Ioannis Tsirkas
@ioannistsirkas.bsky.social
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📥 313
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Post doc and manga lover, I will be sharing science-related stuff and manga reads.
reposted by
Ioannis Tsirkas
bioRxivpreprint
17 days ago
Real-Time Segmentation and Classification of Birdsong Syllables for Learning Experiments
https://www.biorxiv.org/content/10.64898/2025.12.19.695629v1
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Mech Arena
link.plrm.zone/sdlgo/ki0l4
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Get Mech Arena
Download the game and send me a Friend invite!
https://link.plrm.zone/sdlgo/ki0l4
25 days ago
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It was great to be part of this collaboration, I always admired Prof Maya Schuldiner's and Dr Ofir Klein's work.
lnkd.in/dfabHSkx
For the original papers developing the fluorescent scFvs technology in yeast:
lnkd.in/d_KZ8WMK
lnkd.in/dxt9MD6d
about 2 months ago
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Ioannis Tsirkas
Wiep Klaas Smits (ExpBac/CMAT)
5 months ago
DNA replication initiation timing is important for maintaining genome integrity | Journal of Bacteriology
journals.asm.org/doi/10.1128/...
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DNA replication initiation timing is important for maintaining genome integrity | Journal of Bacteriology
The regulation of DNA replication is fundamental to cell growth and cell cycle control. In eukaryotes, under-initiation or over-initiation leads to genome instability. In bacteria, it is unclear how c...
https://journals.asm.org/doi/10.1128/jb.00175-25
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Ioannis Tsirkas
Hamperl Lab
5 months ago
Such a nice review for one of our latest research works!
add a skeleton here at some point
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We look forward to the community's feedback!
add a skeleton here at some point
5 months ago
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Ioannis Tsirkas
Mother Jones
5 months ago
The BLS did exactly what it's supposed to do: provide accurate, nonpartisan data. When the numbers showed Trump's "economic boom" was actually a bust (33k jobs vs claimed 291k), he fired the messenger. After all, when it rains too much, true leaders fire the meteorologists.
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We’re officially in Donald Trump’s mad king era
All the books that are fit to cook.
https://www.motherjones.com/politics/2025/08/bureau-of-labor-statistics-firing-were-officially-in-donald-trumps-mad-king-era/
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Ioannis Tsirkas
MadScientist
6 months ago
I don't like the person I become when designing primers
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Ioannis Tsirkas
Jop Kind
6 months ago
I am very excited to share our latest work where we describe a new method to profile genome-wide chromatin transitions over time in single cells. Great collaborative effort with the van Oudenaarden group
@hubrechtinstitute.bsky.social
@oncodeinstitute.bsky.social
www.nature.com/articles/s41...
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Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation - Nature Cell Biology
Kefalopoulou, Rullens et al. develop Dam&ChIC to assay chromatin state at two different time points in the same cell. The method was used to study the reorganization of LADs during cell division a...
https://www.nature.com/articles/s41556-025-01687-w
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Ioannis Tsirkas
bioRxiv Cell Biology
6 months ago
RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3
https://www.biorxiv.org/content/10.1101/2025.07.14.664777v1
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Ioannis Tsirkas
ME Torres-Padilla
6 months ago
🎤 Apply to speak @our TALENT FORUM by July 31! Showcase YOUR research in Epigenetics & NucleicAcids
@www.helmholtz-munich.de
& connect with leading scientists 🧳Travel+accommodation covered if selected! 📝 Info+how to apply:
lnkd.in/drkRvz7N
#postdoc
#career
#groupleader
#HelmholtzMunich
#research
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Great week for
#manga
readers!
#WildStrawberry
ch45
#Yattara
ch18
#DramaQueen
ch23
#Centuria
ch56
#Dandadan
ch198
#Chainsawman
ch206
#Bugego
ch10
#MärchenCrown
ch11
#TheGodofTime
ch5
#AsurasVerdict
ch14 Especially the last one was so good! Here are a few artworks.
7 months ago
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Ioannis Tsirkas
bioRxiv Cell Biology
7 months ago
A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms
https://www.biorxiv.org/content/10.1101/2025.06.02.657494v1
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#spoileralert
#WildStrawberrt
#Manga
with itdld best double spread. Too sad this is getting axed ;(
7 months ago
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Ioannis Tsirkas
7 months ago
Excited our paper is out! G-quadruplex (G4) unwinding via an intricate G-loop assembly and disassembly mechanism maintains genome integrity. Pioneered by Koichi Sato with great collaborators Jing Lyu and
@simonelsasser.bsky.social
Check it out:
www.science.org/doi/10.1126/science.adr0493
🧵(1/4)
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RNA transcripts regulate G-quadruplex landscapes through G-loop formation
G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics are controlled remains poorly understood. Here, we report that RNA tra...
https://www.science.org/doi/10.1126/science.adr0493
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#spoileralert
#dandadan
#manga
nailed it this time, one of its coolest
#artworks
! Remember, this is a weekly
#manga
!
7 months ago
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www.nature.com/articles/s41...
POPtag is officially my new favourite named tag! BTW, nice work!
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The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems - Nature Communications
“Intracellular phase separation is emerging as a universal principle for organizing biochemical reactions in time and space. Here the authors show that PopZ condensate dynamics support cell division a...
https://www.nature.com/articles/s41467-022-33221-z
7 months ago
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This was a gift from a colleague, and it looks breathtaking. Done within one day (1000 pieces, around 12-15 hours)
7 months ago
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Ioannis Tsirkas
8 months ago
A rather remarkable retraction for a paper where apparently no authors contributed! I guess these
#papermill
authors were a bit too honest...
#fraud
@elsevierconnect.bsky.social
doi.org/10.1016/j.ij...
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Ioannis Tsirkas
Andrew Deans
8 months ago
CST complex blocks end resection by BLM-DNA2 or EXO1, therefore promoting non-homologous endjoining and inhibiting repair templated DNA repair.
www.science.org/doi/10.1126/...
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CTC1-STN1-TEN1 controls DNA break repair pathway choice via DNA end resection blockade
Antagonistic activities of the 53BP1 axis and the tumor suppressor BRCA1-BARD1 determine whether DNA double-strand breaks (DSBs) are repaired by end joining or homologous recombination. We show that t...
https://www.science.org/doi/10.1126/science.adt3034
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Ioannis Tsirkas
Andreas Panagopoulos
8 months ago
Delighted to share that the results of my main postdoc project are now published in
@nature.com
🔗
doi.org/10.1038/s415...
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Multigenerational cell tracking of DNA replication and heritable DNA damage - Nature
A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early even...
https://doi.org/10.1038/s41586-025-08986-0
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Ioannis Tsirkas
Helmholtz Munich
8 months ago
Grab a pint & dive into science! 🍻 Pint of Science is back in Munich: 🗓️ May 19–21, 2025 🕢 19:30–22:00
#HelmholtzMunich
researchers share big ideas – from AI to antibodies. 🎟️ Info & tickets:
t1p.de/lvpuv
#pint25
#pint25de
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Pint of Science Munich
Explore Pint of Science Deutschland events in Munich
https://pintofscience.de/events/munich
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Spoiler alert!
#Dandadan
#manga
#194 does not disappoint this Monday too! Back2back peak chapters!
8 months ago
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Ioannis Tsirkas
Alessandra Brambati
8 months ago
Excited to share our latest from my postdoc at
@mskcancercenter.bsky.social
in the Sfeir Lab, in collaboration with the Simon Powell Lab! We uncovered a novel genome repair mechanism in human cells: RNA-templated DNA double-strand break repair (RT-DSBR). (1/5)
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Ioannis Tsirkas
Kat Abughazaleh
8 months ago
I used to have nightmares about getting a pet and forgetting to feed it. I have not had a single one of those dreams since getting Heater because there is not a chance in Hell she will let me forget breakfast.
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Ioannis Tsirkas
Crystal Rogers, PhD
8 months ago
I just woke up from a nightmare where I was giving a guest lecture to an undergrad cell biology class and none of them knew the central dogma or what DNA or RNA were. I spent an hour teaching that instead. They didn’t understand why they needed to know about DNA for cell biology. 🫠😫😱
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Ioannis Tsirkas
Rebecca Ann Jones
8 months ago
What governs where DNA replication begins in human cells? Here, we show that origins of replication in human DNA are epigenetically specified and that crucially, this epigenetic mark is required for both DNA replication and cell proliferation!
@narjournal.bsky.social
academic.oup.com/nar/article/...
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reposted by
Ioannis Tsirkas
bioRxiv Cell Biology
8 months ago
Fluorescence recovery in the Super-Resolution regime reveals subcompartments of 53BP1 foci
https://www.biorxiv.org/content/10.1101/2025.05.07.652606v1
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Ioannis Tsirkas
eLife
8 months ago
Ever wondered how cancer cells “decide” to spread? When high-grade breast cancer cells get crowded, they shrink and become more mobile, turning a non-invasive tumour into a threat. Scientists have identified the molecular switch behind the change.
buff.ly/8JIDzDF
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www.biorxiv.org/content/10.1...
Cool work!
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PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single experimental workflow.
Chromatin Immunoprecipitation (ChIP) and Co-Immunoprecipitation (CoIP) assays are the most common approaches to characterize the genomic localization and protein interactors, respectively, for a prote...
https://www.biorxiv.org/content/10.1101/2025.04.27.650851v1
8 months ago
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Ioannis Tsirkas
Daniel Jost - Physical Biology of Chromatin group
8 months ago
🚨 new preprint from the lab. Combining modeling, new Hi-C data in yeast and data analysis, our study offers new insights into the spatial and dynamic organization of chromatin during replication in eukaryotes. Check the tweetorial below ⬇️
www.biorxiv.org/content/10.1...
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Genome-wide modeling of DNA replication in space and time confirms the emergence of replication specific patterns in vivo in eukaryotes
Although significant progress has been made on our understanding of DNA replication and spatial chromosome organization in eukaryotes, how they both interplay remains elusive. In particular, from the ...
https://www.biorxiv.org/content/10.1101/2025.04.23.650322v1
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#StellarFriends
#manga
latest issue was really peak!
9 months ago
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Ioannis Tsirkas
Tony Southall
9 months ago
New preprint! We have discovered that the splicing factor Sex lethal (Sxl - well known for its role in sex determination) binds to chromatin and regulates tRNA synthesis in neurons by interacting with RNA Pol III! Highlighting Sxl as a novel modulator of neuronal homeostasis.
tinyurl.com/s44s2z95
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Sex-lethal is recruited to chromatin to promote neuronal tRNA synthesis in males through RNA Polymerase III regulation
The RNA-binding protein Sex-lethal (Sxl) is classically known as a master regulator of sex determination and mRNA splicing in Drosophila melanogaster. However, this role is not conserved across specie...
https://tinyurl.com/s44s2z95
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Another one is done, this was very nice, but tough.
#jigsaw
9 months ago
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Ioannis Tsirkas
Cody
9 months ago
“But you know what the oddest thing was? No one seemed shocked or surprised. Not even me. Yes, I was scared, but really, I just had the most profound sense of déjà vu.” X-Men ‘97 - 1x08
add a skeleton here at some point
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New
#manga
, The War of the Adults. Very interesting orwelian concept
9 months ago
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Ioannis Tsirkas
Prasad Jallepalli, MD, PhD
9 months ago
RFK HHS
add a skeleton here at some point
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Ioannis Tsirkas
Joachim Goedhart
9 months ago
Alpha-BET: Functional labeling of envelope glycoproteins with single domain antibodies for in-virus single molecule imaging by Sergio Padilla-Parra and team:
www.biorxiv.org/content/10.1...
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Ioannis Tsirkas
Michael Hendricks 🇨🇦
10 months ago
There are very fancy microscopes, but most are overpriced commodities in an un-competitive industry that sells you 100 features you don't want for every one you do. And, unless you're a cell biologist or mouse-ologist, no one makes a microscope for the experiment you want to do.
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Ioannis Tsirkas
Lucas Farnung
9 months ago
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by
@felixsteinruecke.bsky.social
with support from
@jonmarkert.bsky.social
!
tinyurl.com/replisome
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reposted by
Ioannis Tsirkas
bioRxivpreprint
9 months ago
Unexpected Molecular Mechanism of Orc6-Based Meier-Gorlin Syndrome: Insights from a Humanized Drosophila Model
https://www.biorxiv.org/content/10.1101/2025.03.31.646466v1
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Ioannis Tsirkas
Mark Hedglin
9 months ago
Excited to share our new pre-print. Direct, ensemble FRET assays developed by our lab reveal that assembly of Rad51 filaments at stalled replication sites via RPA/Rad51 exchange causes complete & irreversible PCNA unloading. Big implications for interplay between human DNA damage tolerance pathways
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https://www.biorxiv.org/content/10.1101/2025.03.27.645792v1
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#Chainsawman
#manga
SPOILER ALERT! No way! Fami?
9 months ago
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Spoiler alert!
#Chainsawman
#manga
peak for one more week!
10 months ago
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Ioannis Tsirkas
c0nc0rdance
10 months ago
When you've been in the cell culture hood all day and finally get to take off your gloves:
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#versus
#manga
gets only better and better!
10 months ago
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Ioannis Tsirkas
Vindigni Lab
10 months ago
Check out our new paper led by
@seppai99.bsky.social
from the Vindigni Lab in collaboration with
@cejkalab.bsky.social
delving into the role of nucleases resecting ssDNA gaps generated by PARPi. You can read more here:
genesdev.cshlp.org/content/earl...
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Ioannis Tsirkas
Sarah Aitken
10 months ago
My lab at hiring! - we have TWO postdoc positions (one computational, one wet lab focussed) at Yale
@yaleschoolofmed.bsky.social
, working closely with our group members at University of Cambridge, UK
@cambridgeuni.bsky.social
Please help to spread the word! Adverts with full details below ⬇️
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#AsurasVerdict
#manga
always gives me the chill, I love it! Because, you cannot spell Hello without Hell.
10 months ago
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#Dandadan
#manga
cooked again, with Lord of the Flies!
10 months ago
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