#Autism causes #Vaccines #Humour #ScienceHumour #MAHAha #LunaticRFKJr

@openvibe.social quite often, in light theme, I don't see my clock and other controls on top as the font is white on white background. But then sometimes I see it as the text becomes gray. Is it a bug from open vibe or android stuff?

Ms upgrade!!! +10% to IDs

There is something poetic in this email 🫠

@evcorefacilty.bsky.social @chpreusser.bsky.social

RIP... :(

How did I not think of this ? So insightful. So specific. Just leverage AI. (given the information density, multiple readings may help)

Starting today, only certified Evosep engineers will show up in the official orange cape and mask. Make sure your service hero is properly attired before letting them near your system! #proteomics #lcms

Our PCA-N workflow featuring a neutralization step allows direct enzymatic digestion, doubles proteome depth from 5µL neat plasma, and handles 10K+ sample-preps daily. Democratizing plasma proteomics with 50K sample validation. Check it out on bioRxiv! www.biorxiv.org/content/10.1...

For no particular reason at all, I want to give a shout-out to @signal.org - do yourself and your data privacy a favor and switch over from other messengers (tell your friends too!) Only way your data could be compromised there is if you accidentally invite a journalist to your group chat 👀

https://youtube.com/shorts/RnyUlMS5RAA?si=E3WNT-3aifshHkZn This race was a blast! Best Milan San Remo that I have watched. 🎉 And the commentary was 11/10💪 Rob does it best.

#teammassspec does anyone use 96-well plates from Macherey-Nagel with the HR-X material? Either the 85 or 45μm format? If not, can one recommend another plate for C18 based desalting? Ideally something suitable for small amounts of peptides.

I'm unfortunately not being paid by them but finally I found a better Bluesky client for Android: https://openvibe.social/ The main missing feature of the official app was the timeline scrolling and no ability to get back to reading where I left it last time.

I am late to the party, but what a f&%$king shit show it was from Trump and Vance during the meeting with Zelensky! It was an ambush, done on purpose in front of cameras! WTF? If I was Zelensky, I would not be able to keep my temper as he did... Jfc!

News in Proteomics Research blog post | Was your LC column provider acquired? Try a new vendor out, save some $$ and get a free column! proteomicsnews.blogs... --- #proteomics #prot-other

Favorite figure showing how poorly RNA and protein correlate?

DIA-NN 2.0 release is almost ready, will come with some big news. One is scanning methods support, at the moment doing some tuning of the algorithm using Synchro-PASEF data :) Turns out, 'Q1 information' is really helpful for gaining peptidoform confidence. Likely also channel-confidence in plexDIA.

From one side, it's a QC/QA issue that I got a broken PepSep column from #Bruker, but kudos to them for sending the replacement very quickly! What's your experience with other vendors? Do you pack the columns yourself or use commercial ones?

Another strange thing. If I run the method with the trap column, the spray seems to be fine. Same Hela QC without trap column, has spray issues.

1/2 #teammassspec I need help! My FAS @kronigert.bsky.social is on holidays and I have an issue. On TimsTof Ultra, coupled with nanoElute2 and using PepSep Ultra columns, I am loosing spray. I changed the column, I changed the emitter, today I exchanged the glass capillary, but for some reason...

I know that it's Christmastime and a common madness with packages, but... In the last 3 years, all 3 nearest post offices of #deutschepost were just closed. On Friday, I was waiting 35 minutes in the queue to send something. There was only one poor woman working there...

I have a radical idea. All DIA tool developers meet in Geneva for a convention and agree to apply certain additional conservative quality filters by default (so yeah, not 10k proteins but “just” 8k…). And then provide users an option to relax the filters at their own risk. Not the other way around.

@fcyucn.bsky.social Hi! Before I start a GitHub issue for FragPipe I thought I'd ask you shortly here. Can a non-specific digest search on dia-pasef data with a custom database of sORFs + Human be run? #teammassspec

#teammassspec is it only me that has a dedicated screen at home put on the remote to monitor my mass specs all the time or am I weird?

@chpreusser.bsky.social maybe something for you! Get those EV people for some knowledge exchange!

There is a disclaimer when you scan the QR-Code. They are built from biodegradable, food-contact-safe-plastic. Please hand-wash only!

Just got my TimsTof Ultra cookie form 😏 a nice touch from #bruker

I'm trying to get back here. One feature that is still keeping me away from #Bluesky is the inability to get on the feed and continue reading where I left it last time, and not scrolling from top to bottom to find the last read post. Is there a way around this that I don't know about?

#TeamMassSpec Any drawbacks of using DDM in your samples? The increase in the IDs in the lower injection spectrum is bonkers!!!

#bruker TimsTof Ultra is definitely seeing signal saturation with 50 ng of sample injection.

@fcyucn.bsky.social Any update on diaPASEF compatibility with FragPipe? :) You just tease me with new papers, now about the Analyst, but I can't use it as I measure everything with DIA on TT instruments. Greets

Testing goes further: Two weeks of Hela runs with BSA once per 4 samples. RT and IM and pressures stay steady on the PepSep ULTRA (the variation in the metrics mentioned above is random, and not related to the measurement time). #timstofultra #bruker #proteomics

2023 Hurricane Season: Look upon my sea surface temperature anomalies and despair! 2024 Hurricane Season: Hold my shear.

LIVE episode of #TheProteomicsShow with @benneely.com and Rena Robinson filling in for @proteomicsnews.bsky.social

Ok... I have to admit... I am a fanboy of Bruker's ProteoScape now. I can run my QC samples and adjust the method on the fly... Protein identification sitting ready a few minutes after the run is finished. This is SUPER helpful for any method development and QC purposes.

#TeamMassSpec has put TeamCryo on ice for the 4th year in a row and moved to the next round of the @jbiolchem #MethodsMadness tournament. Voting has started, let's go #TeamMassSpec and friends! www.asbmb.org/journals-new...

After a conversation with Bruker application specialist, the consensus looks like this: Bruker PepSep columns thread. @htlau.bsky.social

I laughed :D @benneely.com "Obviously, not new stuff, and this method was inspired by MacCoss lab's multiplexed PRM stuff that is so smart that no one aside from the authors have ever been smart enough to use it themselves."

@htlau.bsky.social hi! Can I contact you somehow in order to ask you some questions about your experience with the TT and Pepsep columns? Mainly interested in comparison of length Vs diameter Vs beads etc. If you tested many of them?

TimsTOF people, if you inject a peptide standard for 5 times, what would your peak area %cv be? I have been getting >20% for some peaks. Can it be the MS or it has to be the LC? The RT is reproducible. We have a Neo, running a 10 cm 150 ID Pepsep at 300 nL/min, inject 10 uL.

What happened on BlueSky with #teammassspec? I was away for like a month or two from the socials here. I was quite busy and just didn't have time to regularly check it. Now I come back and it's... It's kind of empty. Where is everyone? Back to TwitteX or Mastodon?

It's happening!!

Best New Year wishes I received so far :D Way to go #thermofisher :D

@bsky.app hi! This came out already many times I guess. Do you plan to add the "continue scrolling where I finished last time" feature? This is for me the most disturbing missing feature on Bluesky. Greets!! 💪

Wonderful thread summarizing a single-cell proteomics data analysis preprint from Laurent Gatto over on Mastodon. [preprint here www.biorxiv.org/content/10.1... ]

Anyone using uPac columns here? I would really love to get some hints, cause my peaks look awful. Here BSA: