It is an honor to inform you that our manuscript with low resolution FAIMS is now online www.biorxiv.org/content/10.1...

We’re excited to present this integrative analysis of single-cell proteomics and transcriptomics of the human HSPC hierarchy. Now published in @science.org together with @fabiantheis.bsky.social, @erwinschoof.bsky.social, @porsebo.bsky.social. www.science.org/doi/10.1126/... 🧵

Start of day 2 of the European Symposium on Single Cell Proteomics with @ryankellybyu.bsky.social telling us about high throughput proteomics towards a $10 proteome #ESCP2025

Mikaela now tells us about the scverse community intrageted ecosystem for single cell with established templates and data structures #ESCP2025

Wilfred Rossol is next talking about proteomic profiling of protein aggregates with a more spatial proteomics approach #ESCP2025

Its exciting to see that its a full house at #ESCP2025 lots of enthusiastic single cell proteomics researchers 🤓

The last session of the day is opened by Tami Geiger looking at the interactions of the immune cells with the cancer microenvironments using single cell proteomics #ESCP2025

Alexander Ivanov talking about increases in sensitivity and signal to noise when changing to ultra low flor rates for single cell proteomics. #ESCP2025

Seriously pumped for today! Deep Visual Proteomics masterclass here we come 🤓

Great from from @erwinschoof.bsky.social and his lab showing the added value of SCP!

On my way to ESCP. Super looking forward to this awesome meeting!

Only a few more hours! 😃

Start of the European symposium in single cell proteomics at beautiful Viena with a workshop on spatial proteomics organised by @coscialab.bsky.social

After the DVP symposium now begins the start of the European Symposium on Single Cell Proteomics #ECSP25 A quick intro from @kmechtler.bsky.social and we kick off two days of exciting science starting with SCP of neutrophils in glioblastoma

Next up is @vadim-demichev.bsky.social telling us how quantitatively accurate is single cell proteomics #ESCP2025

Cool work from @nanopots.bsky.social doing cell surface profiling of 100-500 immine cells using affinity purification #ESCP2025

Daniela Valdes from @coscialab.bsky.social lab presents how smoking disrupts the placental function via macrophage dysregulation using single nuclei analysis and deep visual proteomics #ESCP2025

After a long trek from Melbourne, Jarrod is now on the ground in Vienna and enjoying #ESCP2025 - together with Joris, our new Business Development Manager for Europe. Stop by the IonOpticks stand and chat with Jarrod and Joris about how our new product releases can support your single-cell work.

Up next is @jwoessmann.bsky.social from the @erwinschoof.bsky.social lab showing how to focus on the exciting lower abundance proteins with single cell proteomics #ESCP2025

Next up is @pedroaragon.bsky.social talking about single cell and low input proteomics to study perturbation in leukemia #ESCP2025

Thanks to all who helped make this possible: the EMERGE team, research nurses, clinical & research collaborators & patients. @kmechtler.bsky.social @pranvera.bsky.social @patriciacoelho.bsky.social @ajmhowden.bsky.social @mas-g.bsky.social @walmsley-lab.bsky.social @edinuni-irr.bsky.social

#ESCP25: The first scientific abstracts have been submitted, and I can only say one thing—it's going to be incredibly exciting. Enclosed can you find one of our highlights presented by Ying: "Affinity purification mass spectrometry: the next frontier of single-cell and low-input proteomics".

Breaking ESCP25 news: Ahead of the ESCP conference we will offer a Deep Visual Proteomics (DVP) workshop on August 25th 2025. Participation is free of charge, but registration is required! (www.apma.at/6thescp) Enclosed can you find the preliminary program:

I would like to inform you that the registration deadline for our ESCP25 conference is July 27th. We are looking forward to interesting scientific contributions.

The European Symposium on Single Cell Proteomics (ESCP) will be held in Vienna for the 6th time, from August 26th – 27th 2025 This year's focus will include spatial proteomics studies on the single-cell level. There is no registration fee Hotel rooms in Vienna are inexpensive. www.apma.at/6thescp/

Great presentation! Just FYI: It is a little known fact that the MS1 quantification by CHIMERYS on the MSAID Platform works by deconvoluting isotope envelopes in a similar way as JMod since the inception of the platform a year ago. If you are interested in 9-plex DIA support, feel free to reach out!

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I think we agree with each other since I said that it coats the TIMS instead of the quad? Definitely not something that can be serviced without venting but I was unaware that only Bruker can clean the TIMS cell.

A few years ago I reported on the cleaning cycles of a Thermo instrument (Q-Exactive HF-X). This is particularly important in core facilities where high throughput and consistent quality are required. Now I would like to report briefly on the OrbiTrap Astral: Installation was done 1 year ago!

Excited to unveil our new, efficient & easy to implement workflow for in vivo XL-MS! 🎉 It delivers a comprehensive PPI network already from a single fraction measurement, saving valuable MS time and resources. www.biorxiv.org/content/10.1...

Lovely work from @jbubis.bsky.social @matzingerm.bsky.social and @kmechtler.bsky.social

I dislike the name of the instrument being in the title, but otherwise this is amazing. Over 5,000 proteins from one (large) A549 cell? At 50 cells/day? Ain't cheap in $$/cell but 2-fold quan appears detectable in the sub nanogram peptide load range?? proteomicsnews.blogspot.com/2025/01/up-t...

How quickly time flies? Incredibly grateful and in awe to be working at one of the best places for science worldwide, the Vienna BioCenter. I am looking forward to new, exciting but also challenging projects. And if anyone wants to write a research proposal, we would be honored to provide support.

With this manuscript we would like to demonstrate the incredible power of the Orbitrap Astral for XL-MS. Over 40% more IDs than the Orbitrap Eclipse! This opens up a completely new dimension for XL-MS. www.biorxiv.org/content/10.1...

This problem used to exist and was solved a few years ago.

For the installation itself, isn’t it just a pass/fail set of specifications? I don’t know what Thermo could have been required to do if you saw that one instrument wasn’t performing as well as the others.

The cat is out the bag: SCP meets Spatial Proteomics, the Nature Method of the year 2025. lnkd.in/dkrxhuF8 Registration is open, seats are free of charge but limited (first come, first serve). www.apma.at/6thescp/

It gives me great pleasure to announce our next European Single Cell Proteomics Conference www.apma.at/6thescp/ We would be delighted to receive many abstracts from students so that they have the opportunity to give their first presentation. On behalf of: Erwin, Fabian, Manuel, Fabian and Karl

proteomicsnews.blogspot.com/2024/11/astr... I need some time to digest ben's blog. And would like to contribute my know-how or my opinion with much respect for Ben. EvoSep: the critical point is how the sample is applied to the tips. How long is centrifuged. Does centrifuging dry out the sample.