Latest imaging data from our completely re-designed ExA-SPIM system. Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.

Thank you @paveltomancak.bsky.social @olafselchow.bsky.social @dvonwangenheim.bsky.social @bundschuhst.bsky.social @ereynaud.bsky.social @cuencam15.bsky.social Vlado, Stephan for a life-changing lightsheet experience towards even more beautiful and high-quality science

... and @dvonwangenheim.bsky.social's great video montage of a few of the samples. #LISH25 🔬🌍: www.intelligent-imaging.com/AxL

Huge thanks to @paveltomancak.bsky.social and @embo.org for hosting this year's amazing Light Sheet Microscopy course #LISH25. Our @dvonwangenheim.bsky.social and team were thrilled to capture beautiful images of many samples with AxL Cleared Tissue LightSheet 🔬 🌍 www.intelligent-imaging.com/AxL

#LISH25 final student team presentations. #Specimen_Illuminati

#LISH25 final student team presentations. #SPIMply_the_Best

#LISH25 final student team presentations. #Liv_and_Dye

#LISH25 final student team presentations. #The_Holy_de_Broglies

An AD mouse brain with therapeutic antibody (in blue) and smooth muscle cells (in yellow).The first attempt, but quite successful. Thanks to @dvonwangenheim.bsky.social #LISH25

The world behind LS7 and Blaze starts with 3i for me. Very excited to see the result of an overnight imaging #LISH5 #AD #neuroscience

Super excited to show you our solutions for vertical plant microscopy at 3i @the.3i.social 🌱🔬 Or just swing by and say hello to Ben @bta3i.bsky.social and Daniel @dvonwangenheim.bsky.social 🙂 #ICAR2025

🔬data courtesy of @trevormnolan.bsky.social @xiaohuili.bsky.social - thank you!

Cell Division. Elongation. Differentiation. Capture the full spatiotemporal dynamics of root development with PlantScope automated montage-tracking! See 3i's @dvonwangenheim.bsky.social present at #ICAR2025 Friday June 19 at 9AM 🌍: www.intelligent-imaging.com/plantscope

🔬data courtesy of @trevormnolan.bsky.social @xiaohuili.bsky.social - thank you!

🌱 Excited to share that I will start my lab at EPFL, CH this June supported by a @snsf.ch Starting Grant! ramakrishna.ch We are interested in plant mineral nutrition and will deep-dive into the fine-tuned mechanisms employed by plants to adapt to abiotic stresses such as salinity. #newPI #plantsci

🌱 When a root cell undergoes symmetric division, are the daughter cells actually identical? By combining live-cell imaging and scRNAseq we discovered a new cell state with uneven BR activity. Read our full paper in Cell: doi.org/10.1016/j.ce... @nvukas.bsky.social @trevormnolan.bsky.social

🌱 Thrilled to see our latest collaborative work with @jennyrussinova.bsky.social @nvukas.bsky.social published in Cell! By combining single-cell RNA-seq and live imaging, we uncovered previously hidden brassinosteroid signaling states and their role in optimizing root growth. doi.org/10.1016/j.ce...

It's #3DThursday!🔬🌈 We had a great week at the Harvard Tissue Clearing Workshop, huge thanks to: @edwardlachica3.bsky.social @the.3i.social @harvardmed.bsky.social @bostonchildrens.bsky.social Imaged: Dan Giardina & Ed Lachica, 3i Visualized: syGlass.io Sample Prep: Dharmendra Puri, HMS, BCH

Tried a new fixation method for 3D imaging of clear tissues using iDISCO. What a difference! 4% para on the left. New method on the right. Stained MBP in magenta and Veins in green. Images captured using AXL from 3i. @edwardlachica3.bsky.social @dvonwangenheim.bsky.social @samuelconnell.3i.social

@the.3i.social is ready to roll with its #lightsheet #microscope at @paulamonterollopis.bsky.social Tissue Clearing workshop #TCW2025 See you Monday!

Oh, just playing around with the #AllenBrainAtlas in #SlideBook for #FluorescenceFriday... 🔬 www.intelligent-imaging.com/AxL 🧠 brain-map.org 🖥️ www.intelligent-imaging.com/SlideBook

Give SlideBook Scripting Superpowers! 🦸‍♂️🖥️🔬🧠 Announcing two new modules for SlideBook #microscopy #software: Union and Synergy. Implement analysis and imaging pipelines best suited for your science including machine learning and deep learning 🌎: go.intelligent-imaging.com/SlideBook-Un...

@edwardlachica3.bsky.social @dvonwangenheim.bsky.social @samuelconnell.3i.social @lsfm-na.bsky.social Mouse CNS immunostaned for ACTA2 and Myelin Basic proetein in Green. Capured with 3i lightsheet in static mode.

Imaging and stitching the entire cleared mouse CNS stained with an antibody that detects Smooth muscle cells in the arterial system, using a 3i lightsheet microscope. @edwardlachica3.bsky.social @dvonwangenheim.bsky.social

I am lucky! - Spectacular cardioid ♥️ sample from Lavinia Ceci Ginistrelli at IMBA, Vienna @imbavienna.bsky.social - Isotropic resolution using AxL Cleared Tissue LightSheet 🔬@the.3i.social - Breathtaking visualization in syGlass VR 😎 @michael-syglass.bsky.social

MagLOV! MagLOV is a fluorescent protein you can "turn off" with a handheld magnet, engineered by @mariaingaramo.bsky.social. We think/hope we can use it to turn arbitrary proteins (including drugs!) on and off in opaque creatures (including humans!). bsky.app/profile/addg...

I have written a couple of threads 🧵 on "classic" #PlantScience papers. I always tag them with #PlantScienceClassics , but will also compile them here in this thread: 👇

@edwardlachica3.bsky.social Mouse Spinal cord Sulcal arteries captured using ASLM-3i Light sheet microscope

Here's a word cloud of the academic titles I've co-authored. Generated using scholargoggler.com by @morinryan.bsky.social

Our booklet on Optical Tissue Clearing can be downloaded for free and without registration here: health.uconn.edu/cell-analysi...

Happy to connect here on bsky -- great to be here for #science, #neuroscience, #imaging, and #VR. Please add me! I've written a short blog on the silent 3D imaging revolution happening now in science. Check it out and let me know what you think! www.syglass.io/blog/unlocki...

I *see* you BSKY. Cleared #lightsheet #microscopy of the human #eye from Marie Darche in Paris and rendered in www.syglass.io.

Our 5th EMBO Practical course «Light Sheet Microscopy» will be held in Dresden, Aug 11-22nd. Registration open soon but you can already register interest. Organized with Marina Cuenca, Jan Peychl Pavel Tomancak and myself. Lots of scopes, expertise, friends and Data... coming-soon.embo.org/pc25-29

Axially Swept Light-Sheet Microscopy (#ASLM) synchronizes the camera’s rolling shutter with axial remote focusing (sweeping) of the light sheet. This enables 3D imaging with a thin optical section and isotropic resolution across large FOVs. Ideal for imaging cleared tissue! doi.org/10.1016/j.bp...

Hey nerds, you know about this, right? Spots are filling up. You can still register using the QR code. And I’ll be there with my lightsheet microscope. 🤸‍♀️🤸‍♀️🤸‍♀️

🌱 Boosting agriculture and plant science with cutting-edge tech! 🔬🪴☘️🍄🍌🌴🌿 3i Marianas microscope system offers high-resolution 4D imaging of Arabidopsis and other plants with a vertical stage (Spinning disk confocal + Sora + photomanipulation +lightsheet microscopy!)

Major update of #BigStitcher & #BigStitcherSpark: - 10x faster fusion - cloud support (S3 & GS) - improved interest-point alignment - worst error visualization - improved virtual image splitting - Zarr/N5/HDF5 export - consolidated menu structure #OSSI @HHMIJanelia @FijiSc

Plant Lightsheet Microscopy