy New Year! (and first post here 😀) Excited to share the final version of our work where we combine deep mutational scanning, cryo-EM, and molecular dynamics to further resolve the mechanisms of pH sensing in GPCRs. 🧵(1/n) www.cell.com/cell/fulltex...

Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1

Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover! We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product. biorxiv.org/content/10.1...

We will be hosting 3rd Full Circle Symposium for Native Biologists hosted at UCSF in April 2025! We aim to build community and provide meaningful opportunities for Native scientists. If you are a Native biologist who is interested plz fill this out to stay up to date: forms.gle/AN7nfkhdB3Fv...

Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1

as a Big Nerd I have to tell you I went to Mendel’s abbey to look at his peas but the most notable part is actually the little hats he made for his microscopes

Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇

A certain researcher has had 3 papers retracted due to obvious manipulation as in this example below. Saying that scientists cannot push for retraction because a paper is already peer reviewed or should shut up as they never tried to republish something completely wrong is not a good look

A first attempt to assemble GPCR aficionados here on 🦋 Let me know who I missed by replying below! go.bsky.app/75ukb81

I am already running a service to notify about new #SerialEM releases by email at nexperion.net/serialemupdatenotices, but considering to make this a Bluesky bot as well ... is this something the #teamtomo #cryoEM #cryoET community would be interested in?

I kind of forgot how to announce Feeds, but here goes For an all-in-one CryoEM Feed: #CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords. bsky.app/profile/did:...

"Time-resolved cryo-EM of G protein activation by a GPCR" #CryoEM #membrane #protein #structure #GPCR www.nature.com/articles/s41...

This one is for the #cryoEM afficionados. Ten years ago tomorrow (March 14th, 2014), I was at the Tahoe symposium on cryoEM 3D image analysis (taking place again this year, starting today!) and attended a talk by Alberto Bartesaghi, then from NCI-NIH now Duke.

Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1

Super cool study on the nicotinic receptor using time-resolved cryoEM! www.cell.com/cell/fulltex...

Some rings for #FluorescenceFriday: spectrin labeling of the axonal periodic scaffold seen by 3D-STORM. Did you know that rings are synchronized between axons when bundle together? Now you do!

Here's an exciting job for the right person. Chance to join the senior staff at EMBO. Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work. www.embl.org/jobs/positio...

Enlightening interview with David Gloriam about how to properly measure GPCR ligand bias. youtube.com/watch?v=g5PX...

www.sciencedirect.com/science/arti... Our Cell paper appeared today: "Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" -- New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.

Science Writers: NASW is transitioning from Twitter to Bluesky as its short-form social media platform for community building. To facilitate Bluesky signups for our member network, we've created a code donation and receiving tool. See you all here soon! www.nasw.org/article/nasw... 💚

brunch with your boss

Molecular determinants of ligand efficacy and potency in GPCR signaling www.science.org/doi/10.1126/...

Not sure how many SerialEM users in #cryoEM and #cryoET are already here, but: SerialEM 4.1 was just released. It comes with many useful new features and script commands, thanks to the tireless work of David Mastronarde bio3d.colorado.edu/ftp/SerialEM...

This is great work and is written very clearly. Making cryoEM more accessible/affordable ❤️❤️❤️

Stanford is looking for a new CryoEM Facilities Manager #cryoEM #cryoET #structure #microscopy careersearch.stanford.edu/jobs/cryo-el...

Selective activation of Golgi-localized β1AR www.biorxiv.org/content/10.1...

A beautiful selection of near-infrared fluorescent dyes that our M.Chem. project student, Iain, is investigating! 🤩 #chemistry

"We asked students several questions about the scheduling structure, frequency, and helpfulness of their individual meetings with their advisors. Frequent meetings were a positive predictor of nearly every aspect of well-being and productivity measured in the survey."

🥳

I will be giving a virtual talk about our latest work on baited 🎣 reconstruction for in situ structure determination next Wednesday the 1st of November. Use the following link to find the abstract and info on how to tune in! #cryoem

Cyclic peptide inhibitors stabilize Gq/11 heterotrimers https://www.biorxiv.org/content/10.1101/2023.10.24.563737v1

I am delighted to announce I will be joining Baylor College of Medicine Biochemistry & Molecular Pharmacology as an Assistant Professor in January 2024! Huge thanks to CPRIT and NICHD for generous additional startup funding; I’ll be hiring at all levels soon!

Now on @biorxivpreprint.bsky.social: Dari Kimanius' paper on #Blush regularisation, a machine-learning based prior in #RELION5 trained on EMDB #cryoEM half-maps, which he uses to solve a #cryoEM data set of a 40 kDa protein:RNA complex that was previously intractable! www.biorxiv.org/content/10.1...

New preprint on EMMIVox, an approach and software for accurate model and ensemble refinement using cryoEM maps and Bayesian inference 🧶 🧬 🧪 ❄️ 🔬 Work led by Samuel Hoff from Max Bonomi’s lab and with contributions also from Emil Thomasen doi.org/10.1101/2023...

DynaMight: estimating molecular motions with improved reconstruction from cryo-EM images https://www.biorxiv.org/content/10.1101/2023.10.18.562877v1

Membrane Mimetic-Dependence of GPCR Energy Landscapes https://www.biorxiv.org/content/10.1101/2023.10.16.562552v1

Next up in our preparation for #RELION5 will be a @biorxivpreprint.bsky.social to describe #DynaMight: our solution for ’exploring protein *Dyna*mics that *Might* improve your map’ by the amazing Johannes Schwab. #staytuned #cryoEM

come work with us in beautiful Boulder. Job opening in the CU Boulder Center for CryoET. Do you like tomography? and bears in your yard? and marmots in the mountains? check it out jobs.colorado.edu/jobs/JobDeta...

I'll be looking to hire another research associate (>$100k/year + benefits & retirement) shortly. Experience in molecular biology and pharmacology would be ideal, but not essential. Job ad will go up in a bit, but if anyone knows anyone looking I would be happy to chat with them.

Hungry for science? 😋 Have a feed! 🍴 Here's a searchable list of over 50 science-related feeds on BlueSky. Find the stuff you're interested in and tag your own posts to get them included. #AcademicSky 🧪

Fantastic work by Merritt Maduke's group, using CryoEM to resolve the structure of my good old friend from grad school, the chloride channel ClC-2. Confirming the ball-and-chain gating model we postulated based on deletion/chimeric studies 1992/97. www.biorxiv.org/content/10.1...

So proud to share our first paper examining the role of #xylazine in the drug supply! This work is the definition of a team effort! www.biorxiv.org/content/10.1...

My thread from yesterday may not sufficiently convey the variability PIs experience when contacting POs. Yes, it runs a gamut from being ignored to…funding your middlin’ score grant. When you get ghosted, well, sometimes POs change ICs, retire or die. Plenty of reason to contact another one.