James Connolly
@ruamicro.bsky.social
📤 875
📥 463
📝 15
MRC Career Development Fellow at Newcastle University. Studying how pathogens control their genes
pinned post!
Absolutely delighted to share the labs latest work. We identify and characterise two pathways for D-ribulose utilisation in pathogenic EHEC and Citrobacter.
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Convergent evolution of distinct D-ribulose utilisation pathways in attaching and effacing pathogens - Nature Communications
Cottam et al. identify distinct pathways for D-ribulose utilisation in pathogenic Escherichia coli and Citrobacter rodentium, providing mechanistic details and suggesting convergent evolution towards ...
https://www.nature.com/articles/s41467-025-62476-5
about 2 months ago
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James Connolly
Chris Stewart
2 months ago
Introducing a new gut microbiology journal, aptly titled Gut Microbiology. All Article Publishing Charges (APCs) are waived for 2 years! Please send your exciting research our way.
www.sciencedirect.com/journal/gut-...
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James Connolly
Westermann Lab
18 days ago
New preprint from the lab, in collaboration with Wenhan Zhu (U Vanderbilt): using dual RNA-seq during B. theta colonization of the host mucous layer, we identify IroR--an iron-response sRNA that tunes capsule expression and facilitates adaptation to iron limitation.
doi.org/10.1101/2025.09.08.672848
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An RNA regulates iron homeostasis and host mucus colonization in Bacteroides thetaiotaomicron
Symbiotic bacteria in the human intestinal microbiota provide many pivotal functions to human health and occupy distinct biogeographic niches within the gut. Yet the molecular basis underlying niche-s...
https://doi.org/10.1101/2025.09.08.672848
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James Connolly
José R Penadés
17 days ago
Thrilled to share our two latest papers with the
@tcostalab.bsky.social
lab! In the first, we uncover a new mechanism of satellite transfer: cf-PICIs hijack tails from diverse phages to spread across species.
@imperialcollegeldn.bsky.social
www.cell.com/cell/fulltex...
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Chimeric infective particles expand species boundaries in phage-inducible chromosomal island mobilization
Capsid-forming PICIs (cf-PICIs) produce their own capsids and exploit phage tails from unrelated species to transfer their DNA across bacterial hosts. This tail piracy enables broad dissemination and ...
https://www.cell.com/cell/fulltext/S0092-8674%2825%2900974-2
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James Connolly
José R Penadés
17 days ago
Our second paper with
@tcostalab.bsky.social
and GoogleDeepMind reports our experience with Google’s co-scientist: remarkably, the AI independently recapitulated our experimental discovery on cf-PICIs, showcasing AI’s potential to accelerate biology.
www.cell.com/cell/fulltex...
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AI mirrors experimental science to uncover a mechanism of gene transfer crucial to bacterial evolution
By solving a previously unsolved biological question, the AI co-scientist predicted a complex mechanism of gene transfer and generated hypotheses that opened new research directions, illustrating AI's...
https://www.cell.com/cell/fulltext/S0092-8674%2825%2900973-0
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James Connolly
Zeynep Baharoglu
21 days ago
So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary
@manonlang.bsky.social
with
@fox-science.bsky.social
&
@amazeld.bsky.social
+amazing collaborators
@immunobladder.bsky.social
@imaneelmeouche.bsky.social
🦠 We believe it can make a difference in
#AMR
infections!
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Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters
Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.
https://www.science.org/doi/10.1126/sciadv.adw7630
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James Connolly
Gavin Thomas (he/him)
25 days ago
Delighted with our new paper on a novel itaconate transporter in the human pathogen Pseudomonas aeruginosa, led by Javeria and Reyme.
@ybri-uoy.bsky.social
@javeriamehboob.bsky.social
@bethkw.bsky.social
A duplicated transporter on its way to itaconate selectivity
portlandpress.com/biochemj/art...
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Itaconate utilisation by the human pathogen Pseudomonas aeruginosa requires uptake via the IctPQM TRAP transporter
Pseudomonas aeruginosa PA01 is one of the major causes of disease persistence and mortality in patients with lung pathologies, relying on various host metabolites as carbon and energy sources for grow...
https://portlandpress.com/biochemj/article/482/17/BCJ20253132/236479/Itaconate-utilisation-by-the-human-pathogen
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James Connolly
Emmanuele Severi
about 2 months ago
www.biorxiv.org/content/10.1...
#microsky
#synbio
#ecoli
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A compact vector for scarless gene editing in E. coli
Here we present pCASKD, a single-plasmid system for scarless chromosomal editing in Escherichia coli. Our plasmid pCASKD integrates CRISPR-Cas9-mediated counterselection, Lambda-Red recombineering, an...
https://www.biorxiv.org/content/10.1101/2025.08.05.668820v1
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James Connolly
Microbiology Society
about 2 months ago
Applications for Outreach and Engagement grant are now open. Eligible members can apply for up to £1000 to support science outreach or public engagement initiatives. Check your eligibility and apply before 01 Oct 25 at 23.59 BST.
microb.io/OutreachandE...
.
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Outreach and Engagement Grant
Eligible members can apply for up to £1000 to support relevant science outreach or public engagement initiatives that engage new audiences in the art and science in any aspect of microbiology.…
https://microb.io/OutreachandEngagementGrant
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James Connolly
Katy Patras
about 2 months ago
Excited to share a new preclinical model, human bladder organoids, to study UTI, novel therapies, and beyond! Developed with Dr. Sarah Blutt, the BCM Organoid Core, and
@tailor-labs.bsky.social
www.biorxiv.org/content/10.1...
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Human bladder organoids model urinary tract infection and bacteriophage therapy
Urinary tract infections (UTIs), primarily caused by uropathogenic Escherichia coli (UPEC), are among the most common antibiotic-resistant infections. Despite this, currently available preclinical UTI...
https://www.biorxiv.org/content/10.1101/2025.07.30.667685v1
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Absolutely delighted to share the labs latest work. We identify and characterise two pathways for D-ribulose utilisation in pathogenic EHEC and Citrobacter.
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Convergent evolution of distinct D-ribulose utilisation pathways in attaching and effacing pathogens - Nature Communications
Cottam et al. identify distinct pathways for D-ribulose utilisation in pathogenic Escherichia coli and Citrobacter rodentium, providing mechanistic details and suggesting convergent evolution towards ...
https://www.nature.com/articles/s41467-025-62476-5
about 2 months ago
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reposted by
James Connolly
Julie A. K. McDonald
2 months ago
In case you missed it, check out our latest paper describing how vancomycin-resistant enterococci exploit antibiotic-mediated killing of gut commensals to grow in nutrient-enriched and metabolite-depleted intestines:
doi.org/10.1038/s414...
#AMR
#GutMicrobiome
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Vancomycin-resistant enterococci utilise antibiotic-enriched nutrients for intestinal colonisation - Nature Communications
Here, the authors show that vancomycin-resistant enterococci grow in the antibiotic-treated gut microbiome by utilising enriched nutrients in the presence of reduced concentrations of inhibitory micro...
https://doi.org/10.1038/s41467-025-61731-z
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James Connolly
Andreas Bäumler
2 months ago
Meet the new Editor in Chief of mSystems
journals.asm.org/doi/10.1128/...
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Humanity’s urgent challenges need solutions from systems microbiology | mSystems
It is with great excitement and sincere dedication that I assume the role of editor in chief of mSystems. Building on the momentum and reputation that mSystems has achieved in its first decade, we wil...
https://journals.asm.org/doi/10.1128/msystems.00838-25?utm_source=literatum
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James Connolly
Malcolm White
2 months ago
Jo Hobbs
@hobbslabmicro.bsky.social
has a 12 month Postdoc available on mechanisms of antibiotic tolerance. More details here:
www.vacancies.st-andrews.ac.uk/Vacancies/W/...
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James Connolly
Hannah Ledvina Ph.D.
3 months ago
Words cannot describe how excited I am to share the findings from the second half of my postdoc in
@aaronwhiteley.bsky.social
's lab where we discover that bacteria use functional amyloids to defend themselves from predatory bacteria.
rdcu.be/euu5Y
. See thread for details on this epic adventure 1/.
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Functional amyloid proteins confer defence against predatory bacteria
Nature - Escherichia coli uses curli fibres, oligomers of the functional amyloid CsgA, as a barrier to protect against the predatory bacteria Bdellovibrio bacteriovorus and Myxococcus xanthus in a...
https://rdcu.be/euu5Y
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James Connolly
Kimberly Kline 🏔
3 months ago
🔬Calling all microbiologists. 🔬 Ever feel like your paper is too small for some journals, but too solid to sit on?
#FEMSMicrobes
welcomes well-executed microbiology manuscripts, and they put special attention on promoting ECR authors, eg the webinar below. 👇
@femsjournals.bsky.social
@femsmicro.org
add a skeleton here at some point
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James Connolly
Willem van Schaik
3 months ago
I am delighted that our manuscript 'Staphylococcus haemolyticus is a reservoir of antibiotic resistance genes in the preterm infant gut' is now published.
www.tandfonline.com/doi/full/10....
Big thank you to all co-authors, particularly
@lisalamberte.bsky.social
and
@halllab.bsky.social
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Staphylococcus haemolyticus is a reservoir of antibiotic resistance genes in the preterm infant gut
Staphylococcus haemolyticus is an important cause of sepsis in preterm infants, with gut colonization being recognized as a risk factor for infection. To better understand the diversity of S. haemo...
https://www.tandfonline.com/doi/full/10.1080/19490976.2025.2519700
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James Connolly
Hall Lab
3 months ago
Amazing to see this now published! Congrats to
@lisalamberte.bsky.social
and
@wvschaik.bsky.social
- on leading this important
#microbiome
study on
#AMR
and Staphylococcus haemolyticus in the preterm gut 👇
add a skeleton here at some point
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James Connolly
Westermann Lab
3 months ago
Our latest:
www.cell.com/cell-reports/fulltext/S2211-1247(25)00615-1
. Links Bacteroides cell size w/ gene expression. Protocol works even for single cells. 🦠 Shoutout to
@elisebor.bsky.social
,
@emmanuel-saliba.bsky.social
,
@lbarquist.bsky.social
,
@jorg-vogel-lab.bsky.social
, Till Strowig & KC Huang.
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Low-input RNA-seq suggests metabolic specialization underlying morphological heterogeneity in a gut commensal bacterium
Bornet et al. investigate the morphological heterogeneity of Bacteroides thetaiotaomicron cells observed both in laboratory culture and inside the mammalian GI tract. The authors show that cell size i...
https://www.cell.com/cell-reports/fulltext/S2211-1247(25)00615-1
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James Connolly
Jan-Willem Veening
3 months ago
Some positive news in troubled times: New preprint by Vincent de Bakker, Xue Liu
@jonbakerlab.bsky.social
Infection-mimicking conditions+transcriptomics/proteomics/'essentialomics'/lipidomics shows many cool things!
www.biorxiv.org/content/10.1...
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Multi-omics profiling reveals atypical sugar utilization and identifies a key membrane composition regulator in Streptococcus pneumoniae
The human body comprises many different microenvironments, each with their own challenges for microorganisms to overcome in order to survive, and possibly cause infection. The human pathogen Streptoco...
https://www.biorxiv.org/content/10.1101/2025.06.13.659575v1
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James Connolly
Rebecca McHugh
5 months ago
Absolutely delighted to share that I've been awarded the BBSRC Fellowship to continue my working on antivirulence drugs at the University of Glasgow. A massive thank you to all of my mentors and collaborators. I can't wait to see what the future holds for the project 🔬
add a skeleton here at some point
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James Connolly
Prof Jenny Rohn
6 months ago
🔈🎉 I will soon be advertising a postdoc in my friendly London lab, funded by MRC
@ukri.org
, to decipher uropathogen virulence and host response pathways in our 3D-UHU human urothelial model! A great collaboration with co-Is
@gpollara.bsky.social
and
@scottishwormboy.bsky.social
#UTISky
#UTI
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James Connolly
Professor Andrew J Roe
6 months ago
New paper from the group - here we show that aurodox prevents shiga toxin mediated damage in a murine model. Interesting changes to the microbiota too. Amazing work by
@rebeccamchugh.bsky.social
and the group. Paper here-
rdcu.be/efW0K
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James Connolly
Andreas Bäumler
7 months ago
🔬🦠 How to make sense of the taxonomic complexity & interpersonal variability of the gut microbiota? We propose that beneath this complexity lies a hierarchy of factors that control gut microbiota assembly. Read more!
#Microbiome
#GutHealth
authors.elsevier.com/a/1km32_,2Ci...
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James Connolly
Dr Katherine Duncan
6 months ago
One week left to apply for this BBSRC funded PhD position in the Duncan lab, cosupervised by
@thecopperdoctor.bsky.social
and
@ruamicro.bsky.social
www.findaphd.com/phds/project...
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Antibiotics from the abyss – assessing the impact of deep-sea mining on biodiscovery at Newcastle University on FindAPhD.com
PhD Project - Antibiotics from the abyss – assessing the impact of deep-sea mining on biodiscovery at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/project/antibiotics-from-the-abyss-assessing-the-impact-of-deep-sea-mining-on-biodiscovery/?p182723
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James Connolly
CBCB Newcastle
6 months ago
Antibiotics from the abyss; assessing the impact of deep-sea mining on biodiscovery. Supervised by
@kateduncan.bsky.social
,
@thecopperdoctor.bsky.social
and
@ruamicro.bsky.social
.
www.findaphd.com/phds/project...
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Antibiotics from the abyss – assessing the impact of deep-sea mining on biodiscovery at Newcastle University on FindAPhD.com
PhD Project - Antibiotics from the abyss – assessing the impact of deep-sea mining on biodiscovery at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/project/antibiotics-from-the-abyss-assessing-the-impact-of-deep-sea-mining-on-biodiscovery/?p182723
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James Connolly
CBCB Newcastle
6 months ago
Microbial metal-head: the integrated roles of metal homeostasis in host-pathogen interaction. Supervised by
@ruamicro.bsky.social
,
@thecopperdoctor.bsky.social
and Manuel Banzhaf.
www.findaphd.com/phds/project...
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Microbial metal-head: the integrated roles of metal homeostasis in host-pathogen interaction at Newcastle University on FindAPhD.com
PhD Project - Microbial metal-head: the integrated roles of metal homeostasis in host-pathogen interaction at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/project/microbial-metal-head-the-integrated-roles-of-metal-homeostasis-in-host-pathogen-interaction/?p182732
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Still time to apply for this PhD position with our team!
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6 months ago
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Fully funded PhD position available to join our team! We will focus on untangling the molecular mechanisms used by pathogenic, antibiotic resistant E.coli to colonise the host gut. Highly interdisciplinary project in collaboration with
@thecopperdoctor.bsky.social
and Manu Banzhaf. Please share
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Microbial metal-head: the integrated roles of metal homeostasis in host-pathogen interaction at Newcastle University on FindAPhD.com
PhD Project - Microbial metal-head: the integrated roles of metal homeostasis in host-pathogen interaction at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/project/microbial-metal-head-the-integrated-roles-of-metal-homeostasis-in-host-pathogen-interaction/?p182732
7 months ago
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James Connolly
Giusy Mariano
7 months ago
We have extended the deadline for this! If you are passionate about phage-bacteria interactions, check this out and join us!
add a skeleton here at some point
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Brilliant opportunity to work with Alex and my team on our collaborative project. Don’t hesitate to apply!
add a skeleton here at some point
7 months ago
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reposted by
James Connolly
Tracy Palmer
8 months ago
Fab new study from
@ruamicro.bsky.social
and team!
add a skeleton here at some point
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reposted by
James Connolly
Andy Goodman
8 months ago
Starting my Bluesky adventure by highlighting a new paper from the lab!
add a skeleton here at some point
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James Connolly
Zeynep Baharoglu
8 months ago
Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination
#microsky
🦠
#bmsv
from
@tunglejic.bsky.social
lab
www.biorxiv.org/content/10.1...
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Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination
Gene Transfer Agents (GTAs) are domesticated prophages that cannot self-multiply and be infectious but might have been co-opted to perform biological functions for the host bacteria. Caulobacter cresc...
https://www.biorxiv.org/content/10.1101/2023.11.12.565669v2
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James Connolly
Giusy Mariano
8 months ago
🚨We are hiring! A MRC-funded PDRA position to study novel anti-phage systems & their evolutionary dynamics in P. aeruginosa, in collaboration with @friendlymicrobe. If you are passionate about phage-bacteria interactions & evolution, apply now !
tinyurl.com/bddz4z44
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James Connolly
Lauren Radlinski
8 months ago
Microbiome science needs more microbiologists- A new commentary authored by myself and
@abaumler.bsky.social
www.nature.com/articles/s41...
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Microbiome science needs more microbiologists - Nature Microbiology
Microbiome science is a multi-disciplinary field, but classical microbiologists are needed to ensure advances are grounded in our understanding of basic microbiological concepts.
https://www.nature.com/articles/s41564-024-01922-4
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James Connolly
Kate Beckham
9 months ago
First post of the year to mark my return from maternity leave :) Excited to share the first open PhD position in my group. Deadline end of February. Please spread the word.
www.findaphd.com/phds/projectdetails.aspx?PJID=181239
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PhD studentship: Structural and functional characterisation of mycobacterial outer membrane proteins at Newcastle University on FindAPhD.com
PhD Project - PhD studentship: Structural and functional characterisation of mycobacterial outer membrane proteins at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/projectdetails.aspx?PJID=181239
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James Connolly
Grainger Lab
8 months ago
If you're interested in chromosome biology (DNA folding, gene regulation, replication, polymer modelling and more) we're organising the below this autumn. It'd be great to see you there. Please share if you can. :-)
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Great opportunity to work with Kate here at Newcastle, apply now!!!
add a skeleton here at some point
9 months ago
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James Connolly
Lars Barquist
9 months ago
Latest from the Westermann lab investigating the RNA biology of the human gut commensal B. thetaiotaomicron -- the RNA-binding protein RbpB regulates polysaccharide utilization through interactions with 14 (?!) paralogous sRNAs 🧪🦠
#RNASky
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The global RNA-binding protein RbpB is a regulator of polysaccharide utilization in Bacteroides thetaiotaomicron - Nature Communications
Gut Bacteroides deploy several polysaccharide utilization loci (PULs) to forage diverse dietary and host-derived glycans. Here, the authors identify the RNA-binding protein RbpB and a family of noncod...
https://www.nature.com/articles/s41467-024-55383-8
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James Connolly
Eduardo Rocha
9 months ago
To start the year on the good path. Our preprint on plasmid-chromosome cross-talk and how it may favour MDR bacteria, is now out. Work led by
@sanmillan.bsky.social
Laura Toribio-Celestino et al.
add a skeleton here at some point
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James Connolly
Leibniz Institute DSMZ
9 months ago
Honouring Susan Gottesman’s major contributions in the field of microbiology, biochemistry & molecular biology, DSMZ researchers named the strain Streptomyces gottesmaniae (DSM 3412) after her.
#herstory
#HonoringWomenInSTEM
#WomenInScience
#antibiotics
#mRNA
#streptomycete
#microbiology
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James Connolly
Tracy Palmer
11 months ago
Funded PhD available in my lab. Pls repost!
www.findaphd.com/phds/project...
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PhD studentship: Staphylococcus aureus interactions in cystic fibrosis polymicrobial infections at Newcastle University on FindAPhD.com
PhD Project - PhD studentship: Staphylococcus aureus interactions in cystic fibrosis polymicrobial infections at Newcastle University, listed on FindAPhD.com
https://www.findaphd.com/phds/project/phd-studentship-staphylococcus-aureus-interactions-in-cystic-fibrosis-polymicrobial-infections/?p176969
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James Connolly
Tracy Palmer
11 months ago
Delighted that our ERC Synergy grant CombaT7 has been funded! Third time we’ve interviewed so the persistence paid off
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James Connolly
Michael Brockhurst
10 months ago
2 exciting PhD opportunities: - with
@fionawhelan.bsky.social
understanding evolution of transmissibility in Pseudomonas
www.findaphd.com/phds/project...
- with
@claudiaigler.bsky.social
Patrick Cai on AI-powered phage genome design
www.findaphd.com/phds/project...
Please spread the word!
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[FSE Bicentenary PhD] Generative AI approaches for synthetic phage genome engineering at The University of Manchester on FindAPhD.com
PhD Project - [FSE Bicentenary PhD] Generative AI approaches for synthetic phage genome engineering at The University of Manchester, listed on FindAPhD.com
https://www.findaphd.com/phds/project/fse-bicentenary-phd-generative-ai-approaches-for-synthetic-phage-genome-engineering/?p178755
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James Connolly
Grainger Lab
10 months ago
PhD projects available in our lab all listed in the below link. All focus on bacterial gene regulation, in the context of basic understanding, disease, or antibiotic resistance. Deadlines in January...
www.findaphd.com/phds/?Keywor...
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grainger birmingham PhD Projects, Programmes & Scholarships
FindAPhD. Search Funded PhD Projects, Programmes & Scholarships in grainger birmingham. Search for PhD funding, scholarships & studentships in the UK, Europe and around the world.
https://www.findaphd.com/phds/?Keywords=grainger+birmingham
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James Connolly
Lars Barquist
10 months ago
We recently built an interactive 3D exploration tool for bacterial
#SingleCell
RNA-seq, led by a master's student in design hosted by my group. Short video trailer below highlighting some of the features, or check out the paper for more details! 🧪🦠🧫🧬🖥️
academic.oup.com/nargab/artic...
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sCircle - Teaser
YouTube video by Max Seeger
https://www.youtube.com/watch?v=eoBJmHXsp9w
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James Connolly
ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ
10 months ago
in lively festivals each spring throughout Catalonia (Spain), microbiology professors and their postdocs thrill crowds by reenacting the assembly of a type 3 secretion system (T3SS)
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James Connolly
Melanie Blokesch
10 months ago
Exciting times! Our latest preprint is now live 🤩! After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...
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Diverse phage defence systems define West African South American pandemic Vibrio cholerae
Our understanding of the factors underlying the evolutionary success of different lineages of pandemic Vibrio cholerae remains incomplete. Interestingly, two unique genetic signatures define the West ...
https://www.biorxiv.org/content/10.1101/2024.11.23.624991v1
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James Connolly
ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ
10 months ago
Conversation with the brilliant contemporary microbiologist and molecular biologist—HHMI and MacArthur Fellow Bonnie Bassler of Princeton University Pioneer in the chemical signaling mechanisms that bacteria use to communicate with each other known as “quorum sensing”
www.hhmi.org/news/tapping...
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Tapping into Bacterial Conversations | HHMI
Over the course of 30 years, HHMI Investigator Bonnie Bassler has helped usher in a new branch of science centered on quorum sensing, the process by which bacteria communicate with one another and orc...
https://www.hhmi.org/news/tapping-bacterial-conversations
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James Connolly
Petra Dersch
10 months ago
Great new article from the Bäumler lab Salmonella virulence factors induce amino acid malabsorption in the ileum to promote ecosystem invasion of the large intestine | PNAS
www.pnas.org/doi/10.1073/...
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PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
https://www.pnas.org/doi/10.1073/pnas.2417232121?utm_source=TOC&utm_medium=ealert&TOC_v121_i47=&ref=d23387
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