Outstanding paper describing how SVAs are regulated,what's their function in gene regulation and its impact in cell fate Composite transposons with bivalent histone marks function as RNA-dependent enhancers in cell fate regulation | Cell #TEs #CellFate #HSCs #Aging www.cell.com/cell/fulltex...

Human whole epigenome modelling for clinical applications with Pleiades www.biorxiv.org/content/10.1... 🧬🖥️🧪

Thanks everyone involved in the work. Special thanks also to our amazing team of collaborators: @mercedesbarrero.bsky.social, Payer lab and Menendez lab

Thrilled to share with you the latest work from my PhD in Bigas lab at @researchmar.bsky.social @carrerasijc.bsky.social published in @bloodjournal.bsky.social We’ve programmed blood stem cells from PSCs using CRISPRa screen and found a new “bloody” cocktail #CRISPR #HSCs #StemCells #CellFate

Very happy to have our latest work on how IκBα regulates stemness independently from NF-κB. It has been a terrific collaborative work led by Lluís Espinosa and Daniel Alvarez-Villanueva @researchmar.bsky.social #nfkb #ikb #inflammation #stemcells www.cell.com/cell-reports...

H3K4 methylation instructs blood specification in vivo Bivalent chromatin instructs lineage specification during hematopoiesis | Cell #Hematopoiesis #CellFate #Chromatin www.cell.com/cell/abstrac...

(1/n) Thread @matteomazzocca.bsky.social @domenicnarducci.bsky.social Simon Grosse-Holz @jessematthias.bsky.social preprint Q: how does chromatin move? Using MINFLUX, SPT & SRLCI, we track chromatin dynamics across 7 orders of magnitude in time to provide answers www.biorxiv.org/content/10.1...

Nice review about alternative roles of nucleolus in genome organization in stem cells Emerging roles for the nucleolus in development and stem cells | Development #Review #Heterochromatin #GenomeOrganization #StemCells journals.biologists.com/dev/article/...

Lively conversations, shared laughs, and engaging networking during the breaks at #EMBLChromatin 🧬💬🤝

I am excited to share our lab's first review out with in Blood Cancer Journal. We discuss the impact of transposable elements on genome regulation and variation in both normal haematopoietic processes and haematological cancers. www.nature.com/articles/s41...

Review about principles of stem cell biology and cellular reprogramming approaches and their use for current and future therapeutic purposes Stem cells as role models for reprogramming and repair | Science #Review #StemCells #Reprogramming #RegenerativeMedicine www.science.org/doi/10.1126/...

Maternal H3K27me3 inheritance over de novo methylation shapes chromatin architecture The establishment of nuclear organization in mouse embryos is orchestrated by multiple epigenetic pathways | Cell #3DGenome #Chromatin #Development www.cell.com/cell/fulltex...

Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes | Science #TranscriptionFactors #GeneRegulation #Hematopoiesis www.science.org/doi/10.1126/...

The HuSH complex silences retrotransposable elements. We identify HuSH2, centered on TASOR2, targeting KRAB-ZNFs and interferon genes, balancing retrotransposon silencing and immune regulation. www.nature.com/articles/s41...

p300 acetylates METTL3 specifically on H3K27ac chromatin to promote transcription Spatial control of m6A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin | Molecular Cell #m6a #chromatin #transcription #mettl3 www.sciencedirect.com/science/arti...

The molecular basis of heterochromatin assembly and epigenetic inheritance | Molecular Cell #Heterochromatin #EpigeneticInheritance #Review www.sciencedirect.com/science/arti...

Our latest: uncovering hierarchical design rules for cis-regulatory elements with a high-throughput screen CRE activity follows multiplicative modular rules, tuned by spacing & positioning Enables engineering synthetic CREs for predictable tissue patterning www.biorxiv.org/content/10.1...

Changes in promoter histone acetylation and gene bursting are uncoupled Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens | Molecular Cell #GeneRegulation #Transcription www.cell.com/molecular-ce...

Transposon–host arms race: a saga of genome evolution | Trends in Genetics #review #transposon #HUSH #KRAB www.cell.com/trends/genet...

𝐇𝐨𝐰 𝐝𝐨 𝐜𝐞𝐥𝐥𝐬 𝐫𝐞𝐦𝐞𝐦𝐛𝐞𝐫 𝐰𝐡𝐨 𝐭𝐡𝐞𝐲 𝐚𝐫𝐞 𝐚𝐟𝐭𝐞𝐫 𝐃𝐍𝐀 𝐫𝐞𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧? Our new study “Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability” is out in 𝘚𝘤𝘪𝘦𝘯𝘤𝘦 𝘈𝘥𝘷𝘢𝘯𝘤𝘦𝘴. Led by @lleonie.bsky.social @biranalva.bsky.social 🧵 More below👇

Histone modifications occur independently of transcription RNA polymerase II coordinates histone deacetylation at active promoters | Science Advances #chromatin #transcription #generegulation www.science.org/doi/10.1126/...

Sequencing technologies to measure translation in single cells | Nature Reviews Molecular Cell Biology #Review #Sequencing #Translation #SingleCell www.nature.com/articles/s41...

Uncovering the whole genome silencers of human cells via Ss-STARR-seq | Nature Communications #Chromatin #GeneRegulation #Enhancers www.nature.com/articles/s41...

Linking RNA modifications to DNMT1 function on gene bodies Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiation | Cell #Epigenetics #DNAme #Development #DNMT1 #RNA www.cell.com/cell/abstrac...

Promoters have strong orientation-bias, but not the enhancers Massively parallel characterization of transcriptional regulatory elements | Nature #transcription #generegulation #chromatin www.nature.com/articles/s41...

Resolution of a human super-enhancer by targeted genome randomisation | BioRxiv #generegulation #enhancer #chromatin #preprint www.biorxiv.org/content/10.1...

In vitro synthesis of up to 96 nucleosomes. Amazing platform to study chromatin folding!! Gene-scale in vitro reconstitution reveals histone acetylation directly controls chromatin architecture | BioRxiv #chromatin #nucleosome #3Dchromatin #preprint www.biorxiv.org/content/10.1...

3D chromatin hubs as regulatory units of identity and survival in human acute leukemia | Molecular Cell #Chromatin #3DGenome #Leukemia www.cell.com/molecular-ce...

ZFN143 doesn’t affect 3D chromatin, but rather mitochondrial gene expression ZNF143 is a transcriptional regulator of nuclear-encoded mitochondrial genes that acts independently of looping and CTCF | Molecular Cell #TrancriptionFactors #Chromatin #Mitochondria www.cell.com/molecular-ce...

Chromatin-based memory as a self-stabilizing influence on cell identity | Genome Biology #Chromatin #CellFate #Review genomebiology.biomedcentral.com/articles/10....

More insights on how H3K9me restricts totipotency state Coordinated repression of totipotency-associated gene loci by histone methyltransferase EHMT2 through binding to LINE-1 regulatory elements | BioRxiv #Preprint #Totipotency #Pluripotency #Heterochromatin #LINE1 www.biorxiv.org/content/10.1...

How TFs recognize and invade new enhancers during cell fate transitions? By taking advantage of “signpost” 👇🏾👇🏾 Nucleosome fibre topology guides transcription factor binding to enhancers | Nature #Reprogramming #Chromatin #CellFate #Enhancers www.nature.com/articles/s41...

A knock-in of a 10bp heat shock promoter element in front of cell-wall-invertase genes improved heat stress response in rice and tomato!!! Engineering source-sink relations by prime editing confers heat-stress resilience in tomato and rice www.cell.com/cell/abstrac...

Inducing chromatin contacts in vivo triggers transgenerational epigenetic inheritance. Very cool result in flies! Interchromosomal contacts between regulatory regions trigger stable transgenerational epigenetic inheritance in Drosophila | Molecular Cell #Epigenetics www.cell.com/molecular-ce...

Linking topological dynamics to the magnitude of transcription Transient promoter interactions modulate developmental gene activation | Molecular Cell #3DGenome #Chromaton #StemCells #Differentiation #Development www.cell.com/molecular-ce...

Enhancer cooperativity can compensate for loss of activity over large genomic distances | Molecular Cell #chromatin #enhancers www.cell.com/molecular-ce...

A new method to nondestructively measure replication status and protein-DNA interactions on chromatin genome-wide. The single-molecule accessibility landscape of newly replicated mammalian chromatin | Cell #Chromatin #Replication #Transcription www.cell.com/cell/fulltex...

#Review The chromosome folding problem and how cells solve it | Cell #3DGenome #Chromatin www.cell.com/cell/fulltex...

Multiple ChIP-seqs in a row! ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements | Nature Genetics #Genomics #ChIPseq #ProteinDNAinteraction www.nature.com/articles/s41...

Look who's here @pnas.org ✔️ @science.org ✔️ @naturecellbiology.bsky.social ✔️ @natrevgenet.bsky.social ✔️ @naturebiotech.bsky.social ✔️ @naturemicrobiol.bsky.social ✔️ @naturechemistry.bsky.social ✔️ @genesdev.bsky.social ✔️ @cellchembiol.bsky.social ✔️ @genomeresearch.bsky.social ✔️ @jcellbiol.bsky.social ✔️

#CRISPR screens into the next level! They couple spatial transcriptomic with in vivo CRISPR screens Spatially Resolved in vivo CRISPR Screen Sequencing via Perturb-DBiT | BioRxiv #CRISPRtools #science #preprint www.biorxiv.org/content/10.1...

MultiPerturb-seq has arrived! Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling | Nature Biotechnology www.nature.com/articles/s41...

(1/8) 🚀 Excited to share our findings on a large-scale ChIP-seq assay for 166 previously uncharacterized human transcription factors (TFs) and their roles in both regulatory regions, and more strikingly, the “dark matter” genome. 🌌 doi.org/10.1101/2024....

EpiSci - www.cell.com/molecular-ce...

New here and excited to share our new paper on transcriptional effectors! Interested to discuss context-specificity of gene regulation? Or how to get CRISPRi/a working in new contexts? 👋 www.nature.com/articles/s41...

⚠️THREAD OF ALL SCIENCE-RELATED STARTER PACKS! ⚠️ 🔄Share to help people finding its topic/field - STRUCTURAL BIOLOGY Structural Biology 1: bsky.app/starter-pack... Structural Biology 2: bsky.app/starter-pack... Crystallography: bsky.app/starter-pack... Synchrotron&CryoEM: bsky.app/starter-pack...

SETDB1 activity is globally directed by H3K14 acetylation via its Triple Tudor Domain | Nucleic Acids Research #epigenetics #chromatin academic.oup.com/nar/advance-...

Very interesting study about the origin of SOX and POU transcription factors. This might shed light into the evolutionary origin of stem cells | Nature Communications www.nature.com/articles/s41...

Any recommended starting pack for science in BlueSky ??

Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells | Cell www.cell.com/cell/fulltex...