What is a promoter? And how does it work? We very happy to share our latest work trying to understand enhancer-promoter compatibility. I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters www.biorxiv.org/content/10.1...

How does a “universal” transcription factor evolve to do species-specific jobs? Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes. Read it here 👉 www.biorxiv.org/content/10.1... #MolecularEvolution #Transcription

Flung some discs, shared some laughs, and capped it off with good food. Summer vibes from the Teves lab!

Vancouver showing pride

So excited to see this work out in @narjournal.bsky.social academic.oup.com/nar/article/... Congratulations to Hazel Cui on outstanding work! Keep an eye out for part 2 of this project 😎

In #GENETICS, @sheilateves.bsky.social et al. study the regulation of Pol III transcription during different stages of heat shock stress in mouse embryonic stem cells, showing that the dynamic regulation & recovery of Pol III may be exclusive to the heat shock response. Read more: buff.ly/s966iZ1

So proud of Thomas Nguyen and co-authors on the publication of this manuscript. Now out in Genetics! See full paper here! academic.oup.com/genetics/adv... Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback

Really fun chatting with JP, Enya, and Lawrence!

The Teves lab totally glammed it up at the departmental holiday party!

Finally out! We present EXTRA-seq, a new EXTended Reporter Assay to quantify endogenous enhancer-promoter communication at kb scale! www.biorxiv.org/content/10.1... A 🧵about what it can do: #SynBio #DeepLearning #GeneRegulation

Sunshine and rainbows for the little dude

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social! tinyurl.com/setd2

We just posted a preprint about a death mechanism that we find truly surprising. When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?! preprint here and a thread (1/n): www.biorxiv.org/content/10.1... 🧪

🎄🔔Ho Ho ho!😆 Our final #FragileNucleosome seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists! 🗓️Don't forget to register: us06web.zoom.us/webinar/regi...

"Women full professors are 19% more likely than men at the same career stage to leave academia, as compared with 6% for women assistant professors and 10% for associates."

1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇 www.biorxiv.org/content/10.1...

Thrilled to share a new study from the lab revealing an H3K18ub-H3K9me3 crosstalk mechanism, roles for UHRF1 and its E3 ligase activity in regulating a hierarchy of repressive histone methylation signaling, and a strategy for targeting SUV39H1/H2 to improve DNMT1 inhibitor efficacy! #epigenetics

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs. You can check out our pre-print here: tinyurl.com/27p7hyt9, and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

All editors rely on Pol3 because we use its promoters to transcribe the gRNA from plasmid vectors. I never thought something so cell-essential would be regulated. -> food for thought wrt editing cell types in various states.

🎉Excited to share our new preprint on Biorxiv 🥳 We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress! So what did we find? 🧵 www.biorxiv.org/content/10.1...

Very interesting paper from @mitchguttman.bsky.social previously in @biorxivpreprint.bsky.social out in Nature Genetics. "ChIP-DIP generates context-specific protein localization maps at consortium scale" www.nature.com/articles/s41...

Machine-guided design of cell-type-targeting cis-regulatory elements www.nature.com/articles/s41... @natureportfolio.bsky.social

Beautiful Squamish mountains on the disc golf course

The strain on scientific publishing: we set out to characterise the remarkable growth of the scientific literature in the last few years, in spite of declining growth in total scientists. What is going on? direct.mit.edu/qss/article/... A 🧵 1/n #AcademicSky #PhDchat #ScientificPublishing #SciPub

(1/n) What better way to start posting on Bluesky than to share our recent preprint, in collaboration with @timhughesto.bsky.social, on characterization of the intrinsic DNA binding specificities of 179 human transcription factors using a new assay, GHT-SELEX: www.biorxiv.org/content/10.1...

Hey new and seasoned BlueSkeeters! For our next #FragileNucleosome seminar session, we are happy to host another three amazing ECR scientists: Sanne, Vivek and Thomas! 🗓️Don't forget to register: us06web.zoom.us/webinar/regi...

If this exists already.. I feel you can never have too many pet packs. Please let me know if you'de like to join. go.bsky.app/4TA4G8D

First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5

Now that half of SciTwitter is here… Come be my colleague! The Dept of Cell Biology @EmoryUniversity is recruiting a developmental cell biologist. 🔬Do you study mechanisms of development using cell biological (and other) approaches? Join us!🔬 TY for RT! jobs.sciencecareers.org/job/664684/t...

🔬 Are you eager to dive into the world of light microscopy & learn from top-notch microscopists about its essential techniques for life science applications? If that sounds exciting, don’t miss out on this free, in-depth iBiology series! 👉 www.ibiology.org/online-biolo...

Reintroducing myself to all the new folks here

The latest from our group, led by Megan Ostrowski and @martyyang.bsky.social, is now published in final form (www.cell.com/cell/fulltex...! Many thanks to our excellent peer reviewers for suggesting several experiments (including CAF-1 perturbation) to really improve the study =) #epigenetics

REX - a mammalian "range extender" element that can turn short-distance enhancers into long-distance enhancers. New preprint from a collaboration led by Grace Bower and Evgeny Kvon. doi.org/10.1101/2024...

Looking for a tag to localise fluorescent proteins to the plasma membrane? Unsure which tag might work in your species? We generated a toolkit of 11 membrane-localising tags, which can be screened rapidly by microinjecting mRNA in your species of interest. 1/4 www.biorxiv.org/content/10.1...

One of the most ancient sequences in biology, the peptidyl transferase center of the ribosome, is not as conserved as we might have thought. #RNA #Ribosomes www.biorxiv.org/content/10.1...

Putting together packs of transcription and Chromatin people - Nominate others to add! go.bsky.app/5zgpZfg

How do distal enhancers control transcription dynamics in living cells? Check out our work published in @MolecularCell!! We show that the dynamic assembly and disassembly of TF clusters at enhancers are a major source of transcriptional bursting. www.cell.com/molecular-ce...

Check out our new review paper published in Trends in Cell Biology ! We discuss new models of enhancer functions, including emerging 'transcription hub' model in gene regulation. "Regulatory Landscape of Enhancer-mediated Transcriptional Activation." www.cell.com/trends/cell-...

Hello new followers! Will start posting here more often. Reposting our recent preprint on intercellular tau transmission! 🧪🧠 #neuroscience #cellbiology

Fall on the disc golf course

Latest research from the Teves lab is now out on Biorxiv! "Functional divergence of TBP homologs through distinct DNA binding dynamics" biorxiv.org/content/10.1... Here, we examine how conservation is balanced with diversification throughout evolution, using TBP as a case study.

Happy Halloween from the Teves lab pH strip!

Back to school for the Teves lab

New preprint on Sox2 dynamics showing a temporal segregation between TF-enhancer binding and Pol2 gene transcription... very cool stuff from Francois lab! www.biorxiv.org/content/10.1...

🚨 New Teves Lab Publication Alert! 🚨 Excited to share our latest research published in @RNABiology: "TBP facilitates RNA Polymerase I transcription following mitosis". Read the full article here: tandfonline.com/doi/full/10....

Is there a study showing that human SOX2 can replace mouse Sox2 in mESCs? If so, please share a paper :D TIA!

Huge congratulations to the newly minted Dr. James Kwan and Dr. Thomas Nguyen! These 2 students joined a brand new lab in 2018, went through the pandemic, and emerged as full-fledged scientists. The first graduates of the Teves lab... a MAJOR milestone!

A foggy sunrise makes for beautiful pictures

Thank you to CIHR for another 5 years of funding to the Teves lab!! Fantastic news! And all because of the talented trainees in the lab making all the great science happen. Onwards and upwards!